LOCUS       sequence-217870-       14552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14552)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU30.1-2.2
FEATURES             Location/Qualifiers
     source          1..14552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4121..4130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8987..9007)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8987..9005)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9344..9932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9424..9443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10103..10894,10895..10963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10726..10745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10964..11068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11095..12437
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12058..12105)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12478..12496
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12768..13172
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13185..14279
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14527..14543)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14527..14543)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgtcga
      541 atcatggacc tgacgaggcc atcgccatcg ttggcattgc ttgcaagctt ccacaagaag
      601 cctcgaccgt cgaacgcttt tggcaattcc tcctgcaagg acgaagcgca cattcaccat
      661 ggccagatga tcgcttcgga gccggccact atcatcccga caatgagcga gtcggaacac
      721 actcggtcaa aggggcgcat ttcttggccg agccgcccga atatttcgat gcaccctttt
      781 tcggcatcac caaaggcgaa gccatttgca tggaccctca gcaacgtctg gtgttggaga
      841 acgtctacca tgctctagag aatgcaggaa tcccactctc cgaagccaat ggatccaaca
      901 cgtccgtgta tgtgtcgggc ttcaatcacg actatctctc catcataaat gcggatgcgg
      961 agagcccttt gcgatatcgt gcaaccggat tgaccaactc cgtcctcagc aaccgagtct
     1021 cttggttttt cgatttcaaa ggtccaagca tgaccatcga tactgcctgt agcagctccg
     1081 tagtgacact tcatcaggcg tgccagagtc tacgtagtgg cgagagcgac atggccatat
     1141 ccagcggggt cacggtcttg gcttacccca acgatgtggc ctccatgtct tatcaaggat
     1201 tcctatcgca agacggccgg tgctttagct tcgatcaccg tgccaatggc tatgctcgtg
     1261 gcgagggagt cgggacgatc attttaaagc gtttcagtga tgccattcga gatggcaact
     1321 ctataagagc cgtcattcgg ggcactggtg tgaatcagga cggccgaact cctggattga
     1381 ccaacccgga tagtctggcg caggaaaggc tgatcaggac ggtctatgca agggcaggct
     1441 tgagtgtcaa caatacggcc atggtcgagg ctcacgggac tggtaccgcg gcgggtgacc
     1501 cgatcgaagc caatggaatt gcgagagcct tctcagctcg gccaaggaat ctccctccgc
     1561 tgtacattgg ggcttgtaag tccggcattg gtcatttgga gggcgcggca ggcgctgcag
     1621 ccatcatcaa gtctgttctt atccttgaga atgggatcat ccccccaaac gtcaatttcg
     1681 aaaaagtcaa tccgaagatc ccggcaacaa aatggcggct tcaattccct cttgaaccaa
     1741 cgccttggcc tacgatcggc ttgcgcagga tctctatcaa ttcctttgga gtcggcggta
     1801 caaacggcca tgttgttctc gatgatgctt ttcactatct ccaggaccga gggttgaccg
     1861 cgactcatta tacacgaccc ttgcctccga ccaacgcaga tctcgatctc gtaaacggcg
     1921 atagattcaa catcaccaat ggacatgtga atgggctcac caacaaccat cccaacagtg
     1981 ctggagatag caatgccctt gccggagact ggagggcttt cgatccaatc gagtgtccac
     2041 caaagctcgt tcccttttct gctttcgatg aagaaggctg cagtcgcgca gcaaaaacac
     2101 atgcagaatt cctccatgcc aggcccgaat cgatatccga ctccgaaagt ttgacctatc
     2161 tgaacgatct tgcccataca atgagtcggc gctcgaagct ggcctggcgg agtcacgtat
     2221 tagcaaggtc gctggtcgag ctcgagacac gtctggcttc gcccttgccc aaaccgctgc
     2281 gggctcgcac cactgtcaac gtggcctttg tgttcacggg tcagggagca cagtggtatg
     2341 gcatgggacg tgagctactc tgctacccgg tgttccgtga gagtctggct gcgtgcagag
     2401 tttatttgca gatcgaatgt ggctgcccct gggatctcct cgacgagctc gccaaagaca
     2461 aagaacatag tcgtcttgat caagcagcct tggctcagcc ggcgtgcact gcccttcaag
     2521 ttgcgattgt ggatttgttg caatcgtgga atatccagcc cagtcgtgtc gtgggccatt
     2581 cctccggcga gattgctgct gcttactgcg ctggccttct gactcgcgag tcggcctggc
     2641 gagcgtctta tttccgtgga ctagtcaccg ggaggcagac gaccgagacg aaaggcgcta
     2701 tgcttgctgt cgggttgggt caagaagcgc tcgcccctta tcttgaccga attcatgccc
     2761 aactctctgg ggaattagtt gtggcatgtc tcaacagtcc tcgcagcagc accgtctcgg
     2821 gtgatgtgca taaagtcgaa gctctgcaac aagtcttgga ggctgaaggg ctcttcgcga
     2881 ggaagctcaa ggtcccaaat gcctatcact ctgcgcatat gcaggaattc gcagacgaat
     2941 acgtccgtct ggtgggtacg ctccacgcgc cgagcgagcc acctcagtca cctgtggtca
     3001 tgatctcgag cgtcacgggg gaagcagtcg aaggccgttg tctctccatc gactattggg
     3061 ccaagaatct cctaaatcca gtgcgtttct ccgatgccct ggcaggcctt tgctttagcc
     3121 gcacaaccca aacacaggcc accctccgtg cagatgctgc tggagccaac gtctttgcgg
     3181 atgttttgat tgagcttggc cctcatggag cattgcaagg cgccataaga gatgtgctcg
     3241 cagctcatgc cgagggcgtt gcggtagcat tcctcccggc actgaaacgc aatgcccctg
     3301 gggcagacgt attcctgaac gccataggct accttcatgc ccgcgggtat ccgatcaacg
     3361 ttgatgacgt gaataacagc gctgatacga ctggtggaag agggtcccgc ccattgcgta
     3421 tacttcctga acttcctgga taccagttca accattccaa caagttttgg tatgagagtc
     3481 gcttaagccg gaactatcgc ctccgcaaag agatcagaca tgacctgttc ggtgctccag
     3541 tacccgactg ggacgcgatg gctcctcgat tccgcaactt tctgagaatc tctgagcagc
     3601 catggctacg agatcacatc gttactgata acatcatctt gcctggcgta ggctacgtga
     3661 ttgcggtaat cgaagcagcc agacagctgg ctgatccaaa tgtacaattg agcggcttcc
     3721 ggcttcgaga tgtcaacatg aaacgtgcat tgatcgtgcc cgacacgaaa gaaggggtcg
     3781 aagtaatgtt gaatttcaag ccagttgatg agtccagtgt cggcacttcg gcttattggc
     3841 gtcggtttca agtagtgtcc tataacccaa ccgcgaacga ctggatcgaa cactgcactg
     3901 gatacgttgg cgtggatcat gagacatctg cccccaatcc ggtcgaccag ggacgagaag
     3961 cccgacacga cgcagaacgc tggcacgccg ctttcactgc tgcagaagag cagtgtcaat
     4021 ctccctggga cttttccaag gcctatgagc gattggcaag aatcgggctg aactttggac
     4081 cgctgttcca gaactgctcg gctgtccggg gtacctttga ccatggtggc cagactatgg
     4141 gcacagtcac cgtccccgac gttgcaagtg ttatgcccaa gggtatcgta tctccgcact
     4201 tgatgcatcc agcttgcatg gacagcttga tgcactttgc gctggccgcg atcatggacg
     4261 ccacaggaaa gagcaacctc gaagtggcca tggtgccgcg gttcatcaag gacatttgga
     4321 tttctgcggc aatgaaccct gagcctggtc atgtctatcg tgctcacagc cagacgcgca
     4381 agattgcgtt tgagaagttc tcgacagatg ttgtcgtctg ggatggaccc actcaagaac
     4441 cccgtatctc ctggagtgga attgaagcaa gccccctgga ttccgccgca cagagctcgc
     4501 tcggcgcaga aaagatgtgt cacgagattg aatggattcc tcacgtggat atgtatctcg
     4561 aaggaggcga cgtcgcttcg ttcgctgaga tgcccaagcc tgatttggac tcagagcaag
     4621 ctgcgatcga gatcgattct cgtatgcagc tggcctcggt gcttcttgtc ctggacgcac
     4681 tggaggaact agatggcaag gtgccggccg ctgctcaagg tcatctctat cggtatttcg
     4741 agtggatgaa gcacgtcgag agtgatctcc agctcggcaa ggtgatcggt atccaagcct
     4801 ctgaggttga gcgactcaaa catgaccacg ctgcaaaggt ggagctatac aaacaggttg
     4861 cttcggacac tgcgttcggt gagttggcag tacgaatggg cggcaacatc gtgcccgtct
     4921 taagaggtga gacagacccg ctgcagctca tgttcgctga ggatgccata ctcgatcgag
     4981 tctacagacg aactgcgacc ttgaacgacc tgccagcgca acagaaggcc tatttgaacg
     5041 tattagccaa gaataaggcc aatttacgcg tgctcgaggt gggtgccggc actggctcgt
     5101 ccactgcgca agtacttgat gcccttgctc ccatcgcaga tgatggggtg tccattgcta
     5161 gtagcgtgtc acattacatg tacactgata tttcggccgc ctttttcgaa caagcaaggc
     5221 agaaattcca gccatacacg aacatcatgg agtatcgagt tctggacgca gaacaagaca
     5281 tcgccaagca ggagagcttc gaagtcagga gctatgattt gattgtagct cagaatgtca
     5341 tccataccac caccgacctc accaaaacac tgagtaatct tcgccagttg ctgaagcccg
     5401 gaggccgcct gcttctacaa gaaggtataa ggcaggatta ctactggtcc gggctatcat
     5461 ttggccagct gtcagggtgg tggagtggca ccgaatccat gcgtcaatgg atgccatggg
     5521 tatcatccaa gcagtggaat gaagtcctcg aggctgctgg ttttacagga gcgcgcttcg
     5581 aacttcccga tagccacaat gaacagctcc atagccagag tcttttcgtg gcccagaacg
     5641 cttctgtaga cgccaacgag cccggtccca tgtggaagaa cgttgtgctc gtcacactcc
     5701 ctagcttcga gcgaagcaac actcaaagct tgttgcaaga catcaaagac aagttgaacg
     5761 agaatctccg gttccagaac atcaggatcg tatccctgct agaattggaa gggatcgagt
     5821 atagccaaag tctctgcatt gttctagcag aactcgaacg tcctgtgatt gcagatctgt
     5881 cccaggccga gtacgagggt gtccgcaaaa tgctgatcga gactaaaagc atcttgtggg
     5941 taactggaga tgagcttttc tgcccccagt acggagccat aaccggcctg atgcgtacaa
     6001 acagatggga acgtgacttg gacgattcca acctggtcac actgaaaatc tctgagcctc
     6061 gacctgaaaa tacctcgctg agtgcgtcga ttcgaaggct gtgtgaggcg cagttctcgc
     6121 tcactctccc agttgagaac atgaatggcg agttcatgct tcaagatggc cggatctgga
     6181 cgagtcggct ccgcgaggcc attcgtgcag atgaatactt gcaaacagct ctataccgct
     6241 cagaagcagt cccaacgctg gccagggatg ctggcaggcc catcaagctc acgacaactg
     6301 ttccaggcct gctagaaaaa cttgagtggg tgaccgacga gacgtacaat gagccactct
     6361 ccgatacaga agtagaaatc gagatacgag ctgttggcct caactttcgc gatctcatga
     6421 ttgccatggg ggagcatacg gcgtactcga tgggcagtga agcagctggc atcatctctc
     6481 gcgtgggctc caaagtagtc gacttcgctc ctggcgacag ggttgtctac atctgtggcc
     6541 tagaccatgt cgggtgcatg cacacctatg gtcgtttgga tcaatcgaca gtcgccaagg
     6601 tccccgatgg gctcagtttg gagatggctg ccggcttgcc tgttgtctat gcgacagtca
     6661 tttacagcct acgggagacg gctcggttga tgcccgacga aaccattctt atccatgctg
     6721 ctgccggtgg ggtgggccag gctgcgattc gttacgctca acatattggc gccgaggtgt
     6781 atgtaaccgt atcgactacg ttgaagcgag acttgataat tagggagttc gggattccag
     6841 acgatcacat cttttcaagc cgcgacctca cattcgtccc gggcgtgttg cgtatgacaa
     6901 aaggcagagg tgttgacgta gtcttgaatt cgctctctgg tgaggcgctg cgccgctcat
     6961 gggatctggt ggcacccttc ggtcgtttca ttgagattgg gaagaaagac gccatgatga
     7021 atggcaaagt ggatcttcgg ccctacttgc gcaacgtgac catggcgagc gtcgacctcg
     7081 tttctatgat gaagaacaaa ccgtttctca tcaagatgct gacagaggaa acgatgcggc
     7141 tgtggaaaga gggtgtggca ctgcctgcat caccaactac gataatgccc atgtcgcagg
     7201 tagtggatgc acttcgcatt ctccaggccg gcacgggaat gggcaagatt gttctggtgc
     7261 ctcgggagga tgacgttctg ccactccttc caccgtcacc ggcgccgctt ggcttttgtc
     7321 gagatgcatc ctacgtgctc agtggtggcc ttggaggtat tggacggagc gttgctgcat
     7381 ggatggcttt gcgaggcgct cgaaatttga tcttcctgtc cagcagcggc cgcatcactc
     7441 cagccgtcac aacgatgcgc gagactctcg aagcagacgg atgtcgtgtg catatcttca
     7501 catgcgatgt cagcgacaag gaacagctac gtctcgttct cgaacaatgc aagaccctcc
     7561 cacctataag aggagtcgtc caagcagcaa tgaagctaaa ggacatcatg ctcgagaaca
     7621 tgtcgcacga ggaattccag ctggctatca gacccaagat tcaaggttcg tggaacctac
     7681 acgaacttct gccccaggat ctggagcatt tcatcatgct gtcttcagcc acgggcgtcc
     7741 tcggcaaccg ggctcaggcc aactacgccg caggaaacac cttccaggac gcgctggcgt
     7801 acttccgtcg gcagcaaggt ctagcggcca cgacgatcga cgttggcgca gtgctagacg
     7861 tcggatacgt ggccgatcac gcggaccgcc tcgccatgac aaaataccta ggcagtatga
     7921 tgaaagtgct gcgcgaagaa gagttgctta cgcttatcga gtacggcatg aacgctgctc
     7981 tgcagtcgcc tgcacagctt gttacgggct tgactccgtt agacaagcat cgtgctcgag
     8041 gcgtgccgat gctcagttac atgaacttcc ctctcttcac gcagctgcgg cggctcaata
     8101 cgcagcaaga cggcgcaggg acgaccgggg gtgatggacc tgacgttgaa gcgcggcttc
     8161 gagcagctcg gactctggat gaggcggctc agattgtgac ggaatcggtc attgataagt
     8221 tgtcttcctt gctctcaatt gcggtcgagg atgtggatcc ggcgcggacc atcagtgcca
     8281 atggcgttga ctcgcttgtt gcgctagaac tgaggacgtt catggcgaga aaggtcaagg
     8341 cggatgtgcc tgtgttggaa atcatgggct cgttgagtct tgctcagctg tgtaggaagg
     8401 tggcatcgac gagcaaagcg gtcgagttgc taatagcagg aggcaactaa ggagattgat
     8461 aagacttttc tagttgcata tcttttatat ttaaatctta tctattagtt aattttttgt
     8521 aatttatcct tatatatagt ctggttattc taaaatatca tttcagtatc taaaaattcc
     8581 cctctttttt cagttatatc ttaacaggcg acagtccaaa tgttgattta tcccagtccg
     8641 attcatcagg gttgtgaagc attttgtcaa tggtcgaaat cacatcagta atagtgcctc
     8701 ttacttgcct catagaattt ctttctctta acgtcaccgt ttggtctttt atagtttcga
     8761 aatctatggt gataccaaat ggtgttccca attcatcgtt acgggcgtat tttttaccaa
     8821 ttgaagtatt ggaatcgtca attttaaagt atatctctct tttacgtaaa gcctgcgaga
     8881 tcctcttaag tatagcgggg aagccatcgt tattcgatat tgtcgtaaca aatactttga
     8941 tcggcgctat gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     9001 taattgcgcg cttggcgaga ctcaaacaag atatattttg cccgccttac agacgaaact
     9061 agttggaggt tatggagcat actatcacgt gggcggccac tggtgagtta ctacacccca
     9121 ggggcaacgt tgatgctcct aaaaaactct ggctggacgc aagccgtaac acccgtgtca
     9181 cttcataatc gtttgcaatt cagggcttga tctacacatt accctgttat ccctattaat
     9241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10201 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11041 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa
    11101 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
    11161 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
    11221 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
    11281 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
    11341 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
    11401 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
    11461 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
    11521 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
    11581 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
    11641 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
    11701 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
    11761 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
    11821 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
    11881 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
    11941 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
    12001 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
    12061 gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa
    12121 acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca
    12181 cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt
    12241 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc
    12301 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt
    12361 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt
    12421 tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac ctataaaaat
    12481 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctcggc
    12541 gcgccattac cctgttatcc ctaatataca acgcaaagaa gctggaaaat tggcgaggga
    12601 atcctgtttc tgtctatcca agaatgggca tgaggtggca accgtcgtgc tagcgtacag
    12661 ggtgcacttt gtaaccattt gggacaccgg acactcgctg ttttcgaaat taccctttaa
    12721 gcgcgggtat tgaaccaggc ttatgcccaa gatcgtagca agcatttaac tgtgggaata
    12781 ctcaggtatc gtaagatgca agagttcgaa tctcttagca accattattt ttttcctcaa
    12841 cataacgaga acacacaggg gcgctatcgc acagaatcaa attcgatgac tggaaatttt
    12901 ttgttaattt cagaggtcgc ctgacgcata tacctttttc aactgaaaaa ttgggagaaa
    12961 aaggaaaggt gagaggccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta
    13021 aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc
    13081 aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat
    13141 atatatatat atttcaagga tataccattc taatgtctgc ccctatgtct gcccctaaga
    13201 agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg
    13261 ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa
    13321 ttggtggtgc tgctatcgat gctacaggtg tcccacttcc agatgaggcg ctggaagcct
    13381 ccaagaaggt tgatgccgtt ttgttaggtg ctgtggctgg tcctaaatgg ggtaccggta
    13441 gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact
    13501 taagaccatg taactttgca tccgactctc ttttagactt atctccaatc aagccacaat
    13561 ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta
    13621 agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag
    13681 aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc
    13741 ctatttggtc cttggataaa gctaatcttt tggcctcttc aagattatgg agaaaaactg
    13801 tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa ttgattgatt
    13861 ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca
    13921 acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt
    13981 tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac
    14041 catgccacgg ttctgctcca gatttgccaa agaataaggt tgaccctatc gccactatct
    14101 tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg
    14161 aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca
    14221 acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa
    14281 aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa
    14341 acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc
    14401 tacatacatt tatcaagaag gagaaaaagg aggatagtaa aggaatacag gtaagcaaat
    14461 tgatactaat ggctcaacga aatgacggta tcgataagct tgatatcgaa ttcctgcagc
    14521 ccgggggatc cactagttct agagttaatt aa
//
