LOCUS       sequence-217728-       10500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing MS2p65HSF1 under the control of the 
            long human EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Chong ZS, Ohnishi S, Yusa K, Wright GJ
  TITLE     Pooled extracellular receptor-ligand interaction screening using 
            CRISPR activation.
  JOURNAL   Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
  PUBMED    30477585
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-11-26"; volume: "19(1):205. doi"; pages: " 
            10.1186/s13059-018-1581-3"
COMMENT     Sequence Label: pKLV2-EF1aMS2p65HSF1hyg-W
FEATURES             Location/Qualifiers
     source          1..10500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(297..314)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    303..527
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(553..573)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(553..571)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(592..608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(592..608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(605..627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    616..632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(640..646,647..664,665..670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    685..706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(823..840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(994..1582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1074..1093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1753..2544,2545..2613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2376..2395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2614..2718)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2744..3199)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2881..2902)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3093..3112
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3326..3348
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3340..3357
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3341..3357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3367..3386
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3367..3385
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3701..3899
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3854..3874
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3917..4097
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4144..4269
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4768..5001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5186..5230
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5528..5645
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6116..7297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6347..7289
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7246..7266
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7319..7328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7328..7714
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             7769..7789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7805..8347
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             8372..8743
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             8759..8812
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8816..9835
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     primer_bind     complement(9916..9936)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10334..10345)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa gtcaagatct
       61 gctttttgcc tgtactgggt ctctctggtt agaccagagt ctctctggtt agaccagatc
      121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      181 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      241 ctcagaccct tttagtcagt gtggaaaatc tctagcagtt taaacccgct gatcagcctc
      301 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      361 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      421 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
      481 ttgggaagtc aatagcaggc atgctgggga tgcggtgggc tctatgggcg gccgttaatt
      541 aagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat
      661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     2761 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2821 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     2881 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2941 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3001 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3061 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3121 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3181 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3241 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     3301 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     3361 cgcgcgtaat acgactcact atagggcgaa ttgactagta cataacttac ggtaaatggc
     3421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3481 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3541 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     3601 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     3661 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     3721 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3781 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3841 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     3901 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3961 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4021 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4081 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     4141 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     4201 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     4261 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     4321 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     4381 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     4441 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     4501 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     4561 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgcgct
     4621 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     4681 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     4741 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     4801 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     4861 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     4921 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     4981 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     5041 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     5101 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     5161 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     5221 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     5281 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     5341 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     5401 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     5461 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     5521 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     5581 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     5641 attttcgggt ttattacagg gacagcagag atccagtttg gttagtaccg ggccctacgc
     5701 gtgaggggcg cggaagccgg ggcggaggag gagccaaccc cgggctgggc tgagacccgc
     5761 agaggaagac gctctaggga tttgtcccgg actagcgaga tggcaaggct gaggacggga
     5821 ggctgattga gaggcgaagg tacaccctaa tctcaataca acctttggag ctaagccagc
     5881 aatggtagag ggaagattct gcacgtccct tccaggcggc ctccccgtca ccaccccccc
     5941 caacccgccc cgaccggagc tgagagtaat tcatacaaaa ggactcgccc ctgccttggg
     6001 gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc
     6061 cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc
     6121 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6181 ggtcggcaat tgaaccggtg cctagagaaa gtggcgcggg gtaaactggg aaagtgatgt
     6241 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6301 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     6361 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     6421 cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     6481 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     6541 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     6601 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     6661 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     6721 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     6781 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     6841 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     6901 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     6961 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     7021 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     7081 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     7141 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     7201 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     7261 tggttcaaag tttttttctt ccatttcagg tgtcgtggaa gcttggcgcg cccgtacggc
     7321 caccatggct tcaaacttta ctcagttcgt gctcgtggac aatggtggga caggggatgt
     7381 gacagtggct ccttctaatt tcgctaatgg ggtggcagag tggatcagct ccaactcacg
     7441 gagccaggcc tacaaggtga catgcagcgt caggcagtct agtgcccaga agagaaagta
     7501 taccatcaag gtggaggtcc ccaaagtggc tacccagaca gtgggcggag tcgaactgcc
     7561 tgtcgccgct tggaggtcct acctgaacat ggagctcact atcccaattt tcgctaccaa
     7621 ttctgactgt gaactcatcg tgaaggcaat gcaggggctc ctcaaagacg gtaatcctat
     7681 cccttccgcc atcgccgcta actcaggtat ctacagcgct ggaggaggtg gaagcggagg
     7741 aggaggaagc ggaggaggag gtagcggacc taagaaaaag aggaaggtgg cggccgctgg
     7801 atccccttca gggcagatca gcaaccaggc cctggctctg gcccctagct ccgctccagt
     7861 gctggcccag actatggtgc cctctagtgc tatggtgcct ctggcccagc cacctgctcc
     7921 agcccctgtg ctgaccccag gaccacccca gtcactgagc gctccagtgc ccaagtctac
     7981 acaggccggc gaggggactc tgagtgaagc tctgctgcac ctgcagttcg acgctgatga
     8041 ggacctggga gctctgctgg ggaacagcac cgatcccgga gtgttcacag atctggcctc
     8101 cgtggacaac tctgagtttc agcagctgct gaatcagggc gtgtccatgt ctcatagtac
     8161 agccgaacca atgctgatgg agtaccccga agccattacc cggctggtga ccggcagcca
     8221 gcggcccccc gaccccgctc caactcccct gggaaccagc ggcctgccta atgggctgtc
     8281 cggagatgaa gacttctcaa gcatcgctga tatggacttt agtgccctgc tgtcacagat
     8341 ttcctctagt gggcagggag gaggtggaag cggcttcagc gtggacacca gtgccctgct
     8401 ggacctgttc agcccctcgg tgaccgtgcc cgacatgagc ctgcctgacc ttgacagcag
     8461 cctggccagt atccaagagc tcctgtctcc ccaggagccc cccaggcctc ccgaggcaga
     8521 gaacagcagc ccggattcag ggaagcagct ggtgcactac acagcgcagc cgctgttcct
     8581 gctggacccc ggctccgtgg acaccgggag caacgacctg ccggtgctgt ttgagctggg
     8641 agagggctcc tacttctccg aaggggacgg cttcgccgag gaccccacca tctccctgct
     8701 gacaggctcg gagcctccca aagccaagga ccccactgtc tcctgtacag gcagtggaga
     8761 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc caaccatgaa
     8821 aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     8881 ctccgacctg atgcagctct ccgagggcga agaatctcgg gctttcagct tcgatgtggg
     8941 agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat ggtttctaca aagatcgcta
     9001 tgtttatcgg cactttgcat ccgccgctct ccctattccc gaagtgcttg acattgggga
     9061 gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca ccttgcaaga
     9121 cctgcctgaa accgaactgc ccgctgttct ccagcccgtc gccgaggcca tggatgccat
     9181 cgctgccgcc gatcttagcc agaccagcgg gttcggccca ttcggacctc aaggaatcgg
     9241 tcaatacact acatggcgcg atttcatctg cgctattgct gatccccatg tgtatcactg
     9301 gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc caggctctcg atgagctgat
     9361 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgccgatt tcggctccaa
     9421 caatgtcctg accgacaatg gccgcataac agccgtcatt gactggagcg aggccatgtt
     9481 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccctggt tggcttgtat
     9541 ggagcagcag acccgctact tcgagcggag gcatcccgag cttgcaggat ctcctcggct
     9601 ccgggcttat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     9661 tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca atcgtccggt ccggagccgg
     9721 gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc gtctggaccg atggctgtgt
     9781 ggaagtgctc gccgatagtg gaaacagacg ccccagcact cgtcctaggg caaaggatct
     9841 gcagtaatga gaattcgcgg ccgctcaacc tctggattac aaaatttgtg aaagattgac
     9901 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     9961 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    10021 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    10081 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    10141 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    10201 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    10261 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    10321 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
    10381 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
    10441 cgcctccccg caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
//
