LOCUS       sequence-217760-       14289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV driven mammalian expression of UBR5 with an N-termianl GFP 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14289)
  AUTHORS   Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, 
            Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B,
            Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C
  TITLE     TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at 
            damaged chromosomes.
  JOURNAL   Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. 
            Epub 2012 Aug 9.
  PUBMED    22884692
REFERENCE   2  (bases 1 to 14289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.06.039"; journalName: "Cell"; date: 
            "2012-08-17- 17"; volume: "150"; issue: "4"; pages: "697-709"
COMMENT     Sequence Label: GFP-UBR5
FEATURES             Location/Qualifiers
     source          1..14289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(709..711,712..714,715..1425)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(754..775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1015..1034)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1362..1383
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1441..1465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9880..9904)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9912..9953
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(9993..10012)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10037..10085
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10287..10715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10374..10393)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10584..10605
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10724..10744)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10729..11058
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10909..11044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10971..10990
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11106..11153
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11172..11570
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11728..11849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11765..11784)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11819..11838
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11898..11914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11898..11914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11911..11933)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11922..11938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11946..11952,11953..11970,11971..11976))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11991..12012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12129..12146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12300..12888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12380..12399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13059..13850,13851..13919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13682..13701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13920..14024)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14099..14118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatccctacc ggtgatatcc tcgagaccat ggtgagcaag
      721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      901 ttcacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1141 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
     1201 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1321 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggctc gagcccatca
     1441 acaagtttgt acaaaaaagc aggcttccgg accatgacgt ccatccattt cgtggttcac
     1501 ccgctgccgg gcaccgagga ccagctcaat gacaggttac gagaagtttc tgagaagctg
     1561 aacaaatata atttaaacag ccacccccct ttgaatgtat tggaacaggc tactattaaa
     1621 cagtgtgtgg tgggaccaaa tcatgctgcc tttcttcttg aggatggtag agtttgcagg
     1681 attggttttt cagtacagcc agacagattg gaattgggta aacctgataa taatgatggg
     1741 tcaaagttga acagcaactc gggggcaggg aggacgtcaa ggcctggtag gacaagcgac
     1801 tctccatggt ttctctcagg ttctgagact ctaggcaggc tggcaggcaa caccttagga
     1861 agccgctgga gttctggagt gggtggaagt ggtggaggat cctctggtag gtcatcagct
     1921 ggagctcgag attcccgccg gcagactcga gttattcgga caggacggga tcgagggtct
     1981 gggcttttgg gcagtcagcc ccagccagtt attccagcat ctgtcattcc agaggagctg
     2041 atttcacagg cccaagttgt tttacaaggc aaatccagaa gtgtcattat tcgagaactt
     2101 cagagaacaa atcttgatgt gaaccttgct gtaaataatt tacttagccg ggatgatgaa
     2161 gatggagatg atggggatga tacagccagc gaatcttatt tgcctggaga ggatcttatg
     2221 tctctccttg atgccgacat tcattctgcc cacccaagtg tcattattga tgcagatgcc
     2281 atgttttctg aagacattag ctattttggt tacccttctt ttcgtcgttc atcactttcc
     2341 aggctaggct catctcgagt tctccttctt cccttagaga gagactctga gctgttgcgt
     2401 gaacgtgaat ccgttttacg tttacgtgaa cgaaggtggc ttgatggagc ctcatttgat
     2461 aatgaaaggg gttctaccag caaggaagga gagccaaact tggataagaa gaatacacct
     2521 gttcaaagtc cagtatctct aggagaagat ttgcagtggt ggcctgataa ggatggaaca
     2581 aaattcatct gtattggggc tctgtattct gaacttctgg ctgtcagcag taaaggagaa
     2641 ctttatcagt ggaaatggag tgaatctgag ccttacagaa atgcccagaa tccttcatta
     2701 catcatccac gagcaacatt tttggggtta accaatgaaa agatagtcct cctgtctgca
     2761 aatagcataa gagcaactgt agctacagaa aataacaagg ttgctacatg ggtggatgaa
     2821 actttaagtt ctgtggcttc taaattagag cacactgctc agacttactc tgaacttcaa
     2881 ggagagcgga tagtttcttt acattgctgt gccctttaca cctgcgctca gctggaaaac
     2941 agtttatatt ggtggggtgt agttcctttt agtcaaagga ggaaaatgtt agagaaagct
     3001 agagcaaaaa ataaaaagcc taaatccagt gctggtattt cttcaatgcc gaacatcact
     3061 gttggtaccc aggtatgctt gagaaataat cctctttatc atgctggagc agttgcattt
     3121 tcaattagtg ctgggattcc taaagttggt gtcttaatgg agtcagtttg gaatatgaat
     3181 gacagctgta gatttcaact tagatctcct gaaagcttga aaaacatgga aaaagctagc
     3241 aaaactactg aagctaagcc tgaaagtaag caggagccag tgaaaacaga aatgggtcct
     3301 ccaccatctc cagcatccac gtgtagtgat gcatcctcaa ttgccagcag tgcatcaatg
     3361 ccatacaaac gacgacggtc aacccctgca ccaaaagaag aggaaaaggt gaatgaagag
     3421 cagtggtctc ttcgggaagt ggtttttgtg gaagatgtca agaatgttcc tgttggcaag
     3481 gtgctaaaag tagatggtgc ctatgttgct gtaaaatttc caggaacctc cagtaatact
     3541 aactgtcaga acagctctgg tccagatgct gacccttctt ctctcctgca ggattgtagg
     3601 ttacttagaa ttgatgaatt gcaggttgtc aaaactggtg gaacaccgaa ggttcccgac
     3661 tgtttccaaa ggactcctaa aaagctttgt atacctgaaa aaacagaaat attagcagtg
     3721 aatgtagatt ccaaaggtgt tcatgctgtt ctgaagactg gaaattgggt acgatactgt
     3781 atctttgatc ttgctacagg aaaagcagaa caggaaaata attttcctac aagcagcatt
     3841 gctttccttg gtcagaatga gaggaatgta gccattttca ctgctggaca ggaatctccc
     3901 attattcttc gagatggaaa tggtaccatc tacccaatgg ccaaagattg catgggagga
     3961 ataagggatc ccgattggct ggatcttcca cctattagta gtcttggaat gggtgtgcat
     4021 tctttaataa atcttcctgc caattcaaca atcaaaaaga aagctgctgt tatcatcatg
     4081 gctgtagaga aacaaacctt aatgcaacac attctgcgct gtgactatga ggcctgtcga
     4141 caatatctaa tgaatcttga gcaagcggtt gttttagagc agaatctaca gatgctgcag
     4201 acattcatca gccacagatg tgatggaaat cgaaatattt tgcatgcttg tgtatcagtt
     4261 tgctttccaa ccagcaataa agaaactaaa gaagaagagg aagcggagcg ttctgaaaga
     4321 aatacatttg cagaaaggct ttctgctgtt gaggccattg caaatgcaat atcagttgtt
     4381 tcaagtaatg gcccaggtaa tcgggctgga tcatcaagta gccgaagttt gagattacgg
     4441 gaaatgatga gacgttcgtt gagagcagct ggtttgggta gacatgaagc tggagcttca
     4501 tccagtgacc accaggatcc agtttcaccc cccatagctc cccctagttg ggttcctgac
     4561 cctcctgcga tggatcctga tggtgacatt gattttatcc tggcccccgc tgtgggatct
     4621 cttaccacag cagcaaccgg tactggtcaa ggaccaagca cctccactat tccaggtcct
     4681 tccacagagc catctgtagt agaatccaag gatcgaaagg cgaatgctca ttttatattg
     4741 aaattgttat gtgacagtgt ggttctccag ccctatctac gagaacttct ttctgccaag
     4801 gatgcaagag ggatgacccc atttatgtca gctgtaagtg gccgagctta tcctgctgca
     4861 attaccatct tagaaactgc tcagaaaatt gcaaaagctg aaatatcctc aagtgaaaaa
     4921 gaggaagatg tattcatggg aatggtttgc ccatcaggta ccaaccctga tgactctcct
     4981 ttatatgttt tatgttgtaa tgacacttgc agttttacat ggactggagc agagcacatt
     5041 aaccaggata tttttgagtg tcgaacttgt ggcttgctgg agtcactgtg ttgttgtacg
     5101 gaatgtgcaa gggtttgtca taaaggtcat gattgcaaac tcaaacggac atcaccaaca
     5161 gcctactgtg attgttggga gaaatgtaaa tgtaaaactc ttattgctgg acagaaatct
     5221 gctcgtcttg atctacttta tcgcctgctc actgctacta atctggttac tctgccaaac
     5281 agcaggggag agcacctctt actattctta gtacagacag tcgcaaggca gacggtggag
     5341 cattgtcaat acaggccacc tcgaatcagg gaagatcgta accgaaaaac agccagtcct
     5401 gaagattcag atatgccaga tcatgatttg gagcctccaa gatttgccca gcttgcattg
     5461 gagcgtgttc tacaggactg gaatgccttg aaatctatga ttatgtttgg gtcgcaggag
     5521 aataaagacc ctcttagtgc cagcagtaga ataggccatc ttttgccaga agagcaagta
     5581 tacctcaatc agcaaagtgg cacaattcgg ctggactgtt tcactcattg ccttatagtt
     5641 aagtgtacag cagatatttt gcttttagat actctactag gtacactagt gaaagaactc
     5701 caaaacaaat atacacctgg acgtagagaa gaagctattg ctgtgacaat gaggtttcta
     5761 cgttcagtgg caagagtttt tgttattctg agtgtggaaa tggcttcatc caaaaagaaa
     5821 aacaacttta ttccacagcc aattggaaaa tgcaagcgtg tattccaagc attgctacct
     5881 tacgctgtgg aagaattgtg caacgtagca gagtcactga ttgttcctgt cagaatgggg
     5941 attgctcgtc caactgcacc atttaccctg gctagtacta gcatagatgc catgcagggc
     6001 agtgaagaat tattttcagt ggaaccacta ccaccacgac catcatctga tcagtctagc
     6061 agctccagtc agtctcagtc atcctacatc atcaggaatc cacagcagag gcgcatcagc
     6121 cagtcacagc ccgttcgggg cagagatgaa gaacaggatg atattgtttc agcagatgtg
     6181 gaagaggttg aggtggtgga gggtgtggct ggagaagagg atcatcatga tgaacaggaa
     6241 gaacacgggg aagaaaatgc tgaggcagag ggacaacatg atgagcatga tgaagacggg
     6301 agtgatatgg agctggactt gttagcagca gctgaaacag aaagtgatag tgaaagtaac
     6361 cacagcaacc aagataatgc tagtgggcgc agaagcgttg tcactgcagc aactgctggt
     6421 tcagaagcag gagcaagcag tgttcctgcc ttcttttctg aagatgattc tcaatcgaat
     6481 gactcaagtg attctgatag cagtagtagt cagagtgacg acatagaaca ggagaccttt
     6541 atgcttgatg agccattaga aagaaccaca aatagctccc atgccaatgg tgctgcccaa
     6601 gctccccgtt caatgcagtg ggctgtccgc aacacccagc atcagcgagc agccagtaca
     6661 gccccttcca gtacatctac accagcagca agttcagcgg gtttgattta tattgatcct
     6721 tcaaacttac gccggagtgg taccatcagt acaagtgctg cagctgcagc agctgctttg
     6781 gaagctagca acgccagcag ttacctaaca tctgcaagca gtttagccag ggcttacagc
     6841 attgtcatta gacaaatctc ggacttgatg ggccttattc ctaagtataa tcacctagta
     6901 tactctcaga ttccagcagc tgtgaaattg acttaccaag atgcagtaaa cttacagaac
     6961 tatgtagaag aaaagcttat tcccacttgg aactggatgg tcagtattat ggattctact
     7021 gaagctcaat tacgttatgg ttctgcatta gcatctgctg gtgatcctgg acatccaaat
     7081 catcctcttc acgcttctca gaattcagcg agaagagaga ggatgactgc gcgagaagaa
     7141 gctagcttac gaacacttga aggcagacga cgtgccacct tgcttagcgc ccgtcaagga
     7201 atgatgtctg cacgaggaga cttcctaaat tatgctctgt ctctaatgcg gtctcataat
     7261 gatgagcatt ctgatgttct tccagttttg gatgtttgct cattgaagca tgtggcatat
     7321 gtttttcaag cacttatata ctggattaag gcaatgaatc agcagacaac attggataca
     7381 cctcaactag aacgcaaaag gacgcgagaa ctcttggaac tgggtattga taatgaagat
     7441 tcagaacatg aaaatgatga tgacaccaat caaagtgcta ctttgaatga taaggatgat
     7501 gactctcttc ctgcagaaac tggccaaaac catccatttt tccgacgttc agactccatg
     7561 acattccttg ggtgtatacc cccaaatcca tttgaagtgc ctctggctga agccatcccc
     7621 ttggctgatc agccacatct gttgcagcca aatgctagaa aggaggatct ttttggccgt
     7681 ccaagtcagg gtctttattc ttcatctgcc agtagtggga aatgtttaat ggaggttaca
     7741 gtggatagaa actgcctaga ggttcttcca acaaaaatgt cttatgctgc caatctgaaa
     7801 aatgtaatga acatgcaaaa ccggcaaaaa aaagaagggg aagaacagcc cgtgctgcca
     7861 gaagaaactg agagttcaaa accagggcca tctgctcatg atcttgctgc acaattaaaa
     7921 agtagcttac tagcagaaat aggacttact gaaagtgaag ggccacctct cacatctttc
     7981 aggccacagt gtagctttat gggaatggtt atttcccatg atatgctgct aggacgttgg
     8041 cgcctttctt tagaactgtt cggcagggta ttcatggaag atgttggagc agaacctgga
     8101 tcaatcctaa ctgaattggg tggttttgag gtaaaagaat caaaattccg cagagaaatg
     8161 gaaaaactga gaaaccagca gtcaagagat ttgtcactag aggttgatcg ggatcgagat
     8221 cttctcattc agcagactat gaggcagctt aacaatcact ttggtcgaag atgtgctact
     8281 acaccaatgg ctgtacacag agtaaaagtc acatttaagg atgagccagg agagggcagt
     8341 ggtgtagcac gaagttttta tacagccatt gcacaagcat ttttatcaaa tgaaaaattg
     8401 ccaaatctag agtgtatcca aaatgccaac aaaggcaccc acacaagttt aatgcagaga
     8461 ttaaggaacc gaggagagag agaccgggaa agggagagag aaagggaaat gaggaggagt
     8521 agtggtttgc gagcaggttc tcggagggac cgggatagag actttagaag acagctttcc
     8581 atcgacacta ggccctttag accagcctct gaagggaatc ctagcgatga tcctgagcct
     8641 ttgccagcac atcggcaggc acttggagag aggctttatc ctcgtgtaca agcaatgcaa
     8701 ccagcatttg caagtaaaat cactggcatg ttgttggaat tatccccagc tcagctgctt
     8761 ctccttctag caagtgagga ttctctgaga gcaagagtgg atgaggccat ggaactcatt
     8821 attgcacatg gacgggaaaa tggagctgat agtatcctgg atcttggatt agtagactcc
     8881 tcagaaaagg tacagcagga aaaccgaaag cgccatggct ctagtcgaag tgtagtagat
     8941 atggatttag atgatacaga tgatggtgat gacaatgccc ctttgtttta ccaacctggg
     9001 aaaagaggat tttatactcc aaggcctggc aagaacacag aagcaaggtt gaattgtttc
     9061 agaaacattg gcaggattct tggactatgt ctgttacaga atgaactctg tcctatcaca
     9121 ttgaatagac atgtaattaa agtattgctt ggtagaaaag tcaattggca tgattttgct
     9181 ttttttgatc ctgtaatgta tgagagtttg cggcaactaa tcctcgcgtc tcagagttca
     9241 gatgctgatg ctgttttctc agcaatggat ttggcatttg caattgacct gtgtaaagaa
     9301 gaaggtggag gacaggttga actcattcct aatggtgtaa atataccagt cactccacag
     9361 aatgtatatg agtatgtgcg gaaatacgca gaacacagaa tgttggtagt tgcagaacag
     9421 cccttacatg caatgaggaa aggtctacta gatgtgcttc caaaaaattc attagaagat
     9481 ttaacggcag aagattttag gcttttggta aatggctgcg gtgaagtcaa tgtgcaaatg
     9541 ctgatcagtt ttacctcttt caatgatgaa tcaggagaaa atgctgagaa gcttctgcag
     9601 ttcaagcgtt ggttctggtc aatagtagag aagatgagca tgacagaacg acaagatctt
     9661 gtttactttt ggacatcaag cccatcactg ccagccagtg aagaaggatt ccagcctatg
     9721 ccctcaatca caataagacc accagatgac caacatcttc ctactgcaaa tacttgcatt
     9781 tctcgacttt acgtcccact ctattcctct aaacagattc tcaaacagaa attgttactc
     9841 gccattaaga ccaagaattt tggttttgtg tagccgcgga cccagctttc ttgtacaaag
     9901 tggttgataa cgggaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     9961 gttagtaatg agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
    10021 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
    10081 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
    10141 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
    10201 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
    10261 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
    10321 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    10381 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10441 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10501 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10561 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10621 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10681 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
    10741 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    10801 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10861 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10921 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10981 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    11041 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
    11101 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
    11161 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
    11221 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
    11281 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
    11341 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
    11401 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
    11461 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
    11521 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg
    11581 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
    11641 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    11701 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    11761 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    11821 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    11881 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    11941 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    12001 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    12061 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    12121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    12181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    12241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    12301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    12361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    12421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    12481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    12541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    12601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    12661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    12721 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    12781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    12841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    12901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    12961 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    13021 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    13081 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    13141 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    13201 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    13261 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    13321 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    13381 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    13441 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    13501 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    13561 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    13621 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    13681 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    13741 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    13801 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    13861 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    13921 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    13981 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    14041 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
    14101 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
    14161 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
    14221 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
    14281 atacgcgtt
//
