LOCUS       sequence-217756-        7856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  snu114-del(IV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314 snu114-del(IV)
FEATURES             Location/Qualifiers
     source          1..7856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    104..125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(140..145,146..163,164..170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    178..194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     183..205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     202..218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     202..218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             610..699
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(3453..3472)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3454..3472)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3479..3496)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3479..3495)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3488..3510)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3636..4091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3723..3742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3933..3954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4194..4868)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(4869..5149)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5170..5189)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5289..5311
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5349..5367)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5408..5911
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        5948..6052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6053..6121,6122..6913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6271..6290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7084..7672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7573..7592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7826..7843
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
       61 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa
      241 ttaaccctca ctaaagggaa caaaagctgg taccgggccc cccctcgagg tagcgtatac
      301 tctggcattt ttcatttatt tgacttattt ttatttattt tattgtacaa tggcaaacaa
      361 atacacggac tggtatgttc ttcgtcaaat tgatgatctg ttcttttggc ttatccttat
      421 gggatgatta atgattgtta aagcctcatt gcggaaattt taatgccgga aaggcctgaa
      481 aaatttcaaa gtggcagaaa acaaggcttt ggggcattac ttgccaacgg ctgacgattg
      541 ctagctgctt ttacagaagc gatacacaat agctcctaac atagcaaaat ggaattcggc
      601 cgcatctttt acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
      661 tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag atctgaaggt
      721 gacgatttat tcgatgagtt tggaaatttg atcggagttg atccttttga ctcggatgaa
      781 gaagaaagtg tgctggatga gcaagaacaa tatcaaacca atacgtttga ggggagcggc
      841 aacaacaatg agattgagag cagacaactt acctcgttag gaagtaaaaa ggaacttgga
      901 atttccttag agcaccctta tggtaaggaa gtggaagtat taatggaaac taagaacaca
      961 cagtcaccac agactccact ggtagagccg gttacagaac gaacaaagtt gcaagagcat
     1021 acaattttta cacaattaaa aaaaaacatt ccgaagacta gatataatcg agactatatg
     1081 ttatcaatgg ctaacattcc ggagaggata ataaatgttg gtgtcattgg acctcttcac
     1141 tcaggtaaga cctctttgat ggatctccta gtaatcgact cacacaaacg cataccagat
     1201 atgtctaaga atgtagaact tggatggaaa ccgctaagat acctggataa tttgaaacaa
     1261 gagattgacc ggggtctttc cattaaactt aatggttcca ctctactctg caccgattta
     1321 gaatccaagt ctagaatgat aaactttttg gatgcaccag ggcatgttaa ttttatggat
     1381 gaaacggcag tcgcacttgc agcaagtgat ctggttttaa tcgtgataga tgttgtagaa
     1441 ggtgttacat ttgtggtaga gcaattgata aaacaaagta ttaaaaataa tgtagcaatg
     1501 tgttttgtta taaataaact cgacagattg attctagatt taaagttgcc accaatggac
     1561 gcatacttga agttaaatca tataattgca aatatcaatt cctttacgaa gggaaacgtg
     1621 ttttcaccaa tagacaataa cataatattt gcgtctacaa agctgggctt cacattcact
     1681 atcaaggaat ttgtttctta ttattacgcc cactccatac cttcttctaa gatagacgac
     1741 tttaccacaa ggctatgggg tagcgtatat taccataaag gtaatttcag gacaaaacca
     1801 tttgaaaatg tagagaagta cccgactttc gttgaattta tcctcatccc gctttataaa
     1861 atattttcct acgctttatc tatggagaaa gataaattaa agaatttgtt aaggtctaac
     1921 tttagagtta atttaagtca agaggccctc cagtacgatc cgcaaccttt tttaaaacac
     1981 gttctacagt taatatttag gcaacaaacc ggtcttgtag atgcgataac cagatgttat
     2041 caaccatttg aattattcga caataagaca gctcatcttt caattccagg aaaatctaca
     2101 ccagaaggca ctctttgggc tcatgtactg aaaactgtgg attacggtgg agcagagtgg
     2161 tcccttgtac ggatatactc tggacttttg aagagagggg atacagtacg tatacttgat
     2221 acatcacaat ctgaatctcg ccaaaaaaga cagttgcatg atatctcaaa gacggaaacc
     2281 tccaacgaag atgaagatga agatgacgaa acgccttcat gtgaggtcga agaaattggc
     2341 ttattaggtg gcagatatgt ctatccggtt catgaagcac acaaaggaca gatagtatta
     2401 ataaaaggta tttcaagtgc atatattaaa tccgcgacgt tgtactctgt aaagagtaag
     2461 gaggacatga aacagctaaa atttttcaaa cctttagatt atattaccga ggcagttttc
     2521 aaaattgttc ttcaaccttt actgccaagg gaattaccca agttattgga tgccctgaac
     2581 aaaatttcga aatactaccc aggcgttatt attaaagttg aggaatctgg agaacatgta
     2641 atactaggaa atggagagct gtatatggac tgtttgttgt atgatctaag agcgagttat
     2701 gcaaagatag aaattaaaat atctgatcct ttaactgttg cggccgcagc aatcccaatt
     2761 ttattggaac ccatctatga agttgacatc acggtccacg cccccttgct gccaatagta
     2821 gaggaactta tgaagaagag acgtggaagc aggatataca aaacaataaa agtggcaggg
     2881 acaccattgt tggaggttcg tggacaagtt ccggttattg aatctgcagg attcgagaca
     2941 gatttgagat tatctacgaa tggtcttggt atgtgtcagc tgtacttttg gcacaagata
     3001 tggaggaagg ttcctggtga tgttttggat aaagatgcgt ttattccaaa attgaaaccc
     3061 gcacctatca acagtttaag tcgtgatttc gtgatgaaaa caagaaggcg gaagggtatt
     3121 tctacaggtg gatttatgtc aaatgatggt cctacgcttg aaaagtatat aagcgctgaa
     3181 ttatacgctc aattaagaga aaatggctta gtaccgtgaa aatcttggcg cataagaatt
     3241 aagcaatatg tccacaatat ttttattcag cagaatttac atatgacttc gtatttagtg
     3301 atataactac tcatcgggac tgaaaatttt ttatttctgc ctaaatacag atgagatgag
     3361 ctttatagaa ttttacgctg ttaaaaagtt aaacggtaac aaagcacagt tagttgaaca
     3421 acggtatata gagaatggcg agctccaatt cgccctatag tgagtcgtat tacaattcac
     3481 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3541 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3601 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     3661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3781 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3841 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3901 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3961 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4021 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4081 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4141 atagatcggc aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc
     4201 ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca
     4261 cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct
     4321 ggcgtcagtc caccagctaa cataaaatgt aagctttcgg ggctctcttg ccttccaacc
     4381 cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac
     4441 aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt
     4501 ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac
     4561 tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc caaaacatcc
     4621 tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga actattttta
     4681 tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt tctctttcta
     4741 ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca ttctgcggcc
     4801 tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt gaaattaata
     4861 acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt
     4921 caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt aattcttaat
     4981 cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc tatttttcaa
     5041 taaagaatat cttccactac tgccatctgg cgtcataact gcaaagtaca catatattac
     5101 gatgctgttc tattaaatgc ttcctatatt atatatatag taatgtcgtg atctatggtg
     5161 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5221 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5281 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5341 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5401 ttagacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg atggaataat
     5461 ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt tagaaagtaa
     5521 ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat
     5581 ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga
     5641 tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc
     5701 tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga gcaagataaa
     5761 aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat
     5821 tttttcttta atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat
     5881 ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg
     5941 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6001 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6061 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagc
//
