LOCUS       sequence-217777-        5248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa252 to C-terminus of Cre recombinase fused to pMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA
FEATURES             Location/Qualifiers
     source          1..5248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     317..339
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     401..420
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1299..1319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1329..1553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1793..1812)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1858..1913
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1912..1931)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2274..2290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2274..2290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2287..2309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2298..2314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2322..2328,2329..2346,2347..2352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2367..2388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2473..2607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2627..2761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2664..2683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2718..2737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2829..2846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3000..3588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3080..3099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3759..4550,4551..4619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4382..4401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4620..4724)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4755..5134
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
       61 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
      121 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
      181 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
      241 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
      301 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
      361 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
      421 ctgtctcatc attttggcaa agaattgatt aattcgagcg aaggcgcgcc cattactcgc
      481 atccattctc aggctgtctc gtctcgtctc gcacgcgtgc cgccaccatg catactcttt
      541 atgcccccgg tggatatgac attatgggat atctgaggca gatcaggaac cggccaaacc
      601 cgcaggtgga actgggcccc gtggatacat cctgcgcctt gattctttgt gacctgaaac
      661 agaaagacac cccgatagtt tacgcgagtg aagccttcct ctacatgaca ggttacagca
      721 acgcagaggt gctgggccgg aattgccggt ttctgcaaag ccctgacggc atggtgaagc
      781 ccaagagcac ccggaagtac gtggatagta acacaatcaa tactatgcgc aaggcaatcg
      841 acaggaatgc cgaggtgcag gttgaagtag tcaattttaa aaagaatgga cagcgatttg
      901 ttaatttcct gactatgata cctgttaggg acgaaacagg cgagtatcga tactctatgg
      961 gattccagtg cgaaacagaa tccggagggt ctggctccgg atcaagtggt ggcagcggta
     1021 ccgccacctc ccaactgtcc acccgggccc tggaagggat ctttgaggcc acccaccgcc
     1081 tgatctatgg tgccaaggat gactctgggc agagatacct ggcctggtct ggccactctg
     1141 ccagagtggg tgctgccagg gacatggcca gggctggtgt gtccatccct gaaatcatgc
     1201 aggctggtgg ctggaccaat gtgaacattg tgatgaacta catcagaaac ctggactctg
     1261 agactggggc catggtgagg ctgctcgagg atggggaccc caagaaaaag cggaaggtgt
     1321 gagaattcct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1381 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1441 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     1501 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     1561 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcgtgtaccg
     1621 ctgggagttc gtagacggaa acaaacgcag aatccaagcg cggccgccca ggatacatag
     1681 attaccacaa ctccgagccc ttccaccgct agcgcactcc tcaggtgcag gctgcctatc
     1741 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     1801 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     1861 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     1921 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     1981 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     2041 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     2101 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     2161 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     2221 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     2281 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2341 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2401 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     2461 cagcaaccat agtcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2521 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     2581 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
     2641 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2701 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     2761 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2881 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2941 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3421 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4741 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     4801 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     4861 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     4921 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     4981 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     5041 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     5101 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     5161 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     5221 attttgtgca gcgatggggg cggggggg
//
