LOCUS       sequence-217830-        9102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of GFP (generated 
            from plasmid #20321).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: FUW-TetON-GFP
FEATURES             Location/Qualifiers
     source          1..9102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        455..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             671..851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    898..1023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1516..1749
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1934..1978
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2276..2393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2446..2464,2465..2487,2488..2506,2507..2529,2530..2548,
                     2549..2571,2572..2590,2591..2613,2614..2632,2633..2655,
                     2656..2674,2675..2697,2698..2716)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2749..2786
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2783..2807
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2886..2888,2889..2891,2892..3605)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2886..2888,2889..2891,2892..3602)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2931..2952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3192..3211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3539..3560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3630..4218
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3683..3703)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4101..4112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4221..4237)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4222..4238)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4743..4923
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4949..4966)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4955..5179
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5225..5653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5312..5331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5522..5543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5662..5682)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5667..5996
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5847..5982
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5909..5928
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6044..6091
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6110..6484
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6614..6735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6651..6670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6705..6724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6784..6800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6784..6800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6797..6819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6808..6824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6832..6838,6839..6856,6857..6862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6877..6898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7015..7032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7186..7774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7266..7285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7945..8736,8737..8805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8568..8587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8806..8910)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8985..9004)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      661 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      721 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      781 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      841 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      901 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      961 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1021 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1081 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1141 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1201 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1261 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1321 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1381 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1441 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1501 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1561 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1621 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1681 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1741 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1801 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1861 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1921 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1981 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2041 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2101 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2161 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2221 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2281 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2341 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2401 attacaggga cagcagagat ccagtttggt tatcgagttt accactccct atcagtgata
     2461 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2521 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2581 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2641 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2701 ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt aggcgtgtac
     2761 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     2821 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg
     2881 aattcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2941 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3001 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3061 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3121 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3181 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3241 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3301 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3361 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3421 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3481 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3541 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3601 agtaagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3661 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3721 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3781 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3841 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3901 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3961 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4021 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4081 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4141 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4201 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
     4261 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     4321 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     4381 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4441 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     4501 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     4561 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     4621 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     4681 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     4741 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4801 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4861 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4921 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4981 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5041 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5101 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5161 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5221 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5281 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5341 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5401 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5461 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5521 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5581 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5641 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5701 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5761 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5821 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5881 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     5941 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6001 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     6061 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     6121 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     6181 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     6241 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     6301 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     6361 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     6421 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     6481 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6541 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6601 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6661 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6721 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6781 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6901 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6961 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7261 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7381 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7501 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7621 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8941 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9001 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     9061 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg ac
//
