LOCUS       sequence-217808-        7668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suitable for experiments in environments where there is no 
            antibiotic selection present. Symbiosis-induced nifH promoter from 
            Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing 
            sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOPS0379
FEATURES             Location/Qualifiers
     source          1..7668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(276..362)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1102..1815
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1832..1918
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2011..2105
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2204..2223)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2215..3030
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3181..3289
                     /note="incP origin of transfer"
     CDS             complement(3350..4498)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      4891..5422
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gcatcatcac gcttcgctcc ggcggatcga gcattgccaa gacggccaag ctggtcggat
       61 gcagcccgag ccaggtcaaa cgagtgtggg cggcctggaa cgcgcagcag caaaaataaa
      121 gccgggcagt gcccggcttt tctcaccttt tcgcgtcccg cagggccgct gcgagcgccc
      181 tacctagatc ctcgctttcc ccctcggtgt agtccggcca gggcacgaag ggcgcggatg
      241 cgaacctgtt gagcaggtca gatctagggc ggcggatttg tcctactcag gagagcgttc
      301 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      361 tgatgcctgc cgaattcgga tccggagtcg atgctgaccg cctgatcgcc acaatagacg
      421 acatccgata cgattctccg cttagcgatc tcggctttat cttccacgcg attaacgttg
      481 aacacatgct tcggcgaatt gacggaactt gaaggagatc acccgctatc gcagcgggga
      541 cttcaacacg cctgcaactg gtaacccgtg catcgccgcg gaacgaggcc gatgggttcg
      601 ctgccacttt gaaatgtcgt gcttctggag cgagacttat atgaacattt gccatcgctc
      661 gcgcccagac gccgcgtcaa tgacgtcttc ctcgtgaagt ggatcgctag ccgtggcgta
      721 gtcgagtgac atggcgcttg atcatcgccg gaaacagccc tttgcggcaa gtgatcagaa
      781 ttcgttactg ctggtctggt gatgggatac aggctgttcg aacaatggtc ctagaagcta
      841 cacatggata aatgagacat cctgtcggct ggtcagatat tcgacattgt catcaccttt
      901 gtcggttacg tgacaagcgt tcctgttctc aatcccgcat ctttgcgccc acataactgg
      961 aatcgcagta aattattgtt cgtcatctct atcggcctgc ttggcacgaa tcttgtgagc
     1021 tatagcgagg cagcggaacg gcctccgcat ccacgttgcg ggataaccgc attgcctcga
     1081 acacatgaag gaacgccaaa aatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct
     1141 attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt
     1201 gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg
     1261 ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt
     1321 tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg
     1381 caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa
     1441 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagat
     1501 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc
     1561 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgttgaggat
     1621 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt
     1681 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag
     1741 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg
     1801 gatgaactgt acaaatgagc ttcccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1861 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     1921 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     1981 gccataaact gccagggaat tccgctcttg gactcctgtt gatagatcca gtaatgacct
     2041 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     2101 agaatccagg caattgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2161 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2221 catattcagc gtgaaacgag ctgtagccgt ccgcgtctga acagcaacat ggatgcggat
     2281 ctgtatggct ataaatgggc gcgtgataac gtgggtcaga gcggcgcgac catttatcgt
     2341 ctgtatggca aaccggatgc gccggaactg tttctgaaac atggcaaagg cagcgtggcg
     2401 aacgatgtga ccgatgaaat ggtgcgtctg aactggctga ccgaatttat gccgctgccg
     2461 accattaaac attttattcg caccccggat gatgcgtggc tgctgaccac cgcgattccg
     2521 ggcaaaaccg cgtttcaggt gctggaagaa tatccggata gcggcgaaaa cattgtggat
     2581 gcgctggccg tgtttctgcg tcgtctgcat agcattccgg tgtgcaactg cccgtttaac
     2641 agcgatcgtg tgtttcgtct ggcccaggcg cagagccgta tgaacaacgg cctggtggat
     2701 gcgagcgatt ttgatgatga acgtaacggc tggccggtgg aacaggtgtg gaaagaaatg
     2761 cataaactgc tgccgtttag cccggatagc gtggtgaccc acggcgattt tagcctggat
     2821 aacctgattt tcgatgaagg caaactgatt ggctgcattg atgtgggccg tgtgggcatt
     2881 gcggatcgtt atcaggatct ggccattctg tggaactgcc tgggcgaatt tagcccgagc
     2941 ctgcaaaaac gtctgtttca gaaatatggc attgataatc cggatatgaa caaactgcaa
     3001 tttcatctga tgctggatga atttttctaa taattaattg aactactttt ccgctgcata
     3061 accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata
     3121 tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc
     3181 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
     3241 ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt aggccggccg
     3301 cgatgcaggt ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
     3361 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
     3421 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
     3481 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
     3541 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
     3601 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
     3661 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
     3721 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
     3781 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
     3841 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
     3901 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
     3961 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
     4021 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
     4081 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
     4141 cctgcttggc ttcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
     4201 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
     4261 cgagccgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
     4321 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac tatcgagccg acggactgga
     4381 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
     4441 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
     4501 accctccttg cgggattgcc ccggaattaa ttccccggat cgatccgtcg atcttgatcc
     4561 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
     4621 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc
     4681 ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc
     4741 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
     4801 cggactggct ttctacgtgg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg
     4861 cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg
     4921 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
     4981 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
     5041 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
     5101 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
     5161 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
     5221 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
     5281 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
     5341 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
     5401 ggtatccaca acgccggcgg ccctacatgg ctctgctgta gtgagtgggt tgcgctccgg
     5461 cagcggtcct gatcccccgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5521 cattactgca tgagcttgtg gaagtgtgct gagcataaat gattctccta gctgttcttt
     5581 gggtaagtac gccatcagga cgttgtgagt ggcgcgattt ttagcggctg aaatcagccc
     5641 ttgagcctgt cggcaagtcg cgtcatgagg tccatgcgct catgcaggat cgccacgacc
     5701 aacgcgggtt cgcccgcacg cggcaggcaa aaaacgtagt ggtgttcgca gcgggccatc
     5761 cgcagcgcgg gaaagagttc gctcatgtcc ttaaacgggc cttcgccggc ggcaagcctg
     5821 gctatgccct gttccagctt agcgatatag cggcgcacct gcgccgcgcc ccactcccgg
     5881 cgcgtgtagc ggatgatgcc gcgtagatcg gcttcggcct cagccgtgag gatgtaggcc
     5941 gtcaagcgcg atccccgctg agttcttcat caagaatttc gccgacgctc ttggtggaca
     6001 ccttgccggc aagcccatcg ttgatgcggt tccccagcat ggttttcagt tcctgccatg
     6061 cctgatcggc atcagcgtca ccggggaaca gacgttcgag ggcgtattgc ttaatggtct
     6121 tgccctgcaa ggcggccagg gctttcaggc tctggtgctg ctggtccgtc atgtcgattg
     6181 tcaggcggct cattggataa cctccataaa atacacgtaa ccacattagc acatatgtgg
     6241 gcgtgaggct acagcgcgag gcgcattaag gtcgggaaaa tgcgctaggc gcatttaaat
     6301 tgcgtattgc tgtaatgcgc catgccggct agactaggcc caaatgggta tacccaattt
     6361 gaccaagggg gacgcgatga gggcggccaa gcactaccga caacttctat ccatcgactt
     6421 caacatcgag gcgctggcct tcgtgcctgg acccgacggc acacgcggcc ggcgcatcca
     6481 cgtcctgggg cgcgaggtcc gcgaccggcc cggcctggtc gagtaccttt cgccggcgtt
     6541 cggctcgcgg gtggcgctgg acggctactg caaggccaat ttcgatgcag tgctgcacct
     6601 ggcgtacccc gatcatcagc aatggggcca cgcatgaagc gccgaagcta cgccatgctg
     6661 cgcgccgctg ccgcgctggc cgtcctggtc gttgcctcgc cggcatgggc cgagctgcgc
     6721 ggcgaggtcg tgcgcatcat cgacggcgac accatcgacg tgctggtaga caagcagccg
     6781 gtgcgcgtgc gcctggtgga cattgacgcg ccggaaaagc ggcaagcctt cggcgaacgt
     6841 gcgcgccagg cgctggccgg catggtgttc cgccggcacg tcctggtcga cgagaaggac
     6901 accgaccgtt acggccgcac gctgggcacc gtctgggtca acatggagct ggccagccgg
     6961 ccgccgcagc cgcgcaacgt caacgccgcg atggttcacc agggcatggc gtgggcctat
     7021 cgcttccacg gccgcgcggc cgaccctgaa atgctgcggc tcgaacagga ggcgcgaggc
     7081 aagcgcgtcg gcctctggtc cgatccgcac gccgtcgagc cgtggaaatg gcgacgcgag
     7141 agcaacaacc ggagggacga aggttgaagg tcgcccgcat ctacctgcgc gccagtacgg
     7201 acgagcagaa tcttgaacgc caggagagcc ttgtagcggc cacgcgggcc gccgggtact
     7261 acgtcgccgg catctaccgc gagaaggcgt ccggcgcacg cgccgaccgg cccgagctgc
     7321 tgcgcatgat cgcggacctg caacctggtg aagtcgtcgt tgcggagaag atcgaccgca
     7381 tcagccgctt gccgttggcc gaggccgagc gcctggttgc gtcgatccgg gccaaagggg
     7441 ccaagctggc cgtgcctggc gtggtggacc tgtcggagct ggccgccgag gcgaacggag
     7501 tggcgaaaat cgttctggaa tccgtccagg acatgctttt gaagctcgcc ttgcagatgg
     7561 cccgcgacga ctacgaggat cggcgcgagc gtcaacgtca gggtgtccag ttggcgaagg
     7621 ccgccggccg ctacaccggc cgcaaacgtg acgccggcat gcacgacc
//
