LOCUS       sequence-217775-        5464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa230 to C-terminus of Cre recombinase fused to pMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2690_pCAG-PV1-pMag-L1-iCre-230C-BGHpA
FEATURES             Location/Qualifiers
     source          1..5464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1514..1534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1544..1768
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2008..2027)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2073..2128
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2127..2146)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2490..2506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2490..2506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2503..2525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2514..2530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2538..2544,2545..2562,2563..2568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2583..2604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2689..2823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2843..2977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2880..2899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2934..2953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3045..3062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3216..3804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3296..3315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3975..4766,4767..4835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4598..4617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4836..4940)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4971..5350
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgc atactcttta tgcccccggt ggatatgaca ttatgggata
      721 tctggaccag atcggcaacc ggccaaaccc gcaggtggaa ctgggccccg tggatacatc
      781 ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt acgcgagtga
      841 agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga attgccggtt
      901 tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg tggatagtaa
      961 cacaatcaat actatgcgca aggcaatcga caggaatgcc gaggtgcagg ttgaagtagt
     1021 caattttaaa aagaatggac agcgatttgt taatttcctg actatgatac ctgttaggga
     1081 cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaat ccggagggtc
     1141 tggctccgga tcaagtggtg gcagcggtac cgtggctgat gaccccaaca actacctgtt
     1201 ctgccgggtc agaaagaatg gtgtggctgc cccttctgcc acctcccaac tgtccacccg
     1261 ggccctggaa gggatctttg aggccaccca ccgcctgatc tatggtgcca aggatgactc
     1321 tgggcagaga tacctggcct ggtctggcca ctctgccaga gtgggtgctg ccagggacat
     1381 ggccagggct ggtgtgtcca tccctgaaat catgcaggct ggtggctgga ccaatgtgaa
     1441 cattgtgatg aactacatca gaaacctgga ctctgagact ggggccatgg tgaggytscw
     1501 cgacgaarcr gaccccaaga aaaagcggaa ggtgtgagaa ttcctgtgcc ttctagttgc
     1561 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1621 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1681 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     1741 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     1801 agggggtatc cccacgcgcc ctgtagcgtg taccgctggg agttcgtaga cggaaacaaa
     1861 cgcagaatcc aagcgcggcc gcccaggata catagattac cacaactccg agcccttcca
     1921 ccgctagcgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     1981 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     2041 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     2101 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2161 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     2221 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     2281 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     2341 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     2401 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     2461 cctgcagccc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2521 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2581 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2641 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     2701 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2821 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     2881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2941 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     3001 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3061 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4081 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt
     4981 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5041 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     5101 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     5161 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     5221 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     5281 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5341 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     5401 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     5461 gggg
//
