LOCUS       sequence-217723-        7953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of GST and full-length Drosophila SUUR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Nordman JT, Kozhevnikova EN, Verrijzer CP, Pindyurin AV, Andreyeva 
            EN, Shloma VV, Zhimulev IF, Orr-Weaver TL
  TITLE     DNA copy-number control through inhibition of replication fork 
            progression.
  JOURNAL   Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10.1016/j.celrep.2014.10.005. 
            Epub 2014 Oct 30.
  PUBMED    25437540
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.10.005"; journalName: "Cell Rep"; date: 
            "2014-11-6- 6"; volume: "9"; issue: "3"; pages: "841-9"
COMMENT     Sequence Label: pGex4t2 with full-length SUUR
FEATURES             Location/Qualifiers
     source          1..7953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        833..910
                     /gene="lacI"
                     /label=lacI promoter
     CDS             911..1993
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(930..949)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(2042..2047,2048..2065,2066..2072)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2080..2096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2104..2120
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2104..2120
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2116..2643
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(2136..2153)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2136..2152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2145..2167)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(2745..2750,2751..2766,2767..2773)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2781..2797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2820..3473
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     3431..3453
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             3480..3497
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(6565..6587)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6732..6750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6818..6922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6923..6991,6992..7783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7141..7160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt ccgacaccat
      841 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
      901 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      961 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
     1021 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     1081 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
     1141 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
     1201 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
     1261 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
     1321 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
     1381 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
     1441 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
     1501 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
     1561 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
     1621 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
     1681 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
     1741 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
     1801 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1861 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1921 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1981 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcaccccagg
     2041 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2101 acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt
     2161 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2221 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2281 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
     2341 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
     2401 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt
     2461 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
     2521 caggaaggcc agacgcgaat tatttttgat ggcgttggaa ttagcttatc gactgcacgg
     2581 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
     2641 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
     2701 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
     2761 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagtattca
     2821 tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt
     2881 tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat
     2941 ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg
     3001 gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca
     3061 tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg
     3121 atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg
     3181 attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa
     3241 catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg
     3301 ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa
     3361 aacgtattga agctatccca caaattgata agtacttgaa atccagcaag tatatagcat
     3421 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatc
     3481 tggttccgcg tggatccatg tatcactttg tatccgagca aacgccggag gtgcggctca
     3541 cagatgaggc actggtcacg agccacgtga cgcagtacct caagagcttt caactggatg
     3601 ccgtccgctt cgtctacgat cgtttggcta agcgggaatt ctgcatcctg aatgatgaga
     3661 gtggccttgg aaaggtcgct acggtggcgg cacttctaag tgccctacca cccgccaaaa
     3721 aaacacttgt ggtgctccag aacgatgagc aactgctcac cggttggcga ttccatttgg
     3781 acactctcac tgacctccag gtgtacatca ttcagggggt gcaagatacc accgactccc
     3841 cgcacagcgt ttacctggcg aaatggagcc aactacggag cattggagat ctcagtcgcc
     3901 tcaagttcga ctacattatg gtggataatc gggggcattc gctgaacaac agcttttgca
     3961 cttccatgct gctgaagcaa ttcgagggac gggtaaacgt tcttatctcc agtgttgacg
     4021 ttacgtccga cgttaggttg ctctacaatg ttctacggtt gggcggccga ctggagcatc
     4081 agtacaaaag ctttgcgagc ttcgatcgaa aattccatct gccagatccg aaggaggtat
     4141 tcagtaagcg catagatctc gaggaatact acaagcagcg gggattcctc agcgagtaca
     4201 tcaaggactt tcgtctgcgt cgttttcgtc atcaattcga caagtcactg ccacttgtcg
     4261 caccggagca atacaaacac aatctgaatc tctggttggc ctcaaagaac agccagagca
     4321 caataagcgg ctcagatgtg tgctctacaa tcgcttccat agacaacaat ccggctcagc
     4381 aaaataaaac tgggctcttt gaagaaacgg ataggttatc cgagcacagc gtggatgatg
     4441 tggcaatgtc accgctgata ttcgagtatt ccgagtctga cgatgaaccc ctaacagtag
     4501 aacctgatgc agatcaaaac ccggtattag ttgtatccag cgatgattgt gagattgtaa
     4561 cacctccaag tactccgcaa aatcgaacgc cctcattaaa tgaatcacca aggacgaaat
     4621 caaagaaaaa atttagcaaa aaaacgtcac cgcgcaaaaa agctgatctc accgattccg
     4681 aagaggatga tgaagcgacc gataatatgc cgccaaggaa gagaactcgt gcagccaccg
     4741 ttcatctgac acctaagaca aggagactca atgttcgaat tttaagggtt tccctcgata
     4801 ctctgagcac accaccaccg tccagaacca ctactgcgat cgtaacgccc aaaacggagc
     4861 ccacagctag aagaaaaaat ctgaaaaagc gcacggtgag cccagtggat gtgggtcgtc
     4921 cagccacaag gggaatgcag cgcctaacac gatccgctga aacaaaaata aacagtaagt
     4981 acttgaaaca tcgctcattg gatgatgtca agcgtagctt tccaaggaga gtcaagttgg
     5041 agggtaacca aacgcccaga agtagcaaac aaatagtgaa gcaggagcct aagtctaaag
     5101 tcgggcagga aaagaaacag aagacggtgg atgtacctgc tcaaggaact gcgaaacgaa
     5161 agcctggacg tccgagaaaa tgccaaacca agactgaaga tttggggaag acaaagacta
     5221 agccgaattc aaagcacttg ccacctactc ctcaagttct cagtggtagc tcgttgtcct
     5281 cggagtacat gcaatgtgct cagcgaatac cagacaattt ggatgccatt gagtcgcccg
     5341 cttttcgtgt gccctttaca cctcaacaaa ctccaatgct tctaaccttg ccgtctacac
     5401 acaatttgct caacgattct gaggtggtgt ctattccgct ttacaaagat ccagttgaga
     5461 cagtggtgat caatagctcg cacgatgagt gttctccgca ggatccatcg cagagtcgtc
     5521 gcaccaaggc tttgaaaaga aaacgcaaac ccgtgacgtc cgtcaattca agtttcggcg
     5581 gcggattggg tctaccaccg gccaagagga gtgccaacaa atctccagat ctatttagca
     5641 tatcctcgga acactcgcag attccgctgg cgcaaccaag accctcatcg cccttcgaag
     5701 gctttaagat ctttggctct gaggtgaagc agtttcagca gcagctagcg aaagtaaata
     5761 tatcggtgcc aaaaaagaag cgagatcgct cgtgtttgga tatcctggag caaatgttcg
     5821 agccacgaca acagcagtcg gctaaaacta gtcccaaagt cttgccaacc ttgccactga
     5881 cccagaagga tgatgcagag tctaccatta cgcagagaag aagaaccctg ctggaagatg
     5941 acttctttga gataacgaac aatggccaat ttggcagtcg catgcgattg aactcctctg
     6001 gcgaagtatc gcccgtgcaa ccggatcaac agtccgtcag gccgtcgcag gccaataaaa
     6061 tcaccaatta tttgataggt tctggaatta cgcaggagcg aacgcaacct tcgaatggta
     6121 atcgcaattc cattcttgca tcgctgcgca agtcacccaa atctcccaaa cagggtgcta
     6181 aatccactca ggccacgaaa ctaacacgct ggtttggctc cgtcttcggc ggtggagcct
     6241 cacagaccag ctccgtggaa tcggttagtg caccaagtac gccggtgaat tcatccacaa
     6301 gtgcagcagc ttgtcaaacg cgatcggcga gaagtggtgg agcatcagga ccgaccaaaa
     6361 gaaagcgatt ggaactgttc aagtgatgaa cagcaatcaa tgaaaatgac aaaaaccaaa
     6421 tcaaatcaaa tccaattatt gaatatataa tgaatgaagg aatatataac atatagtacg
     6481 aaatcaaaag ttgggtcgac tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc
     6541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     6661 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt ataattcttg
     6721 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     6781 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6961 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7021 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7081 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7141 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     7201 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7261 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7321 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7381 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7441 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     7501 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7561 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7621 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7681 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7741 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7801 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7861 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7921 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
