LOCUS       sequence-217866-       14586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU11.1-2.2
FEATURES             Location/Qualifiers
     source          1..14586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1506..1805
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4462..4682
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4683..5486
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(10733..10753)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10733..10751)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(11090..11678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11170..11189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11849..12640,12641..12709))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12472..12491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12710..12814)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12841..14183
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13804..13851)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     14224..14242
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14561..14577)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14561..14577)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgttttcag aatcccatct caagatgtct cgtaccttca
      541 cccagttctg tttcccttct gtctcgccat cctcattggt aattgaagtc gacttcttgt
      601 cgtctttcgc tttgccagtg ctacgtttca ttgtcaaact cccttcatac gccttgtacc
      661 atctatggac gctattttta ttcacgaagt cagacattaa gacaactgta atacctataa
      721 cttctctggc cgtcgcgtcc gctccacttg gcgatatctc acatcttcca catgttatat
      781 tctggatttg gtttcatgtc ctacagttcg acgtctcaaa ccagacgctg aaaccagaag
      841 aagacgagta caacaagcga gaccgtcctc tgcccgcaaa gcgcatcact tggcgcaatg
      901 cattgatttt gcgttgggtt ctcgtcccgg cgtgttggat actctcatcc ttttacagtg
      961 ttgaaactgt gtatgcgagt atcgcactgt gcattctgac atatgtatac gacgaaatgg
     1021 gttatgctgc tggtcactgg ttcggtcgca atttcgtgaa tgccttaggc ttcatgtcat
     1081 tcgaagttgg agcttgtctc attgctggtt cgaatcgaca tgcgcttgat catataagtt
     1141 ggctatctgt actatgcagt gcagggatct ttgcaacgac gatccaggcc caggacttca
     1201 aggacactga aggtgatcga cttgtgggcc ggaaaacctt acccattgtt gcgccgacag
     1261 ttgctcgtcc tactcttctg ctcgcgcttt tggcgtggtc tatcggatta agcacgcttt
     1321 ggcagctcag cactactgta gcccttgctt tcaatgtcct agccatagca gttggagcac
     1381 gtttcgtttc acttaagtcc atcaaggcag atcagcgcag tttttatctc tacaacgttt
     1441 ggttatccgt cgcacacgca ttaccagcgt atttccgttt tatcgctcag gcaccagtgt
     1501 tctgagcgga tctcttatgt ctttacgatt tatagttttc attatcaagt atgcctatat
     1561 tagtatatag catctttaga tgacagtgtt cgaagtttca cgaataaaag ataatattct
     1621 actttttgct cccaccgcgt ttgctagcac gagtgaacac catccctcgc ctgtgagttg
     1681 tacccattcc tctaaactgt agacatggta gcttcagcag tgttcgttat gtacggcatc
     1741 ctccaacaaa cagtcggtta tagtttgtcc tgctcctctg aatcgtctcc ctcgatattt
     1801 ctcattttcc ttcgcatgcc agcattgaaa tgatcgaagt tcaatgatga aacggtaatt
     1861 cttctgtcat ttactcatct catctcatca agttatataa ttctatacgg atgtaatttt
     1921 tcacttttcg tcttgacgtc caccctataa tttcaattat tgaaccctca cgatccagtt
     1981 ctccagtgac acagccttta tctggtcaaa cctttctttc taatcaccta tgctgatgct
     2041 taattaaggg atttttgtct ccatcaacgg catgcgccca aaaatgacgt tttttttaac
     2101 ccatagacac gaaactaccc attttccacc ggcctgacct accaccggaa caacggccat
     2161 ctccaacttg caagttgggg aaattaagag catcgcaggt ttaatggaag aaaaaaaaaa
     2221 ggtacagcac agcgcaaatg gagttagttc ccttatgtca cacactcaca cacagtcggt
     2281 cagatcaagc atactgggtg cgtataaata gagtggccat tgccaccctg tttatctcaa
     2341 aatctgtctt gttagtggtc ttctcccttt ttcaggttac aattctcttg tttctactta
     2401 gtatataagt atatcaagct atattaagca tactatcaac tgtcaactct atcctcaaaa
     2461 tacaatacaa aatggatccc gtactgcgat ggatgacatc tgaacagacg tttacccctg
     2521 ttatcggcct cgttgggctc ttctgtagcc tcggtcttgg attatacgct agtaaacgag
     2581 cctatacccc aagcaaacaa agtctaggac atggtcttcc gttgcctcca ggaccaaaat
     2641 cactccccat ccttggcaat gctcttgata tgcccaacaa gcgtgaatgg gagacattca
     2701 caaaatgggc agacgaatac ggtgatattg tccatatcaa ggtgtttgga cgtgatatta
     2761 tctacattag ctctgccaaa gttgcgagcg acctgtttga gaaaagatct gctatttatt
     2821 ccgacaggcc acgtttgcct ttacttgcag agctgatgga ctttgagtgg aacttcgggt
     2881 tcatgcatta tggagacgaa tggcgaaaga atagacgaat gtttgtgagc aagttcggtc
     2941 cgacgaaggt tcatactttc aatccagtcc aagaacgtgc ttctggtctt ctgctgggac
     3001 gacttttgga gactcccgag gacttcattg accacctccg tcttcatgct ggtcaattga
     3061 taatgatgtc cgtttacggt atatttgtcg actcaaggca gcacccgtat ataacgaccg
     3121 ctcaagcagt gatggactcc gtttccgatg cggcacgttc tggtgcatat ctcgttgatg
     3181 cgttcccaat actgaaaaga ttaccaacat ggctacctgg cacacattac caacgcattg
     3241 cacgtagatg ggcttccgat gtacgaaagc tcaggtatat tcctttcctt gcgagcaaga
     3301 ctaaattgga agatggtgca ccgccctatc caacctttgt tgctgatatg ctagaggagt
     3361 acggttacga tgaaaataac gctgagaata tgatccggga ctgtgcagct ctcgcgtatg
     3421 gagcggggtc ggacacgtct gtctccactt tatcggcatt tatcctcgca atggttcttt
     3481 acccggacgt tcaaaggaga ggtcaagacg agttgaaggc tgtcattggt tcgtctagac
     3541 tccccacgtt cgctgaccga aaggatctac catatatcat ggcaattgcg aaggagacga
     3601 ttcgctggca cacagttgct ccacaggctc ttccacatgt tctcagagaa gatgatgtct
     3661 acaatggtta ttatcttcca gcggggtcta ttgttattgg aaatacatgg aaaattgcgc
     3721 atgaccctac agtctatcca gatccaatga acttcaagcc agaacgctac ttcaattcag
     3781 agggtgaact cgactacgcc tccatagatc cagcaaagtt tgcatttggt tacggcagaa
     3841 gaatctgtgc aggataccat tacgctgaga acacgctgtg gattacaatc gctttcattt
     3901 tgcaggtttt cagtattaca aatgcaaaag acaaggccgg taatcctatc cctgttactc
     3961 taaacgcttc ctcgggtgtc atatcccatc ccgagccatt cccgtgttcg attatgccac
     4021 gctctacaga agcggaaacc ataattcgcc gagccgctgc caatgccaat tttgcataaa
     4081 caaatcgctc ttaaatatat acctaaagaa cattaaagct atattataag caaagatacg
     4141 taaattttgc ttatattatt atacacatat catatttcta tatttttaag atttggttat
     4201 ataatgtacg taatgcaaag gaaataaatt ttatacatta ttgaacagcg tccaagtaac
     4261 tacattatgt gcactaatag tttagcgtcg tgaagacttt attgtgtcgc gaaaagtaaa
     4321 aattttaaaa attagagcac cttgaacttg cgaaaaaggt tctcatcaac tgtttaaaag
     4381 gaggatatca ggtcctattt ctgacaaaca atatacaaat ttagtttcaa agatgaatca
     4441 gtgcgcgaag gacataactc attcaattca attcatcatt ttttttttat tctttttttt
     4501 gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
     4561 ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
     4621 attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa
     4681 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
     4741 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     4801 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     4861 cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
     4921 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
     4981 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
     5041 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
     5101 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
     5161 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
     5221 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
     5281 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
     5341 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
     5401 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
     5461 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
     5521 actcacaaat tagagcttca atttaattat atcagttatt accctatgcg gtgtgaaata
     5581 ccgcacagat gcgtaaggag aaaataccgc atcaggatag gaaaaaaccg agcttccttt
     5641 catccggcgc ggctgtgttc tacatatcac tgaagctccg ggtattttaa gttatacaag
     5701 ggaaagatgc cggctagact agcaagtttt aggctgctta acattatgga taggcggata
     5761 aagggcccaa acaggattgt aaagcttaga cgcttctggt tggacaatgg tacgtttgtg
     5821 tattaagtaa ggcttggctg gggatagcaa cattgggcag agtatagaag accacaaaaa
     5881 aaaggtatat aagggcagag aagtctttgt aatgtgtgta acttctcttc catgtgtaat
     5941 cagtatttct acttacttct taaatataca gaagtaagac agataaccaa cagcctttcc
     6001 cagatataca tatatatctt tatttcagct taaacaataa ttatatttgt ttaactcaaa
     6061 aataaaaaaa aaaaaccaaa ctcacgcaac taattattcc ataataaaat aacaacatgg
     6121 caccaatcat aggtgccgca ggaattgctt gcaccctatg tttgggtctt ttcgtgtatc
     6181 ttagcaagcg agctcaagca cccagcaaac agagcagagg acatggtctt ccgcttccgc
     6241 ctgggcctaa gccactgccg tttcttggga atgcacttga tatgcctgtg aaacgtgaat
     6301 gggaggcatt tgccaaatgg gcgaatgaat atggtgatat tgttcatgtc agcgtcttcg
     6361 gccagaactt tattttcctc aattctgcca aagttgtgaa cgacctgttc gacaagaggg
     6421 gttctatcta ttctgataaa cctcgcttac cattactcac agaattacta gactttgaat
     6481 ggaacttcgg ttttatgccc tatggagagg agtggagaaa gtatagacgc atgtttgtgg
     6541 gcaagttcgg agctagcgct gctcatatct tcaatccaat ccatgagcgt gtctcgggcc
     6601 tattgctaca gcggtttttg gatactccag aggacttcat agatcatctt cgcctccatg
     6661 cgggtcagat tatcatgatg tcgacatacg gtatttttgt agattcaaga cagcatccat
     6721 atattcaaat tgcccaagcc gtgatggact cagtttctga tgctgcacga gctggggcat
     6781 acctggtaga cgcgtttccg atcctgaaga aagtaccaac gtggttccct ggagcgcatt
     6841 atcagcgtgt tgctcagggc tgggctgcgg aagttcgaag gcttaggttc attccttttc
     6901 ttgcgagcaa gaaccaattg gccgacggca aagcgccacc ttttccgtcc tttgtcgctg
     6961 atgagctaga ggaaaacggc tatgacgaga aagacgctga actgatgatt caagattgtg
     7021 cggctgtcgc atacggagcg ggatccgaca cttccgtcgc aacgctcact gcattcgttc
     7081 tcgcaatggt tattcacccg gaaattcaaa agaaagctca ggaagagctt gcagcagtcg
     7141 ttggaacttc gaggctccct acgttcgctg atcgcaatga tctcccatat atttcagcca
     7201 tcatgaagga gacaatacga tggcatacag tagctccgca ggcactcccg cataccctca
     7261 gagaagatga cgtatataat ggttatcata tcccagcagg atccattgtt atcggcaata
     7321 cttggaatat tacgcatgac ccggcactct acccagaccc tatggagttc aagccggaac
     7381 gttttattaa ttcagaaggc aaactcgatt tttccggcgc cacggacccg tcaaagttcg
     7441 cttttggtta tggcagaagg atttgcgccg gatggcactt cgctgagaat acgatattca
     7501 ttacaatagc actcgttttg cactgtttca acatcacgaa tgcaaaggat aaggctggga
     7561 tgcccattcc tgtctctctt aatgctggtt ctggtgtcat ttcgcatcct gagccgttcc
     7621 catgttccat cactccacgc tctgcccagg cggaagcctt aattcgtcaa gctgccgcct
     7681 ccgcccaatt tgtctagagt gcttttaact aagaattatt agtcttttct gcttattttt
     7741 tcatcatagt ttagaacact ttatattaac gaatagttta tgaatctatt taggtttaaa
     7801 aattgataca gttttataag ttactttttc aaagactcgt gctgtctatt gcataatgca
     7861 ctggaagggg aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc agtttgaaaa
     7921 ataactacat ggatgataag aaaacatgga gtacagtcac tttgagaacc ttcaatcagc
     7981 tggtaacgtc ttcgttaatt ggatactcaa aaaagatgga tagcatgaat cacaagatgg
     8041 aaggaaatgc gggccacgac cacagtgata tgcatatggg agatggagat gatacctcca
     8101 ttgggccgat gaagttagtc gacggataga agcggttgtc ccctttcccg gcgagccggc
     8161 agtcgggccg aggttcggat aaattttgta ttgtgttttg attctgtcat gagtattact
     8221 tatgttctct ttaggtaacc ccaggttaat caatcacagt ttcataccgg ctagtattca
     8281 aattatgact tttcttctgc agtgtcagcc ttacgacgat tatctatgag ctttgaatat
     8341 agtttgccgt gattcgtatc tttaattgga taataaaatg cgaaggatcg atgaccctta
     8401 ttattatttt tctacactgg ctaccgattt aactcatctt cttgaaagta tataagtaac
     8461 agtaaaatat accgtacttc tgctaatgtt atttgtccct tatttttctt ttcttgtctt
     8521 atgctatagt acctaagaat aacgactatt gttttgaact aaacaaagta gtaaaagcac
     8581 ataaaagaat taagaaaatg gcagcgtaca ctggtccatt acgctctcgc gcgatgtcct
     8641 tccctacgaa gccagtcctt aaagatcttt ttcgtcgacg tgcttccgtg tctcttgatt
     8701 ttctcgtcgt tggaggcagt atcgcaggtc tcgcctgtgc gtacaatcta aaacaagctg
     8761 ggcataatat tgagacgttt tgccttgcat gtgcgctatg tcctcgtgta ttgattcaac
     8821 aggtatctgg aggcatccga gttccgccaa atatgacgaa acttcttatg cattggggat
     8881 tgggtgacag attgatcaag actggtgtgc ggtgtcccta catagagttc ctcgaaagcg
     8941 ataccggatc gttccttagt tctctggtgt ttcacgaaga agtgatgaga gagcttcaag
     9001 ctgacatgta tggattgaca gtgagtacaa aaatagctgg cgatcatctc gacacggcac
     9061 tcatccgacg ccccgatcaa cagtattcgg atctttattc aatgatatat gacatcacgg
     9121 ttgcatcggg agttaatgta gagtacaacg gcgaggtggt agatgtggac gtggacaagc
     9181 caagtgttac attggcatca gggcggaagt tacgtgcgga cattattgtc ggtgcagatg
     9241 gagcaacaag tatagtgcgc gagaaattgg ttggacgtaa agaaaaccat agaccagggc
     9301 cgttaaacgg ctatacatat actgtccctg tcagtaagat gatggaagac cccgaactta
     9361 ggccactagc ggagaacaag ggagtgtgga tagtttggat gggaaataat acctgcgtct
     9421 tgacattttt ggttgtaagt ttatcaactt tatcggagat gaagattgac atacacacat
     9481 ctctaaagcg accagacgag tatgcgattc aactgattca tcccactaag aatgccaggc
     9541 atgactggta taagctaacg aatatggcgg caattgtggg taaatttgat gactatgagc
     9601 caagagtgag acgtcttttg cggcttgcaa cgcaaggtgt cgaaacacgg catatcatac
     9661 aagaaccgct ggaagattgg tacagtgaaa atggccatgt ggtagtcctt ggggatgcag
     9721 cccatgtatt gaaccctgga acgagccatg gttcttcact ggctgtggaa gatgccgcag
     9781 ttctcggttc tctcttctct cggttgcgta cacgtagctc acgcgaaatt ctgcgattgc
     9841 tggctgcgta ccaagaaatt cgagaggacc gctgtatcga agtcacgaga accgagtacg
     9901 aaacttgtgc attttcagtt ctgcccaggg gggatcctag acgcgaagag cgagatcttg
     9961 gatttgctgc ctcacgggat atgaaggttc tcgattggga aaatctcgac gacccgttcc
    10021 tacaacgcat ttgggagcaa ttcaagggtt ctttcggcta cgaggcgtac gatgccgcgg
    10081 atgattggtg ggtagattgg ggtattatgc aagaacggat ggctgcatca tcaatggctg
    10141 ttatcgagag ctacgagtcc tcgcagaacg gcgaaggcac ggatctatac aactaaggag
    10201 attgataaga cttttctagt tgcatatctt ttatatttaa atcttatcta ttagttaatt
    10261 ttttgtaatt tatccttata tatagtctgg ttattctaaa atatcatttc agtatctaaa
    10321 aattcccctc ttttttcagt tatatcttaa caggcgacag tccaaatgtt gatttatccc
    10381 agtccgattc atcagggttg tgaagcattt tgtcaatggt cgaaatcaca tcagtaatag
    10441 tgcctcttac ttgcctcata gaatttcttt ctcttaacgt caccgtttgg tcttttatag
    10501 tttcgaaatc tatggtgata ccaaatggtg ttcccaattc atcgttacgg gcgtattttt
    10561 taccaattga agtattggaa tcgtcaattt taaagtatat ctctctttta cgtaaagcct
    10621 gcgagatcct cttaagtata gcggggaagc catcgttatt cgatattgtc gtaacaaata
    10681 ctttgatcgg cgctatgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
    10741 gagggttaat tgcgcgcttg gcgagactca aacaagatat attttgcccg ccttacagac
    10801 gaaactagtt ggaggttatg gagcatacta tcacgtgggc ggccactggt gagttactac
    10861 accccagggg caacgttgat gctcctaaaa aactctggct ggacgcaagc cgtaacaccc
    10921 gtgtcacttc ataatcgttt gcaattcagg gcttgatcta cacattaccc tgttatccct
    10981 attaattaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    11041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    11101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    11161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11521 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    12001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    12061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    12121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    12181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    12241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    12301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    12361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    12421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    12481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    12601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    12661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12841 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
    12901 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
    12961 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
    13021 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
    13081 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
    13141 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
    13201 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
    13261 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
    13321 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
    13381 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
    13441 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
    13501 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
    13561 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
    13621 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
    13681 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
    13741 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
    13801 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
    13861 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
    13921 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
    13981 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
    14041 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
    14101 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
    14161 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat
    14221 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    14281 ctcggcgcgc cattaccctg ttatccctaa tatacaacgc aaagaagctg gaaaattggc
    14341 gagggaatcc tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc
    14401 gtacagggtg cactttgtaa ccatttggga caccggacac tcgctgtttt cgaaattacc
    14461 ctttaagcgc gggtattgaa ccaggcttat gcccaagatc gtagcaagca tttaaatgac
    14521 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagtt
    14581 aattaa
//
