LOCUS       sequence-217874-       12035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU35.1-2.2
FEATURES             Location/Qualifiers
     source          1..12035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6470..6490)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6470..6488)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6827..7415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6907..6926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7586..8377,8378..8446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8209..8228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8447..8551)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8578..9920
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9541..9588)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9961..9979
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10251..10655
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10668..11762
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12010..12026)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12010..12026)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgtccg
      541 tacatacccc aaccacggat tcggaatcct acgagggttt tcgcaagatg gagattgtct
      601 attttagcaa cgacttccct aaagatgact tggttgacgt ttttcgccgc ctacacaacc
      661 atagcaagga caagcatttc cctgtcttgg cccaattcat acgtgaggcc acctgggcta
      721 tcaaggacga agttcgccaa ctaccaactg agctcaaaca actgattccg ccattcaata
      781 ctctattggc ttgggcggaa aacagccagc ttcgggaagg cctaatctgt ggggctgtgg
      841 atggagtgtt gctcgtcgtc gctcagctag caacttacat tggatatgtc gagagtcgct
      901 ctggagattt gtttgacttt tcaattacat ctcttgctgg cctaggcact ggcctactat
      961 cgtcgagtgc cgtctcattg tcgcaaacat tggccgactt gccactggca ggcgcagatg
     1021 cagcccgtct cgccttccgc ctgggcattc atgtcttaga catgtcggag aacctggaag
     1081 ctcgtgattt atcagaaagc ccagattcgt gggcgtacgt ggttcacaat gttgacccga
     1141 ctacggccta caaggagcta gatgacatgc attctcgaat ggagataccc aatactggca
     1201 aaatatttat cagcgctgtt agccggacat cagtcacagt tagcggacca ccatcgaggt
     1261 taaaagcgtt gtttaataag tcagagttat tccgcgagtc gaaggtcgtt gagctgccag
     1321 tgtacggcgg cctctgccac gcgccgcata tcttcggtct ggaccatgta catagtatcg
     1381 ttcacggctg ttcattaaac gccgctatca caaattctcc gccagttatt ccagttttat
     1441 cgaccagcag cggtttgcca tatctggcca agagttccat ggagatgttt gaatgcgtcg
     1501 tctcagagct gctcacgcaa acaatctatt gggataatgt tgtcagtggc atagtcgaac
     1561 gagccaagtc taacgttacc gcggaagcag tgcttcactg cttcggcaac tccactcccc
     1621 ttaacgacct caatatgacc ctgaagagca atgttccaga tctcgacgtc tcgatcaaca
     1681 gtcttatgtc atggatctct caggtttcac caagagatac tacaccccac ggcactgcac
     1741 agtctaagct ggccattgtc ggtatggcct gcagatttcc aggaggtgcc acaaatactg
     1801 agaagttttg ggagatccta gagcaaggcc ttgatgtctc acgtcgagtt ccagctgacc
     1861 gctttgacat cgacacacac tacgacccaa ccggcaagca gcagaataag accatgacac
     1921 agtttggctg ctttattgat gagcctggtt tgtttgatgc gcctttcttc aacatgtctc
     1981 ctcgtgaatc tcaggccgtc gatccgcaga tgcgtttggc tcttgtaacg gcatacgagg
     2041 cactggagca agctgggtac gtcggtaatc gcacagctgc tacacagttg caacgcattg
     2101 gaacatatta tggacaagcc gccgacgact accgtgaggt taaccaaggc caggatgtag
     2161 gcacatacta tattccaggc ggttgccgtg cgttcggccc cggtcgtatc aattactttt
     2221 ttaaatttgc gggtccaagc tacagcattg atactgcttg ctcttccgga cttgcagcga
     2281 tcgaggtcgc ttgtcaggct ctttggaacg gcgatgtaga tatggctgtc acgggcggtg
     2341 ttaatgtgct aactaacccg gatgttttcg ctggcctttg caagagccat ttcctaacta
     2401 aaggtcacaa cgcatgcaag acctgggatt ctaccgctga cgggtactgt cgtgcagacg
     2461 gcgttgggtc tgttgttatt aaaaaactcg aagatgctga agcagacaac gacaacatct
     2521 tgggcgtcat tctcggtgcg ggaaccaacc attctgccga ggccgtctct attactcacc
     2581 ctcatgccgg ccatcaggcg tatctgtctc gtcaggtgct tcgccaggcc ggcgtggatc
     2641 cattggatgt ttcctatatt gagctccatg gcactggtac tcaagctggt gaccacgagg
     2701 agatgcaagg cataatggaa gtgtatgctc cgctcatcaa gcgacgtagg aaggaccagc
     2761 ccctacacat tggtgctgta aaagctaacg ttggccatgg tgaatctgtt gctggcacaa
     2821 cggcattgat caaggtgctt atcatgctcc aaaaaaaatc tatcccgcca catatcggaa
     2881 tcaaaacgga gatgaacccc agattccccc gagacttcga caaacgcaac ctccacatcg
     2941 acttggaaat gacaccctgg ccgcaaacat cgtctaagaa gcgacttgca gttgttaata
     3001 actttggtgc cgctggcggg aacacgacta tggttttaga ggaggcacca actcgagaca
     3061 tatccgaggc cgatccacgc aatactcata tcatcgccat atccgccaag actaaagggt
     3121 cattaacagg aaatattgag cgcctgatcg cccaccttga tgcgaatcca gatatcaacc
     3181 ttgccgacct ttcctacaca acgactgcac gtcgctccca tcacagccat cgtgtagcaa
     3241 ttgccacttc tgatataggc cacctaaaga agcagcttac ttcccgccta gaaaatatcg
     3301 attcaatgaa gcctattgga aattctggta gtccaccagt agctttcgcc tttactggcc
     3361 agggctcttc ttataaatcg atgaaccttg agttgtacca cgatgtacca acattcagag
     3421 agcatattca gcatcttgat tctctcgctc gacgccaagg ctttccatct attattcccg
     3481 ctctagatgg ctctcatcct aaggaccact tgcactctcc ggtagtcacc cagcttgccc
     3541 tggtatgcac cgagattgct ttggcaaaat actgggtttc tcttggaatc aagccagacg
     3601 tggtcatggg tcacagtctg ggcgaatatg ctgcgatgca catagctggt gtggttactg
     3661 caagcgacac catattcatg gttgggcgtc gtgctcaaat gctagaagaa aagtgcacga
     3721 ttagcagtca tagtatgatg gcggtccggg ctactcttca gcaaatagag gaatgcttag
     3781 gaacaaagcc gtatacgatt gcttgtgtca atggaccttc cgatactgtc ctcagcggta
     3841 ctaaggagag tacagatgat gtcgctgtgg tgctcgaggc ggccggttac cgttgcttca
     3901 agttggacgt cgcgtttgct ttccactcag agcagaccga tcctattttg gatgacttcg
     3961 aggagatatc aaagactgga gttgtttttc aggaacctaa acttcctttc atctcgccat
     4021 tgctcggaaa ggttattttt gacagtaaga cgctgaacgc aaactacgtt cgccgagcca
     4081 ccagggagcc tgttgacttc ttgtctgctt tgagaaatgc tcaaaagatt tctaccatta
     4141 gcgatgaaac tgtctggatc gaaattggac cacatcctgt ctgcactaac tttgtcaaga
     4201 ctaccatccc gtccacgaag cttaccatcc catcgatccg tcgtggagat aacaactgga
     4261 aaaccatggc tgaaagcatg gctgctctgc atcttgctgg gtttggggtc agttggaacg
     4321 aattccaccg cccatttgag caaaggctgc gccttttaga cttgccaaca tacgcctgga
     4381 atgacaagaa ctactggttg cagtataatg gtgactggtg tctgactaag ggcaatactt
     4441 tctacgatgc cgagaaggag gctgccagag caaaatctgt actaagatcg ctgcctgcga
     4501 gcgggtttct gacaacgacc gtgcagcaga tcatcgacga gtcttttagc gattttgctg
     4561 gcacggtggt gatgcagtct gatcttatgc aagcggatat gctcgccgct gctcatgggc
     4621 acagcatgaa caattgcggt gttgttacat cttccatcca cgctgacctc gcctacaccc
     4681 tcggcgacta tatataccgc aagttctatc ccaaagccaa ggaagtcaat gttaactttg
     4741 ccaacttggt tgtcgcaaaa ggccttgttg cccaaaacag caacaagact ccccaattgt
     4801 tccgtgtaac tgctgtcact gccgacgtcc gctcaggcgt ggtcgactta acgtggcaga
     4861 atgttgacaa tgatggctat gcccacgagc catttgcaac cgccaacata atctatgggg
     4921 attcatctga atggatttca tcttgggcct cattaggtca cctcatccac ggccgcatcg
     4981 aagcgcttga gcgcctagct acagagggaa aagccaaccg tttctctcgc aaaatggcat
     5041 acacgatctt tgccagtaac ctagtcaact acgctgagaa ataccgtggc atgcaatctg
     5101 tggtgatgca tgaactagaa gggtttgctg acgtgcaact gaccacaaag gagagtggag
     5161 tctggacagt tccaccatac tttatcgaca gtgtggtgca cttggctggc tttatcatga
     5221 actgctcgga tgccattgat acaaagaata actactgtgt tactcctggt tggaactcga
     5281 tgcgattcgc gaagcctctg cttccaggag ccaagtatcg gtcgtatgtc aagatgattc
     5341 ctacagtcga ggatccgact atttacaatg gcgacgtgta catcatgcaa gatgatacca
     5401 tcatgggtgt ggttggtagc atacagttcc gcagatatcc gcgcatcctt cttagccgct
     5461 ttttctcgcc gccagacaag atggccacct tcgatgtcaa gccaaagggt caggtggctg
     5521 cccccaaaac tgcggtccca gcacaagatg caacaaaatt tgatgcaaaa gtgtcactcc
     5581 cgatacttgc tcgcggtgca tccgaacctg acagtgacga caagcagggc aaccctcctg
     5641 cccctgcact gcctgccccc gatgccgccg ccccatctat cgttgaatcc gcggccgctt
     5701 ccggcactac aactgcaaag gcaatcatgc tcattgccaa cgaggcaggc ctagaacttt
     5761 ctgagatgtc agacgacgcc cttttcgtta atttgggaat tgacagcttg atgtctctag
     5821 ttatcgcaga aaagctcaga tcagagctag aagtcaaggt cagcggcagc ctcttcttgg
     5881 actacgagac tattggtgat ttacggcagt ggttggatga gtattatagc tagggagatt
     5941 gataagactt ttctagttgc atatctttta tatttaaatc ttatctatta gttaattttt
     6001 tgtaatttat ccttatatat agtctggtta ttctaaaata tcatttcagt atctaaaaat
     6061 tcccctcttt tttcagttat atcttaacag gcgacagtcc aaatgttgat ttatcccagt
     6121 ccgattcatc agggttgtga agcattttgt caatggtcga aatcacatca gtaatagtgc
     6181 ctcttacttg cctcatagaa tttctttctc ttaacgtcac cgtttggtct tttatagttt
     6241 cgaaatctat ggtgatacca aatggtgttc ccaattcatc gttacgggcg tattttttac
     6301 caattgaagt attggaatcg tcaattttaa agtatatctc tcttttacgt aaagcctgcg
     6361 agatcctctt aagtatagcg gggaagccat cgttattcga tattgtcgta acaaatactt
     6421 tgatcggcgc tatgcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     6481 ggttaattgc gcgcttggcg agactcaaac aagatatatt ttgcccgcct tacagacgaa
     6541 actagttgga ggttatggag catactatca cgtgggcggc cactggtgag ttactacacc
     6601 ccaggggcaa cgttgatgct cctaaaaaac tctggctgga cgcaagccgt aacacccgtg
     6661 tcacttcata atcgtttgca attcagggct tgatctacac attaccctgt tatccctatt
     6721 aattaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7261 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7441 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7501 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7561 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7621 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7681 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7741 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7801 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7861 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7921 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7981 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8041 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8101 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8161 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8221 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8281 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8341 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8401 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8461 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8521 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac
     8581 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     8641 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     8701 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
     8761 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     8821 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     8881 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     8941 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     9001 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     9061 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     9121 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     9181 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     9241 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     9301 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     9361 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     9421 ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg
     9481 tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct
     9541 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
     9601 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
     9661 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
     9721 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
     9781 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
     9841 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
     9901 atttcctttg atattggatc atactaagaa accattatta tcatgacatt aacctataaa
     9961 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    10021 ggcgcgccat taccctgtta tccctaatat acaacgcaaa gaagctggaa aattggcgag
    10081 ggaatcctgt ttctgtctat ccaagaatgg gcatgaggtg gcaaccgtcg tgctagcgta
    10141 cagggtgcac tttgtaacca tttgggacac cggacactcg ctgttttcga aattaccctt
    10201 taagcgcggg tattgaacca ggcttatgcc caagatcgta gcaagcattt aactgtggga
    10261 atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct
    10321 caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat
    10381 tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag
    10441 aaaaaggaaa ggtgagaggc cggaaccggc ttttcatata gaatagagaa gcgttcatga
    10501 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt
    10561 tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc
    10621 aatatatata tatatttcaa ggatatacca ttctaatgtc tgcccctatg tctgccccta
    10681 agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta
    10741 aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt
    10801 taattggtgg tgctgctatc gatgctacag gtgtcccact tccagatgag gcgctggaag
    10861 cctccaagaa ggttgatgcc gttttgttag gtgctgtggc tggtcctaaa tggggtaccg
    10921 gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca
    10981 acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac
    11041 aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg
    11101 gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc
    11161 cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat
    11221 tgcctatttg gtccttggat aaagctaatc ttttggcctc ttcaagatta tggagaaaaa
    11281 ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg
    11341 attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca
    11401 gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt
    11461 tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg
    11521 aaccatgcca cggttctgct ccagatttgc caaagaataa ggttgaccct atcgccacta
    11581 tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca
    11641 ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt
    11701 ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt
    11761 aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa attcataata
    11821 gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca
    11881 atctacatac atttatcaag aaggagaaaa aggaggatag taaaggaata caggtaagca
    11941 aattgatact aatggctcaa cgaaatgacg gtatcgataa gcttgatatc gaattcctgc
    12001 agcccggggg atccactagt tctagagtta attaa
//
