LOCUS       sequence-217817-        6235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa374 of Flp recombinase fused to nMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2673_pCAG-PV1-FlpO-N374-L1-nMag-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2291..2311
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2321..2545
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2785..2804)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2850..2905
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2904..2923)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3266..3282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3266..3282)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3279..3301)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3290..3306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3314..3320,3321..3338,3339..3344))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3359..3380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3465..3599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3619..3753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3656..3675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3710..3729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3821..3838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3992..4580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4072..4091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4751..5542,5543..5611))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5374..5393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5612..5716)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5747..6126
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga gccagttcga catcctgtgc aagacccccc ccaaggtgct
      721 ggtgcggcag ttcgtggaga gattcgagag gcccagcggc gagaagatcg ccagctgtgc
      781 cgccgagctg acctacctgt gctggatgat cacccacaac ggcaccgcca tcaagagggc
      841 caccttcatg agctacaaca ccatcatcag caacagcctg agcttcgaca tcgtgaacaa
      901 gagcctgcag ttcaagtaca agacccagaa ggccaccatc ctggaggcca gcctgaagaa
      961 gctgatcccc gcctgggagt tcaccatcat cccttacaac ggccagaagc accagagcga
     1021 catcaccgac atcgtgtcca gcctgcagct gcagttcgag agcagcgagg aggccgacaa
     1081 gggcaacagc cacagcaaga agatgctgaa ggccctgctg tccgagggcg agagcatctg
     1141 ggagatcacc gagaagatcc tgaacagctt cgagtacacc agcaggttca ccaagaccaa
     1201 gaccctgtac cagttcctgt tcctggccac attcatcaac tgcggcaggt tcagcgacat
     1261 caagaacgtg gaccccaaga gcttcaagct ggtgcagaac aagtacctgg gcgtgatcat
     1321 tcagtgcctg gtgaccgaga ccaagacaag cgtgtccagg cacatctact ttttcagcgc
     1381 cagaggcagg atcgaccccc tggtgtacct ggacgagttc ctgaggaaca gcgagcccgt
     1441 gctgaagaga gtgaacagga ccggcaacag cagcagcaac aagcaggagt accagctgct
     1501 gaaggacaac ctggtgcgca gctacaacaa ggccctgaag aagaacgccc cctaccccat
     1561 cttcgctatc aagaacggcc ctaagagcca catcggcagg cacctgatga ccagctttct
     1621 gagcatgaag ggcctgaccg agctgacaaa cgtggtgggc aactggagcg acaagagggc
     1681 ctccgccgtg gccaggacca cctacaccca ccagatcacc gccatccccg accactactt
     1741 cgccctggtg tccaggtact acgcctacga ccccatcagc aaggagatga tcgccctgtc
     1801 cggagggtct ggctccggat caagtggtgg cagcggtacc catactcttt atgcccccgg
     1861 tggatatgac attatgggat atctggacca gatcggcaac cggccaaacc cgcaggtgga
     1921 actgggcccc gtggatacat cctgcgcctt gattctttgt gacctgaaac agaaagacac
     1981 cccgatagtt tacgcgagtg aagccttcct ctacatgaca ggttacagca acgcagaggt
     2041 gctgggccgg aattgccggt ttctgcaaag ccctgacggc atggtgaagc ccaagagcac
     2101 ccggaagtac gtggatagta acacaatcaa tactatgcgc aaggcaatcg acaggaatgc
     2161 cgaggtgcag gttgaagtag tcaattttaa aaagaatgga cagcgatttg ttaatttcct
     2221 gactatgata cctgttaggg acgaaacagg cgagtatcga tactctatgg gattccagtg
     2281 cgaaacagaa cccaagaaaa agcggaaggt gtgagaattc ctgtgccttc tagttgccag
     2341 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2401 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2461 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2521 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     2581 gggtatcccc acgcgccctg tagcgtgtac cgctgggagt tcgtagacgg aaacaaacgc
     2641 agaatccaag cgcggccgcc caggatacat agattaccac aactccgagc ccttccaccg
     2701 ctagcgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     2761 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2821 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2881 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     2941 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     3001 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     3061 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     3121 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3181 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     3241 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3421 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3541 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3601 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3661 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3721 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     3781 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3901 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3961 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4021 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4081 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4141 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4201 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4261 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4321 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4381 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4441 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4501 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca ttgattattg
     5761 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5821 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5881 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5941 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6001 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6061 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6121 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     6181 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcgg
//
