LOCUS       sequence-217864-       16456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 16456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-GCaMP3
FEATURES             Location/Qualifiers
     source          1..16456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        233..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        613..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     767..787
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     861..880
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        861..879
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4267..4276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4273..4281,4282..4299,4300..4302,4303..4335,4336..4338,
                     4339..4362,4363..4389,4390..4446,4447..4452,4453..4722,
                     4723..4740,4741..5175,5176..5178,5179..5181,5182..5625)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(4273..4281,4282..4299,4300..4302,4303..4335,4336..4338,
                     4339..4362,4363..4389,4390..4446,4447..4452,4453..4722,
                     4723..4740,4741..5175,5176..5178,5179..5181,5182..5625)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4369..4378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4659..4680
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4786..4807)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5047..5066)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7353..7364
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12462..12479)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12468..12692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12738..13166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12825..12844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13035..13056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13175..13195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13180..13509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13360..13495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13422..13441
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13557..13604
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13623..13997
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    14127..14248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14164..14183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14218..14237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14297..14313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14297..14313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14310..14332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14321..14337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14345..14351,14352..14369,14370..14375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14390..14411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14528..14545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14699..15287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14779..14798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15458..16249,16250..16318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16081..16100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16319..16423)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      241 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      601 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      781 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
      841 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagatt
      901 aagcgtctga tgagtccgtg aggacgaaac ccggagtccc gggtcacgct taacaaccag
      961 atcaaagaaa aaacagacat tgtcaattgc aaagcaaaaa tgtaacaccc ctacaatgga
     1021 tgccgacaag attgtattca aagtcaataa tcaggtggtc tctttgaagc ctgagattat
     1081 cgtggatcaa tatgagtaca agtaccctgc catcaaagat ttgaaaaagc cctgtataac
     1141 cctaggaaag gctcccgatt taaataaagc atacaagtca gttttgtcag gcatgagcgc
     1201 cgccaaactt aatcctgacg atgtatgttc ctatttggca gcggcaatgc agttttttga
     1261 ggggacatgt ccggaagact ggaccagcta tggaattgtg attgcacgaa aaggagataa
     1321 gatcacccca ggttctctgg tggagataaa acgtactgat gtagaaggga attgggctct
     1381 gacaggaggc atggaactga caagagaccc cactgtccct gagcatgcgt ccttagtcgg
     1441 tcttctcttg agtctgtata ggttgagcaa aatatccggg caaaacactg gtaactataa
     1501 gacaaacatt gcagacagga tagagcagat ttttgagaca gccccttttg ttaaaatcgt
     1561 ggaacaccat actctaatga caactcacaa aatgtgtgct aattggagta ctataccaaa
     1621 cttcagattt ttggccggaa cctatgacat gtttttctcc cggattgagc atctatattc
     1681 agcaatcaga gtgggcacag ttgtcactgc ttatgaagac tgttcaggac tggtatcatt
     1741 tactgggttc ataaaacaaa tcaatctcac cgctagagag gcaatactat atttcttcca
     1801 caagaacttt gaggaagaga taagaagaat gtttgagcca gggcaggaga cagctgttcc
     1861 tcactcttat ttcatccact tccgttcact aggcttgagt gggaaatctc cttattcatc
     1921 aaatgctgtt ggtcacgtgt tcaatctcat tcactttgta ggatgctata tgggtcaagt
     1981 cagatcccta aatgcaacgg ttattgctgc atgtgctcct catgaaatgt ctgttctagg
     2041 gggctatctg ggagaggaat tcttcgggaa agggacattt gaaagaagat tcttcagaga
     2101 tgagaaagaa cttcaagaat acgaggcggc tgaactgaca aagactgacg tagcactggc
     2161 agatgatgga actgtcaact ctgacgacga ggactacttt tcaggtgaaa ccagaagtcc
     2221 ggaggctgtt tatactcgaa tcatgatgaa tggaggtcga ctaaagagat ctcacatacg
     2281 gagatatgtc tcagtcagtt ccaatcatca agcccgtcca aactcattcg ccgagtttct
     2341 aaacaagaca tattcgagtg actcataaga agttgaataa caaaatgccg gaaatctacg
     2401 gattgtgtat atccatcatg aaaaaaacta acacccctcc tttcgaacca tcccaaacat
     2461 gagcaagatc tttgtcaatc ctagtgctat tagagccggt ctggccgatc ttgagatggc
     2521 tgaagaaact gttgatctga tcaatagaaa tatcgaagac aatcaggctc atctccaagg
     2581 ggaacccata gaggtggaca atctccctga ggatatgggg cgacttcacc tggatgatgg
     2641 aaaatcgccc aaccatggtg agatagccaa ggtgggagaa ggcaagtatc gagaggactt
     2701 tcagatggat gaaggagagg atcctagctt cctgttccag tcatacctgg aaaatgttgg
     2761 agtccaaata gtcagacaaa tgaggtcagg agagagattt ctcaagatat ggtcacagac
     2821 cgtagaagag attatatcct atgtcgcggt caactttccc aaccctccag gaaagtcttc
     2881 agaggataaa tcaacccaga ctactggccg agagctcaag aaggagacaa cacccactcc
     2941 ttctcagaga gaaagccaat catcgaaagc caggatggcg gctcaaattg cttctggccc
     3001 tccagccctt gaatggtcgg ctaccaatga agaggatgat ctatcagtgg aggctgagat
     3061 cgctcaccag attgcagaaa gtttctccaa aaaatataag tttccctctc gatcctcagg
     3121 gatactcttg tataattttg agcaattgaa aatgaacctt gatgatatag ttaaagaggc
     3181 aaaaaatgta ccaggtgtga cccgtttagc ccatgacggg tccaaactcc ccctaagatg
     3241 tgtactggga tgggtcgctt tggccaactc taagaaattc cagttgttag tcgaatccga
     3301 caagctgagt aaaatcatgc aagatgactt gaatcgctat acatcttgct aaccgaacct
     3361 ctcccctcag tccctctaga caataaaatc cgagatgtcc caaagtcaac atgaaaaaaa
     3421 caggcaacac cactgataaa tcgatgaacc tcctacgtaa gatagtgaaa aaccgcaggg
     3481 acgaggacac tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat gacttgtggc
     3541 ttccaccccc tgaatacgtc ccgctgaaag aacttacagg caagaagaac atgaggaact
     3601 tttgtatcaa cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc aggatcctgc
     3661 ggcacattct gaaatcattc gacgagatat attctgggaa tcataggatg atcgggttag
     3721 tcaaagtggt tattggactg gctttgtcag gatctccagt ccctgagggc ctgaactggg
     3781 tatacaaatt gaggagaacc tttatcttcc agtgggctga ttccaggggc cctcttgaag
     3841 gggaggagtt ggaatactct caggagatca cttgggatga tgatactgag ttcgtcggat
     3901 tgcaaataag agtgattgca aaacagtgtc atatccaggg cagagtctgg tgtatcaaca
     3961 tgaacccgag agcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtccgaag
     4021 aggacaaaga ttcctctctg cttctagaat aacgtacgtc agattatatc ccgcaaattt
     4081 atcacttgtt tacctctgga ggagagaaca tatgggctca actccaaccc ttgggagcaa
     4141 tataacaaaa aacatgttat ggtgccatta aaccgctgca tttcatcaaa gtcaagttga
     4201 ttacctttac attttgatcc tcttggatgt gaaaaaaact attaacatcc ctcaaaggac
     4261 ctgcaggcca ccatgggttc tcatcatcat catcatcatg gtatggctag catgactggt
     4321 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc caccatggtc
     4381 gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg
     4441 agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
     4501 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     4561 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
     4621 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4681 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
     4741 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4801 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
     4861 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4921 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4981 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     5041 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5101 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5161 aagctggagt acaacacgcg tgaccaactg actgaagagc agatcgcaga atttaaagag
     5221 gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg
     5281 gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat gatcaatgaa
     5341 gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga
     5401 aaaatgaaag acacagacag tgaagaagaa attagagaag cgttccgtgt gtttgataag
     5461 gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag
     5521 aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
     5581 caggtaaact acgaagagtt tgtacaaatg atgacagcga agtaaccgcg gtagcttttc
     5641 agtcgagaaa aaaacattag atcagaagaa caactggcaa cacttctcaa cctgagactt
     5701 acttcaagat gctcgatcct ggagaggtct atgatgaccc tattgaccca atcgagttag
     5761 aggctgaacc cagaggaacc cccattgtcc ccaacatctt gaggaactct gactacaatc
     5821 tcaactctcc tttgatagaa gatcctgcta gactaatgtt agaatggtta aaaacaggga
     5881 atagacctta tcggatgact ctaacagaca attgctccag gtctttcaga gttttgaaag
     5941 attatttcaa gaaggtagat ttgggttctc tcaaggtggg cggaatggct gcacagtcaa
     6001 tgatttctct ctggttatat ggtgcccact ctgaatccaa caggagccgg agatgtataa
     6061 cagacttggc ccatttctat tccaagtcgt cccccataga gaagctgttg aatctcacgc
     6121 taggaaatag agggctgaga atccccccag agggagtgtt aagttgcctt gagagggttg
     6181 attatgataa tgcatttgga aggtatcttg ccaacacgta ttcctcttac ttgttcttcc
     6241 atgtaatcac cttatacatg aacgccctag actgggatga agaaaagacc atcctagcat
     6301 tatggaaaga tttaacctca gtggacatcg ggaaggactt ggtaaagttc aaagaccaaa
     6361 tatggggact gctgatcgtg acaaaggact ttgtttactc ccaaagttcc aattgtcttt
     6421 ttgacagaaa ctacacactt atgctaaaag atcttttctt gtctcgcttc aactccttaa
     6481 tggtcttgct ctctccccca gagccccgat actcagatga cttgatatct caactatgcc
     6541 agctgtacat tgctggggat caagtcttgt ctatgtgtgg aaactccggc tatgaagtca
     6601 tcaaaatatt ggagccatat gtcgtgaata gtttagtcca gagagcagaa aagtttaggc
     6661 ctctcattca ttccttggga gactttcctg tatttataaa agacaaggta agtcaacttg
     6721 aagagacgtt cggtccctgt gcaagaaggt tctttagggc tctggatcaa ttcgacaaca
     6781 tacatgactt ggtttttgtg tttggctgtt acaggcattg ggggcaccca tatatagatt
     6841 atcgaaaggg tctgtcaaaa ctatatgatc aggttcacct taaaaaaatg atagataagt
     6901 cctaccagga gtgcttagca agcgacctag ccaggaggat ccttagatgg ggttttgata
     6961 agtactccaa gtggtatctg gattcaagat tcctagcccg agaccacccc ttgactcctt
     7021 atatcaaaac ccaaacatgg ccacccaaac atattgtaga cttggtgggg gatacatggc
     7081 acaagctccc gatcacgcag atctttgaga ttcctgaatc aatggatccg tcagaaatat
     7141 tggatgacaa atcacattct ttcaccagaa cgagactagc ttcttggctg tcagaaaacc
     7201 gaggggggcc tgttcctagc gaaaaagtta ttatcacggc cctgtctaag ccgcctgtca
     7261 atccccgaga gtttctgagg tctatagacc tcggaggatt gccagatgaa gacttgataa
     7321 ttggcctcaa gccaaaggaa cgggaattga agattgaagg tcgattcttt gctctaatgt
     7381 catggaatct aagattgtat tttgtcatca ctgaaaaact cttggccaac tacatcttgc
     7441 cactttttga cgcgctgact atgacagaca acctgaacaa ggtgtttaaa aagctgatcg
     7501 acagggtcac cgggcaaggg cttttggact attcaagggt cacatatgca tttcacctgg
     7561 actatgaaaa gtggaacaac catcaaagat tagagtcaac agaggatgta ttttctgtcc
     7621 tagatcaagt gtttggattg aagagagtgt tttctagaac acacgagttt tttcaaaagg
     7681 cctggatcta ttattcagac agatcagacc tcatcgggtt acgggaggat caaatatact
     7741 gcttagatgc gtccaacggc ccaacctgtt ggaatggcca ggatggcggg ctagaaggct
     7801 tacggcagaa gggctggagt ctagtcagct tattgatgat agatagagaa tctcaaatca
     7861 ggaacacaag aaccaaaata ctagctcaag gagacaacca ggttttatgt ccgacataca
     7921 tgttgtcgcc agggctatct caagaggggc tcctctatga attggagaga atatcaagga
     7981 atgcactttc gatatacaga gccgtcgagg aaggggcatc taagctaggg ctgatcatca
     8041 agaaagaaga gaccatgtgt agttatgact tcctcatcta tggaaaaacc cctttgttta
     8101 gaggtaacat attggtgcct gagtccaaaa gatgggccag agtctcttgc gtctctaatg
     8161 accaaatagt caacctcgcc aatataatgt cgacagtgtc caccaatgcg ctaacagtgg
     8221 cacaacactc tcaatctttg atcaaaccga tgagggattt tctgctcatg tcagtacagg
     8281 cagtctttca ctacctgcta tttagcccaa tcttaaaggg aagagtttac aagattctga
     8341 gcgctgaagg ggagagcttt ctcctagcca tgtcaaggat aatctatcta gatccttctt
     8401 tgggagggat atctggaatg tccctcggaa gattccatat acgacagttc tcagaccctg
     8461 tctctgaagg gttatccttc tggagagaga tctggttaag ctcccaagag tcctggattc
     8521 acgcgttgtg tcaagaggct ggaaacccag atcttggaga gagaacactc gagagcttca
     8581 ctcgccttct agaagatccg accaccttaa atatcagagg aggggccagt cctaccattc
     8641 tactcaagga tgcaatcaga aaggctttat atgacgaggt ggacaaggtg gaaaattcag
     8701 agtttcgaga ggcaatcctg ttgtccaaga cccatagaga taattttata ctcttcttaa
     8761 tatctgttga gcctctgttt cctcgatttc tcagtgagct attcagttcg tcttttttgg
     8821 gaatccccga gtcaatcatt ggattgatac aaaactcccg aacgataaga aggcagttta
     8881 gaaagagtct ctcaaaaact ttagaagaat ccttctacaa ctcagagatc cacgggatta
     8941 gtcggatgac ccagacacct cagagggttg ggggggtgtg gccttgctct tcagagaggg
     9001 cagatctact tagggagatc tcttggggaa gaaaagtggt aggcacgaca gttcctcacc
     9061 cttctgagat gttgggatta cttcccaagt cctctatttc ttgcacttgt ggagcaacag
     9121 gaggaggcaa tcctagagtt tctgtatcag tactcccgtc ctttgatcag tcattttttt
     9181 cacgaggccc cctaaaggga tacttgggct cgtccacctc tatgtcgacc cagctattcc
     9241 atgcatggga aaaagtcact aatgttcatg tggtgaagag agctctatcg ttaaaagaat
     9301 ctataaactg gttcattact agagattcca acttggctca agctctaatt aggaacatta
     9361 tgtctctgac aggccctgat ttccctctag aggaggcccc tgtcttcaaa aggacggggt
     9421 cagccttgca taggttcaag tctgccagat acagcgaagg agggtattct tctgtctgcc
     9481 cgaacctcct ctctcatatt tctgttagta cagacaccat gtctgatttg acccaagacg
     9541 ggaagaacta cgatttcatg ttccagccat tgatgcttta tgcacagaca tggacatcag
     9601 agctggtaca gagagacaca aggctaagag actctacgtt tcattggcac ctccgatgca
     9661 acaggtgtgt gagacccatt gacgacgtga ccctggagac ctctcagatc ttcgagtttc
     9721 cggatgtgtc gaaaagaata tccagaatgg tttctggggc tgtgcctcac ttccagaggc
     9781 ttcccgatat ccgtctgaga ccaggagatt ttgaatctct aagcggtaga gaaaagtctc
     9841 accatatcgg atcagctcag gggctcttat actcaatctt agtggcaatt cacgactcag
     9901 gatacaatga tggaaccatc ttccctgtca acatatacgg caaggtttcc cctagagact
     9961 atttgagagg gctcgcaagg ggagtattga taggatcctc gatttgcttc ttgacaagaa
    10021 tgacaaatat caatattaat agacctcttg aattggtctc aggggtaatc tcatatattc
    10081 tcctgaggct agataaccat ccctccttgt acataatgct cagagaaccg tctcttagag
    10141 gagagatatt ttctatccct cagaaaatcc ccgccgctta tccaaccact atgaaagaag
    10201 gcaacagatc aatcttgtgt tatctccaac atgtgctacg ctatgagcga gagataatca
    10261 cggcgtctcc agagaatgac tggctatgga tcttttcaga ctttagaagt gccaaaatga
    10321 cgtacctatc cctcattact taccagtctc atcttctact ccagagggtt gagagaaacc
    10381 tatctaagag tatgagagat aacctgcgac aattgagttc tttgatgagg caggtgctgg
    10441 gcgggcacgg agaagatacc ttagagtcag acgacaacat tcaacgactg ctaaaagact
    10501 ctttacgaag gacaagatgg gtggatcaag aggtgcgcca tgcagctaga accatgactg
    10561 gagattacag ccccaacaag aaggtgtccc gtaaggtagg atgttcagaa tgggtctgct
    10621 ctgctcaaca ggttgcagtc tctacctcag caaacccggc ccctgtctcg gagcttgaca
    10681 taagggccct ctctaagagg ttccagaacc ctttgatctc gggcttgaga gtggttcagt
    10741 gggcaaccgg tgctcattat aagcttaagc ctattctaga tgatctcaat gttttcccat
    10801 ctctctgcct tgtagttggg gacgggtcag gggggatatc aagggcagtc ctcaacatgt
    10861 ttccagatgc caagcttgtg ttcaacagtc ttttagaggt gaatgacctg atggcttccg
    10921 gaacacatcc actgcctcct tcagcaatca tgaggggagg aaatgatatc gtctccagag
    10981 tgatagatct tgactcaatc tgggaaaaac cgtccgactt gagaaacttg gcaacctgga
    11041 aatacttcca gtcagtccaa aagcaggtca acatgtccta tgacctcatt atttgcgatg
    11101 cagaagttac tgacattgca tctatcaacc ggatcaccct gttaatgtcc gattttgcat
    11161 tgtctataga tggaccactc tatttggtct tcaaaactta tgggactatg ctagtaaatc
    11221 caaactacaa ggctattcaa cacctgtcaa gagcgttccc ctcggtcaca gggtttatca
    11281 cccaagtaac ttcgtctttt tcatctgagc tctacctccg attctccaaa cgagggaagt
    11341 ttttcagaga tgctgagtac ttgacctctt ccacccttcg agaaatgagc cttgtgttat
    11401 tcaattgtag cagccccaag agtgagatgc agagagctcg ttccttgaac tatcaggatc
    11461 ttgtgagagg atttcctgaa gaaatcatat caaatcctta caatgagatg atcataactc
    11521 tgattgacag tgatgtagaa tcttttctag tccacaagat ggttgatgat cttgagttac
    11581 agaggggaac tctgtctaaa gtggctatca ttatagccat catgatagtt ttctccaaca
    11641 gagtcttcaa cgtttccaaa cccctaactg acccctcgtt ctatccaccg tctgatccca
    11701 aaatcctgag gcacttcaac atatgttgca gtactatgat gtatctatct actgctttag
    11761 gtgacgtccc tagcttcgca agacttcacg acctgtataa cagacctata acttattact
    11821 tcagaaagca agtcattcga gggaacgttt atctatcttg gagttggtcc aacgacacct
    11881 cagtgttcaa aagggtagcc tgtaattcta gcctgagtct gtcatctcac tggatcaggt
    11941 tgatttacaa gatagtgaag actaccagac tcgttggcag catcaaggat ctatccagag
    12001 aagtggaaag acaccttcat aggtacaaca ggtggatcac cctagaggat atcagatcta
    12061 gatcatccct actagactac agttgcctgt gaaccggata ctcctggaag cctgcccatg
    12121 ctaagactct tgtgtgatgt atcttgaaaa aaacaagatc ctaaatctga acctttggtt
    12181 gtttgattgt ttttctcatt tttgttgttt atttgttaag cgtgggtcgg catggcatct
    12241 ccacctcctc gcggtccgac ctgggcatcc gaaggaggac gcacgtccac tcggatggct
    12301 aagggagaag ggcgaattcc agcacactgg cggccgttac tagtggatcc gagctcggta
    12361 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg
    12421 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
    12481 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
    12541 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
    12601 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
    12661 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
    12721 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    12781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    12841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    12901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    12961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    13021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    13081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    13141 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
    13201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    13261 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    13321 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    13381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    13441 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    13501 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
    13561 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
    13621 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
    13681 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
    13741 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
    13801 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
    13861 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
    13921 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
    13981 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
    14041 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    14101 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    14161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    14221 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    14281 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    14341 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    14401 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    14461 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    14521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    14581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    14641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    14701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    14761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    14821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    14881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    14941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    15001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    15061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    15121 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    15181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    15241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    15301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    15361 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    15421 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    15481 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    15541 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    15601 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    15661 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    15721 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    15781 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    15841 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    15901 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    15961 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    16021 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    16081 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    16141 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    16201 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    16261 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    16321 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    16381 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    16441 tgccacctga cgtcga
//
