LOCUS       sequence-217865-       14001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHCS163.2-2.2
FEATURES             Location/Qualifiers
     source          1..14001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1410..1709
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4342..4562
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4563..5366
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(10148..10168)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10148..10166)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(10505..11093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10585..10604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11264..12055,12056..12124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11887..11906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12125..12229)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12256..13598
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13219..13266)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13639..13657
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13976..13992)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13976..13992)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgggcttga gtgaacagga tgttgaattc aaaaccttgg
      541 atggaactgt tcttagaggt acactatatc ctacaagtaa tagaggtccc gccatagtta
      601 tgtcgccagg attcaattgc gtcaaggaaa tgcttggcct gcctgatgct gcagctcgtt
      661 ttcaatcggc aggctacacc gtgcttcttt acgatccaag aaccacgggt ctttcaggcg
      721 gcgagcctag aaatgacatc gacccaatcg cgcaggtcgg cgactactct gacgccatca
      781 gctacatcag cacgctttcc tctgtgaatc cttcagaggt ctttatatgg ggcatgtcct
      841 tctcggcggc ggtgggcctt tgtgttgccg cccttgaccc aagagccaag ggtatcatcg
      901 cagtgtgccc attgacagac ttcacataca cggaggaaaa gcttcccatg gtgctgaaaa
      961 agtgcgccca ggacagagct agccaggtcg ccggaaacga tccattttat ttgcccatgc
     1021 tggacgctca gggcagaaac cctgcgggct ttggcgtcgg cgtggatagc gagcaatact
     1081 cgaagattgt cgaggctgga aaagagatcg cccccagaca tgtgaacaga acgactgtgc
     1141 agacctacta tagaatggtt ttgtggcagc cattcggttt gtggaggatg atcaaaaatc
     1201 ttccagtgtt attcgttgtg ccagagctgg acagattgag ccctgcagaa agacaattgg
     1261 gtcattggga ctccttagtc tgcccaaaaa gaaaggtctt ggtgccaggc gtgggacata
     1321 tggagataat ggaaggcgac cacgtggatg agataatgac tgacatggag aaattcctga
     1381 gagatgtctt ggagggttca gtaccatagg cggatctctt atgtctttac gatttatagt
     1441 tttcattatc aagtatgcct atattagtat atagcatctt tagatgacag tgttcgaagt
     1501 ttcacgaata aaagataata ttctactttt tgctcccacc gcgtttgcta gcacgagtga
     1561 acaccatccc tcgcctgtga gttgtaccca ttcctctaaa ctgtagacat ggtagcttca
     1621 gcagtgttcg ttatgtacgg catcctccaa caaacagtcg gttatagttt gtcctgctcc
     1681 tctgaatcgt ctccctcgat atttctcatt ttccttcgca tgccagcatt gaaatgatcg
     1741 aagttcaatg atgaaacggt aattcttctg tcatttactc atctcatctc atcaagttat
     1801 ataattctat acggatgtaa tttttcactt ttcgtcttga cgtccaccct ataatttcaa
     1861 ttattgaacc ctcacgatcc agttctccag tgacacagcc tttatctggt caaacctttc
     1921 tttctaatca cctatgctga tgcttaatta agggattttt gtctccatca acggcatgcg
     1981 cccaaaaatg acgttttttt taacccatag acacgaaact acccattttc caccggcctg
     2041 acctaccacc ggaacaacgg ccatctccaa cttgcaagtt ggggaaatta agagcatcgc
     2101 aggtttaatg gaagaaaaaa aaaaggtaca gcacagcgca aatggagtta gttcccttat
     2161 gtcacacact cacacacagt cggtcagatc aagcatactg ggtgcgtata aatagagtgg
     2221 ccattgccac cctgtttatc tcaaaatctg tcttgttagt ggtcttctcc ctttttcagg
     2281 ttacaattct cttgtttcta cttagtatat aagtatatca agctatatta agcatactat
     2341 caactgtcaa ctctatcctc aaaatacaat acaaaatgtt ggccaagcag atcctttcca
     2401 acttgcactt gagtgaagcg ttgtattcag tggtgctagt gctgctagcc ggtacctgcg
     2461 tcttgtatac ggcggttcta gcgatctaca gattgtactt tagcccactg gctccttttc
     2521 ctggacctaa gttggcagct gtcaccgcgt ggtacgagac atactttgag ttggtgaaga
     2581 atggaggtgg tcatttcaca ttcgagatca tgagaatgca tgagaagtac ggacccatcg
     2641 tgagaatcag cccaaaagag cttcatattg ccgacagtga attctatgat acgatttaca
     2701 catcaacgca aggcttcgac aaatctgcac acgttcagga tagattcgga gcgccagagg
     2761 ccgcgttttc aactcctgat catgaagtcc acaagagacg tcgtgctgct atctccccat
     2821 tctttgctaa aagacgtatc catgagcaag cgcccatgat ccaaggccac gtcaacaaga
     2881 tcgtcagaag acttcatcac gaatatgctg gaaaaggaaa gattttgaat ttgaatgacc
     2941 tttattcgag ctatgtgtca gatgtaataa tgagctacgc attcaatcgt tcgtacggtt
     3001 ttttggacga gccagacttt gtctcgtctt tcacatcatc cattcaagga ttgaaagact
     3061 tcgtacatta cgcgcagcag cttccctggt tgccaaagat cattgacaga ttgcctgaca
     3121 gacttgtcgg ctacatccaa cccagcatga aagccatcct acacttccaa aactcgatga
     3181 ggaatcaggt gaaggaggtg agagaaaact tgcactctga caaggccgaa gcccaggaag
     3241 gtaccatctt cttcagcatc atcagatccg aactaccacc cgaagatttg acgatccccc
     3301 gtttgaagga tgaggcgatg agcgttgtcg gcgccggcat cgagacgacc aagatggctt
     3361 tggcagttct gtgctttcac atcatcaaca cccccagcgt gttggaacgt ctgcagaatg
     3421 agctacaaga tgccattccc gatccatcaa accccccacc actgtctgtt ttggagaaac
     3481 tgccatatct atatgcgtgt ctgcaggagg gcattcgtct gtcttacggt gcgatagcac
     3541 gttcgcaaag aatttcaagg cacaacccca tcaagtacaa cgattgggtt atcccacctg
     3601 gtgttatggt gtcgctagat acataccaca tgcaccacga cgaggccgtc ttcccacaaa
     3661 gtcacgaata caggccagag agatggcttg gtaatcccag aggccccgac ggcgagaagg
     3721 ctcttacaaa gtatttgact gcgtttggaa gaggtacaag gatgtgtgtg ggattcaacc
     3781 tggcctatgc tgagatgacg cttgtacttg cggcactgtt cagaagcttc acctttgagt
     3841 tgtttgagac ggacaaaggt gacgtggaga tttttaggga tatgataggc ttggaggtta
     3901 agccaggtag taagggtgtc agggtcaaag tgagtggtaa aggtgaagtt cagccttaaa
     3961 caaatcgctc ttaaatatat acctaaagaa cattaaagct atattataag caaagatacg
     4021 taaattttgc ttatattatt atacacatat catatttcta tatttttaag atttggttat
     4081 ataatgtacg taatgcaaag gaaataaatt ttatacatta ttgaacagcg tccaagtaac
     4141 tacattatgt gcactaatag tttagcgtcg tgaagacttt attgtgtcgc gaaaagtaaa
     4201 aattttaaaa attagagcac cttgaacttg cgaaaaaggt tctcatcaac tgtttaaaag
     4261 gaggatatca ggtcctattt ctgacaaaca atatacaaat ttagtttcaa agatgaatca
     4321 gtgcgcgaag gacataactc attcaattca attcatcatt ttttttttat tctttttttt
     4381 gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
     4441 ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
     4501 attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa
     4561 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
     4621 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     4681 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     4741 cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
     4801 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
     4861 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
     4921 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
     4981 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
     5041 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
     5101 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
     5161 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
     5221 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
     5281 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
     5341 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
     5401 actcacaaat tagagcttca atttaattat atcagttatt accctatgcg gtgtgaaata
     5461 ccgcacagat gcgtaaggag aaaataccgc atcaggatag gaaaaaaccg agcttccttt
     5521 catccggcgc ggctgtgttc tacatatcac tgaagctccg ggtattttaa gttatacaag
     5581 ggaaagatgc cggctagact agcaagtttt aggctgctta acattatgga taggcggata
     5641 aagggcccaa acaggattgt aaagcttaga cgcttctggt tggacaatgg tacgtttgtg
     5701 tattaagtaa ggcttggctg gggatagcaa cattgggcag agtatagaag accacaaaaa
     5761 aaaggtatat aagggcagag aagtctttgt aatgtgtgta acttctcttc catgtgtaat
     5821 cagtatttct acttacttct taaatataca gaagtaagac agataaccaa cagcctttcc
     5881 cagatataca tatatatctt tatttcagct taaacaataa ttatatttgt ttaactcaaa
     5941 aataaaaaaa aaaaaccaaa ctcacgcaac taattattcc ataataaaat aacaacatgg
     6001 acacgttgaa gagattgtgg cctcagttgc ggcccagccc cagagcccca ttgaccgagg
     6061 ccaacctgcc atcgcaagcg ggcagagtcg tgttggtcac gggcgccacc gccggcgtcg
     6121 gatacgagct gtcgagaatc ttgtacaacg cgggcgccag agtctacatg gccgcgagat
     6181 cgcccgccaa agccgaccag gccatggcca gaatcagaag cgcccacccc gcgcccaagg
     6241 tgcccggcgg cgccctggtc ttcttgcact tggactacgc cgacctggcc accgtcaagc
     6301 catgcgcgtt ggcgttcctg tccaaggagc caagactgca cgtcctgttc aacaatgccg
     6361 gcatggcctc cgccccaccc gaagcgagat cgagcgccgg cttggagctg cacatgcagg
     6421 tcaactgcgt gggcggcttt ttgctgacca agttgttgac gcctgcgttg gaggctgctg
     6481 ccaaagacga cgacgcaccc ccagccgccg tgagagtcgt cttcagcagc agcatgttgg
     6541 tcgacgccaa ggcccccgcc gacggcgtcc cacccgacga gttggacaac ccctcgttgg
     6601 acggcgacag aaactacgcc atcagcaagg ccgccaactg gttcttggcc gtgcagttcg
     6661 cccagagatt gggtccccag ggcgtcgtgg tcttgaccca gaacccaggc aacctgagaa
     6721 ccgccatctt cgacgaggtg cccaaggcca ttgtctgggc gagctaccca gtgctgttca
     6781 agtcgattga cggcgcccac acgtcgctgt ggtcgggctt ctcgagcgac gtcaccctgg
     6841 cagatagcgg cagatacgtc gtgccctggg gcaagtggca tccaaaccac cgtccagagc
     6901 agctgttggc catcaaggac aaaagcgagg gcggcaaggg ctacgcccaa catctatttg
     6961 agtggtgcga cgcgaagacc aaggcgttcc agtagagtgc ttttaactaa gaattattag
     7021 tcttttctgc ttattttttc atcatagttt agaacacttt atattaacga atagtttatg
     7081 aatctattta ggtttaaaaa ttgatacagt tttataagtt actttttcaa agactcgtgc
     7141 tgtctattgc ataatgcact ggaaggggaa aaaaaaggtg cacacgcgtg gctttttctt
     7201 gaatttgcag tttgaaaaat aactacatgg atgataagaa aacatggagt acagtcactt
     7261 tgagaacctt caatcagctg gtaacgtctt cgttaattgg atactcaaaa aagatggata
     7321 gcatgaatca caagatggaa ggaaatgcgg gccacgacca cagtgatatg catatgggag
     7381 atggagatga tacctccatt gggccgatga agttagtcga cggatagaag cggttgtccc
     7441 ctttcccggc gagccggcag tcgggccgag gttcggataa attttgtatt gtgttttgat
     7501 tctgtcatga gtattactta tgttctcttt aggtaacccc aggttaatca atcacagttt
     7561 cataccggct agtattcaaa ttatgacttt tcttctgcag tgtcagcctt acgacgatta
     7621 tctatgagct ttgaatatag tttgccgtga ttcgtatctt taattggata ataaaatgcg
     7681 aaggatcgat gacccttatt attatttttc tacactggct accgatttaa ctcatcttct
     7741 tgaaagtata taagtaacag taaaatatac cgtacttctg ctaatgttat ttgtccctta
     7801 tttttctttt cttgtcttat gctatagtac ctaagaataa cgactattgt tttgaactaa
     7861 acaaagtagt aaaagcacat aaaagaatta agaaaatgca acctgcttca ttgttgcttg
     7921 ccctggctgc tgccgctgct gcgtcgccaa cctacggctc gttccccgtc agaggacaga
     7981 cgtgtagaaa gttgccccag gacaaggact ggccatcgcc acagatctgg aacgtcctga
     8041 acgcgaccgt gggaggacgt ctgattgcca caacacccct agcatctccc tgccacgacc
     8101 ccaactatga tgccgcaaag tgtgacgctt tgagagcggg ctgggtgtac ccacagacgc
     8161 atttcgattc ctccacctcc attgttgctc catacttcca gaatcagagc tgcgacccat
     8221 ttacgtctag agcaagcact tgcaaggttg gaacgtaccc cagctacgcc atcaaggtca
     8281 ccaaggcctc ggatgccata gcaggcatgc ttttcgcaca attgaacaat gtgcgtctgg
     8341 tcatcaagaa caccggtcac gacttgttgg gccgttctac tggtaccgga ggtctttcgc
     8401 tgtggacgca ctacttgaag gacatcacca tcagtgatta caagtcgtct tcttacaccg
     8461 gcaaggctat gaagatgggt gctggtgtcc aagcctacga agcctacgag gccgctcaca
     8521 agcagagcct gagagttgtg ggtggtactt gcccaactgt tggtttggcc ggtggctaca
     8581 cccagggtgg tggccactcg atgctttcta ccgtccacgg tcttggagct gaccaggtcc
     8641 tggaatggga ggttgtcacc gccaacggca gcctgttgac cgccaccccc gacaagaaca
     8701 gcgatctgta ctgggccctg tccggtggcg gcggcggtac ctacggcctt gtcttgagca
     8761 tgacggtcaa ggcacacgcc gacaccaagg tcaccggcgc catgttgacc atcatccaac
     8821 agggcgccga ggacgccttc tggaagggca tcacgttgtt ccacgcctcc atgacctcgt
     8881 acacggacgc cggcgcggcc tttggctatt acttgggcca gggcatcatg gccatccaac
     8941 ccgtcacctt cccaggcaag tccaaggccg aggtcgaggc gttgatgttg gacctgacca
     9001 agaggttgga tgccgccggt actgtgtact cgttgaccgt caccgagttc agcaactact
     9061 acgacttctt caacaactac tacggcccac ttccctacgg cgtctacacc tcggcccagg
     9121 tccagggtgg acgtctggtc ccaagaacag tcgtcgccac cgccgcctcc aaccaggcct
     9181 tcaccgacgc cttgaaggcc atcgtccagg accaggactt ccacgtcgtc ggcgtcggct
     9241 tggacgtcag caagacagca accgcttcca acgccgtgca ccccaagtgg agatcgaccg
     9301 ccatcaccat cgtcgtcgcc gccaactggg actacacgca gccatttgcg accaacgccg
     9361 ccaacgaaac cttgatcacc gacaagtacg acccattgtt gacggccgtc acgggcgccg
     9421 acagcggcgc ctacgtcaac gagggcgact tccagcagcc cgcgttccag gacaactttt
     9481 tcggtgcaaa ctacgccaag ctgagatcca tcaagaagaa gtacgacccc aacagcttgt
     9541 tctacgcgac caagctggtg ggctcggaag actgggctgt gcaggctgat ggcaggcttt
     9601 gcaaggcctg aggagattga taagactttt ctagttgcat atcttttata tttaaatctt
     9661 atctattagt taattttttg taatttatcc ttatatatag tctggttatt ctaaaatatc
     9721 atttcagtat ctaaaaattc ccctcttttt tcagttatat cttaacaggc gacagtccaa
     9781 atgttgattt atcccagtcc gattcatcag ggttgtgaag cattttgtca atggtcgaaa
     9841 tcacatcagt aatagtgcct cttacttgcc tcatagaatt tctttctctt aacgtcaccg
     9901 tttggtcttt tatagtttcg aaatctatgg tgataccaaa tggtgttccc aattcatcgt
     9961 tacgggcgta ttttttacca attgaagtat tggaatcgtc aattttaaag tatatctctc
    10021 ttttacgtaa agcctgcgag atcctcttaa gtatagcggg gaagccatcg ttattcgata
    10081 ttgtcgtaac aaatactttg atcggcgcta tgcggccgcc accgcggtgg agctccagct
    10141 tttgttccct ttagtgaggg ttaattgcgc gcttggcgag actcaaacaa gatatatttt
    10201 gcccgcctta cagacgaaac tagttggagg ttatggagca tactatcacg tgggcggcca
    10261 ctggtgagtt actacacccc aggggcaacg ttgatgctcc taaaaaactc tggctggacg
    10321 caagccgtaa cacccgtgtc acttcataat cgtttgcaat tcagggcttg atctacacat
    10381 taccctgtta tccctattaa ttaatacggt tatccacaga atcaggggat aacgcaggaa
    10441 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10501 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10561 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10621 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10681 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10741 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10801 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    10861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    10921 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
    10981 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11041 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11101 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11161 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11221 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11281 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11341 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11401 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11461 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11521 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11581 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11641 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11701 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11761 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    11821 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    11881 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11941 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12001 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12061 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12121 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    12181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    12241 gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
    12301 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
    12361 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
    12421 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
    12481 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
    12541 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
    12601 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
    12661 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
    12721 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
    12781 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
    12841 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
    12901 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
    12961 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
    13021 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
    13081 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
    13141 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
    13201 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
    13261 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
    13321 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
    13381 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
    13441 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
    13501 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
    13561 tcatccttca atgctatcat ttcctttgat attggatcat actaagaaac cattattatc
    13621 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    13681 gatgacggtg aaaacctcgg cgcgccatta ccctgttatc cctaatatac aacgcaaaga
    13741 agctggaaaa ttggcgaggg aatcctgttt ctgtctatcc aagaatgggc atgaggtggc
    13801 aaccgtcgtg ctagcgtaca gggtgcactt tgtaaccatt tgggacaccg gacactcgct
    13861 gttttcgaaa ttacccttta agcgcgggta ttgaaccagg cttatgccca agatcgtagc
    13921 aagcatttaa atgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
    13981 actagttcta gagttaatta a
//
