LOCUS       sequence-21772-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Banks CA, Kong SE, Spahr H, Florens L, Martin-Brown S, Washburn MP, 
            Conaway JW, Mushegian A, Conaway RC
  TITLE     Identification and Characterization of a Schizosaccharomyces pombe 
            RNA Polymerase II Elongation Factor with Similarity to the Metazoan 
            Transcription Factor ELL.
  JOURNAL   J Biol Chem. 2007 Feb 23. 282(8):5761-9.
  PUBMED    17150956
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Feb 23. 282(8):5761-9."
COMMENT     Sequence Label: FLAG-SpEAF 60-251 pBacPAK8
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctgtttcgt acagttttgt ataaaaaaac ctataaatac catgggtcat catcatcacc
       61 atcacgggtc gacaagcttg gatccctgca ggcctatgcc agactacaag gacgatgatg
      121 acaagctcga gatggacttt tatctattaa atgatgatga caggcctcat atatttgaag
      181 gctcatgcca acgagccagg aatgtggact gtgtactaat ttttaacgct aaaacaaaga
      241 cattcacact ggagcatatt gatgagattg ctcgattaaa tgctttacgc aatccaaaag
      301 tctctaaaac tgtaccttct aatgccatca ctcaatctga taattcccaa atctctgaat
      361 ccaaatcgac ctctcaaagt gcggtcacga ctaattccac gagacgtaaa gaaaaggaat
      421 tggaagcttc aaaggatgga aaaataaaac cttcatcgag caatactcga tatcctgcca
      481 tatctagcaa gggaccaatc accaccgata ccaacgatga gcctgacatg gaggtcatgg
      541 agttggatga ctttgccaaa gagttagagc taggatttga tcaggaattt aattccatag
      601 acgacccctc tacagtatcc caaacggcca gcaaaccaat ctctttgagg ggtctatctt
      661 cgca
//
