LOCUS       sequence-217868-       11013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU17.1-2.2
FEATURES             Location/Qualifiers
     source          1..11013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1347..1646
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4177..4397
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4398..5201
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(7160..7180)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7160..7178)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7517..8105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7597..7616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8276..9067,9068..9136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8899..8918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9137..9241)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9268..10610
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10231..10278)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10651..10669
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10988..11004)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10988..11004)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgttcagcc ttctccgacg acacctttac accctcttcc
      541 tcttcacgta cagcgacatg aagaccatct tgcttccaat aactgtcttt gcctcggtag
      601 cagcccctgc gcagtcgcca caaggcctcc tactcggagt cttctggaca tggttgcacc
      661 agctccacgc caacctctca aaccagtaca aggcgctgga ggaagacaag atgaacaagc
      721 cctggcgacc cttgcccgcg caccgcgtca ctccccggca ggccgagctc ctgcgctgga
      781 ccctgatccc actgacgatc ggcttctcca ctatcggcgg cccacacgcc atcgccgcca
      841 gtatctggct cgcaacagcc ttggtcatct acgagcagcc ctccgtcagc ggccactggc
      901 tcggcaagaa catcctcaac tgcgccggct acatcggttt cgaatacggc gcaacagcaa
      961 tgctgggcga gggcaggcct ctgagcgcgt cggcggtgct cttgctgcta tgcagcggcc
     1021 tcgtcatcct caccaccatc cacgcgcagg acttcgccga catcgccggc gactccgccc
     1081 tcggccgcgt caccttcccc atccgcgccc ccgccctctc ccgcgcgttc atctacctcg
     1141 gcatcccgct cctctccctc gcgctctccg tcgcctggga tatgttctgg gcggcccgcg
     1201 tactgttcct cgcgctcggc tccgcagtcg cgtggcggtt ctacgccttc cggacgatct
     1261 cgcaggacgc gcggtcgtat gtgttgtaca atgtatggct attcagtgta caccttttgc
     1321 ctgccacgca gcggcactgg atgtgagcgg atctcttatg tctttacgat ttatagtttt
     1381 cattatcaag tatgcctata ttagtatata gcatctttag atgacagtgt tcgaagtttc
     1441 acgaataaaa gataatattc tactttttgc tcccaccgcg tttgctagca cgagtgaaca
     1501 ccatccctcg cctgtgagtt gtacccattc ctctaaactg tagacatggt agcttcagca
     1561 gtgttcgtta tgtacggcat cctccaacaa acagtcggtt atagtttgtc ctgctcctct
     1621 gaatcgtctc cctcgatatt tctcattttc cttcgcatgc cagcattgaa atgatcgaag
     1681 ttcaatgatg aaacggtaat tcttctgtca tttactcatc tcatctcatc aagttatata
     1741 attctatacg gatgtaattt ttcacttttc gtcttgacgt ccaccctata atttcaatta
     1801 ttgaaccctc acgatccagt tctccagtga cacagccttt atctggtcaa acctttcttt
     1861 ctaatcacct atgctgatgc ttaattaagg gatttttgtc tccatcaacg gcatgcgccc
     1921 aaaaatgacg ttttttttaa cccatagaca cgaaactacc cattttccac cggcctgacc
     1981 taccaccgga acaacggcca tctccaactt gcaagttggg gaaattaaga gcatcgcagg
     2041 tttaatggaa gaaaaaaaaa aggtacagca cagcgcaaat ggagttagtt cccttatgtc
     2101 acacactcac acacagtcgg tcagatcaag catactgggt gcgtataaat agagtggcca
     2161 ttgccaccct gtttatctca aaatctgtct tgttagtggt cttctccctt tttcaggtta
     2221 caattctctt gtttctactt agtatataag tatatcaagc tatattaagc atactatcaa
     2281 ctgtcaactc tatcctcaaa atacaataca aaatgtcgta tgctcttttg gcagcaggcg
     2341 cggcttgcat cttgctcgtc gggaatggcc tctacaccct gctgtggcat cccctcgcct
     2401 cataccctgg cccacgactt gcggcgttga cgaagtggta ccgcgcgtac ttcgacctcg
     2461 tccacgatgg cggttggctt gagcacctgg aggagcttca tcgtcagtat ggccctgtcg
     2521 tacgcgtcgc tgcttctgaa ctacacttcg cggagcccgc agcataccat gacatccaca
     2581 acttcacaaa ccgttttgca aaggaccccg agttgtatcg tgccttcgac ctgccacagg
     2641 cgtccttcgg acaggtggat cctgtcgcgg cgaagacccg ccgcgacacc ttgggaccac
     2701 tcttctcacg tcaaggtatt cgcaagttcg agttcgtcat tcaggataag gtcgacctcc
     2761 tcgtcaaccg cctcatcgag gagggctcca cccgccagcc cgtcaacctt ttcctcgcct
     2821 tccgctcctt cagcttcgac accatcatgt cctactgctt cgccacctcc taccactccc
     2881 tcgagcacga acgcttcgcc caccccatga tccccatcat ctttaaggtc aacgtcctca
     2941 cctggaccct gcgttacttc cccttcctca tgagccttgt gcgctacggt ccgcgcttca
     3001 tcacctcccg gctctcgccc gtagccgttg gcttccaggg cctgttcgac cactttgaca
     3061 agcgggtgga tgaggtgttg agcgagccgg agagcctagg ggccgccggg catgatacgg
     3121 tattccatca tctgatcaat gggaagagag agaggccgag caaggaggtg ctgatcgagg
     3181 aggcaatcac actgtttggg gcggggagcg agacgacggc ggcggcggtg acaacggcga
     3241 ttttccatgt tctgaacgac aagcgcatcc gcgagagggt gatggaggag ctaagggagg
     3301 catggccaga tccgacgatg cggttgtcgg cgcaggagat cgagaagctg ccgtatttga
     3361 cagctgtcat caaggaaacg ttgaggttcg cgccaggtgt cgtcactcct gcccctcgca
     3421 tcgtgcatca ggactccgtc atcgcgggcc acccaattcc tgctggaacg accgtggcca
     3481 taggtgccac attcgtgcac cttaacccgg aaatctacga ggacccaaag aggttcaagc
     3541 ccgagcgatg gctgaaggag ggcgcgcagg cactcgagag tgcatacttt atcccctttt
     3601 cgaagggccc gaggatgtgt ctggggcatt accttgcgtg gtgcgagatg tacctcgtcc
     3661 ttgcgaacgt cctacgcaag acggatatca ctgtgttcga gacggatgag acggactttg
     3721 agttccgtca atactggatg cccatcttcc gaggacgaca gctgaagggg ttcgtcgagc
     3781 cgagaattga ctaaacaaat cgctcttaaa tatataccta aagaacatta aagctatatt
     3841 ataagcaaag atacgtaaat tttgcttata ttattataca catatcatat ttctatattt
     3901 ttaagatttg gttatataat gtacgtaatg caaaggaaat aaattttata cattattgaa
     3961 cagcgtccaa gtaactacat tatgtgcact aatagtttag cgtcgtgaag actttattgt
     4021 gtcgcgaaaa gtaaaaattt taaaaattag agcaccttga acttgcgaaa aaggttctca
     4081 tcaactgttt aaaaggagga tatcaggtcc tatttctgac aaacaatata caaatttagt
     4141 ttcaaagatg aatcagtgcg cgaaggacat aactcattca attcaattca tcattttttt
     4201 tttattcttt tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac
     4261 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
     4321 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca
     4381 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
     4441 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
     4501 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
     4561 tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta
     4621 agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg
     4681 ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat
     4741 gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc
     4801 aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat
     4861 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
     4921 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
     4981 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc
     5041 aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa
     5101 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
     5161 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
     5221 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattaccct
     5281 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gataggaaaa
     5341 aaccgagctt cctttcatcc ggcgcggctg tgttctacat atcactgaag ctccgggtat
     5401 tttaagttat acaagggaaa gatgccggct agactagcaa gttttaggct gcttaacatt
     5461 atggataggc ggataaaggg cccaaacagg attgtaaagc ttagacgctt ctggttggac
     5521 aatggtacgt ttgtgtatta agtaaggctt ggctggggat agcaacattg ggcagagtat
     5581 agaagaccac aaaaaaaagg tatataaggg cagagaagtc tttgtaatgt gtgtaacttc
     5641 tcttccatgt gtaatcagta tttctactta cttcttaaat atacagaagt aagacagata
     5701 accaacagcc tttcccagat atacatatat atctttattt cagcttaaac aataattata
     5761 tttgtttaac tcaaaaataa aaaaaaaaaa ccaaactcac gcaactaatt attccataat
     5821 aaaataacaa catggcctcc atcctcaagt cgaagtgcgt cctcgtcacc ggcgcgaccg
     5881 cgggcatcgg gcgcgcgctc gctcgcgcca tccgtgacct cccctcgaag ccgactgtca
     5941 tcgcgatggg ccgccggcag gagcggctcg atgagcttgc gaaagagggc ttcaagacgg
     6001 ccaagttcga catgaacacg acgcccgaca agatcagggc gttcgtggat gatatcacgg
     6061 cgcagtaccc ggagctggac gcggtcatcc tgaacgcggg cgtgcagtat atcttcgact
     6121 tcaccaagcc cgagagcgtg gatttcaaca agatcagcac cgagttcaac gtcaactacc
     6181 tcgccgtcgt gagcatgatg acggccttcc tcccgcacct aatcaagatc gggaaagagc
     6241 gccccaccct cctcgtcccg gtgacctctg gcctcatgac gatcccccta ccgagcacgg
     6301 cgaactactc tgcgacgaag gcggccctcc acagcatctc cttctccatc cgcattcaaa
     6361 tggagaacgc caagacaaac gtgcacgtcc tcgagattat ccctcccctc gtcgagtccg
     6421 agttgcacga caaccaaggc accacggagg ctctgtccag attctggatg ccccttgccg
     6481 actttatcaa cgtcgtcctc gacggcctca aggagggcaa gatcgccatc accgccggcg
     6541 cggccacctc agcgtacctc cagttcgaga agggcaagga gagctaccgc aatgggctca
     6601 tggggctgcc agttacgcag taaggagatt gataagactt ttctagttgc atatctttta
     6661 tatttaaatc ttatctatta gttaattttt tgtaatttat ccttatatat agtctggtta
     6721 ttctaaaata tcatttcagt atctaaaaat tcccctcttt tttcagttat atcttaacag
     6781 gcgacagtcc aaatgttgat ttatcccagt ccgattcatc agggttgtga agcattttgt
     6841 caatggtcga aatcacatca gtaatagtgc ctcttacttg cctcatagaa tttctttctc
     6901 ttaacgtcac cgtttggtct tttatagttt cgaaatctat ggtgatacca aatggtgttc
     6961 ccaattcatc gttacgggcg tattttttac caattgaagt attggaatcg tcaattttaa
     7021 agtatatctc tcttttacgt aaagcctgcg agatcctctt aagtatagcg gggaagccat
     7081 cgttattcga tattgtcgta acaaatactt tgatcggcgc tatgcggccg ccaccgcggt
     7141 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg agactcaaac
     7201 aagatatatt ttgcccgcct tacagacgaa actagttgga ggttatggag catactatca
     7261 cgtgggcggc cactggtgag ttactacacc ccaggggcaa cgttgatgct cctaaaaaac
     7321 tctggctgga cgcaagccgt aacacccgtg tcacttcata atcgtttgca attcagggct
     7381 tgatctacac attaccctgt tatccctatt aattaatacg gttatccaca gaatcagggg
     7441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7921 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8221 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8581 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8881 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9241 gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa
     9301 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     9361 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     9421 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     9481 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     9541 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     9601 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     9661 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     9721 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     9781 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     9841 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     9901 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     9961 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
    10021 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
    10081 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
    10141 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
    10201 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa
    10261 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
    10321 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
    10381 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
    10441 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
    10501 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
    10561 attggattag tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa
    10621 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    10681 gcgcgtttcg gtgatgacgg tgaaaacctc ggcgcgccat taccctgtta tccctaatat
    10741 acaacgcaaa gaagctggaa aattggcgag ggaatcctgt ttctgtctat ccaagaatgg
    10801 gcatgaggtg gcaaccgtcg tgctagcgta cagggtgcac tttgtaacca tttgggacac
    10861 cggacactcg ctgttttcga aattaccctt taagcgcggg tattgaacca ggcttatgcc
    10921 caagatcgta gcaagcattt aaatgacggt atcgataagc ttgatatcga attcctgcag
    10981 cccgggggat ccactagttc tagagttaat taa
//
