LOCUS       sequence-217869-       18221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18221)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 18221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU20.1-2.2
FEATURES             Location/Qualifiers
     source          1..18221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1392..1691
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4318..4538
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4539..5342
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      11859..12046
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(11914..11938)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(14368..14388)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14368..14386)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(14725..15313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14805..14824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15484..16275,16276..16344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16107..16126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16345..16449)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      16476..17818
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(17439..17486)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     17859..17877
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(18196..18212)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18196..18212)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgcttcttc ttctactatt ctcttttggg tgctccatct
      541 tggaacctac cttgtatcat gtatacactt tatatctatt caccttgact gactacaaga
      601 ccatttttat tccgattacg gttttcgcct gctctacagc tcccatacat tcgatctcca
      661 atcttttcca tggaagcaca tggatatgga tgcacctact gatgtgtaat gtctcgaatc
      721 aactgagagg ggagcaggaa gacgccgtga atcgaccatg gagaccactg ccgtcgaccc
      781 ggatgaccaa gaggcaagcc acgacgctcc ggtgggctat ggtggtgtta tgcctgctct
      841 actcttccgc ctatggtcac cgattagcat acgttactgt cggccttttc atcacaacat
      901 acgtatacga cgatctaggg ttctctcaga gtccgctcgg aaaaagcttt tgtaacgttg
      961 gtggttatgc gactttcgag atcggcgcca caaagatcat gggtgcagaa cacgagctag
     1021 acactacctc acttgcgtcc gtctttctta gcggcatggt gatatttact actatccaag
     1081 tacaggacta tcccgacatc gaaggcgaca cagcgtctgg tcgggtcacc tttccaattt
     1141 atgcacccga gttctcgcga ttattcactc tgtcatcgat cattatctgg tccttcgcct
     1201 tgtcatggtt ctgggatatc ggcatcctga gtcagtgctt tctcgcagga ttgggagtat
     1261 acgtcggtgc tcgttactat ctccatcgga caccaatcgc ggataaaaaa tcatacaagc
     1321 tcttcaacgt ttggctggtg ctgatacatc ttcttccact ccacgcccgc atccgtgtgt
     1381 ttctctatta ggcggatctc ttatgtcttt acgatttata gttttcatta tcaagtatgc
     1441 ctatattagt atatagcatc tttagatgac agtgttcgaa gtttcacgaa taaaagataa
     1501 tattctactt tttgctccca ccgcgtttgc tagcacgagt gaacaccatc cctcgcctgt
     1561 gagttgtacc cattcctcta aactgtagac atggtagctt cagcagtgtt cgttatgtac
     1621 ggcatcctcc aacaaacagt cggttatagt ttgtcctgct cctctgaatc gtctccctcg
     1681 atatttctca ttttccttcg catgccagca ttgaaatgat cgaagttcaa tgatgaaacg
     1741 gtaattcttc tgtcatttac tcatctcatc tcatcaagtt atataattct atacggatgt
     1801 aatttttcac ttttcgtctt gacgtccacc ctataatttc aattattgaa ccctcacgat
     1861 ccagttctcc agtgacacag cctttatctg gtcaaacctt tctttctaat cacctatgct
     1921 gatgcttaat taagggattt ttgtctccat caacggcatg cgcccaaaaa tgacgttttt
     1981 tttaacccat agacacgaaa ctacccattt tccaccggcc tgacctacca ccggaacaac
     2041 ggccatctcc aacttgcaag ttggggaaat taagagcatc gcaggtttaa tggaagaaaa
     2101 aaaaaaggta cagcacagcg caaatggagt tagttccctt atgtcacaca ctcacacaca
     2161 gtcggtcaga tcaagcatac tgggtgcgta taaatagagt ggccattgcc accctgttta
     2221 tctcaaaatc tgtcttgtta gtggtcttct ccctttttca ggttacaatt ctcttgtttc
     2281 tacttagtat ataagtatat caagctatat taagcatact atcaactgtc aactctatcc
     2341 tcaaaataca atacaaaatg aagcgcttgc cttgcattgc cctttcaccc tacgcccatc
     2401 ttcctacgct gcgcatcatg aactatatgc tatcctttct cgttctactt gtatcacttt
     2461 tgatgctcag aatcatactg aagaggaaca cagacaactc tggtgcccat cattatcctc
     2521 ctggtccgcc cgcaataccc gtccttggta atatcatgac tattccgaac acgggaccat
     2581 ggaaattatt ctcaaagctt catcgtgact acggagaacc tatcttcttt tggggcttag
     2641 ggaacagcat ccttgttctc agcacaatga cttccataac agatctcctc gacaagcgag
     2701 gggagaacta ttctcatcgg ccggtattga ctgttttagg agaactgatg ggattggatc
     2761 gtagcatgcc gatgttaccg tacgggcgtg agtggcgaga gcagagaaag ctcgcccacg
     2821 ttgccttgaa cccatctgcc gtgagaaagt atcacatcgt gcaggagaac ctagcttctg
     2881 tattggcgct tggtattctc acaaaccccg aaggattctt tgataatgta cgactttacg
     2941 cggagcgcat aatattctct gtggtatacg gtgtgtacat cgatggttcc aataatgagt
     3001 atgtcactca ggcagaagaa accatgaaaa ttgtgggaga cgcaatggta ccaggagcgt
     3061 tcatctgtga cttgttgcct tggatgaagc atcttccttc gtggttgcca tttcgaaagc
     3121 aagcacatta tgggagaagt atgattgaag cccttgtgac caaacccttc gaacacgttc
     3181 aacgagagat gatgacgaat atcgctgcac catctctcac tcgagaacta ttgactttgc
     3241 aacgtaacga tttcgcgtct tttgagagct ccgttaagtg ggcgactgga tctttatatg
     3301 gtggtaggca taacatttgg gtcttttcaa agaatttttg tctcgcactg acgggaactg
     3361 gactgtcagc gggcggcgaa acgacttacg ccaccatcct tactttcatg atggcaatgg
     3421 caatgaatcc cgaaactcaa cgtaaagccc aaaaggaggt cgatgaggtt atagggatac
     3481 atagacttcc cgtcgtctcg gacaagacac agctgaaata cgtagcagct gtcatcaacg
     3541 agacaatgag atggcatcct gtgttaccgc tgggcatcgc tcgccgtacg gaccatgacg
     3601 atgtgtacat gggatacttc ataccaaaga atactgttgt ttttcctaat gcatgggcgc
     3661 tcacaaggag gcacagcgac acgtacgatc cagaacaatt catcccggaa cgttttatcg
     3721 acccttccga agcaacggac gatccttcca cttatttgtt tggatttgga agaagagtct
     3781 gtccgggtaa atctctggcg gagaactcgg tattcattgc aatcgcgact atcctatctg
     3841 ccctagatat ctcagaagac ccggacagta gaataacccc agcatttacg gaagctttag
     3901 taaggtatga ttctccccgg gagctctaca aatgaacaaa tcgctcttaa atatatacct
     3961 aaagaacatt aaagctatat tataagcaaa gatacgtaaa ttttgcttat attattatac
     4021 acatatcata tttctatatt tttaagattt ggttatataa tgtacgtaat gcaaaggaaa
     4081 taaattttat acattattga acagcgtcca agtaactaca ttatgtgcac taatagttta
     4141 gcgtcgtgaa gactttattg tgtcgcgaaa agtaaaaatt ttaaaaatta gagcaccttg
     4201 aacttgcgaa aaaggttctc atcaactgtt taaaaggagg atatcaggtc ctatttctga
     4261 caaacaatat acaaatttag tttcaaagat gaatcagtgc gcgaaggaca taactcattc
     4321 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
     4381 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     4441 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     4501 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     4561 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     4621 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     4681 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     4741 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     4801 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     4861 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     4921 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     4981 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     5041 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     5101 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     5161 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     5221 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     5281 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     5341 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     5401 aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5461 taccgcatca ggataggaaa aaaccgagct tcctttcatc cggcgcggct gtgttctaca
     5521 tatcactgaa gctccgggta ttttaagtta tacaagggaa agatgccggc tagactagca
     5581 agttttaggc tgcttaacat tatggatagg cggataaagg gcccaaacag gattgtaaag
     5641 cttagacgct tctggttgga caatggtacg tttgtgtatt aagtaaggct tggctgggga
     5701 tagcaacatt gggcagagta tagaagacca caaaaaaaag gtatataagg gcagagaagt
     5761 ctttgtaatg tgtgtaactt ctcttccatg tgtaatcagt atttctactt acttcttaaa
     5821 tatacagaag taagacagat aaccaacagc ctttcccaga tatacatata tatctttatt
     5881 tcagcttaaa caataattat atttgtttaa ctcaaaaata aaaaaaaaaa accaaactca
     5941 cgcaactaat tattccataa taaaataaca acatggcgac tgtcatgtat tctcttctcg
     6001 ttacgaactg caccgtagtt cacgttgctg tgctcatttc aatcgtgctt gtataccgca
     6061 ggctttttgt aggacggaga tcttctcttg atccgttgcc ctatccagat gaagaaccgg
     6121 agtggatatg gggtcacgaa aaacgcgttt atgacagccc tccagggcag gcgtacttac
     6181 ggtggatgaa gagtgttggg catacctacc gcattcgtgc tgcccttggt gcccctgacg
     6241 ttcttgttct gggcgatccg ctcgccattt cttatattct ccaagagaaa atttacgact
     6301 accaccatag cgctgtggta cgccctaggg tggctcgctt cctgggaaaa ggcttaggtt
     6361 gggtcgaggg agaatcagag cataaacgaa tgagacgtct tgtcagccct gcattctcgg
     6421 cgcaaaccgt taaagacatg gaaggcgaaa tatttcaagc gacgtctacg gctagtctca
     6481 atatcgagga ctcggacggt gaaattggcg taaacatatt gtcatggaca aatcgaatga
     6541 ccctgaattc cattggacgc gtcgcattct tgtacgattt cgaagccggg aagagccgcg
     6601 aggcacagct tatattggac acaaggcgga agtcgctaac cggtattgct cgatacgcct
     6661 cgttcttaac tctgatgatg ctcaggcggt tccctgctct caatagcctt cctgtacccg
     6721 cccttcaggc gcaaagccta gtcacgacaa ccataaagtc tgtcgtggcc aaggagatca
     6781 tagcgagagg ttcttcaaca tctgatctcc gaactcgagg caaggacctg ctaagcagac
     6841 tcttggtagc ccatgatgag cagcaccttt cagtcgagga actacatgag cacatatcga
     6901 catttttagt ttctggacag gaaactacga gccaaacggt cgcgttctcc atcttcgagc
     6961 tttcccgtca cccagagatc caacagcgtc tcagagaaga gttggacaca tcttacccat
     7021 cttctacagg cacgccgcag tcacagctac cttatctgga tgccgtgctt aaggaaacac
     7081 taaggatgta tcctgccctt ccgtacatgg aacgtgtcgc aactaaatct gatagaattc
     7141 cgcttcaaac gcccatcagt cacggtggag atcaggcgct acgggacatt cgtgttgaaa
     7201 agggtcagac cgtcatcatc ccaatcatat ctcttcaccg ctctgatgct atctggggcg
     7261 atggaagtgt ctttcgtccc gaacgatggc ttgaagcttt acctcatgag gcacagcgtc
     7321 ttccagggtg gtccaactta cttgcattca gtgatggacc cagagcatgc cctggaatgc
     7381 gaatggcgat atttgggtca aagcttatat taagctctct tgtcaagaaa tatcgcatag
     7441 aaagtaataa ctcagaagta gttctcagga tttcgtcaag tttgcagcct tgggtgattg
     7501 atgattcgga ccaaggtccg caagtgcctg taacgctgca catgcggtaa agtgctttta
     7561 actaagaatt attagtcttt tctgcttatt ttttcatcat agtttagaac actttatatt
     7621 aacgaatagt ttatgaatct atttaggttt aaaaattgat acagttttat aagttacttt
     7681 ttcaaagact cgtgctgtct attgcataat gcactggaag gggaaaaaaa aggtgcacac
     7741 gcgtggcttt ttcttgaatt tgcagtttga aaaataacta catggatgat aagaaaacat
     7801 ggagtacagt cactttgaga accttcaatc agctggtaac gtcttcgtta attggatact
     7861 caaaaaagat ggatagcatg aatcacaaga tggaaggaaa tgcgggccac gaccacagtg
     7921 atatgcatat gggagatgga gatgatacct ccattgggcc gatgaagtta gtcgacggat
     7981 agaagcggtt gtcccctttc ccggcgagcc ggcagtcggg ccgaggttcg gataaatttt
     8041 gtattgtgtt ttgattctgt catgagtatt acttatgttc tctttaggta accccaggtt
     8101 aatcaatcac agtttcatac cggctagtat tcaaattatg acttttcttc tgcagtgtca
     8161 gccttacgac gattatctat gagctttgaa tatagtttgc cgtgattcgt atctttaatt
     8221 ggataataaa atgcgaagga tcgatgaccc ttattattat ttttctacac tggctaccga
     8281 tttaactcat cttcttgaaa gtatataagt aacagtaaaa tataccgtac ttctgctaat
     8341 gttatttgtc ccttattttt cttttcttgt cttatgctat agtacctaag aataacgact
     8401 attgttttga actaaacaaa gtagtaaaag cacataaaag aattaagaaa atgcatcttc
     8461 cattcgcctg gataatatcg cttattacgg tcgttggagt tacgtgctgg atatgcaaac
     8521 ggtgctttga tatccggaat ctctcgcttc cgcctgggcc gccagacaac gtgcatgaaa
     8581 atgaacgcaa gtggatggct tactcacggt ggtccaaaga atcgggcatt caaggtcctc
     8641 tcgtcacact gtccacggca ccacacccaa tgatggttgt cagcggacgc acagtaatcc
     8701 gagatcttct gataggaaag aaatcgtttt gttgcaggcc acgcatacct atggccgagc
     8761 tcatgggtag acaaaacaat gttggcttca cgtactacgg aaagcgactc atcgcgatgc
     8821 gaaccctgct tcactccgcc cttagcctga ctgaagttac ttccacgtgg atggaactca
     8881 tcaactacca ggcggatgat ctttgtagag atttcattgt gtctccggaa cggttctacg
     8941 gtcatgttga agccaatata cagaaactca tcggacgctt tacttacggc cacgagccaa
     9001 gtcccgagta tctacaaatg gcggcccagg tgtcgcgaca cactgggaaa gctgtggagg
     9061 caggccgatg ggccgtgaac agtattccag ctcttatgtg ggtaccggca tggatgcccg
     9121 gtgctggttt caaaagatac gccattgatg caagaaagct gttcacgcgc ctgctcaatg
     9181 aatctgttgc gctcgtaaaa cagagaatag accaaaatga agcggacacg tcctttgtta
     9241 aacaatctct gaccaacatc tcgctatatc catcatgtat cgatgagtca cttgttatat
     9301 cttccgcggg gagcttgttt agtgcaggga cagagacgtt gtcaggtaca attctttcct
     9361 ttatcgtcct catggcaaac catgaagatt tccaagaccg tgcatatgca gaaattctgt
     9421 ctgttgtcgg cacagatagg tttccaaatt gtcgagaccg cccatcgctc atatttgtgg
     9481 atgcacttat ccgagaagta taccgattca atcctatcgt acccgtgata caccgtagca
     9541 actacgaaga ggaggaatat gaagaacatt ttattcccaa gaagacttgg atcctcgtca
     9601 acctgtggtc gattttacat gatcctaacg tctatccatc gccagaaaca ttctgtcccg
     9661 aacgttttct caactccgat aaagacatca atcccgatcc ccgctctttt gtttatggtt
     9721 ttggacgaag aaggtgtccc gcagtggatt tcgcggatac aacacttttt attaccataa
     9781 ctcgccttct gatggtcttc aaaatttccc cggagatcgt tgatgggatt gcatacccgc
     9841 ccctgctgga gattatggaa ggatttactc cggctccaaa accgttccga tgtaaattca
     9901 tatctcggca ggacagcaag cgcttgctat tcgatccatt tgacagtacc acagataatt
     9961 gaatttaact ccttaagtta ctttaatgat ttagttttta ttattaataa ttcatgctca
    10021 tgacatctca tatacacgtt tataaaactt aaatagattg aaaatgtatt aaagattcct
    10081 cagggattcg atttttttgg aagtttttgt ttttttttcc ttgagatgct gtagtatttg
    10141 ggaacaatta tacaatcgaa agatatatgc ttacattcga ccgttttagc cgtgatcatt
    10201 atcctatagt aacataacct gaagcataac tgacactact atcatcaata cttgtcacat
    10261 gagaactctg tgaataatta ggccactgaa atttgatgcc tgaaggaccg gcatcacgga
    10321 ttttcgataa agcacttagt atcacactaa ttggcttttc gctagtctta tctaaaaatt
    10381 gcctttatag tccgtctctc cagtcacggc ctgtgtaact gattaatcct gcctttctaa
    10441 tcaccattct actgtttaat taagggattt tgtcttcatc aacggcttcc gcccaaaaaa
    10501 aagtatgacg ttttgcccgc aggcgtgaag ctgcccatct tcacgggcct gacctcctct
    10561 gccggaacac cggccatctc caactcataa attggagaaa taagagaatt tcagattttc
    10621 agaggatgaa aaaaaaaagg tagagagcat aaaaatgggg ttcacttttt ggcaaagtta
    10681 cagtatgctt attacatata aatagagtgc cgataatggc tttttttcat cttcgaaata
    10741 cgcttgctac tgctcttcca gcgtttttat tacttctttc ttgtttctcc ttagtatata
    10801 aaatatcaag ctacaacaag catacaatca actgtcaact gtcaattata ttataataca
    10861 ctatgagtac ctctttaact aactacgctc ttccggacag taggatcgag gttgtgcggc
    10921 ttaaccagca atatatcagg ttcaagaaac ttttcggacg taccggacca tttccgacaa
    10981 ctgtcgacgt tgacggcatc gaccatgttc tcgaggttgc ggccggaaca tgtgcttgga
    11041 ctctggatat cgcacatctt caagaagtct acacccgcct cccatcgatg ccatcatcga
    11101 ctcgtcctga gtctacgtca aagccaattc agttgtacgc ctgcgatatt agcacagata
    11161 agtttccccc gagggacgtg acggatgcgc tcggtatcaa gacgtttcaa caggacgtta
    11221 cacaaccctt tcccgacgag ctttacggaa agtttgacct cgttcaagcg tttatgcttg
    11281 tcggttcttt aacagaggct gggtggagga gggctctgga gaactaccgc agacttctca
    11341 aacccggagg gtttctcttc atgctcgaaa cggatgtggc cctgtattca acgagcctcc
    11401 caccaccacc accaccgctt ttcccccttg acatcgacgg tgttatggac ggcctcacgt
    11461 ggattcacag tgcgaatcgg ttgttcaccg ggttcaacat aaaccgggac ctggttccta
    11521 atatatcgtc ccggttgccg gcgctgttaa cggagcagtc tctcaacatc atacaagaag
    11581 ctaaggctat ctcaccggtt ggaaagctct gcaaattata ccgcgcgttg gacggcgcag
    11641 atttatctga tgattgggat gactctgttc agggcactcg ggatgttttt cgtggattgg
    11701 ctcagtcaat gcttagaaga agcatgttgg aggtaccaaa aggaacaccc atcacgactc
    11761 aggaaggtct gaatgagctt ctcgcttgga tatttcaggg gttggaatcg gatggggctt
    11821 gtcatgtgat gacacagtgg ataataagaa ggaactgagc gaatttctta tgatttatga
    11881 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
    11941 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
    12001 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg cctctcaaat
    12061 cttttagcgc caaggactcc aactaattgt atcttgaatt tgcctttacg atccgtttgt
    12121 ccagtcacgg catgtatatc ttattaatcc tgcctttcta atcacgtatt ctaatgttca
    12181 attaagggat tttatcttca tcaacggctc ccacgcaaaa aatgacgttt tgcacacaga
    12241 cacgaaatac accttccacc ggaacaacgg ccatctccaa cttataagtt ggggaaataa
    12301 gacaatttca gacttcagag aatgaaaaaa aaaaaaggta catcacagat ggggttcagg
    12361 tttgctacaa ttgcagggag cctgtcacat ataaatagac ctccagtgat gatatctttc
    12421 agtcttcaaa cgtctcttgt cacagttctg gtcgttctat atcacatctc tcttggttct
    12481 acttattgtc tataatatca agctacagca agcatacaat caactatcta ccataccata
    12541 atacacaatg aactctgagg ataccgccgc tgaccatgga ccacccagta tctcgaaagc
    12601 tgaagaagga ttggaatcta ctgggccttc tggagaaacg cccgcttctg cgctcatctc
    12661 tgactttcct gaaggcggtc gcgacggatg gttgaccgtg ttgggaggtg ctcttgttct
    12721 cttttcttca ttcggtgccg ccctctcctt cggagtcttt caagactatt acactcgcat
    12781 ttctctaaat gaacactccg cgtcgcaaat atcctggatc ggatccactc agctgttcct
    12841 tgattttgct gtcggcctgc ccgcagggaa actcttcgac gaaggtcatt tcaggcttct
    12901 tatgatttca ggaactgtct tatacagctt ctcgttcttc atgctttcac ttgccaaacc
    12961 gcataactac taccaagtct ttctgtcgca ggccattggt atgggactcg gaatggggct
    13021 agtcatcatc cctgctgtct cgataccatt gcattacttt aggcggagga gattacttgc
    13081 tatgggattc atatttgcgg gctcctctct tggcggtgtc attttcccta tcatgctcaa
    13141 caagcttatc aacggccgtg ctgggttcgc gtggggaata agggctgccg ctttcttgtc
    13201 gctcgggctc ttgatcatcg caaatttgat tatgaagcct aggctacccc tcaaaaagga
    13261 tcgatcgcct gatactgtgg taccgttgaa gagcatattg accgatgtcg catacctaat
    13321 cagtgtcttt ggagccttcc tgattttttg ggggcttttc ttccctttct tctatctcca
    13381 gctgttttcg gtacttcacg gagtagacga aaagcttgct ttttactcgt tgacggttct
    13441 caatgcgtcc acaatctttg gtcgcacaat tccgaatttc gcatccgata aatttggacc
    13501 tttgaacgtt gcgatcccga tgactaccat cgccggcggg ctcgtatttg cgatgctggg
    13561 cgccacaagt gtcggcggaa tgttcacatt cgcagtcgtc tatggattct tcggtggagc
    13621 atgtgcctcc cgcatcggta tcgcaatgtt tgtctgcgga tttgcaatcc tcaccggaac
    13681 acccatcacc ggggcactgc tcaaaccgcc tcagtacaac acctggacga agcccgtcat
    13741 ctttagcgcg gtggtcatct tggctggggt cggatgcctg gtgatatcaa ggcagatgac
    13801 ggtgaagagg aaggggacgt ggaaagtgtg aggagattga taagactttt ctagttgcat
    13861 atcttttata tttaaatctt atctattagt taattttttg taatttatcc ttatatatag
    13921 tctggttatt ctaaaatatc atttcagtat ctaaaaattc ccctcttttt tcagttatat
    13981 cttaacaggc gacagtccaa atgttgattt atcccagtcc gattcatcag ggttgtgaag
    14041 cattttgtca atggtcgaaa tcacatcagt aatagtgcct cttacttgcc tcatagaatt
    14101 tctttctctt aacgtcaccg tttggtcttt tatagtttcg aaatctatgg tgataccaaa
    14161 tggtgttccc aattcatcgt tacgggcgta ttttttacca attgaagtat tggaatcgtc
    14221 aattttaaag tatatctctc ttttacgtaa agcctgcgag atcctcttaa gtatagcggg
    14281 gaagccatcg ttattcgata ttgtcgtaac aaatactttg atcggcgcta tgcggccgcc
    14341 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgag
    14401 actcaaacaa gatatatttt gcccgcctta cagacgaaac tagttggagg ttatggagca
    14461 tactatcacg tgggcggcca ctggtgagtt actacacccc aggggcaacg ttgatgctcc
    14521 taaaaaactc tggctggacg caagccgtaa cacccgtgtc acttcataat cgtttgcaat
    14581 tcagggcttg atctacacat taccctgtta tccctattaa ttaatacggt tatccacaga
    14641 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    14701 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    14761 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    14821 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    14881 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    14941 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    15001 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    15061 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    15121 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    15181 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    15241 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    15301 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    15361 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    15421 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    15481 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    15541 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    15601 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    15661 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    15721 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    15781 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    15841 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    15901 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    15961 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    16021 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    16081 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    16141 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    16201 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    16261 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    16321 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    16381 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    16441 ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg cttcattttg
    16501 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
    16561 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
    16621 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
    16681 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
    16741 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
    16801 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
    16861 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
    16921 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
    16981 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
    17041 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
    17101 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
    17161 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
    17221 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
    17281 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
    17341 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
    17401 tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag
    17461 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
    17521 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
    17581 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
    17641 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
    17701 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
    17761 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
    17821 actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    17881 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctcgg cgcgccatta ccctgttatc
    17941 cctaatatac aacgcaaaga agctggaaaa ttggcgaggg aatcctgttt ctgtctatcc
    18001 aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca gggtgcactt tgtaaccatt
    18061 tgggacaccg gacactcgct gttttcgaaa ttacccttta agcgcgggta ttgaaccagg
    18121 cttatgccca agatcgtagc aagcatttaa atgacggtat cgataagctt gatatcgaat
    18181 tcctgcagcc cgggggatcc actagttcta gagttaatta a
//
