LOCUS       sequence-21782-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Cai Y, Jin J, Florens L, Swanson SK, Kusch T, Li B, Workman JL, 
            Washburn MP, Conaway RC, Conaway JW
  TITLE     The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 
            histone acetyltransferase and SRCAP complexes.
  JOURNAL   J Biol Chem. 2005 Apr 8. 280(14):13665-70.
  PUBMED    15647280
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Apr 8. 280(14):13665-70."
COMMENT     Sequence Label: FLAG-ZnF/HIT1
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(637..654)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcatgc cagactacaa ggacgatgat gacaagctcg
      121 agatggtgga gaagaaaact tcggttcgct cccaggaccc cgggcagcgg cgggtgctgg
      181 accgggctgc ccggcagcgt cgcatcaacc ggcagctgga ggccctggag aatgacaact
      241 tccaggatga cccccacgcg ggactccctc agctcggcaa gagactgcct cagtttgatg
      301 acgatgcgga cactggaaag aaaaagaaga aaacccgagg tgatcatttt aaacttcgct
      361 tccgaaaaaa ctttcaggcc ctgttggagg agcagaactt gagtgtggcc gagggcccta
      421 actacctgac ggcctgtgcg ggacccccat cgcggcccca gcgccccttc tgtgctgtct
      481 gtggcttccc atccccctac acctgtgtca gctgcggtgc ccggtactgc actgtgcgct
      541 gtctggggac ccaccaggag accaggtgtc tgaagtggac tgtgtgagga tccggtaccg
      601 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
      661 agc
//
