LOCUS       sequence-217807-        7333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid suitable for experiments in environments where 
            there is no antibiotic selection present. Constitutive promoter pNeo
            expressing sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOPS0377
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(946..1032)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1437..2150
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2167..2253
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2346..2440
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2550..3365
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3516..3624
                     /note="incP origin of transfer"
     CDS             complement(3685..4833)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      5226..5757
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ccagggcatg gcgtgggcct atcgcttcca cggccgcgcg gccgaccctg aaatgctgcg
       61 gctcgaacag gaggcgcgag gcaagcgcgt cggcctctgg tccgatccgc acgccgtcga
      121 gccgtggaaa tggcgacgcg agagcaacaa ccggagggac gaaggttgaa ggtcgcccgc
      181 atctacctgc gcgccagtac ggacgagcag aatcttgaac gccaggagag ccttgtagcg
      241 gccacgcggg ccgccgggta ctacgtcgcc ggcatctacc gcgagaaggc gtccggcgca
      301 cgcgccgacc ggcccgagct gctgcgcatg atcgcggacc tgcaacctgg tgaagtcgtc
      361 gttgcggaga agatcgaccg catcagccgc ttgccgttgg ccgaggccga gcgcctggtt
      421 gcgtcgatcc gggccaaagg ggccaagctg gccgtgcctg gcgtggtgga cctgtcggag
      481 ctggccgccg aggcgaacgg agtggcgaaa atcgttctgg aatccgtcca ggacatgctt
      541 ttgaagctcg ccttgcagat ggcccgcgac gactacgagg atcggcgcga gcgtcaacgt
      601 cagggtgtcc agttggcgaa ggccgccggc cgctacaccg gccgcaaacg tgacgccggc
      661 atgcacgacc gcatcatcac gcttcgctcc ggcggatcga gcattgccaa gacggccaag
      721 ctggtcggat gcagcccgag ccaggtcaaa cgagtgtggg cggcctggaa cgcgcagcag
      781 caaaaataaa gccgggcagt gcccggcttt tctcaccttt tcgcgtcccg cagggccgct
      841 gcgagcgccc tacctagatc ctcgctttcc ccctcggtgt agtccggcca gggcacgaag
      901 ggcgcggatg cgaacctgtt gagcaggtca gatctagggc ggcggatttg tcctactcag
      961 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     1021 tcgttttatt tgatgcctgc cgaattcgga tccggagagg cctgaatcgc cccatcatcc
     1081 agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg
     1141 attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga
     1201 tccttcaact cagcaaaagt tcgatttatt caacaaagcg acgttgtgtc tcaaaatctc
     1261 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1321 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgaga
     1381 tctatcgatg catgccatgg tacccgggag ctcgaattca ggcttggagg atacgaatgc
     1441 gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg
     1501 tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac
     1561 tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg actctggtaa
     1621 cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg
     1681 acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg
     1741 atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc
     1801 gcattgagct gaaaggcatt gactttaaag aagatggcaa tatcctgggc cataagctgg
     1861 aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa aatggcatta
     1921 aagcgaattt taaaattcgc cacaacgttg aggatggcag cgtgcagctg gctgatcact
     1981 accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat cactatctga
     2041 gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg gttctgctgg
     2101 agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa tgagcttccc
     2161 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2221 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     2281 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag ggaattccgc
     2341 tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
     2401 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggcaatt gtgtctcaaa
     2461 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     2521 cttacataaa cagtaataca aggggtgtta tgagccatat tcagcgtgaa acgagctgta
     2581 gccgtccgcg tctgaacagc aacatggatg cggatctgta tggctataaa tgggcgcgtg
     2641 ataacgtggg tcagagcggc gcgaccattt atcgtctgta tggcaaaccg gatgcgccgg
     2701 aactgtttct gaaacatggc aaaggcagcg tggcgaacga tgtgaccgat gaaatggtgc
     2761 gtctgaactg gctgaccgaa tttatgccgc tgccgaccat taaacatttt attcgcaccc
     2821 cggatgatgc gtggctgctg accaccgcga ttccgggcaa aaccgcgttt caggtgctgg
     2881 aagaatatcc ggatagcggc gaaaacattg tggatgcgct ggccgtgttt ctgcgtcgtc
     2941 tgcatagcat tccggtgtgc aactgcccgt ttaacagcga tcgtgtgttt cgtctggccc
     3001 aggcgcagag ccgtatgaac aacggcctgg tggatgcgag cgattttgat gatgaacgta
     3061 acggctggcc ggtggaacag gtgtggaaag aaatgcataa actgctgccg tttagcccgg
     3121 atagcgtggt gacccacggc gattttagcc tggataacct gattttcgat gaaggcaaac
     3181 tgattggctg cattgatgtg ggccgtgtgg gcattgcgga tcgttatcag gatctggcca
     3241 ttctgtggaa ctgcctgggc gaatttagcc cgagcctgca aaaacgtctg tttcagaaat
     3301 atggcattga taatccggat atgaacaaac tgcaatttca tctgatgctg gatgaatttt
     3361 tctaataatt aattgaacta cttttccgct gcataaccct gcttcggggt cattatagcg
     3421 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     3481 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     3541 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     3601 aatcctgctc tgcgaggctg gccgtaggcc ggccgcgatg caggtggctg ctgaaccccc
     3661 agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca
     3721 ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg
     3781 ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag
     3841 cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag
     3901 cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat
     3961 ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa
     4021 gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc
     4081 cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga
     4141 ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt
     4201 gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc
     4261 gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag
     4321 cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt
     4381 tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc
     4441 cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcttcgc tgacctgttt
     4501 tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat
     4561 ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgagc cgacgcgaac gctccacggc
     4621 ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc
     4681 cgtagcttgc tggactatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac
     4741 ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg
     4801 cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgga
     4861 attaattccc cggatcgatc cgtcgatctt gatcccctgc gccatcagat ccttggcggc
     4921 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     4981 ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc
     5041 ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag
     5101 tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtggctgcc
     5161 atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc
     5221 gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca
     5281 cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg
     5341 ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt
     5401 tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg
     5461 cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca
     5521 ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa
     5581 tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc
     5641 ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc
     5701 ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggcccta
     5761 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatcc cccgcagaaa
     5821 aaaaggatct caagaagatc ctttgatctt ttctacatta ctgcatgagc ttgtggaagt
     5881 gtgctgagca taaatgattc tcctagctgt tctttgggta agtacgccat caggacgttg
     5941 tgagtggcgc gatttttagc ggctgaaatc agcccttgag cctgtcggca agtcgcgtca
     6001 tgaggtccat gcgctcatgc aggatcgcca cgaccaacgc gggttcgccc gcacgcggca
     6061 ggcaaaaaac gtagtggtgt tcgcagcggg ccatccgcag cgcgggaaag agttcgctca
     6121 tgtccttaaa cgggccttcg ccggcggcaa gcctggctat gccctgttcc agcttagcga
     6181 tatagcggcg cacctgcgcc gcgccccact cccggcgcgt gtagcggatg atgccgcgta
     6241 gatcggcttc ggcctcagcc gtgaggatgt aggccgtcaa gcgcgatccc cgctgagttc
     6301 ttcatcaaga atttcgccga cgctcttggt ggacaccttg ccggcaagcc catcgttgat
     6361 gcggttcccc agcatggttt tcagttcctg ccatgcctga tcggcatcag cgtcaccggg
     6421 gaacagacgt tcgagggcgt attgcttaat ggtcttgccc tgcaaggcgg ccagggcttt
     6481 caggctctgg tgctgctggt ccgtcatgtc gattgtcagg cggctcattg gataacctcc
     6541 ataaaataca cgtaaccaca ttagcacata tgtgggcgtg aggctacagc gcgaggcgca
     6601 ttaaggtcgg gaaaatgcgc taggcgcatt taaattgcgt attgctgtaa tgcgccatgc
     6661 cggctagact aggcccaaat gggtataccc aatttgacca agggggacgc gatgagggcg
     6721 gccaagcact accgacaact tctatccatc gacttcaaca tcgaggcgct ggccttcgtg
     6781 cctggacccg acggcacacg cggccggcgc atccacgtcc tggggcgcga ggtccgcgac
     6841 cggcccggcc tggtcgagta cctttcgccg gcgttcggct cgcgggtggc gctggacggc
     6901 tactgcaagg ccaatttcga tgcagtgctg cacctggcgt accccgatca tcagcaatgg
     6961 ggccacgcat gaagcgccga agctacgcca tgctgcgcgc cgctgccgcg ctggccgtcc
     7021 tggtcgttgc ctcgccggca tgggccgagc tgcgcggcga ggtcgtgcgc atcatcgacg
     7081 gcgacaccat cgacgtgctg gtagacaagc agccggtgcg cgtgcgcctg gtggacattg
     7141 acgcgccgga aaagcggcaa gccttcggcg aacgtgcgcg ccaggcgctg gccggcatgg
     7201 tgttccgccg gcacgtcctg gtcgacgaga aggacaccga ccgttacggc cgcacgctgg
     7261 gcaccgtctg ggtcaacatg gagctggcca gccggccgcc gcagccgcgc aacgtcaacg
     7321 ccgcgatggt tca
//
