LOCUS       sequence-217741-        9482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of png genomic DNA into 
            Drosophila genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Fenger DD, Carminati JL, Burney-Sigman DL, Kashevsky H, Dines JL, 
            Elfring LK, Orr-Weaver TL
  TITLE     PAN GU: a protein kinase that inhibits S phase and promotes mitosis 
            in early Drosophila development.
  JOURNAL   Development. 2000 Nov;127(22):4763-74.
  PUBMED    11044392
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2000-11"; volume: "127(22)"; pages: "4763-74"
COMMENT     Sequence Label: C2 png genomic in Casper
FEATURES             Location/Qualifiers
     source          1..9482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(824..854,855..1409)
                     /label=P element 5' end
     CDS             complement(join(2188..2802,2873..3004,3208..3523,3585..4239,
                     4314..4587,4989..5060))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5512..5530
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     5561..5577
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5562..5578
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(7312..7513,7514..7544)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(8082..8101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8201..8223
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8261..8279)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8347..8451
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8452..8520,8521..9312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8670..8689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccttctt
      601 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
      661 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
      721 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
      781 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gcccatgatg aaataacata
      841 aggtggtccc gtcgatagcc gaagcttacc gaagtataca cttaaattca gtgcacgttt
      901 gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat taacccttac
      961 gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca aagctgtgac tggagtaaaa
     1021 ttaattcacg tgccgaagtg tgctattaag agaaaattgt gggagcagag ccttgggtgc
     1081 agccttggtg aaaactccca aatttgtgat acccacttta atgattcgca gtggaaggct
     1141 gcacctgcaa aaggtcagac atttaaaagg aggcgactca acgcagatgc cgtacctagt
     1201 aaagtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg gagtacacaa
     1261 acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt
     1321 taggtcctgt tcattgttta atgaaaataa gagcttgagg gaaaaaattc gtactttgga
     1381 gtacgaaatg cgtcgtttag agcagcagcc gaattaattc tagttccagt gaaatccaag
     1441 cattttctaa attaaatgta ttcttattat tatagttgtt atttttgata tatataaaca
     1501 acactattat gcccaccatt tttttgagat gcatctacac aaggaacaaa cactggatgt
     1561 cactttcagt tcaaattgta acgctaatca ctccgaacag gtcacaaaaa attaccttaa
     1621 aaagtcataa tattaaatta gaataaatat agctgtgagg gaaatatata caaatatatt
     1681 ggagcaaata aattgtacat acaaatattt attactaatt tctattgaga cgaaatgaac
     1741 cactcggaac catttgagcg aaccgaatcg cgcggaacta acgacagtcg ctccaaggtc
     1801 gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa gagcaactac
     1861 gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa agtatgtata
     1921 taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt aaggtatgca
     1981 ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg atgttcgaag
     2041 gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat ataattacat
     2101 tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa
     2161 caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc
     2221 cagatatgcg agcacccgga agctcacgat gagaatggcc agacccacgt agtccagcgg
     2281 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     2341 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     2401 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     2461 caccgagccc gagttcaaga agaagccgcc aaagagcagg aatggtatga taaccggcgg
     2521 acccacagac agcgccatcg aggtcgagga gctggcgcag gatattagat atccgaagga
     2581 cgttgacaca ttggccacca gagtgaccag cgccaggcag ttgaagaagt gcagcactcc
     2641 ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag accagtggca ctgtgagaaa
     2701 aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat aaagtcgact
     2761 tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg aatcgattca
     2821 ttagaaagtt agtaaattat tgaaatgcaa atgtattcta aacatgactt acatttatcg
     2881 tggcaaagac gttttgaaag gtcatgttgg tcaggaagag gaagatggct ccgttgatat
     2941 tcatcacacc cacttgcgtg agttgttggc ccaaaaagat gaggccaatc aagatggcaa
     3001 ccatctgcaa attaaaatgt tactcgcatc tcattaatat tcgcgagtta aatgaaattt
     3061 atttatcttc tgcaaaacta taaactatac atctcattga aaaaaactaa gaagggtgtg
     3121 gaatcaggca attctatcta aaatctagcg aatttgtttc caagaattgt aagcgttata
     3181 tcatttgttt ccactggaac cactcaccgt tgtctgaata agtcgcactt ttacgaggag
     3241 tggttccttg agcaccgaca gccaggatcg ccacaggacc gcccggaact gcatgaacca
     3301 ggtggccttg taggtgtacc cattctccgg ctgctccagt ggcttctcca gatttttggt
     3361 ggccaacaac tgctccatat cccgggctac tttgctaatg gcaaaattgt cgcatatctt
     3421 ggcgatccga tcacgggact cgatctcccg tccgggcaca acggccaaca cctgtacgta
     3481 aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc acgctggata ggagttgaga
     3541 tgttatgtaa tactagatac ccttaataaa cacatcgaac tcactaggaa aagaagtcga
     3601 cggcttcgct gggagtgccc aagaaagcta ccctgccctc ggccatcaga aggatcttgt
     3661 caaagagctc aaacagctcg gaagacggct gatgaatggt caggatgacg gtcttgccct
     3721 tctgcgacag cttcttcagc acctggacga cgctgtgggc ggtaaaggag tccagtccgg
     3781 aggtgggctc atcgcagatc agaagcggcg gatcggttag agcctcggag gcgaatgcca
     3841 gacgcttcct ttctccgccg gacagacctt tcaccctgcc gggcacaccg atgatcgtgt
     3901 gctgacattt gctgagcgaa agctcctgga tcacctgatc cacgcgggcc actcgctgcc
     3961 gataggtcag atgtcgtggc atccgcacca tggcttggaa aatcaggtgt tccctggccg
     4021 ttagggagcc gataaagagg tcatcctgct ggacataggc gcacctggcc tgcatctcct
     4081 tggcgtccac aggttggcca ttgagcagtc gcatcccgga tggcgatact tggatgccct
     4141 gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt tccggcaccg gaactgccca
     4201 tcacggccaa aagttcgccc ggataggcca cgccgcaaac tgagtttcaa attggtaatt
     4261 ggacccttta ttaagatttc acacagatca gccgactgcg aatagaaact caccgttctt
     4321 gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg cagaatagtc cgcgtgtccg
     4381 gttgaccagc tgccgccatc cggagcccgg ctgattgacc gccccaaaga tgtccatatt
     4441 gtgccaggca taggtgaggt tctcggctag ttggccgctc cctgaaccgg agtcctccgg
     4501 cggactgggt ggccggagcg tgccgtagtt tttggcctgc ccgaagccct ggttaatgca
     4561 gctctgcgaa gccgctccgc tgtcaccctg caatgatagg ggatctcaaa tatcaactac
     4621 aagcgttatg ctcatctaac cccgaacaaa aagtaccccg aagtatccta cgaagtaggt
     4681 ttatactttt atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat
     4741 tttttgtaaa aaagaatata gtcgaaaatg aatgccttta gatgtcttga tcatgatatg
     4801 atctcaaaaa ttgtcttata tagcgagaac agctaccaga ataatctgtt tcgtgtcact
     4861 atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca
     4921 atttgatgtt gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta
     4981 ctactcacat tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc
     5041 tcctgatcct cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa
     5101 aaccaatcac caccccaatc actcaaaaaa caaacaaaaa taagaagcga gaggagtttt
     5161 ggcacagcac tttgtgttta attgatggcg taaaccgctt ggagcttcgt cacgaaaccg
     5221 ctgacaaaat gcaactgaag gcggacattg acgctacgta acgctacaaa cggtggcgaa
     5281 agagatagcg gacgcagcgg cgaaagagac ggcgatattt ctgtggacag agaaggaggc
     5341 aaacagcgct gactttgagt ggaatgtcat tttgagtgag aggtaatcga aagaacctgg
     5401 tacatcaaat acccttggat cgaagtaaat ttaaaactga tcagataagt tcaatgatat
     5461 ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt tccgcaaaaa tgggttttat
     5521 taacttacat acatactaga attcggtacc gggccccccc tcgaggtcga cggtatcgat
     5581 aagctttaga ggattaatgt tactgtacaa taggtgaaca aatgtaaatt aaagactacg
     5641 ttaatgacat tgagcgagac tgttttggaa tatacattat atactgttta tagctgacag
     5701 atcccctggc aaaagcacag gcgcacacaa agcacagcac acaccttaat ttatcgctta
     5761 agctagccta gttcttaagt acaatgaaat atttgttgag gtctcctcgt gctggtgagc
     5821 tgaccattgg cttccgttag tagctgtagc tgaagtaatg cttgtagagg gtcacagtca
     5881 tgccggcgtc gaggtggaag atgtctggta ggcagatgcg gcgctcctgc tcgtaaacga
     5941 ccatcagctc gcaaacttgc gcccacacgg gatcgtagag acgccggatg agctggcagt
     6001 cgagccgggg gcggaccagc gcctggacta cggtgtgcag tagggcaggg gtggcgttct
     6061 tgtcgagaaa cgcggcgaag gggctgcgtc cgaggcacag ctcgtagagg actagcccca
     6121 gtgaccacac gtccgacttg aaatccacct tgccctggct ggacatcgcc tccggggcca
     6181 tgtacatggg cgtgcccatg cccgcctgca gttgagtgct gggcgcatga acgttcgcga
     6241 tcccaaagtc ggcgatcttg acgcggtcgt tggcgtcgag gagcatgttc tcgggcttga
     6301 tgtctcggtg gatgacgcag cggatgtgca ggtactcgag tcccactacc atgtcgcgga
     6361 agtagcccat cagccgctcc tgattgactc ccccggtgcc tgacggcagc tgctggatga
     6421 catccctcaa ggtgccgttg ggcacgtact ccatcacgat gttcacagtg cccgcgtggc
     6481 taaaggagcg caggaactca acgatgtgcg ggtggcgcag ctgcgagatg atgtacacct
     6541 cctccttgat caatcccaac tctgtcttcg gattccggac gatgatccgc ttcacgcaca
     6601 ccttcctctc cggctgtccg cgcacctcgt tttggtggca gaggaagacc cggccaaagg
     6661 tgccctggcc cagcaccctt accgcacgca gcttagagtc acccagttcc atctcgagtg
     6721 cgcagagttc cgaaagcgta atccgcgatc ccgccttgat gtacagttag ccagcaaaag
     6781 cgcaagcgcg ccatctatcg attgcttcga aacaagagat gcacagttcc atcggaaaaa
     6841 gccgaaaact cggaacttcg gaaaccccat atgaaaattg ctcatctggc cagcagcaac
     6901 agtgctcaat gaaagtaagt tattagagtc tggttaatta atcaaactaa cgcatcgtga
     6961 actaacccca ttcaaaaagt ccatccatgt gaaaagtatg actatcctaa atttttattt
     7021 attttattta acaaaattaa atatagtata caatactaaa cctaattaaa tttaatgagt
     7081 actagctacg ggggccttcg actcgagact tatgggttaa ttgctttata taataaactt
     7141 agctttttgg acaaatttgt aaatttttga gatattttca caggatggga tacttaaaca
     7201 attcgagaga ctatgtgtac caaataagtg agatcctcta gagtcgacct cgaacgttaa
     7261 cgttaacgta acgttaactc gaggcctcga ggtcgacctg cagccaagct ttgcgtactc
     7321 gcaaattatt aaaaataaaa ctttaaaaat aatttcgtct aattaatatt atgagttaat
     7381 tcaaacccca cggacatgct aagggttaat caacaatcat atcgctgtct cactcagact
     7441 caatacgaca ctcagaatac tattcctttc actcgcactt attgcaagca tacgttaagt
     7501 ggatgtctct tgccgacggg accaccttat gttatttcat catggtctgg ccattctcat
     7561 cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta
     7621 gccgacatat atccgaaata actgcttgtt tttttttttt accattatta ccatcgtgtt
     7681 tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga
     7741 tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt
     7801 tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc
     7861 tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc
     7921 ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc
     7981 tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg
     8041 attcggtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     8101 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8161 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8221 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     8281 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8341 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8401 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8461 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8521 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8581 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8641 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8701 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8761 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8821 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8881 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8941 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9001 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9061 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9121 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9181 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9301 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9361 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9421 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9481 tc
//
