LOCUS       sequence-217762-       11441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of gnu-GFP 9A into 
            Drosophila genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Hara M, Petrova B, Orr-Weaver TL
  TITLE     Control of PNG kinase, a key regulator of mRNA translation, is 
            coupled to meiosis completion at egg activation.
  JOURNAL   Elife. 2017 May 30;6. doi: 10.7554/eLife.22219.
  PUBMED    28555567
REFERENCE   2  (bases 1 to 11441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-05-30"; volume: "6. doi"; pages: " 10.7554/eLife.22219"
COMMENT     Sequence Label: gnu-GFP 9A
FEATURES             Location/Qualifiers
     source          1..11441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(824..854,855..1409)
                     /label=P element 5' end
     CDS             complement(join(2188..2802,2873..3004,3208..3523,3585..4239,
                     4314..4587,4989..5060))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5512..5530
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             complement(join(5846..6559,6560..6562,6563..6565))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5849..6559,6560..6562,6563..6565))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5891..5912)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6240..6259
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6499..6520
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6562..6571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(9271..9472,9473..9503)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(10041..10060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10160..10182
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10220..10238)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10306..10410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10411..10479,10480..11271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10629..10648)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccttctt
      601 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
      661 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
      721 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
      781 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gcccatgatg aaataacata
      841 aggtggtccc gtcgatagcc gaagcttacc gaagtataca cttaaattca gtgcacgttt
      901 gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat taacccttac
      961 gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca aagctgtgac tggagtaaaa
     1021 ttaattcacg tgccgaagtg tgctattaag agaaaattgt gggagcagag ccttgggtgc
     1081 agccttggtg aaaactccca aatttgtgat acccacttta atgattcgca gtggaaggct
     1141 gcacctgcaa aaggtcagac atttaaaagg aggcgactca acgcagatgc cgtacctagt
     1201 aaagtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg gagtacacaa
     1261 acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt
     1321 taggtcctgt tcattgttta atgaaaataa gagcttgagg gaaaaaattc gtactttgga
     1381 gtacgaaatg cgtcgtttag agcagcagcc gaattaattc tagttccagt gaaatccaag
     1441 cattttctaa attaaatgta ttcttattat tatagttgtt atttttgata tatataaaca
     1501 acactattat gcccaccatt tttttgagat gcatctacac aaggaacaaa cactggatgt
     1561 cactttcagt tcaaattgta acgctaatca ctccgaacag gtcacaaaaa attaccttaa
     1621 aaagtcataa tattaaatta gaataaatat agctgtgagg gaaatatata caaatatatt
     1681 ggagcaaata aattgtacat acaaatattt attactaatt tctattgaga cgaaatgaac
     1741 cactcggaac catttgagcg aaccgaatcg cgcggaacta acgacagtcg ctccaaggtc
     1801 gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa gagcaactac
     1861 gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa agtatgtata
     1921 taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt aaggtatgca
     1981 ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg atgttcgaag
     2041 gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat ataattacat
     2101 tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa
     2161 caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc
     2221 cagatatgcg agcacccgga agctcacgat gagaatggcc agacccacgt agtccagcgg
     2281 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     2341 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     2401 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     2461 caccgagccc gagttcaaga agaagccgcc aaagagcagg aatggtatga taaccggcgg
     2521 acccacagac agcgccatcg aggtcgagga gctggcgcag gatattagat atccgaagga
     2581 cgttgacaca ttggccacca gagtgaccag cgccaggcag ttgaagaagt gcagcactcc
     2641 ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag accagtggca ctgtgagaaa
     2701 aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat aaagtcgact
     2761 tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg aatcgattca
     2821 ttagaaagtt agtaaattat tgaaatgcaa atgtattcta aacatgactt acatttatcg
     2881 tggcaaagac gttttgaaag gtcatgttgg tcaggaagag gaagatggct ccgttgatat
     2941 tcatcacacc cacttgcgtg agttgttggc ccaaaaagat gaggccaatc aagatggcaa
     3001 ccatctgcaa attaaaatgt tactcgcatc tcattaatat tcgcgagtta aatgaaattt
     3061 atttatcttc tgcaaaacta taaactatac atctcattga aaaaaactaa gaagggtgtg
     3121 gaatcaggca attctatcta aaatctagcg aatttgtttc caagaattgt aagcgttata
     3181 tcatttgttt ccactggaac cactcaccgt tgtctgaata agtcgcactt ttacgaggag
     3241 tggttccttg agcaccgaca gccaggatcg ccacaggacc gcccggaact gcatgaacca
     3301 ggtggccttg taggtgtacc cattctccgg ctgctccagt ggcttctcca gatttttggt
     3361 ggccaacaac tgctccatat cccgggctac tttgctaatg gcaaaattgt cgcatatctt
     3421 ggcgatccga tcacgggact cgatctcccg tccgggcaca acggccaaca cctgtacgta
     3481 aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc acgctggata ggagttgaga
     3541 tgttatgtaa tactagatac ccttaataaa cacatcgaac tcactaggaa aagaagtcga
     3601 cggcttcgct gggagtgccc aagaaagcta ccctgccctc ggccatcaga aggatcttgt
     3661 caaagagctc aaacagctcg gaagacggct gatgaatggt caggatgacg gtcttgccct
     3721 tctgcgacag cttcttcagc acctggacga cgctgtgggc ggtaaaggag tccagtccgg
     3781 aggtgggctc atcgcagatc agaagcggcg gatcggttag agcctcggag gcgaatgcca
     3841 gacgcttcct ttctccgccg gacagacctt tcaccctgcc gggcacaccg atgatcgtgt
     3901 gctgacattt gctgagcgaa agctcctgga tcacctgatc cacgcgggcc actcgctgcc
     3961 gataggtcag atgtcgtggc atccgcacca tggcttggaa aatcaggtgt tccctggccg
     4021 ttagggagcc gataaagagg tcatcctgct ggacataggc gcacctggcc tgcatctcct
     4081 tggcgtccac aggttggcca ttgagcagtc gcatcccgga tggcgatact tggatgccct
     4141 gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt tccggcaccg gaactgccca
     4201 tcacggccaa aagttcgccc ggataggcca cgccgcaaac tgagtttcaa attggtaatt
     4261 ggacccttta ttaagatttc acacagatca gccgactgcg aatagaaact caccgttctt
     4321 gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg cagaatagtc cgcgtgtccg
     4381 gttgaccagc tgccgccatc cggagcccgg ctgattgacc gccccaaaga tgtccatatt
     4441 gtgccaggca taggtgaggt tctcggctag ttggccgctc cctgaaccgg agtcctccgg
     4501 cggactgggt ggccggagcg tgccgtagtt tttggcctgc ccgaagccct ggttaatgca
     4561 gctctgcgaa gccgctccgc tgtcaccctg caatgatagg ggatctcaaa tatcaactac
     4621 aagcgttatg ctcatctaac cccgaacaaa aagtaccccg aagtatccta cgaagtaggt
     4681 ttatactttt atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat
     4741 tttttgtaaa aaagaatata gtcgaaaatg aatgccttta gatgtcttga tcatgatatg
     4801 atctcaaaaa ttgtcttata tagcgagaac agctaccaga ataatctgtt tcgtgtcact
     4861 atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca
     4921 atttgatgtt gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta
     4981 ctactcacat tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc
     5041 tcctgatcct cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa
     5101 aaccaatcac caccccaatc actcaaaaaa caaacaaaaa taagaagcga gaggagtttt
     5161 ggcacagcac tttgtgttta attgatggcg taaaccgctt ggagcttcgt cacgaaaccg
     5221 ctgacaaaat gcaactgaag gcggacattg acgctacgta acgctacaaa cggtggcgaa
     5281 agagatagcg gacgcagcgg cgaaagagac ggcgatattt ctgtggacag agaaggaggc
     5341 aaacagcgct gactttgagt ggaatgtcat tttgagtgag aggtaatcga aagaacctgg
     5401 tacatcaaat acccttggat cgaagtaaat ttaaaactga tcagataagt tcaatgatat
     5461 ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt tccgcaaaaa tgggttttat
     5521 taacttacat acatactagg aagcatacta gcgtgccgca aaaatatact aaaataaata
     5581 ccgttcagac atggattaat gcgacttact ctatggtttg tgactttact aatgttttta
     5641 gctaagttca tacactgaga aaaattatta cttaatgtta aatgaatatt aaattaaaaa
     5701 tagagttcca atcccaagcc ccaatcaccg cgcaaaaata cttcaagacc acattaaaca
     5761 tttattaaaa tctcacatac aaattcaaat ccacatgaca taaaacagtt taaaattagc
     5821 agaaatccta caggatataa aatcattact tgtacagctc gtccatgccg agagtgatcc
     5881 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     5941 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     6001 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     6061 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     6121 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     6181 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     6241 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     6301 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     6361 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     6421 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     6481 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     6541 acagctcctc gcccttgctc accatggcgg ccgctccact tccgccgaag aattgcttgg
     6601 ccagttggat aatattcatg gccaatcgga tctcccgggc gccgcgaacg cccagtgccg
     6661 ccaagtcctc cagggtcatt tggatgagca cctcgatgtc gatctcctgg cgcgtgaaca
     6721 gctctgtgag atgcgcgttt ccggtgcgat cgagcaggga tttgacccga ggagccaaag
     6781 tggggtgctc caccggggcg cagggcttat agcgtgaatg atggatactg ggagcgggag
     6841 ctcttgggtc ctccacttta atctttcgcg cacttttcaa cttggattcc tcctgcctca
     6901 gccgcgtttc ctgctgcagt cggatatccg gctttggtct gtcttctctc ttagttctca
     6961 gtagtttcga atctccagat tctcccagta gcctggtctc cccatttata agggtacttt
     7021 gacttgtggg cgcaaatacg gaacgcgtgc gcataaattt ctgtgaaaat cgacggggca
     7081 aagtggattc cgatagtcgt tttaacccgg acaatgccgg tcgactgtta ggaaccgtaa
     7141 gattattgtt cttcggcatt ccaaagagtc cgtatctcgc ggtacccttc ctgccaacgc
     7201 ctccagtggg cgcgacatct tcgaatgtaa aagcttcggt ggagagggga gcgagtgggg
     7261 ccagtggggc tgcgggatct attgaaagtt aaaggatata ttttaaactt gtttacaaaa
     7321 gtattagcaa tactacctct atagacgcga ttgtagcgct ccattcggta attttacaaa
     7381 taatattttc cacgagcaca aaaaagacga caaaaaaaaa cgtgcaataa gatttgcaaa
     7441 atgcaactaa ctgtaattaa ctgctttttt aatgcgaccg cattaagagc ggttaaaatt
     7501 accgaaaatt ctgtgatcag gcaactcata aaatcctagt catagaacca tgatcagtgc
     7561 tgaaaaacga tccttaaaaa tagatcatat aatgagtttt taagatctaa attacaatat
     7621 gtatatttct tttcaagaaa ataaatttca acaacagtca caactatcac aaagtgctta
     7681 aaaatatgat ataagaatta tgcctaataa ctaattgtaa actacaatag taaaaaataa
     7741 ttcataaaaa actcgagagt ttagaaacta tataaaaatg tgttttgttt ctgttaaggt
     7801 ttttgtgcct gtttataatt gattaagaat aaacgttaat ctggcacatt agaatattca
     7861 tatccatgtt tttttgctat tgttaaatct ctgactttac cctcgtgatt tgggtgaaat
     7921 aaataaatta gaataaaagt tcaaggaact cgccacgcat taatatttac catttcacat
     7981 gtctaagtac caaatatcgt ttttataccg tctacataat attattttgc ttctaattaa
     8041 agcgagtttg tccagttttt cagtcgttac ttttgcggtg cgatttgatt tgatttgctt
     8101 cggtttttgc gtcgaagttt ttgcgacaaa tctttttttt gcgaattggc agtcaacaga
     8161 tgaaccgata aaatgtaaac aattgttgca caaaacgtgc aaattaattt aacgaaaata
     8221 atcaattaag ttaatttaat gacgccgaaa attcacaatt cttgcgtgca ataatgacaa
     8281 tattcataaa gagcacttgg agtaaaaatt gacaattttt tttatgaaaa tgcctaacca
     8341 taaaacataa aaaattttac attttgttat aaaatttgca cagtaattta ttacttcaat
     8401 agtcgtttgc tgtgcaaatt aatgtattta ttgaaaaata ctttttcatg tcatctgata
     8461 atcatttaaa catatattaa ttgtttcgct atgcatgaaa aagaaaatat tacagctaaa
     8521 tactaattta aacaaattaa tagttaggaa actgtaatat tgtattggtt atgctctaaa
     8581 atataaatac attttatttt tgtcaaatgc ttagcgtgca cttcttacta tataatatat
     8641 tttttttatg aaaccttaag taaggcttac ttactaaaga ttcggaacca ttttcaaaac
     8701 cagtgacttc agataaatag atatctatat ttatctatgt atgtaaagat atattatttt
     8761 gtgtactgtg aaatcatgac attgccacct gttaaagccc cacagatatt ttggcgctgc
     8821 tgctgctgtt gtttgggttt taggcgctta ccttgcttta tttggttttt cgtgggcaag
     8881 aacaacatgg aattattgtc tttgcgcagt cggcaatgcc gaagctttgc aaacccccca
     8941 ccccccctgg cgaccaataa actcccccct ttcggctaag ttggccacat gtggggcttg
     9001 ttgttgccgg cttttggcta agaaacagct gagctcggcc agctgctttg tgtttatatt
     9061 tttttcacga gcatcgaagc gcctgcgcac tagccataca tatatatact tatatgcata
     9121 tacatatata gagccccccg atcccgaaag ccgaagcgga aagccgagct ttttgtcgac
     9181 tcgagaacgg acgtctctag agtcgacctc gaacgttaac gttaacgtaa cgttaactcg
     9241 aggcctcgag gtcgacctgc agccaagctt tgcgtactcg caaattatta aaaataaaac
     9301 tttaaaaata atttcgtcta attaatatta tgagttaatt caaaccccac ggacatgcta
     9361 agggttaatc aacaatcata tcgctgtctc actcagactc aatacgacac tcagaatact
     9421 attcctttca ctcgcactta ttgcaagcat acgttaagtg gatgtctctt gccgacggga
     9481 ccaccttatg ttatttcatc atggtctggc cattctcatc gtgagcttcc gggtgctcgc
     9541 atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa
     9601 ctgcttgttt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa
     9661 aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt
     9721 atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt
     9781 acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata
     9841 ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc
     9901 acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt
     9961 gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggtgcgg tatttcacac
    10021 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
    10081 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
    10141 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
    10201 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    10261 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10321 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10381 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    10441 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    10501 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10561 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    10621 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
    10681 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    10741 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    10801 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    10861 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    10921 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
    10981 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    11041 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    11101 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    11161 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    11221 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    11281 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    11341 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    11401 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
