LOCUS       sequence-217831-       11226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RMCE donor vector for inducible expression of SOX10 and GFP and 
            constitutive expression of m2rtTA (generated from plasmid #112668).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 11226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: pZ M2rtTA_CAGG TetON-Sox10 with GFP
FEATURES             Location/Qualifiers
     source          1..11226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2219..3055
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(3065..3098)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    3105..3329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(3343..3351,3352..3387,3388..3390,3391..3426,
                     3427..3429,3430..3465,3466..3471,3472..4089))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4117..4136)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4144..5160
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4198..4220)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(5161..5438)
                     /label=chicken beta-actin promoter
     enhancer        5443..5826
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    5886..5904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5921..5939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5957..5975
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5993..6011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6028..6046
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6064..6082
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6094..6132
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6129..6153
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6161..6170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7592..7648
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7661..7663,7664..7666,7667..8380)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7661..7663,7664..7666,7667..8377)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7706..7727)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7967..7986)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8314..8335
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8401..8420)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8446..8493
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    8748..8859
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(9013..9612)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9096..9116)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9593..9612
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(9622..9675)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9696..9721
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(9725..9758)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    9765..10568
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(10582..10601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10583..10601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10611..10628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10611..10627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10620..10642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10769..11224
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10856..10875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11066..11087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggag
     2221 agcagagcca ggaacccctg tagggaaggg gcaggagagc caggggcatg agatggtgga
     2281 cgaggaaggg ggacagggaa gcctgagcgc ctctcctggg cttgccaagg actcaaaccc
     2341 agaagcccag agcagggcct tagggaagcg ggaccctgct ctgggcggag gaatatgtcc
     2401 cagatagcac tggggactct ttaaggaaag aaggatggag aaagagaaag ggagtagagg
     2461 cggccacgac ctggtgaaca cctaggacgc accattctca caaagggagt tttccacacg
     2521 gacacccccc tcctcaccac agccctgcca ggacggggct ggctactggc cttatctcac
     2581 aggtaaaact gacgcacgga ggaacaatat aaattgggga ctagaaaggt gaagagccaa
     2641 agttagaact caggaccaac ttattctgat tttgtttttc caaactgctt ctcctcttgg
     2701 gaagtgtaag gaagctgcag caccaggatc agtgaaacgc accagacagc cgcgtcagag
     2761 cagctcaggt tctgggagag ggtagcgcag ggtggccact gagaaccggg caggtcacgc
     2821 atccccccct tccctcccac cccctgccaa gctctccctc ccaggatcct ctctggctcc
     2881 atcgtaagca aaccttagag gttctggcaa ggagagagat ggctccagga aatgggggtg
     2941 tgtcaccaga taaggaatct gcctaacagg aggtgggggt tagacccaat atcaggagac
     3001 taggaaggag gaggcctaag gatggggctt ttctgtcacc aatcctgtcc ctagtagcgg
     3061 ccgcgaagtt cctatacttt ctagagaata ggaacttctt cgaaccatag agcccaccgc
     3121 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc
     3181 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta
     3241 ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac
     3301 aacagatggc tggcaactag aaggcacagt cgaggcacta gtttacccgg ggagcatgtc
     3361 aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc
     3421 atcggctggg agcatgtcta agtcaaaatc gtcaagggcg tcggccggcc cgccgctttc
     3481 gcactttagc tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg
     3541 ttcggctccc tgccggtcga acagctcaat tgcttgtctc agaagtgggg gcatagaatc
     3601 ggtggtaggt gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca
     3661 gcccagtgta aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc
     3721 ttgctgacac aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg
     3781 ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg agagcacagc ggaatgactt
     3841 ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc agggggcaga agtgggtatg
     3901 atgcctgtcc agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg
     3961 ccagtacagg gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc
     4021 ttcgataccg actccattga gtaattccag agcgccgttt atgactttgc tcttgtccag
     4081 tctagacatg aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag
     4141 gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggctctag agccgccggt
     4201 cacacgccag aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg
     4261 cggacagccc cgaggctgga gagggagaag gggacggcgg cgcggcgacg cacgaaggcc
     4321 ctccccgccc atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg
     4381 ggtgcggcgg cgcctcccag atttcggctc cgcacagatt tgggacaaag gaagtccctg
     4441 cgccctctcg cacgattacc ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc
     4501 gccggcgctc cgggggccgc cgcgcccctc ccccgagccc tccccggccc gaggcggccc
     4561 cgccccgccc ggcaccccca cctgccgcca ccccccgccc ggcacggcga gccccgcgcc
     4621 acgccccgta cggagccccg cacccgaagc cgggccgtgc tcagcaactc ggggaggggg
     4681 gtgcaggggg ggttgcagcc cgaccgacgc gcccacaccc cctgctcacc cccccacgca
     4741 cacaccccgc acgcagcctt tgttcccctc gcagcccccc ccgcaccgcg gggcaccgcc
     4801 cccggccgcg ctcccctcgc gcacactgcg gagcgcacaa agccccgcgc cgcgcccgca
     4861 gcgctcacag ccgccgggca gcgcggagcc gcacgcggcg ctccccacgc acacacacac
     4921 gcacgcaccc cccgagccgc tccccccgca caaagggccc tcccggagcc cctcaaggct
     4981 ttcacgcagc cacagaaaag aaacaagccg tcattaaacc aagcgctaat tacagcccgg
     5041 aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc agagccgggg
     5101 cgggcggcgc gaggcggcgg cggagcgggg cacggggcga aggcagcgcg cagcgactcc
     5161 cgcccgccgc gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg ccataaaagg
     5221 aaactttcgg agcgcgccgc tctgattggc tgccgccgca cctctccgcc tcgccccgcc
     5281 ccgcccctcg ccccgccccg ccccgcctgg cgcgcgcccc cccccccccc ccgcccccat
     5341 cgctgcacaa aataattaaa aaataaataa atacaaaatt gggggtgggg agggggggga
     5401 gatggggaga gtgaagcaga acgtggggct cacctcgacc atcatggtaa tagcgatgac
     5461 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     5521 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     5581 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     5641 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     5701 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     5761 tgggctatga actaatgacc ccgtaattga ttactattaa taactagcta gtcaataatc
     5821 aatgtcgacg atgatgctgt acactcgaaa ctttttcgaa ggtccgaggt tctagacgag
     5881 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
     5941 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     6001 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
     6061 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
     6121 ataagcagag ctcgtttagt gaaccgtcag atcgcttaag gccaccatgg cggaggagca
     6181 ggacctatcg gaggtggagc tgagccccgt gggctcggag gagccccgct gcctgtcccc
     6241 ggggagcgcg ccctcgctag ggcccgacgg cggcggcggc ggatcgggcc tgcgagccag
     6301 cccggggcca ggcgagctgg gcaaggtcaa gaaggagcag caggacggcg aggcggacga
     6361 tgacaagttc cccgtgtgca tccgcgaggc cgtcagccag gtgctcagcg gctacgactg
     6421 gacgctggtg cccatgcccg tgcgcgtcaa cggcgccagc aaaagcaagc cgcacgtcaa
     6481 gcggcccatg aacgccttca tggtgtgggc tcaggcagcg cgcaggaagc tcgcggacca
     6541 gtacccgcac ctgcacaacg ctgagctcag caagacgctg ggcaagctct ggaggctgct
     6601 gaacgaaagt gacaagcgcc ccttcatcga ggaggctgag cggctccgta tgcagcacaa
     6661 gaaagaccac ccggactaca agtaccagcc caggcggcgg aagaacggga aggccgccca
     6721 gggcgaggcg gagtgccccg gtggggaggc cgagcaaggt gggaccgccg ccatccaggc
     6781 ccactacaag agcgcccact tggaccaccg gcacccagga gagggctccc ccatgtcaga
     6841 tgggaacccc gagcacccct caggccagag ccatggccca cccacccctc caaccacccc
     6901 gaagacagag ctgcagtcgg gcaaggcaga cccgaagcgg gacgggcgct ccatggggga
     6961 gggcgggaag cctcacatcg acttcggcaa cgtggacatt ggtgagatca gccacgaggt
     7021 aatgtccaac atggagacct ttgatgtggc tgagttggac cagtacctgc cgcccaatgg
     7081 gcacccaggc cacgtgagca gctactcagc agccggctat gggctgggca gtgccctggc
     7141 cgtggccagt ggacactccg cctggatctc caagccacca ggcgtggctc tgcccacggt
     7201 ctcaccacct ggtgtggatg ccaaagccca ggtgaagaca gagaccgcgg ggccccaggg
     7261 gcccccacac tacaccgacc agccatccac ctcacagatc gcctacacct ccctcagcct
     7321 gccccactat ggctcagcct tcccctccat ctcccgcccc cagtttgact actctgacca
     7381 tcagccctca ggaccctatt atggccactc gggccaggcc tctggcctct actcggcctt
     7441 ctcctatatg gggccctcgc agcggcccct ctacacggcc atctctgacc ccagcccctc
     7501 agggccccag tcccacagcc ccacacactg ggagcagcca gtatatacga cactgtcccg
     7561 gcccgatatc ggtggtggct cggggggagg agcgacaaac tttagcttgc tgaagcaagc
     7621 tggtgacgtt gaggagaatc ccggaccagg attcaccggt atggtgagca agggcgagga
     7681 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     7741 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     7801 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     7861 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     7921 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     7981 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     8041 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     8101 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     8161 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     8221 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     8281 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     8341 cgccgggatc actctcggca tggacgagct gtacaagtaa acgcgtgggg ggaggctaac
     8401 tgaaacacgg aaggagacaa taccgggaag gaacccgcgc tatgacggca ataaaaagac
     8461 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     8521 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     8581 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     8641 cggcaggccc tgccatagct cgagtgctat tgtcttccca atcctccccc ttgctgtcct
     8701 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
     8761 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
     8821 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgggttt
     8881 aaacgggccc tctagagtcg gtgggcctcg ggggcgggtg cggggtcggc ggggccgccc
     8941 cgggtggctt cggtcggagc catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc
     9001 gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     9061 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     9121 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     9181 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     9241 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     9301 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     9361 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     9421 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     9481 ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     9541 ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     9601 gtactcggtc atttcgagcc tagggccggg attctcctcc acgtcaccgc atgttagaag
     9661 acttcctctg ccctctccgc tgccagatct gaggccctgt gggaggaaga gaagaggtca
     9721 ggccgaagtt cctatactat ttgaagaata ggaacttcaa gcttgcccca ctgtggggtg
     9781 gaggggacag ataaaagtac ccagaaccag agccacatta accggccctg ggaatataag
     9841 gtggtcccag ctcggggaca caggatccct ggaggcagca aacatgctgt cctgaagtgg
     9901 acataggggc ccgggttgga ggaagaagac tagctgagct ctcggacccc tggaagatgc
     9961 catgacaggg ggctggaaga gctagcacag actagagagg taaggggggt aggggagctg
    10021 cccaaatgaa aggagtgaga ggtgacccga atccacagga gaacggggtg tccaggcaaa
    10081 gaaagcaaga ggatggagag gtggctaaag ccagggagac ggggtacttt ggggttgtcc
    10141 agaaaaacgg tgatgatgca ggcctacaag aaggggaggc gggacgcaag ggagacatcc
    10201 gtcggagaag gccatcctaa gaaacgagag atggcacagg ccccagaagg agaaggaaaa
    10261 gggaacccag cgagtgaaga cggcatgggg ttgggtgagg gaggagagat gcccggagag
    10321 gacccagaca cggggaggat ccgctcagag gacatcacgt ggtgcagcgc cgagaaggaa
    10381 gtgctccgga aagagcatcc ttgggcagca acacagcaga gagcaagggg aagagggagt
    10441 ggaggaagac ggaacctgaa ggaggcggca gggaaggatc tgggccagcc gtagaggtga
    10501 cccaggccac aagctgcaga cagaaagcgg cacaggccca ggggagagaa tgctggtcag
    10561 agaaagcagg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    10621 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    10681 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    10741 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
    10801 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10861 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10921 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10981 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    11041 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    11101 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    11161 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    11221 atttag
//
