LOCUS       sequence-217872-       12011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU34.1-2.2
FEATURES             Location/Qualifiers
     source          1..12011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(714..725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6446..6466)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6446..6464)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6803..7391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6883..6902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7562..8353,8354..8422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8185..8204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8423..8527)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8554..9896
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9517..9564)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9937..9955
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10227..10631
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10644..11738
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11986..12002)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11986..12002)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggagg
      541 ttgtatattt cagtaatgag ttccctaaag atgatctctc ggagatcttc agacacttgc
      601 acaaccaaag caagaccacc gagcacgaaa ttctagcccg cttcctcaga gacgcaacaa
      661 gagcagtcaa gaaagagatt gaaagactcc catcaacttt gaaaaaccag atccctacct
      721 tcgatactct catctcttgg gtagagaaga aggagctccg cgagggtgaa ctatgtggcg
      781 cggtcgatgg agttctgctg atactcgttc aactcgcggt ttacatattt aatgctgaaa
      841 gcagtaatga ttcaacattc tcgcacgatg aaaagcacct cacgggtctg ggcgtcggct
      901 tgctttccgc taccgcggtt gctctagctc gagacatttc tgaactagct ttctacggtg
      961 tgcaggcgat cacagtcgcc ttccgcatgg gagtccatgt acaaggcgtt tcacattatc
     1021 tagaagctcg cgacccggct gaacccccaa agacatgggc ttatgtggtc cataacgttg
     1081 atccccaaga gacgaaaaag gagctagaag cataccacga agcagtgccc agttcggata
     1141 ccagtcgtat cttcatcagc gccatcagcc gaacatctgt cacaatcagt ggcccacctt
     1201 caaaacttga aaatctcttc aagaaggcca cagcattcag agacgccaag tccattgcac
     1261 tgcctgttta tggaggtctt tgtcatgctc cccacatata cgactctcac gacatcgagg
     1321 ctattgttca agacgttgtc agttcatcgg atcgacttgt ttcgcaaaac gaaccaagat
     1381 ttccactttt ctcgacaagc tccggtactc catacatatc atccagcccc gcagaactga
     1441 tcaagagcgt tgtagcagag ctcttgacaa aggctattga gtgggacaaa gttattgcac
     1501 aggaggttga atacgctaag cagaacaacc gaactcaaat caggctgtct tgctttggca
     1561 actccattcc attgaatgat ctgcgttctg agttgcaaaa gacatccaag gatacttcta
     1621 tttccttgaa cacctacatg tcggcgcttc tcaaggatgc tactgagaat gtaacccccc
     1681 gaggtccagc acaatccaaa cttgccattg tgggaatgtc ttgcaggctt ccaggcggat
     1741 caacggatac cgagaaattc tgggaagtgc ttgaagcagg gcttgatgtg tctcgccaga
     1801 tacctgcaga ccgattcgac atcaatacgc actatgaccc tgaaggcaag gatctcaaca
     1861 agacaatgac gcaatacgga tgcttcatcg aagaaccggg aatgttcgac ccaagtttct
     1921 tcaacatgtc acctcgagag gcgatggttg ttgatcccca gatgcgactc tcgcttgtca
     1981 ctgcgtatga ggcattggaa cgggcaggct acgttgccaa caggacccag tcgaccaggc
     2041 tccagcgcat cggaacatac tacggccaag cagctgacga ctatcgcgag gtaaaccaag
     2101 gacaggaagt cggaacctat tacattccag gaggatgccg tgcttttgga cctggtcgaa
     2161 ttaattactt cttcaaattt gccggtccca gctacagcat tgacaccgcc tgctcctcag
     2221 gactcgcggc tatcgaagtc gcttgtcaag ctctttggag tggtgaagtt gacactgctg
     2281 tagcaggagg agtcaacatc cttacgaacc ccgatggttt tactggtcta agtaagggtc
     2341 acttcctaac caagggccac aacgcctgca aaacttggga cgctagtgct gatggctact
     2401 gtcgcgctga tggcgtggga tcgattgtca tcaagaggct cgaagatgca caagctgata
     2461 aggataacat tcttggtgtt atccttgctg caggcaccaa ccattcggca gaggctgtct
     2521 ccatcaccca tccccatgca ggacatcaat catatttgtc gcgccagatc attaggcagg
     2581 caggtattga tccgctggac gtttcatatg tagagctcca cggtaccggc actcaagctg
     2641 gtgattttga ggagatgaag ggtgtcctag acgtgtatgc tccaaacacc tttcccaacc
     2701 gcaggtctga tcagaagcta cacattggaa gcgccaagtc aaatgtcggc cacggagaat
     2761 cagtggctgg gactacagca cttatcaagg tcttgttgat gctgcagaag aatgccattc
     2821 ctccacacat cggcatcaaa acagaggtta acccacgctt acctaaggat ctggatcagc
     2881 gaaatgtcgc aatcccgttc tccatgaccg aatggaagag tgatggcaag aagaagcgta
     2941 tcgcggctgt taacaacttc ggagctgctg gcggcaacac tatgatgctt ttggaggaag
     3001 cacctccagt cgtggtggca cgcgagaagg aaaccaggcc aacacatgtc atcgccgtgt
     3061 ctgcgaagac caagacctct ttgcgcaaca acatcaaccg tctccttgtg tatctcgata
     3121 gaaaccctga tgtctccatt tcggatctcg cctataccac gactgcgcgc aagtaccacc
     3181 actcgtacag ggtcgcagtt agtgctacgg atcttgcttc agtgaagaag caactgtcaa
     3241 cacagcttaa caaggttgat tccttgaaac ccttgaacaa aaccaacccg ccgtcaatgg
     3301 catttgtttt cactggccag ggcgcctcgt accagtcgat ggatctcgaa ctgtaccacg
     3361 aagcaccagt cttccgagag cacattcaat atctcgacag acttgctcaa gcccaaggct
     3421 tcccttcatt catcccagcc ttggatggct cccacccacg cgaccacgag caccctcctg
     3481 tcatcaccca actagccctg gtttgtctcg agattgcgtt agcccactac tgggcttctt
     3541 tgggcgtcaa acctgatgtt gtcatgggac actccctcgg cgagtatgca gctatgcatg
     3601 tcgctggagt catcagtgcg aacgatgcca tctacatggt tggttgtcgg gcactgatgc
     3661 tccagaagtc atgcactccg ggcagccact gcatgatggc tgttcgcgcg tctctcgagt
     3721 ctatcatcgg cgcatcgaat ggcaagccac acactgtggc ttgtatcaat ggtcccactg
     3781 acacggttct aagcggcacc aaggcacaga tggatgacat cgctgtagat ctcgaggcag
     3841 ctgggttccg ttgcatcaag cttaacgtgg catttgcatt ccactccgaa caggtcgacc
     3901 cgatcctcga cgagttcgag acgattagca agagtggtgt tattttccac gagcccaagc
     3961 ttcctgttgt ctcacccctt ctcggcaagg tcgtctttga cggacggtcg cttaacgcca
     4021 gctacgtgcg aaatgctaca cgagcaacgg ttgacttcct gtccgctgcg aagaacgctt
     4081 gcgatgtgtc aatcatcaat aaagagactg ttttcatgga gatgggtcct caccctgtct
     4141 gtgtcggatt catcaagtcc attttcccat cgacaaagac cgccgtcccg tccattcgcc
     4201 gtggagacaa caattggaag accatctccg agagcgcagc agctattcat ctcgatggcg
     4261 ttgatgtgga ctggaatgag ctccaccgtc cttttgcgga tgcagtgcgt cttcttgatc
     4321 ttcctacata tgcttgtgat gagaagaact attggataca atacaatggc gattggtgct
     4381 tgaccaaggg caataccttc tacgacaaga aggtcgaggc ggcagagcct gcagttggca
     4441 ataccaagaa atatgagtct ttgagctctt tggtacagaa catcatttca gtagatgtga
     4501 atggagacca gggcactgtg atcatgcagt cggacttaat gcagaaggac tttttggctg
     4561 ctgcccatgg acactcgatg aacagctgtg gtgttgtcac atcgtccatc catgccgaaa
     4621 tcgcatacac tctgggagag tatctctacc gcaagttcta cccaaagaca aagcgagtgg
     4681 acatgaacat gagcaacttg cacgtaacca gtgccctgat cgccaagaaa cactccatgg
     4741 acactcccca gtccatccag gtcactgcca gtaccaacga catcaagagt ggtactcttg
     4801 acttgacatg gcagaatgtc gaagaagatg gcagcctgtc ggagccattc gcaacagcca
     4861 atctcgagta tggcaacgca gacgattggc tgtcgagttg gagtcccatg gcacatctag
     4921 tccagcaccg tatcgagtct ttggagcagc tcgctgccga gggcaaggcc aaccgcttct
     4981 ctcgcaacat ggcctacacc ctcttcgcaa agaacctcgt cgactacgcg gacaagtacc
     5041 gcggcatgca atccgtcgtc atgaacgatc tcgaagcctt cgccgaagtg aagctcagcg
     5101 acaaagtcga caacggaacc tacaccgtac ccccctactt catcgacagc gtcgcccatc
     5161 tcgccggctt catcatgaat tgctccgaca ccatcgacac cacaaataac tactgcgtga
     5221 cctcgggctg gaaaaccatg cgcttcgccg agccccttac ccccggcgct cgataccgct
     5281 cctacgtcaa aatgacccca accaaggacg acccctccgt ctacctcggc gacgtcttcg
     5341 tcctcagcgc agcagacaac tccattgtcg gcatggtcgg cggcatccac ttccgccgct
     5401 tcccacgcgt cctcctcaac cgcttcttca ccgcgcccca gcccaccaag ggcagcgctc
     5461 aacccgccaa atccgccgca ccacccgctc ccagcaagcc ctccgcacca cctaaagtgc
     5521 aagcggttca ggcagcaccg gtcgtacccg tcgctcctag agaagacgac acccagtcct
     5581 ccgccagcga cacgtccgac cgcgataccc cctccgaagc ctcagtcaac acatccgcta
     5641 ccagcgtcga tgtgtctgct tctgcgcccc gcgctgctaa caccactgtt gacaaagcta
     5701 tcgatcttat cgccaaggaa acgggtatcg acgctgctga gctgaccgac gatgcagagt
     5761 ttgccaactt gggtattgat agtctcatgt cgctggtgtt gtccgagaag ttccagaacg
     5821 agctgagtgt caaggtgaat ggaagtttgt ttttggatta tccgacgatt ggggatttga
     5881 gggagtggtt ggagaagtat tatgcatgag gagattgata agacttttct agttgcatat
     5941 cttttatatt taaatcttat ctattagtta attttttgta atttatcctt atatatagtc
     6001 tggttattct aaaatatcat ttcagtatct aaaaattccc ctcttttttc agttatatct
     6061 taacaggcga cagtccaaat gttgatttat cccagtccga ttcatcaggg ttgtgaagca
     6121 ttttgtcaat ggtcgaaatc acatcagtaa tagtgcctct tacttgcctc atagaatttc
     6181 tttctcttaa cgtcaccgtt tggtctttta tagtttcgaa atctatggtg ataccaaatg
     6241 gtgttcccaa ttcatcgtta cgggcgtatt ttttaccaat tgaagtattg gaatcgtcaa
     6301 ttttaaagta tatctctctt ttacgtaaag cctgcgagat cctcttaagt atagcgggga
     6361 agccatcgtt attcgatatt gtcgtaacaa atactttgat cggcgctatg cggccgccac
     6421 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgagac
     6481 tcaaacaaga tatattttgc ccgccttaca gacgaaacta gttggaggtt atggagcata
     6541 ctatcacgtg ggcggccact ggtgagttac tacaccccag gggcaacgtt gatgctccta
     6601 aaaaactctg gctggacgca agccgtaaca cccgtgtcac ttcataatcg tttgcaattc
     6661 agggcttgat ctacacatta ccctgttatc cctattaatt aatacggtta tccacagaat
     6721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7201 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7561 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7681 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7861 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8041 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8401 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8521 ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta
     8581 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     8641 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
     8701 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     8761 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
     8821 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga
     8881 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt
     8941 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag
     9001 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag
     9061 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga
     9121 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt
     9181 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta
     9241 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa
     9301 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat
     9361 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg
     9421 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg
     9481 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa
     9541 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca
     9601 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg
     9661 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat
     9721 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc
     9781 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac
     9841 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac
     9901 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9961 cgtctcgcgc gtttcggtga tgacggtgaa aacctcggcg cgccattacc ctgttatccc
    10021 taatatacaa cgcaaagaag ctggaaaatt ggcgagggaa tcctgtttct gtctatccaa
    10081 gaatgggcat gaggtggcaa ccgtcgtgct agcgtacagg gtgcactttg taaccatttg
    10141 ggacaccgga cactcgctgt tttcgaaatt accctttaag cgcgggtatt gaaccaggct
    10201 tatgcccaag atcgtagcaa gcatttaact gtgggaatac tcaggtatcg taagatgcaa
    10261 gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg
    10321 cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc
    10381 tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg agaggccgga
    10441 accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt
    10501 gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc
    10561 ttactttcta acttttctta ccttttacat ttcagcaata tatatatata tttcaaggat
    10621 ataccattct aatgtctgcc cctatgtctg cccctaagaa gatcgtcgtt ttgccaggtg
    10681 accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg
    10741 ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg
    10801 ctacaggtgt cccacttcca gatgaggcgc tggaagcctc caagaaggtt gatgccgttt
    10861 tgttaggtgc tgtggctggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt
    10921 tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat
    10981 ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg
    11041 ttgttgtcag agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg
    11101 atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa
    11161 tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag
    11221 ctaatctttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg
    11281 aattccctac attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta
    11341 agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct
    11401 ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct
    11461 ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag
    11521 atttgccaaa gaataaggtt gaccctatcg ccactatctt gtctgctgca atgatgttga
    11581 aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt
    11641 tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg
    11701 atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat
    11761 atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg
    11821 gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg
    11881 agaaaaagga ggatagtaaa ggaatacagg taagcaaatt gatactaatg gctcaacgaa
    11941 atgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta
    12001 gagttaatta a
//
