LOCUS       sequence-217875-       13113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU35.2-2.2
FEATURES             Location/Qualifiers
     source          1..13113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1503..1802
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3808..4028
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4029..4832
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9260..9280)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9260..9278)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9617..10205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9697..9716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10376..11167,11168..11236))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10999..11018
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11237..11341)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11368..12710
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12331..12378)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12751..12769
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13088..13104)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13088..13104)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggagaacg acaagggcgg atataggcag atcaataagt
      541 cgttgaacat ctgtgccttt gatgagtatc ttaaaggtca gaccgcacga ctgccgcata
      601 tcgagagtat tgaacagctt actccaagag tgcttcgcgt tcttggccag aaccctggaa
      661 agtttacgca tcaagggaca aacacataca tcgtcggcac tggccaacga cgcctcatca
      721 ttgataccgg tgggggcgag ccagcatggg ccaaactgat tgactcaaca tttatatcaa
      781 tgaacatcag cctctcatat gttctgctga cccattggca cggtgaccat accggcggag
      841 ttccagctct tctgcgccta tacccacatc tcacgaattc catctacaag aacgaaccgg
      901 accaaagcca acagaacatc acccacggac agatattttc tgttgacggc gcaacaatcc
      961 gtgctattca tgtgcctgga cattcagaag atcacatgtg ttttatctta gaagaggagc
     1021 aggcaatgtt taccggtgac aatatccttg gccacggtac cagtgctgta gaagacctcg
     1081 gcacgttcat ggccagccta cagaagatgg ctgcccagga ttgccatatc ggttattctg
     1141 ctcatggcgc cacagtcacc gacttgccag ccaaaattac cggggaatta gataacaagt
     1201 tgcggcgcga gaagcaagtt atgaatgcgc ttggcagagt ccgtagccgc ggcgagaaga
     1261 gcgtcaccac caaagacttg gtaacagaga tctacggcga gtcgcttgac gaagatacga
     1321 ggatactcgc attggagcca tttatcgacg aagtgctacg gaaactagcg aatgactgta
     1381 aggttgcatt cgaaatgagg agaggcaaga agaagtggtt ctctgtggag gaaatggagg
     1441 agacaaccac gcagcggttt atggctctga aaaatccact ggccgtaact attcaagttt
     1501 aagcggatct cttatgtctt tacgatttat agttttcatt atcaagtatg cctatattag
     1561 tatatagcat ctttagatga cagtgttcga agtttcacga ataaaagata atattctact
     1621 ttttgctccc accgcgtttg ctagcacgag tgaacaccat ccctcgcctg tgagttgtac
     1681 ccattcctct aaactgtaga catggtagct tcagcagtgt tcgttatgta cggcatcctc
     1741 caacaaacag tcggttatag tttgtcctgc tcctctgaat cgtctccctc gatatttctc
     1801 attttccttc gcatgccagc attgaaatga tcgaagttca atgatgaaac ggtaattctt
     1861 ctgtcattta ctcatctcat ctcatcaagt tatataattc tatacggatg taatttttca
     1921 cttttcgtct tgacgtccac cctataattt caattattga accctcacga tccagttctc
     1981 cagtgacaca gcctttatct ggtcaaacct ttctttctaa tcacctatgc tgatgcttaa
     2041 ttaagggatt tttgtctcca tcaacggcat gcgcccaaaa atgacgtttt ttttaaccca
     2101 tagacacgaa actacccatt ttccaccggc ctgacctacc accggaacaa cggccatctc
     2161 caacttgcaa gttggggaaa ttaagagcat cgcaggttta atggaagaaa aaaaaaaggt
     2221 acagcacagc gcaaatggag ttagttccct tatgtcacac actcacacac agtcggtcag
     2281 atcaagcata ctgggtgcgt ataaatagag tggccattgc caccctgttt atctcaaaat
     2341 ctgtcttgtt agtggtcttc tccctttttc aggttacaat tctcttgttt ctacttagta
     2401 tataagtata tcaagctata ttaagcatac tatcaactgt caactctatc ctcaaaatac
     2461 aatacaaaat gactttccat cccgacactc tacccgtcct caagggcaaa gtattcatag
     2521 tcaccggtgg gaactccggc ataggctact ataccgtatc ccacctagca gaacatggcg
     2581 cccacgtcta catgtgcgcc cgatccccaa agaagggcac agaagccatc agaaacatca
     2641 agaaaatgca cccctcagcc aatatcgacc tcctgcaaat ggacctcatg gacctcacca
     2701 gcgtcgtcgc agccgccaag ttattcatca ccctcgaaac aaccctgcat ggcctggtca
     2761 acaatgcagg catcatggct acgccttttg aaatgacgaa ggatggttac gaggcccagt
     2821 ggcagaccaa ctacctctcc cactgggttt tgacggagca tcttctccct ctcatgttgc
     2881 agacttccaa gacgctttct cctggcagcg tccgcatcgt caatctaact tcatcgggtc
     2941 acttgagcgc acccaagggc ggtatcaatt tcgatgatct atcactcaag gacagcggcc
     3001 catggtcacg atacggacag agcaagctgg ccaatattct gcacaccaag actctgcaaa
     3061 attcgtacgg ccccggttct cccagtgcgc agaacggcgg gggcgagatc tgggtttcgt
     3121 ctgtgcatcc cggcgtggtt gatacgaacc ttgccacatc ggtggaggag tccgggacag
     3181 gcatttcgag tcttctctca atcatgcgca tggtcggatt gatatggcct gcggataagg
     3241 ggtcgtggaa tagtgtgttt tgtgcggcta gtcaggatat gacggcggag cagagcgggg
     3301 cgtatatgga gatctttaag cgttttggag agctgaggtg ccagagtggt gcggcgaagg
     3361 atgagcagct tgcggagagg ttagaggagt ggactagggt ggcgatggct gtggtttcta
     3421 agtgaacaaa tcgctcttaa atatatacct aaagaacatt aaagctatat tataagcaaa
     3481 gatacgtaaa ttttgcttat attattatac acatatcata tttctatatt tttaagattt
     3541 ggttatataa tgtacgtaat gcaaaggaaa taaattttat acattattga acagcgtcca
     3601 agtaactaca ttatgtgcac taatagttta gcgtcgtgaa gactttattg tgtcgcgaaa
     3661 agtaaaaatt ttaaaaatta gagcaccttg aacttgcgaa aaaggttctc atcaactgtt
     3721 taaaaggagg atatcaggtc ctatttctga caaacaatat acaaatttag tttcaaagat
     3781 gaatcagtgc gcgaaggaca taactcattc aattcaattc atcatttttt ttttattctt
     3841 ttttttgatt tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag
     3901 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
     3961 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa
     4021 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
     4081 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
     4141 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
     4201 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
     4261 aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg
     4321 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
     4381 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
     4441 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
     4501 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
     4561 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
     4621 tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata
     4681 gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat
     4741 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
     4801 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
     4861 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc tatgcggtgt
     4921 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggataggaaa aaaccgagct
     4981 tcctttcatc cggcgcggct gtgttctaca tatcactgaa gctccgggta ttttaagtta
     5041 tacaagggaa agatgccggc tagactagca agttttaggc tgcttaacat tatggatagg
     5101 cggataaagg gcccaaacag gattgtaaag cttagacgct tctggttgga caatggtacg
     5161 tttgtgtatt aagtaaggct tggctgggga tagcaacatt gggcagagta tagaagacca
     5221 caaaaaaaag gtatataagg gcagagaagt ctttgtaatg tgtgtaactt ctcttccatg
     5281 tgtaatcagt atttctactt acttcttaaa tatacagaag taagacagat aaccaacagc
     5341 ctttcccaga tatacatata tatctttatt tcagcttaaa caataattat atttgtttaa
     5401 ctcaaaaata aaaaaaaaaa accaaactca cgcaactaat tattccataa taaaataaca
     5461 acatggatag attgacggaa ctcaatggtc atagcgatca gctcgcccaa gctatcagga
     5521 gcctcgtcga tagccgtcga ggagccgatt cttctgacca gctggggcca agtgctgaca
     5581 ctgatgtaga cagagatcaa tctttattca caagagccaa gtcaaatatt ctggccagta
     5641 ttgcagcaat caagtccata gttggtgaac cagcagactt cctccaggat ttttctcgcc
     5701 agatcgaaat ccttgcttgt cttcggtggc tagcggaatt ccaagttttt gcgtgcatcc
     5761 cgtttgacga aagcttgcct atcaaggatt tggcggacct agtaggagtt cccgagagcc
     5821 agttggttcg tgtcatccgt ctgactgcca catgcggatt tctgcgggaa ccgatactag
     5881 gctttgtgtc ccacagcccc ctctcggcgc aattcatatc caaccagtca ttagttgacg
     5941 ctatggtgtt tattgccgag ttggctactc ccacagcatt gcagatgccg caagcaacgc
     6001 agcgatttgg agcctcgtcg agtgccaccg agagcgcata caacttagct ctcaatacgg
     6061 tgcggccgtt tcatgtggct atccaagaac gctctaagct gcgtcgtcaa tggtcagctt
     6121 acctttgcca tgcagcaggg ttgcaccagg aggaagaaat catcgaagtg ttatcgcggc
     6181 tcaactggtc taaccttggt aatgcgtgca tagtcgaggt aggggcaaag tcgacctcga
     6241 tggcaagctc tctttccaaa atgtttccaa gcttgcggtt ggtcgtgcaa atcgacaata
     6301 cacaatcttc tttgctagat cgagactaca tgtggcaaaa tgcagttctg gcaagctcaa
     6361 tccgggacag ctctagctct ggatctagcc cttctcccac aaaccctttt atcacggtta
     6421 cctatcatgc catgggcatg ccgcagtctg tgacagatgc tgccgtttat attattcacg
     6481 tgcctgccac atcctcagga ttgacgatta aagcagaact ggatgattac ttaggtgtgc
     6541 ttcgtaccag cgggggcatc atgcttatct tgacgacacg actgcttccc gagccaggca
     6601 ctcttcccaa cccagagatc gaggccgtgg cgcgcgcacg ggatctgagc atgctccaac
     6661 tggccaacga gagcgagatg gagatgtccg agcttctgag tataataaag acggtggggg
     6721 acaacgctgg gaaactcgtt gtcactaatc accttcgctc aaacaatggc gtcatcctag
     6781 ctttaactat caagtaccaa gcatattaaa gtgcttttaa ctaagaatta ttagtctttt
     6841 ctgcttattt tttcatcata gtttagaaca ctttatatta acgaatagtt tatgaatcta
     6901 tttaggttta aaaattgata cagttttata agttactttt tcaaagactc gtgctgtcta
     6961 ttgcataatg cactggaagg ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt
     7021 gcagtttgaa aaataactac atggatgata agaaaacatg gagtacagtc actttgagaa
     7081 ccttcaatca gctggtaacg tcttcgttaa ttggatactc aaaaaagatg gatagcatga
     7141 atcacaagat ggaaggaaat gcgggccacg accacagtga tatgcatatg ggagatggag
     7201 atgatacctc cattgggccg atgaagttag tcgacggata gaagcggttg tcccctttcc
     7261 cggcgagccg gcagtcgggc cgaggttcgg ataaattttg tattgtgttt tgattctgtc
     7321 atgagtatta cttatgttct ctttaggtaa ccccaggtta atcaatcaca gtttcatacc
     7381 ggctagtatt caaattatga cttttcttct gcagtgtcag ccttacgacg attatctatg
     7441 agctttgaat atagtttgcc gtgattcgta tctttaattg gataataaaa tgcgaaggat
     7501 cgatgaccct tattattatt tttctacact ggctaccgat ttaactcatc ttcttgaaag
     7561 tatataagta acagtaaaat ataccgtact tctgctaatg ttatttgtcc cttatttttc
     7621 ttttcttgtc ttatgctata gtacctaaga ataacgacta ttgttttgaa ctaaacaaag
     7681 tagtaaaagc acataaaaga attaagaaaa tggacgcctt cagagactct actcctcagg
     7741 aatctgccta ttccgcccct caggttgccg cctggctcgg tcgcatctcc ctcccctcat
     7801 ccctaaccca atacgtggat tctccgtcag cctttcctaa gacagccaca tccctccagt
     7861 cgctgttccg atgccagatc acaacctttc cttacgagaa tctctcagtg cactactccc
     7921 agagccacca ggtcaacatc cagccagatg tgctgtttac gaaaatgatg ggcccggatc
     7981 ataacggcag gggcggctac tgcatggaac ttagcatctt cttccaccac atgctgcgcg
     8041 ggcttgggtt ccacgtctac atgacgggcg tgcgcaaccg gacgcggact gacggcaagc
     8101 cgcagggcga atatcagggg tggacgcaca ttaacaacat cgtccacctc ccttgcggct
     8161 ccaagtttag cgccgacgtc gcgtttggtg gtgacgggcc gacgtctcct cttcctatgg
     8221 atggcatggc atctgcgctt cgcaatctag gaacccaaga ggtacgcctc atacaggaaa
     8281 acattcctaa gcagcgcctt cgggagccga agttgtgggt gtaccagtat cgcaacagtt
     8341 ctgataagga gtggaactca ttctacagct tttctgagat cgagttcttt caggaggatt
     8401 ttgaggttca gaattggtgg actagcgcta agacactcca tcggtggact gtgttagtgg
     8461 ttcgatttct aagggagggt gagcccatta aattctctag agatggtgcc tggaggaaag
     8521 gagatgacca agagataagc attgtcggca aggtaatgct agtaaataac ctggttaagg
     8581 ttaatgtagg ggggaagaca agggttgtac atgagtttaa ttccgaggat ggacggttgc
     8641 tggcactcca ggagtatttt gggattacat taacgcagga ggaggcccag ttcatacagg
     8701 ggtgggacat ggcgctaaaa tagggagatt gataagactt ttctagttgc atatctttta
     8761 tatttaaatc ttatctatta gttaattttt tgtaatttat ccttatatat agtctggtta
     8821 ttctaaaata tcatttcagt atctaaaaat tcccctcttt tttcagttat atcttaacag
     8881 gcgacagtcc aaatgttgat ttatcccagt ccgattcatc agggttgtga agcattttgt
     8941 caatggtcga aatcacatca gtaatagtgc ctcttacttg cctcatagaa tttctttctc
     9001 ttaacgtcac cgtttggtct tttatagttt cgaaatctat ggtgatacca aatggtgttc
     9061 ccaattcatc gttacgggcg tattttttac caattgaagt attggaatcg tcaattttaa
     9121 agtatatctc tcttttacgt aaagcctgcg agatcctctt aagtatagcg gggaagccat
     9181 cgttattcga tattgtcgta acaaatactt tgatcggcgc tatgcggccg ccaccgcggt
     9241 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg agactcaaac
     9301 aagatatatt ttgcccgcct tacagacgaa actagttgga ggttatggag catactatca
     9361 cgtgggcggc cactggtgag ttactacacc ccaggggcaa cgttgatgct cctaaaaaac
     9421 tctggctgga cgcaagccgt aacacccgtg tcacttcata atcgtttgca attcagggct
     9481 tgatctacac attaccctgt tatccctatt aattaatacg gttatccaca gaatcagggg
     9541 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9601 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9661 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9721 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9781 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9841 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9901 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9961 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10021 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    10081 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10141 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10201 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10261 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10321 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10381 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10441 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10501 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10561 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10621 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10681 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10741 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10801 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10861 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10921 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10981 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11041 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11101 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11161 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11221 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11281 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11341 gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa
    11401 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
    11461 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
    11521 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
    11581 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
    11641 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
    11701 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
    11761 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
    11821 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
    11881 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
    11941 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
    12001 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
    12061 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
    12121 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
    12181 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
    12241 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
    12301 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa
    12361 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
    12421 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
    12481 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
    12541 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
    12601 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
    12661 attggattag tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa
    12721 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    12781 gcgcgtttcg gtgatgacgg tgaaaacctc ggcgcgccat taccctgtta tccctaatat
    12841 acaacgcaaa gaagctggaa aattggcgag ggaatcctgt ttctgtctat ccaagaatgg
    12901 gcatgaggtg gcaaccgtcg tgctagcgta cagggtgcac tttgtaacca tttgggacac
    12961 cggacactcg ctgttttcga aattaccctt taagcgcggg tattgaacca ggcttatgcc
    13021 caagatcgta gcaagcattt aaatgacggt atcgataagc ttgatatcga attcctgcag
    13081 cccgggggat ccactagttc tagagttaat taa
//
