LOCUS       sequence-21787-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Jin J, Cai Y, Yao T, Gottschalk AJ, Florens L, Swanson SK, Gutierrez
            JL, Coleman MK, Workman JL, Mushegian A, Washburn MP, Conaway RC, 
            Conaway JW
  TITLE     A mammalian chromatin remodeling complex with similarities to the 
            yeast INO80 complex.
  JOURNAL   J Biol Chem. 2005 Dec 16. 280(50):41207-12.
  PUBMED    16230350
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Dec 16. 280(50):41207-12."
COMMENT     Sequence Label: FLAG-hIes6
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(748..765)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    754..865
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagca tgccagacta caaggacgat gatgacaagc
      121 tcgagatggc ggcgcaaatt ccaattgtgg ccaccacttc cactcccgga atagtccgga
      181 acagcaagaa gaggccggcc agcccttccc acaatggcag cagcggcggg ggctatggcg
      241 ccagtaagaa gaaaaaagcg tccgcttcca gctttgcgca gggtatcagc atggaagcca
      301 tgagtgagaa taaaatggtg ccctctgagt ttagcacagg acctgtggaa aaagctgcca
      361 aacctttgcc atttaaggat cccaactttg tgcactctgg ccacggtggc gcagtagctg
      421 gcaagaagaa cagaacctgg aagaacctga aacaaatcct cgcttctgaa agggcattgc
      481 cgtggcaact gaacgatcct aactacttca gtattgatgc tcctccatcc tttaagccag
      541 ctaagaagta ttctgatgtt tcaggtctgc ttgccaacta cacagacccc cagagcaaac
      601 tgcggttcag caccattgaa gagttttcct acattcggag gctgccctct gacgtcgtca
      661 ccggctacct ggccctgagg aaggccacga gcatcgttcc ctgaggtacc gtcgagtcta
      721 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      781 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
      841 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctgg
//
