LOCUS       sequence-21784-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Cai Y, Jin J, Florens L, Swanson SK, Kusch T, Li B, Workman JL, 
            Washburn MP, Conaway RC, Conaway JW
  TITLE     The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 
            histone acetyltransferase and SRCAP complexes.
  JOURNAL   J Biol Chem. 2005 Apr 8. 280(14):13665-70.
  PUBMED    15647280
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Apr 8. 280(14):13665-70."
COMMENT     Sequence Label: FLAG-H2AZ
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(198..209)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(547..564)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    553..760
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcggat
      121 ccatggctgg cggtaaggct ggaaaggact ccggaaaggc caagacaaag gcggtttccc
      181 gctcgcagag agccggcttg cagttcccag tgggccgtat tcatcgacac ctaaaatcta
      241 ggacgaccag tcatggacgt gtgggcgcga ctgccgctgt gtacagcgca gccatcctgg
      301 agtacctcac cgcagaggta cttgaactgg caggaaatgc atcaaaagac ttaaaggtaa
      361 agcgtattac cactcgtcac ttgcaacttg ctattcgtgg agatgaagaa ttggattctc
      421 tcatcaaggc tacaattgct ggtggtggtg tcattccaca catccacaaa tctctgattg
      481 ggaagaaagg acaacagaag actgtctaac tcgagtctag agggcccgtt taaacccgct
      541 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      601 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      661 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
      721 agggggagga ttgggaagac aatagcaggc atgctgggga
//
