LOCUS       sequence-217832-       10444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RMCE donor vector for inducible expression of SOX10 constitutive 
            expression of m2rtTA (generated from plasmid #112668).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: pZ M2rtTA_CAGG TetON-Sox10
FEATURES             Location/Qualifiers
     source          1..10444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2219..3055
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(3065..3098)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    3110..3334
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(3348..3356,3357..3392,3393..3395,3396..3431,
                     3432..3434,3435..3470,3471..3476,3477..4094))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4150..4169)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4177..5193
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4231..4253)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(5194..5471)
                     /label=chicken beta-actin promoter
     enhancer        5476..5859
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    5919..5937
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5954..5972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5990..6008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6026..6044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6061..6079
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6097..6115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6127..6165
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6162..6186
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6194..6203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7620..7639)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7664..7711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    7966..8077
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8231..8830)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8314..8334)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8811..8830
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8840..8893)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    8914..8939
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(8943..8976)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    8983..9786
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(9800..9819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9801..9819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9829..9846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9829..9845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9838..9860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9987..10442
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10074..10093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10284..10305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggag
     2221 agcagagcca ggaacccctg tagggaaggg gcaggagagc caggggcatg agatggtgga
     2281 cgaggaaggg ggacagggaa gcctgagcgc ctctcctggg cttgccaagg actcaaaccc
     2341 agaagcccag agcagggcct tagggaagcg ggaccctgct ctgggcggag gaatatgtcc
     2401 cagatagcac tggggactct ttaaggaaag aaggatggag aaagagaaag ggagtagagg
     2461 cggccacgac ctggtgaaca cctaggacgc accattctca caaagggagt tttccacacg
     2521 gacacccccc tcctcaccac agccctgcca ggacggggct ggctactggc cttatctcac
     2581 aggtaaaact gacgcacgga ggaacaatat aaattgggga ctagaaaggt gaagagccaa
     2641 agttagaact caggaccaac ttattctgat tttgtttttc caaactgctt ctcctcttgg
     2701 gaagtgtaag gaagctgcag caccaggatc agtgaaacgc accagacagc cgcgtcagag
     2761 cagctcaggt tctgggagag ggtagcgcag ggtggccact gagaaccggg caggtcacgc
     2821 atccccccct tccctcccac cccctgccaa gctctccctc ccaggatcct ctctggctcc
     2881 atcgtaagca aaccttagag gttctggcaa ggagagagat ggctccagga aatgggggtg
     2941 tgtcaccaga taaggaatct gcctaacagg aggtgggggt tagacccaat atcaggagac
     3001 taggaaggag gaggcctaag gatggggctt ttctgtcacc aatcctgtcc ctagtagcgg
     3061 ccgcgaagtt cctatacttt ctagagaata ggaacttctg catttcgaac catagagccc
     3121 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
     3181 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
     3241 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
     3301 caaacaacag atggctggca actagaaggc acagtcgagg cactagttta cccggggagc
     3361 atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca
     3421 agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggc cggcccgccg
     3481 ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag
     3541 gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt gtctcagaag tgggggcata
     3601 gaatcggtgg taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat
     3661 acgcagccca gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag
     3721 aagccttgct gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt
     3781 gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg agaggagagc acagcggaat
     3841 gacttggcgt tgttccgcag aaagtcttgc catgactcgc cttccagggg gcagaagtgg
     3901 gtatgatgcc tgtccagcat ctcgattggc agggcatcga gcagggcccg cttgttcttc
     3961 acgtgccagt acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc
     4021 aggccttcga taccgactcc attgagtaat tccagagcgc cgtttatgac tttgctcttg
     4081 tccagtctag acatcaattg gtatacgtat gcatatttaa atgaattctt tgccaaaatg
     4141 atgagacagc acaataacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg
     4201 aacatggtta gcagaggctc tagagccgcc ggtcacacgc cagaagccga accccgccct
     4261 gccccgtccc ccccgaaggc agccgtcccc ccgcggacag ccccgaggct ggagagggag
     4321 aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct tcctgccggc
     4381 gccgcaccgc ttcgccccgc gcccgctaga gggggtgcgg cggcgcctcc cagatttcgg
     4441 ctccgcacag atttgggaca aaggaagtcc ctgcgccctc tcgcacgatt accataaaag
     4501 gcaatggctg cggctcgccg cgcctcgaca gccgccggcg ctccgggggc cgccgcgccc
     4561 ctcccccgag ccctccccgg cccgaggcgg ccccgccccg cccggcaccc ccacctgccg
     4621 ccaccccccg cccggcacgg cgagccccgc gccacgcccc gtacggagcc ccgcacccga
     4681 agccgggccg tgctcagcaa ctcggggagg ggggtgcagg gggggttgca gcccgaccga
     4741 cgcgcccaca ccccctgctc acccccccac gcacacaccc cgcacgcagc ctttgttccc
     4801 ctcgcagccc cccccgcacc gcggggcacc gcccccggcc gcgctcccct cgcgcacact
     4861 gcggagcgca caaagccccg cgccgcgccc gcagcgctca cagccgccgg gcagcgcgga
     4921 gccgcacgcg gcgctcccca cgcacacaca cacgcacgca ccccccgagc cgctcccccc
     4981 gcacaaaggg ccctcccgga gcccctcaag gctttcacgc agccacagaa aagaaacaag
     5041 ccgtcattaa accaagcgct aattacagcc cggaggagaa gggccgtccc gcccgctcac
     5101 ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg
     5161 gggcacgggg cgaaggcagc gcgcagcgac tcccgcccgc cgcgcgcttc gctttttata
     5221 gggccgccgc cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc cgctctgatt
     5281 ggctgccgcc gcacctctcc gcctcgcccc gccccgcccc tcgccccgcc ccgccccgcc
     5341 tggcgcgcgc cccccccccc cccccgcccc catcgctgca caaaataatt aaaaaataaa
     5401 taaatacaaa attgggggtg gggagggggg ggagatgggg agagtgaagc agaacgtggg
     5461 gctcacctcg accatcatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     5521 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     5581 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     5641 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     5701 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     5761 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     5821 tgattactat taataactag ctagtcaata atcaatgtcg acgatgatgc tgtacactcg
     5881 aaactttttc gaaggtccga ggttctagac gagtttactc cctatcagtg atagagaacg
     5941 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     6001 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     6061 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     6121 tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     6181 cagatcgctt aaggccacca tggcggagga gcaggaccta tcggaggtgg agctgagccc
     6241 cgtgggctcg gaggagcccc gctgcctgtc cccggggagc gcgccctcgc tagggcccga
     6301 cggcggcggc ggcggatcgg gcctgcgagc cagcccgggg ccaggcgagc tgggcaaggt
     6361 caagaaggag cagcaggacg gcgaggcgga cgatgacaag ttccccgtgt gcatccgcga
     6421 ggccgtcagc caggtgctca gcggctacga ctggacgctg gtgcccatgc ccgtgcgcgt
     6481 caacggcgcc agcaaaagca agccgcacgt caagcggccc atgaacgcct tcatggtgtg
     6541 ggctcaggca gcgcgcagga agctcgcgga ccagtacccg cacctgcaca acgctgagct
     6601 cagcaagacg ctgggcaagc tctggaggct gctgaacgaa agtgacaagc gccccttcat
     6661 cgaggaggct gagcggctcc gtatgcagca caagaaagac cacccggact acaagtacca
     6721 gcccaggcgg cggaagaacg ggaaggccgc ccagggcgag gcggagtgcc ccggtgggga
     6781 ggccgagcaa ggtgggaccg ccgccatcca ggcccactac aagagcgccc acttggacca
     6841 ccggcaccca ggagagggct cccccatgtc agatgggaac cccgagcacc cctcaggcca
     6901 gagccatggc ccacccaccc ctccaaccac cccgaagaca gagctgcagt cgggcaaggc
     6961 agacccgaag cgggacgggc gctccatggg ggagggcggg aagcctcaca tcgacttcgg
     7021 caacgtggac attggtgaga tcagccacga ggtaatgtcc aacatggaga cctttgatgt
     7081 ggctgagttg gaccagtacc tgccgcccaa tgggcaccca ggccatgtga gcagctactc
     7141 agcagccggc tatgggctgg gcagtgccct ggccgtggcc agtggacact ccgcctggat
     7201 ctccaagcca ccaggcgtgg ctctgcccac ggtctcacca cctggtgtgg atgccaaagc
     7261 ccaggtgaag acagagaccg cggggcccca ggggccccca cactacaccg accagccatc
     7321 cacctcacag atcgcctaca cctccctcag cctgccccac tatggctcag ccttcccctc
     7381 catctcccgc ccccagtttg actactctga ccatcagccc tcaggaccct attatggcca
     7441 ctcgggccag gcctctggcc tctactcggc cttctcctat atggggccct cgcagcggcc
     7501 cctctacacg gccatctctg accccagccc ctcagggccc cagtcccaca gccccacaca
     7561 ctgggagcag ccagtatata cgacactgtc ccggccctaa acgcgtgggg gaggctaact
     7621 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     7681 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     7741 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     7801 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     7861 gcaggccctg ccatagctcg agtgctattg tcttcccaat cctccccctt gctgtcctgc
     7921 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     7981 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     8041 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     8101 acgggccctc tagagtcggt gggcctcggg ggcgggtgcg gggtcggcgg ggccgccccg
     8161 ggtggcttcg gtcggagcca tggggtcgtg cgctcctttc ggtcgggcgc tgcgggtcgt
     8221 ggggcgggcg tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca
     8281 cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg
     8341 cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca
     8401 cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga
     8461 accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg
     8521 ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg
     8581 cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca
     8641 ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct
     8701 cgatgtggcg gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg
     8761 cgagggtgcg tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt
     8821 actcggtcat ttcgagccta gggccgggat tctcctccac gtcaccgcat gttagaagac
     8881 ttcctctgcc ctctccgctg ccagatctga ggccctgtgg gaggaagaga agaggtcagg
     8941 ccgaagttcc tatactattt gaagaatagg aacttcaagc ttgccccact gtggggtgga
     9001 ggggacagat aaaagtaccc agaaccagag ccacattaac cggccctggg aatataaggt
     9061 ggtcccagct cggggacaca ggatccctgg aggcagcaaa catgctgtcc tgaagtggac
     9121 ataggggccc gggttggagg aagaagacta gctgagctct cggacccctg gaagatgcca
     9181 tgacaggggg ctggaagagc tagcacagac tagagaggta aggggggtag gggagctgcc
     9241 caaatgaaag gagtgagagg tgacccgaat ccacaggaga acggggtgtc caggcaaaga
     9301 aagcaagagg atggagaggt ggctaaagcc agggagacgg ggtactttgg ggttgtccag
     9361 aaaaacggtg atgatgcagg cctacaagaa ggggaggcgg gacgcaaggg agacatccgt
     9421 cggagaaggc catcctaaga aacgagagat ggcacaggcc ccagaaggag aaggaaaagg
     9481 gaacccagcg agtgaagacg gcatggggtt gggtgaggga ggagagatgc ccggagagga
     9541 cccagacacg gggaggatcc gctcagagga catcacgtgg tgcagcgccg agaaggaagt
     9601 gctccggaaa gagcatcctt gggcagcaac acagcagaga gcaaggggaa gagggagtgg
     9661 aggaagacgg aacctgaagg aggcggcagg gaaggatctg ggccagccgt agaggtgacc
     9721 caggccacaa gctgcagaca gaaagcggca caggcccagg ggagagaatg ctggtcagag
     9781 aaagcaggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     9841 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9901 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9961 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
    10021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    10081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10201 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10381 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    10441 ttag
//
