LOCUS       sequence-21786-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Jin J, Cai Y, Yao T, Gottschalk AJ, Florens L, Swanson SK, Gutierrez
            JL, Coleman MK, Workman JL, Mushegian A, Washburn MP, Conaway RC, 
            Conaway JW
  TITLE     A mammalian chromatin remodeling complex with similarities to the 
            yeast INO80 complex.
  JOURNAL   J Biol Chem. 2005 Dec 16. 280(50):41207-12.
  PUBMED    16230350
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Dec 16. 280(50):41207-12."
COMMENT     Sequence Label: FLAG-hIes2
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagca tgccagacta caaggacgat gatgacaagc
      121 tcgagatgga ggcccctgag ccgggagaag ccctggagtt gagcctggcg ggtgcccatg
      181 gccatggagt gcacaagaaa aaacacaaga agcacaagaa gaaacacaag aagaaacacc
      241 atcaggaaga agacgccggg cccacgcagc cgtcccctgc caagcctcag ctcaaactca
      301 aaatcaagct tgggggacaa gtcctgggga ccaagagtgt tcctaccttc actgtgatcc
      361 cagaggggcc tcgctcaccc tctcccctta tggttgtgga taatgaagag gaacctatgg
      421 aaggagtccc ccttgagcag taccgtgcct ggctggatga agacagtaat ctctctccct
      481 ctccacttcg ggacctatca ggagggttag ggggtcagga ggaagaggag gaacagaggt
      541 ggctggatgc cctggagaag ggggagctgg atgacaatgg agacctcaag aaggagatca
      601 atgagcggct gcttactgct cgacagcgag ctctgctcca gaaggcgcgg agtcaacctt
      661 cccctatgct gccgctgcct gtagctgagg gctgcccacc tcccgccctc acagaggaga
      721 tgctgctgaa gcgcgaggag cgggcgcgga agcggcggct ccaggcggcg cggcgggcag
      781 aagagcacaa gaaccagact atcgagcgcc tcaccaagac tgcggcgacc agtgggcggg
      841 gaggccgggg gggcgcacgg ggcgagcggc
//
