LOCUS       sequence-217867-       16980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 16980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU16.1-2.2
FEATURES             Location/Qualifiers
     source          1..16980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1488..1787
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4372..4592
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4593..5396
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(13127..13147)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13127..13145)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(13484..14072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13564..13583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14243..15034,15035..15103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14866..14885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15104..15208)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15235..16577
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(16198..16245)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     16618..16636
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(16955..16971)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16955..16971)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggccacgt actctagccg tcttccttct cctctcaagc
      541 ctttggaaca catctggcgt acggcatacg cttttacgaa gtctgactac aagacagtcg
      601 tcttcccagt catctgctat gggataatgg ctgctccaca gatgtccatc accagacttc
      661 ctcacctcgc ggtatgggtg tggcttcacc ttctgcagtt ctgtgcagcg aatcaggcgt
      721 tgagccctga agaagacgca ttgaataagc cgtatagacc catcccagcg ggcttaattt
      781 cgattcgggg tgccagagtc cttcgttggc tcctcgtccc ggtctgcctg gcgctctcgt
      841 ggacgctgga cgttatctac ccagggatca gtctagccat ctcgttcgtc gtctacaacg
      901 agctcggctt ggataactac tggtatacga agaacttcct caacgctgtg ggcctcgtct
      961 cctggaatat cggcgcggcc aagatcgcat gtgcagagaa tgtttccctc gaccagcggg
     1021 cctggtttgc gccttacgtt agcacgcttc ttatcgctac gacgatacat atccaagact
     1081 tccgcgatga ggcaggagat aggaaacagg gacgagtcac attccccgtc gtgatgcccg
     1141 aattctctag gcgcatgaca ttcctcatcg tcgcagtgtg gtcgttcggc ctcgcgtcgt
     1201 attggaacct aagcagcgtc gcatctccgt ggttcgtcac actggggatg tacgttgccc
     1261 tcagagtgct ccagcagcgc accgagcacg aagataaggt ctcattgcgg atatacatgg
     1321 tacgttgtca tcccccgaac gcacccactt catccccgcc taacccgata acctgttcag
     1381 ttctggctgt gcgttgctca aatcctcccc ttctggacaa ggcaaatgga gggaatagcc
     1441 cagccgtggc tgccaaatgc tcagttcggg gctattcaga acaatgagcg gatctcttat
     1501 gtctttacga tttatagttt tcattatcaa gtatgcctat attagtatat agcatcttta
     1561 gatgacagtg ttcgaagttt cacgaataaa agataatatt ctactttttg ctcccaccgc
     1621 gtttgctagc acgagtgaac accatccctc gcctgtgagt tgtacccatt cctctaaact
     1681 gtagacatgg tagcttcagc agtgttcgtt atgtacggca tcctccaaca aacagtcggt
     1741 tatagtttgt cctgctcctc tgaatcgtct ccctcgatat ttctcatttt ccttcgcatg
     1801 ccagcattga aatgatcgaa gttcaatgat gaaacggtaa ttcttctgtc atttactcat
     1861 ctcatctcat caagttatat aattctatac ggatgtaatt tttcactttt cgtcttgacg
     1921 tccaccctat aatttcaatt attgaaccct cacgatccag ttctccagtg acacagcctt
     1981 tatctggtca aacctttctt tctaatcacc tatgctgatg cttaattaag ggatttttgt
     2041 ctccatcaac ggcatgcgcc caaaaatgac gtttttttta acccatagac acgaaactac
     2101 ccattttcca ccggcctgac ctaccaccgg aacaacggcc atctccaact tgcaagttgg
     2161 ggaaattaag agcatcgcag gtttaatgga agaaaaaaaa aaggtacagc acagcgcaaa
     2221 tggagttagt tcccttatgt cacacactca cacacagtcg gtcagatcaa gcatactggg
     2281 tgcgtataaa tagagtggcc attgccaccc tgtttatctc aaaatctgtc ttgttagtgg
     2341 tcttctccct ttttcaggtt acaattctct tgtttctact tagtatataa gtatatcaag
     2401 ctatattaag catactatca actgtcaact ctatcctcaa aatacaatac aaaatgatgc
     2461 gtacttcgtt tgcaatcctc gcggcgatta tcccgctaac attcacgttt ggtgcttcgg
     2521 tagagaatct tctgcccaga aacaccaccc aaaagcaaga ccttacacca gctcaatgct
     2581 gcgacggcct gaagcgtatc ttagaccctt ctaaagtcct tctgccaggc tcccctgagt
     2641 atgcggctca ggaggcctcc tattactccg gcgaacaggt cctacagttc cctgtctgcc
     2701 gagtcgcacc caccagtccc gaagacgtct caagtataat caaatttgct accacaagta
     2761 actgcaattt tgccgtacgt tcgggcggtc atatgaactg ggttggttca tcgaacatcg
     2821 gtcccgaagg gtttactatc gacttggagg acatgaagca gattgagctt ctggacgatt
     2881 cgaagactgt cgctatctcc ccgggattga cctgggccga tgtctatacc ttcttacgtc
     2941 cccacaatct gcatactgcc gggggccggt cgtcccacgt cggtgtaggt ggcttccttc
     3001 ttggaggtgg catctcattc ctctccctcg agcacggttt cggaagcgac aacatcatca
     3061 actaccaaat cgtcttagcc gatggctcga tcgtcaacgc caatgaagcc gagcgaagcg
     3121 acctgtactg ggctctcaaa ggtggtagca cgaactacgg catagttacc cgctttgaca
     3181 tggcgacgtt cccgttggat caaatgtggg gaggatctct ctactacaac atcagcctgg
     3241 gccctaccct cctcgattat ctcgtcgggt ttacggagag actcgccgtc gatgccaatg
     3301 gcctattcgc gctgtctatc gcttggaacg cagccaataa tgattacctc atttggtcac
     3361 cgaacgccta cctcgcacct acacccttcc caagcccact ctacgatgga ctcgaggaaa
     3421 tcccgacggt gcgcgatacc atgcgcatca caaacctcgt atctgtcaca gacgaaatca
     3481 atatgtcatc cccaggcgga agccgaactc agtggttcgc tttggtgttc aaggcagacg
     3541 cgaccctgcc ttgggatatc tatcagaagg gcgctgatat tatggcgccg ttccttggga
     3601 aggaggggac ctcgtgggcg ctcacgttac aacccattaa tgtcggcatg atcgctgctg
     3661 ctgcgaatcg tggaggaaac ccattcggtc tcaccgaaaa ggatggcgat ctattccttc
     3721 ttttgatatc catcttctgg actgacccag aggacgacgg aaagatcaag gctacgacgc
     3781 aagacttcct gcgatggtcg acggacactg ccaagcagcg caaccttctc aatcgtttca
     3841 tctacttgaa ctacgcgttg gatacgcagc cagtcttagc gagtttcggg gacgagaacg
     3901 tcaggcgtat gaaggccgtg cagaagaagt acgatccaaa gggtctgctc aaagtgtgga
     3961 aaggtggata caagcttgac gaagtataga caaatcgctc ttaaatatat acctaaagaa
     4021 cattaaagct atattataag caaagatacg taaattttgc ttatattatt atacacatat
     4081 catatttcta tatttttaag atttggttat ataatgtacg taatgcaaag gaaataaatt
     4141 ttatacatta ttgaacagcg tccaagtaac tacattatgt gcactaatag tttagcgtcg
     4201 tgaagacttt attgtgtcgc gaaaagtaaa aattttaaaa attagagcac cttgaacttg
     4261 cgaaaaaggt tctcatcaac tgtttaaaag gaggatatca ggtcctattt ctgacaaaca
     4321 atatacaaat ttagtttcaa agatgaatca gtgcgcgaag gacataactc attcaattca
     4381 attcatcatt ttttttttat tctttttttt gatttcggtt tctttgaaat ttttttgatt
     4441 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     4501 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     4561 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     4621 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     4681 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     4741 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     4801 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     4861 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     4921 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     4981 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     5041 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     5101 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     5161 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     5221 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     5281 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     5341 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     5401 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     5461 atcagttatt accctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5521 atcaggatag gaaaaaaccg agcttccttt catccggcgc ggctgtgttc tacatatcac
     5581 tgaagctccg ggtattttaa gttatacaag ggaaagatgc cggctagact agcaagtttt
     5641 aggctgctta acattatgga taggcggata aagggcccaa acaggattgt aaagcttaga
     5701 cgcttctggt tggacaatgg tacgtttgtg tattaagtaa ggcttggctg gggatagcaa
     5761 cattgggcag agtatagaag accacaaaaa aaaggtatat aagggcagag aagtctttgt
     5821 aatgtgtgta acttctcttc catgtgtaat cagtatttct acttacttct taaatataca
     5881 gaagtaagac agataaccaa cagcctttcc cagatataca tatatatctt tatttcagct
     5941 taaacaataa ttatatttgt ttaactcaaa aataaaaaaa aaaaaccaaa ctcacgcaac
     6001 taattattcc ataataaaat aacaacatga gcgcgaatgt atcatggatc cccacagcac
     6061 cggcgacgtt ctggtcttca ccaaacgacc aattctctac atggcctaag ctcgctgtgt
     6121 tggcgttatc caccttcatc attcatcgac tgtacaagtc ggcgtcgttg aacatgaagc
     6181 tgccccccgg gccaaggggg tggcccgtta ttgggagtgt attcgatatt ggaccacata
     6241 tgtggctcac gttcacagag tggaagaagc aatatggacc gattttctat gtgaacctcg
     6301 cagggcgttc gatgatcgtc ttgaacaccc acgaagtcgc cacggagctc cttgataaga
     6361 ggtcgtccat ctactcagac agaccccgcc atatcgtcgc ctctgagatc atgtcaggcg
     6421 aatacctact cggattcatg catttcgacg ataagtggaa gcgtgtcagg agaggttctc
     6481 atgaggcgat gaacaaccaa atcaccaaaa cctaccgtcc gttcatgcaa gtcgaaggat
     6541 acctcctcgc cgacagcctc atgaaagcgc cttctatgtg gaacgatcac atcaagcgtt
     6601 ctgcggcatc tttcatcatg tccgtcgtat acggcaccaa gtccatgcgc acctccaccg
     6661 accccaccct catcaccctc cacaagtttc tgaggagatc gctcggcgcg gccgctggga
     6721 agtactgggt cgagttcttc cactttttgg agtacatgcc aaggtggttg gctccgtgga
     6781 gggtggaggc cgaggagtgg ttcaagacgg acagcaagat gttcgagggg ttctacaacc
     6841 aagttaagaa gaggatggcc gacggagaca acaggccgtg cacagttacg actcttatcg
     6901 aagaacaaaa aaagaaaaac ctctctacta tggaatcagt ctggctggca gcggctatgt
     6961 acggtggagg ctccgaatca actgctggat ccctcggctg gtggataact gccatggtag
     7021 tctacccctc cgccctccgc gccgcccaag aagagctcga tcgcgtcgta ggccgagatc
     7081 gtatgcccac cttcgacgac tatgaccacc tcccctactg cagggccatg gtcaaggagg
     7141 tcttgaggtg gcgacccgca acccccctcg gtgtccccca tcgcgtggaa agggatgact
     7201 ggttcaacgg ttacttcatc ccgaagggaa cattggttgt ggcgaacatt tggggcatga
     7261 accacgacga gaaagtctac gggcccgacg ttcacgagtt caacccggcc cgtttccttg
     7321 aaggatctgg aaagcccctg ccctctgtct cagataccaa ggacgagggc catgttacct
     7381 acggcttcgg ccggcgcatc tgcgtcgggc gccacgttgc caacaacaac ttgttcatcc
     7441 agttcgcgag catggcttgg gcatgcaata tccaggcgaa gaaggatgcg caggggaacc
     7501 cgatcatgcc tgatgcggat aagtgcacgt cggatttgac gatgcacacc gtcccattcg
     7561 attgcgatat aacaccaagg ttccccgaag ttccgtccat cattgcccag tccattgagt
     7621 tgaataatta cggccaaatt tgaagtgctt ttaactaaga attattagtc ttttctgctt
     7681 attttttcat catagtttag aacactttat attaacgaat agtttatgaa tctatttagg
     7741 tttaaaaatt gatacagttt tataagttac tttttcaaag actcgtgctg tctattgcat
     7801 aatgcactgg aaggggaaaa aaaaggtgca cacgcgtggc tttttcttga atttgcagtt
     7861 tgaaaaataa ctacatggat gataagaaaa catggagtac agtcactttg agaaccttca
     7921 atcagctggt aacgtcttcg ttaattggat actcaaaaaa gatggatagc atgaatcaca
     7981 agatggaagg aaatgcgggc cacgaccaca gtgatatgca tatgggagat ggagatgata
     8041 cctccattgg gccgatgaag ttagtcgacg gatagaagcg gttgtcccct ttcccggcga
     8101 gccggcagtc gggccgaggt tcggataaat tttgtattgt gttttgattc tgtcatgagt
     8161 attacttatg ttctctttag gtaaccccag gttaatcaat cacagtttca taccggctag
     8221 tattcaaatt atgacttttc ttctgcagtg tcagccttac gacgattatc tatgagcttt
     8281 gaatatagtt tgccgtgatt cgtatcttta attggataat aaaatgcgaa ggatcgatga
     8341 cccttattat tatttttcta cactggctac cgatttaact catcttcttg aaagtatata
     8401 agtaacagta aaatataccg tacttctgct aatgttattt gtcccttatt tttcttttct
     8461 tgtcttatgc tatagtacct aagaataacg actattgttt tgaactaaac aaagtagtaa
     8521 aagcacataa aagaattaag aaaatgagcc acttctcatg gtccaacatc tccagcgcag
     8581 cgccctcttc ctcttcatac gctgagctct acccctctct cacgtcatgg acactcagtg
     8641 ttccggtcgc catagtgctc cttgtagcag cgcgttccta tatggcaagg cctaagggga
     8701 agcttcctcc tgggccgcca ggattacccg tcataggaaa tctgcatcaa ctgacagata
     8761 agctctgggt aacgctcgag aagtggacta aacaatacgg ccccgtagtc tatctaaata
     8821 tcgccggcca aaacacaatc gtccttggca cgcacaaagc cgccgccgat ctcctcgatc
     8881 gtagagcggg catctactct gaccgaccac gaaatatcgt cgcaggagag ctgctgacgg
     8941 gcggtttggt atttgcgttc gctcagcata acgatgtttg gaagcggatg agaagggcat
     9001 cgcatgaagc gatgaataac caagtcgcca agtcctactt cccattccaa gagactgaat
     9061 cgtctctcct tgtctctgag ctcctcgctt ccccggacct ctttgactcc cacctccgcc
     9121 gcgcgtccac gtctctcgtc ttatccatga tctacgggct gcctcccatg gaagacagca
     9181 gggaccccgt catcttgcgg gtgaatcatt tcacggagag ggcgctgaag gcggcgatcc
     9241 cgggcgcgta tttggtggag tacttcacgt ggatggagca tttgccgcgg tggatgtgtg
     9301 cctggagacg atatgctgag gatgcattca aacgcgattc cgtcctcttc gaaaacctct
     9361 tcgctgatgt tgtgacccgc ctcaaaagcg gtgatcaacg ccccagcgtc gctgccacca
     9421 tcatcaccga acaagagaag aagaacctca cggataaaga agctgcctgg gtatctgcta
     9481 cgctctatgc cgctggtgct gagacgacat cgggccaact cgcctggttt atggaggcaa
     9541 tggttctcta ccccgaggtg caagctcgcg cgcaagcaga gattgatcgc gtcgtcgggc
     9601 gcgataggat gcctacattc caggactacg accatcttcc atacatcaag gcaatggtga
     9661 aggagacgct gaggtggaac ggcgtcggca gattgggtgt cccacacagg ctggcccagg
     9721 acgattggta cgaggggcac ttcatcccga aggacacgat ctgcttagtg aatgtttggg
     9781 gattgaacca cgatacggag gtctacggtc ctgacgtcga tgatttccgc ccggagaggt
     9841 tcttgaacga agaaggggag ctgaaggcca tgccaacaga taccaaggac gaagggcacg
     9901 taacctatgg cttcggaaga cggatttgtg ttggccgcca tgtctccaac aacagcatgt
     9961 tcatcgagat cgcctctatc ctgtgggcgt gcaatattcg ccctgggaag aacgaggctg
    10021 gagagatcgt tatgccggac ccgcagaata gtatcgatga ggggcttgta gtccgtccag
    10081 cacccttcct gtgctccatt accgctaggt cgcctgaagt cgagactatc ctcgctgaga
    10141 cgaaggatcg tcatggttta cctatttaga tttaactcct taagttactt taatgattta
    10201 gtttttatta ttaataattc atgctcatga catctcatat acacgtttat aaaacttaaa
    10261 tagattgaaa atgtattaaa gattcctcag ggattcgatt tttttggaag tttttgtttt
    10321 tttttccttg agatgctgta gtatttggga acaattatac aatcgaaaga tatatgctta
    10381 cattcgaccg ttttagccgt gatcattatc ctatagtaac ataacctgaa gcataactga
    10441 cactactatc atcaatactt gtcacatgag aactctgtga ataattaggc cactgaaatt
    10501 tgatgcctga aggaccggca tcacggattt tcgataaagc acttagtatc acactaattg
    10561 gcttttcgct agtcttatct aaaaattgcc tttatagtcc gtctctccag tcacggcctg
    10621 tgtaactgat taatcctgcc tttctaatca ccattctact gtttaattaa gggattttgt
    10681 cttcatcaac ggcttccgcc caaaaaaaag tatgacgttt tgcccgcagg cgtgaagctg
    10741 cccatcttca cgggcctgac ctcctctgcc ggaacaccgg ccatctccaa ctcataaatt
    10801 ggagaaataa gagaatttca gattttcaga ggatgaaaaa aaaaaggtag agagcataaa
    10861 aatggggttc actttttggc aaagttacag tatgcttatt acatataaat agagtgccga
    10921 taatggcttt ttttcatctt cgaaatacgc ttgctactgc tcttccagcg tttttattac
    10981 ttctttcttg tttctcctta gtatataaaa tatcaagcta caacaagcat acaatcaact
    11041 gtcaactgtc aattatatta taatacacta tgcgcgccga ggcttacctt gctctattcg
    11101 cactggcgac aaggtcgttc gccaccaccc agcagccact tcagacacaa acgtttgacg
    11161 aaggagtgcg gactacagca ctacaatgtt gcaacgcact cgaagacatc ctaacttcct
    11221 ctaccatcct atatcccgcc tcatctaaat attccgcgca gtcatctaca tattactccc
    11281 tcgaacaagc cgcccaaaag ccagcgtgtc gcatagcccc cgccacaacc gaagacctct
    11341 cccgattaat aaaattcgcg actgccacaa ggtgcgagtt cgccgtccgc gctggcggac
    11401 acatgagctg ggaaggctcg tcgaatatcg gtccaaacgg atttaccatc gaccttgggg
    11461 gcttgaagga ggtggaactg aaggatgatg ggaagacggt ttctatcgct cctggtctga
    11521 aatggtcaga agtttatgga ttcctgaagc cacacgggct tcacacggtg ggtggacgct
    11581 cctcaggcgt tggcgtcggt ggtttcctta taggaggtgg tgtctccttc ctgtcaacgg
    11641 aaatgggtct cggaagtgac aacgttctcg attacgaagt agtccttgct gatggatcca
    11701 tcgtccacgc aaacgagtca gaacgctccg atctttactg ggcgttgaaa agcggcagca
    11761 ccaactacgg gatcgtcacc cgtttcttga tgcccacaat accccgccct cagatatggg
    11821 gtggctcgct cttctatcac gggagtgcgg gtcccaggct attcaagcaa cttgctacgt
    11881 tcaccgatcg tctcaaggag gatcctcacg gcatgtccgc catctccgtt gcgtggagtc
    11941 cagaaatgaa agactacgct atctggtccc ccaacgtcta cctcgcgcca caagcatacc
    12001 cctcccccct tttcgatggc ctccaagata tcgagccatt ccaaagcacg atgcgcgttg
    12061 cggatctagg cgagatcacg gacgaagtag acatgctctc gccgggtggg cggaattcgc
    12121 agtggttttc tgtcgttttc aagaatgatg cagagctgcc ttgggatatt cagctgaagg
    12181 gcagggaaat ttttgagccg taccacgatc gtcccggtgc cgcatgggcg atcaccgttc
    12241 agcccatcaa cgaaggcatg attgccgctg gacacaagcg agggggaaac ccgttaggcc
    12301 tctcagtcga ggatggcgat caactccttc tacttggctg cgtcttctgg aacgatcctc
    12361 aagatagtgc cgtcatgaag gccaaaaccc aagagtacat caaagcagcg acggagttgg
    12421 ctcgcgaacg tggactgctg aacaagtaca tctacttgaa ctatgcattg gatacgcaaa
    12481 acgtcctgga gagctacggc gcagggaacc tgaagaagat gagggaaatc aagagtaaat
    12541 acgaccccga caaccttctg gaagtatgga aggggggctg gaaattgtaa ggagattgat
    12601 aagacttttc tagttgcata tcttttatat ttaaatctta tctattagtt aattttttgt
    12661 aatttatcct tatatatagt ctggttattc taaaatatca tttcagtatc taaaaattcc
    12721 cctctttttt cagttatatc ttaacaggcg acagtccaaa tgttgattta tcccagtccg
    12781 attcatcagg gttgtgaagc attttgtcaa tggtcgaaat cacatcagta atagtgcctc
    12841 ttacttgcct catagaattt ctttctctta acgtcaccgt ttggtctttt atagtttcga
    12901 aatctatggt gataccaaat ggtgttccca attcatcgtt acgggcgtat tttttaccaa
    12961 ttgaagtatt ggaatcgtca attttaaagt atatctctct tttacgtaaa gcctgcgaga
    13021 tcctcttaag tatagcgggg aagccatcgt tattcgatat tgtcgtaaca aatactttga
    13081 tcggcgctat gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
    13141 taattgcgcg cttggcgaga ctcaaacaag atatattttg cccgccttac agacgaaact
    13201 agttggaggt tatggagcat actatcacgt gggcggccac tggtgagtta ctacacccca
    13261 ggggcaacgt tgatgctcct aaaaaactct ggctggacgc aagccgtaac acccgtgtca
    13321 cttcataatc gtttgcaatt cagggcttga tctacacatt accctgttat ccctattaat
    13381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    13441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    13501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    13561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    13621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    13681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    13741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    13801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    13861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    13921 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    13981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    14041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    14101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    14161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    14221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    14281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    14341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    14401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    14461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    14521 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    14581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    14641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    14701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    14761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    14821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    14881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    14941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    15001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    15061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    15121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    15181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa
    15241 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
    15301 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
    15361 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
    15421 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
    15481 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
    15541 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
    15601 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
    15661 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
    15721 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
    15781 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
    15841 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
    15901 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
    15961 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
    16021 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
    16081 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
    16141 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
    16201 gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa
    16261 acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca
    16321 cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt
    16381 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc
    16441 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt
    16501 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt
    16561 tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac ctataaaaat
    16621 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctcggc
    16681 gcgccattac cctgttatcc ctaatataca acgcaaagaa gctggaaaat tggcgaggga
    16741 atcctgtttc tgtctatcca agaatgggca tgaggtggca accgtcgtgc tagcgtacag
    16801 ggtgcacttt gtaaccattt gggacaccgg acactcgctg ttttcgaaat taccctttaa
    16861 gcgcgggtat tgaaccaggc ttatgcccaa gatcgtagca agcatttaaa tgacggtatc
    16921 gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag agttaattaa
//
