LOCUS       sequence-217877-       15711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15711)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 15711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU37.2-2.2
FEATURES             Location/Qualifiers
     source          1..15711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2598..2897
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        5863..6083
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             6084..6887
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(11858..11878)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11858..11876)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(12215..12803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12295..12314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12974..13765,13766..13834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13597..13616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13835..13939)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13966..15308
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(14929..14976)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     15349..15367
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15686..15702)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15686..15702)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggttgccc ctcctccaga ctgggtcgcg aagttgacgc
      541 cctccgggcc gcagggcagt gagctccttg caacggagcg tgcgaagagt aacatcaacg
      601 ttgagaagct gtccaacttc atgttcggca aggaggcgct ggagaacgag gccaagatcc
      661 tggctatcct ccagtccgag aaggtgttcg acaagtcgaa caatgccttc ctgggaagag
      721 tggagaagtt cccggttgct ctcgccagag cgaagaagct gagacagctg caagtcaagc
      781 acaactggag caccgaggac tacagaatcg ccgatggcct gatctccgag ccaggcccat
      841 acgggcttca cgcctctatg ttcttgactg ctctcaggga ccaaggaact tcggaacaac
      901 acaagctctt ccttgagccg gccgagaact acaagtacat cggctgctac gcccagaccg
      961 agttgggaca cggctccaac gttcgtggtc ttgaaaccac cgcgacctgg gaccctaccg
     1021 acaagacctt cgtcattcac tctcctcatc ttaccgcctc caagtggtgg attggctctt
     1081 tgggccgcac cgccaactac gccgtcgtta tggcacagct tattctcaac ggcaagtcct
     1141 acggaccaca ccccttcgtt gtcccagtca gggacttgaa gactcaccag ccccttgacg
     1201 gcgtctacgt cggagacatt ggaccaaaat tcggttacaa caccatggat aacggtttcc
     1261 tcctcttcaa caaggtcaag gtaccccacg tcaacatgtt ggcaaagtac tccagcgtcg
     1321 acccaaacac aaacaagtat gtccgcccag cagctgcgtc gttggtttac ggaactatga
     1381 cctgggttcg ctccaccatt gtgcttcaat ccggccagac cctggcaaga ggtgtcacta
     1441 tcgccaccag gtattgtgct gttcgcaggc aattccagga ccgcgatgac atctccaacg
     1501 atgtcaccga gaaccaagtt ctcaactaca gcatggtgca attccgtctt ctccctctcc
     1561 tcgcttccac cttcgctctc cacttcactg gcaaggccat gatggaaatg tacgcacaaa
     1621 accaagagag gatgaagaag gctgttgcca acaccaggag aggagccggc ccagaggagc
     1681 ttcagtctgg cagtgatatg cttgctgatc tccacgccac ttcttgtgga cttaaggcgc
     1741 tcggcagcac catcgctgct gagggtctgg aggtgtgcag aagagcttgc ggtggccacg
     1801 gttactccca gttctctggt atcggcagct tctacgccga ttacctccca accaccacct
     1861 gggagggaga caactacatg ttgacccagc aagtcgcacg ctacctcctc aagattgccc
     1921 gctccgtcct caagggcaag gcacccgtca acgacaccgc cgagatcttc cgcacctact
     1981 tggacgcaca ggacaagggc tgcgccttcg acatcctctc caacgacggc gacatcgtcg
     2041 cagccttcgc atggcgcaca tcctacctca ccttcgaggc cctcaagcac cgcgacggcg
     2101 agaagaagtc gtggaactcc ctcctcgtcg acttctggcg cctgtccact gcccactcgc
     2161 agtacctcgt cgtcaagaac ttctacgacg ccctgcagtc ctcgtcgctc acctacgagc
     2221 tcgactccgt ctccatcacc accatgcacc agctcttccg cctcttcgcc ctcagcaccc
     2281 tcgagaagga ggccgccgag ttctactcct cctccgccct cacagtccgc cagatccagc
     2341 tcgtccgcac aaagtcggtc atgcagctcc tcgccgacat caggccgcac gccctgcgcc
     2401 tggttgacgc ttggcagttc ccggattggc agctcgacag cagtctgggc cgcaaggatg
     2461 gtaaggtcta cgaggatatg ttccacaggg ccagccagct gaacccgctg aacggtctga
     2521 ccgtggaccc atacccagag agtgatgtgt tgatcaagag cgatgagacg aaccgtgctt
     2581 tgcaggcgaa gttgtaggcg gatctcttat gtctttacga tttatagttt tcattatcaa
     2641 gtatgcctat attagtatat agcatcttta gatgacagtg ttcgaagttt cacgaataaa
     2701 agataatatt ctactttttg ctcccaccgc gtttgctagc acgagtgaac accatccctc
     2761 gcctgtgagt tgtacccatt cctctaaact gtagacatgg tagcttcagc agtgttcgtt
     2821 atgtacggca tcctccaaca aacagtcggt tatagtttgt cctgctcctc tgaatcgtct
     2881 ccctcgatat ttctcatttt ccttcgcatg ccagcattga aatgatcgaa gttcaatgat
     2941 gaaacggtaa ttcttctgtc atttactcat ctcatctcat caagttatat aattctatac
     3001 ggatgtaatt tttcactttt cgtcttgacg tccaccctat aatttcaatt attgaaccct
     3061 cacgatccag ttctccagtg acacagcctt tatctggtca aacctttctt tctaatcacc
     3121 tatgctgatg cttaattaag ggatttttgt ctccatcaac ggcatgcgcc caaaaatgac
     3181 gtttttttta acccatagac acgaaactac ccattttcca ccggcctgac ctaccaccgg
     3241 aacaacggcc atctccaact tgcaagttgg ggaaattaag agcatcgcag gtttaatgga
     3301 agaaaaaaaa aaggtacagc acagcgcaaa tggagttagt tcccttatgt cacacactca
     3361 cacacagtcg gtcagatcaa gcatactggg tgcgtataaa tagagtggcc attgccaccc
     3421 tgtttatctc aaaatctgtc ttgttagtgg tcttctccct ttttcaggtt acaattctct
     3481 tgtttctact tagtatataa gtatatcaag ctatattaag catactatca actgtcaact
     3541 ctatcctcaa aatacaatac aaaatgtccg aacctcagag caatccctct atcgagttga
     3601 agagcgtggc cccagacact ttctcatcgg attcggagag ggggtttgag cacaatgcgc
     3661 aattgaaaaa cactaccgtc aagcatttca tttggcaaga tgtcacggtc acagtgcagg
     3721 accgggccac aaagcagtcc aaggcaatct tagagaatct gaatggagtt gtctcagctg
     3781 gcgagatgtg tgctttgatg ggcccgagtg gttctggaaa gacaactctg ttgaacttcc
     3841 tcgccagacg cgacaaccct ggagctgatg ccaccggctt tgcatctgta aatggaaacg
     3901 ccatatccct caatgagttc aggaaaatga gctcctacgt ggaacaagag gatgccttga
     3961 ttgggtcttt gacggtcaga gagactatga actttgctgc acgcttgtca aactctggcc
     4021 tcacaaagaa ggatcgtatc atgagaatcg atggcttgat tgattcgttt ggtcttcgga
     4081 aacaggaaca cactatcatt ggcacgccaa ttcgtcaggg gattagcggt gggcagaaac
     4141 gccgtgtcag tgtagcaagc cagttgatta cggcgccaaa gatcttattc ctggatgaac
     4201 caacaagcgg cctcgactct gcggctagct tcgaagttgt ttctttcatt aagaccttgg
     4261 ccaagcaaaa caatttgatc gtaattgcta gcatacacca gccgtccacg cagacgttct
     4321 cactattcga taagctgctc ctcctgtcag agggtaaacc gcactatttt ggagctgtgg
     4381 acgatgtggt tccatactat ctggagatcg actacccggt gccattgcat atgaaccccg
     4441 ccgaacatgc attagaattg accaacaagg attttggatc gggcctggtc gaggcagaag
     4501 aagatttatc caagctccag tcagcctggt cttctaatcc acgtgccaaa gcgattgcca
     4561 gccagatcaa ctcctatctc atcactgcca gcgagggcca aacacataat tcgaagaccg
     4621 gctctcatgc aaactttgcc gcggtcacat tgaccctcgt tcatcgctct tttatcaagt
     4681 cgtaccgcga tgtcgtggca tacggaattc gcctagcaat gtacagctgt cttgctatca
     4741 tgagcgggac ggtttggcta cgcctaagcc cttcccagac gtccattcaa cctttcatca
     4801 acgccatatt tttcggagct tcgtttttat cgttcatggc tgtcgcatac gtgccgtcat
     4861 tcctcgaaga caggttaact tttatcaaag agcgctcaaa cggcctctat ggtgccgctg
     4921 ccttcacagt tgccaatttc atcattggac tcccatatct gtttggcatt gcactcatat
     4981 ttacgtgcat ctcgtactgg ctgaccggct tccataatac cgcccctgct ttcttcacat
     5041 tcgtcatgtg ggtattcctt gacatggtag ccgcagagag cttggtagtc ctaatgtcct
     5101 cgatctttcc caacttcgtc gtcgccctcg ctctcacagc tttcgtgaac ggtctgtgga
     5161 tgtgcgttgg cggcttcctg atttccacgc ctctcttgaa cgtgttctgg aaatacgtgt
     5221 tccactacat cgattaccaa gcctatgtct tccaaggatt gatggtcaac gagtttgccg
     5281 accgcacgta caactgtgac caggactgtc actgcatgta caccacggaa ctggcctcac
     5341 aatgcatgat ctctggaaag ggagtcttgg acacgtttgg gatgtccacc ggcctgacag
     5401 gcacgtgggt tgggatctta attggaattg tagcagtgta tagacttctt ggatgggctg
     5461 tgttggcact caagcgttaa acaaatcgct cttaaatata tacctaaaga acattaaagc
     5521 tatattataa gcaaagatac gtaaattttg cttatattat tatacacata tcatatttct
     5581 atatttttaa gatttggtta tataatgtac gtaatgcaaa ggaaataaat tttatacatt
     5641 attgaacagc gtccaagtaa ctacattatg tgcactaata gtttagcgtc gtgaagactt
     5701 tattgtgtcg cgaaaagtaa aaattttaaa aattagagca ccttgaactt gcgaaaaagg
     5761 ttctcatcaa ctgtttaaaa ggaggatatc aggtcctatt tctgacaaac aatatacaaa
     5821 tttagtttca aagatgaatc agtgcgcgaa ggacataact cattcaattc aattcatcat
     5881 ttttttttta ttcttttttt tgatttcggt ttctttgaaa tttttttgat tcggtaatct
     5941 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
     6001 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
     6061 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
     6121 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
     6181 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
     6241 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
     6301 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
     6361 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
     6421 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
     6481 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat
     6541 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga
     6601 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
     6661 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
     6721 tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg
     6781 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa
     6841 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
     6901 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
     6961 taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggata
     7021 ggaaaaaacc gagcttcctt tcatccggcg cggctgtgtt ctacatatca ctgaagctcc
     7081 gggtatttta agttatacaa gggaaagatg ccggctagac tagcaagttt taggctgctt
     7141 aacattatgg ataggcggat aaagggccca aacaggattg taaagcttag acgcttctgg
     7201 ttggacaatg gtacgtttgt gtattaagta aggcttggct ggggatagca acattgggca
     7261 gagtatagaa gaccacaaaa aaaaggtata taagggcaga gaagtctttg taatgtgtgt
     7321 aacttctctt ccatgtgtaa tcagtatttc tacttacttc ttaaatatac agaagtaaga
     7381 cagataacca acagcctttc ccagatatac atatatatct ttatttcagc ttaaacaata
     7441 attatatttg tttaactcaa aaataaaaaa aaaaaaccaa actcacgcaa ctaattattc
     7501 cataataaaa taacaacatg accgaaccat cgcccccgga gaaaagttcg ccacagtcgc
     7561 cttctactgc tgcgcatgtt actccaaatg cttcaagtga aggcgcgatt gtcgacttcg
     7621 atggcgctgg tgatccagcg aacccgttga actggtcgtt ttcaagaaaa gtggttacga
     7681 gtggtcttta cagtctgaca agccttggat ctgtgtgggc aagcacagca tactcccctg
     7741 ccaatagcaa ggtcgccgat gagtttcatg tgtcaccaga agtagctatc atcgggacga
     7801 gccttttact cttgggctgg ggatttggac cgttactctg ggcgccgctg tcagaagttt
     7861 acggccggaa atggccagtt ttgatcccat tcttcatctc tattgtgatg tcatttggga
     7921 cggcgacggc aaaggatatc caaacgattc tgattactcg gttcttcacc ggattctttg
     7981 gggctgctcc tatcacatgc acaggaggag tctatgttga catctgggat gcatcccaac
     8041 ggggaaatgc tattgttggg tacacgctcg ccgtgtgtgg aggtccggct cttggacccg
     8101 tcattggagg cgctattatt tacagcggca caaattggag atggactgaa tatactacag
     8161 gcatattgca agcctcgata cttattccag gagtaatatt catcgaagaa tcatacgtac
     8221 caatgttact cgtcagaaag gcacgcaagc tgagacagaa cacgggaaac tgggcactgc
     8281 acgccgagtg ggaagaaacc ggtgtttcca tcaaggcact cgccataaag ttcggcctac
     8341 gacctcttca gatgctattg acgccaattt gcctctcggt taccatctac tcatcattta
     8401 tatacggcac attttatgcc agcctggcat cgttccccat catcttccaa gaaacgcgag
     8461 gctggaacga gctcgtcggg tcgcttccgt tcctcggagt gcttatagga atcattatgg
     8521 gcgcagcact cagcgctctg aaccagaaat actacaacat cgcgtacgaa gctaacgact
     8581 tcaaaccggt tccggagtct cgcctcccgc caatgatgat tgcttcagtc gttttatcgg
     8641 gcggattgtt catcatggga tggacttctt cccccgacct cccgtggata gcaactttaa
     8701 taggagtcac tatgatgggg tttggatact atacgatttt taccagcgcc ttgaattatc
     8761 tcgtcgatac attccagcgg tggggtgcca gtgctatagc cgcaaatacg ttcatgcgaa
     8821 gtgtgctcgc ggctgcattc ccgcttattg tcccgccaat gttccgacgt ctcggaaatg
     8881 aatgggcatt aagtgtgctc gccatcttct cggtgtttaa tatcccgatt ccgttcgtct
     8941 tttggtttta tgggcctcgg atcagagcga tgggcaaata cagctcgaat atggggtaga
     9001 gtgcttttaa ctaagaatta ttagtctttt ctgcttattt tttcatcata gtttagaaca
     9061 ctttatatta acgaatagtt tatgaatcta tttaggttta aaaattgata cagttttata
     9121 agttactttt tcaaagactc gtgctgtcta ttgcataatg cactggaagg ggaaaaaaaa
     9181 ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa aaataactac atggatgata
     9241 agaaaacatg gagtacagtc actttgagaa ccttcaatca gctggtaacg tcttcgttaa
     9301 ttggatactc aaaaaagatg gatagcatga atcacaagat ggaaggaaat gcgggccacg
     9361 accacagtga tatgcatatg ggagatggag atgatacctc cattgggccg atgaagttag
     9421 tcgacggata gaagcggttg tcccctttcc cggcgagccg gcagtcgggc cgaggttcgg
     9481 ataaattttg tattgtgttt tgattctgtc atgagtatta cttatgttct ctttaggtaa
     9541 ccccaggtta atcaatcaca gtttcatacc ggctagtatt caaattatga cttttcttct
     9601 gcagtgtcag ccttacgacg attatctatg agctttgaat atagtttgcc gtgattcgta
     9661 tctttaattg gataataaaa tgcgaaggat cgatgaccct tattattatt tttctacact
     9721 ggctaccgat ttaactcatc ttcttgaaag tatataagta acagtaaaat ataccgtact
     9781 tctgctaatg ttatttgtcc cttatttttc ttttcttgtc ttatgctata gtacctaaga
     9841 ataacgacta ttgttttgaa ctaaacaaag tagtaaaagc acataaaaga attaagaaaa
     9901 tgcggccccg gctcccattc gacgatgttg cttgggaaca aagtgacaac gtttacgaag
     9961 aatggcgaac taagcttctc aaaccagaaa atctaacgga aatcgctctt tttgttgcaa
    10021 agtatcacct tgggacgcca gtgaaggtta gaacgcctct tacgggcgcc tataatgtgg
    10081 cggtggttgt tgattttcgc ggaggtggtt cgacattgat tcgctttccc aaaccaggta
    10141 ggctgatgtt tcccgaggag aaggtcagga atgaagtcgc cgtaatgcgg tatattcaag
    10201 ctcatacgtc gatcccggtg ccgtttatat tgcactgggg cacgaaggaa cagagtcctt
    10261 gtggtctagg gccgtttatt attatggaat atgtcaatca tgactgtgat atcagtgacg
    10321 cgttgaatac gccgggaata ccatctgagg accgaccaat tcttgatcct gctgtagatg
    10381 aaggaagact ccggaaatta tatgggcaaa tggcagatgt tctgcttcaa ctatccatgt
    10441 tgtcattccc taaaataggc tctctcactc aagacgacga tgaagatact ttgactgtgg
    10501 ccgcaagacc attgacggca aatatgaacg aggtcgtaca gctcgggaca ttgccacgtt
    10561 caaagctacc tgataaaagt gcagcctttg aggcttcttc gtcatacctg actacactag
    10621 ccaatatgca ccttcgccac ttaacctacc agcgaaacga cgcgattgtc agcgcgagcg
    10681 actgctgcca aaaatatatc gcaagatacc tcttccgaaa gctttccgtt gaaggcaagc
    10741 tgtcacgtcc tgagcacaat ttcgggcctt ttaaactctg gtgcgatgat ctgcgcccag
    10801 ctaatatgct aattaattcc aacctcgagt tggtttccat tattgacagg gaatttgcat
    10861 atgctgctcc cgtggagttc tcacatgcgc ccccttggtg gctcctaata gaacagccag
    10921 agcactggtc ggaaggactt tacgcatggg ctactgctta tgaacctcgg cttcaaactt
    10981 tcttggccgt tcttcgcgag cgtgaagatg tcctcataga cgagggaaga cttaaagacc
    11041 aggagagact ctcgttgcct atgcgggaga gctgggacag tggtgatttc tgggtcgtat
    11101 atgctgctcg caagggcttt gcatttgatg cggtattctg gaagtatctg gatgcacgct
    11161 tctttggggc tgtggatgga gtggaagggg atgagtggga aaggagagcc gagttgctgg
    11221 acaaggagga acttatggaa attgacagct ttgtcgatca aaaagtggtt gaaatgaaaa
    11281 cccgagcctt agcatgggaa cccgaagaac atcttggata aggagattga taagactttt
    11341 ctagttgcat atcttttata tttaaatctt atctattagt taattttttg taatttatcc
    11401 ttatatatag tctggttatt ctaaaatatc atttcagtat ctaaaaattc ccctcttttt
    11461 tcagttatat cttaacaggc gacagtccaa atgttgattt atcccagtcc gattcatcag
    11521 ggttgtgaag cattttgtca atggtcgaaa tcacatcagt aatagtgcct cttacttgcc
    11581 tcatagaatt tctttctctt aacgtcaccg tttggtcttt tatagtttcg aaatctatgg
    11641 tgataccaaa tggtgttccc aattcatcgt tacgggcgta ttttttacca attgaagtat
    11701 tggaatcgtc aattttaaag tatatctctc ttttacgtaa agcctgcgag atcctcttaa
    11761 gtatagcggg gaagccatcg ttattcgata ttgtcgtaac aaatactttg atcggcgcta
    11821 tgcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
    11881 gcttggcgag actcaaacaa gatatatttt gcccgcctta cagacgaaac tagttggagg
    11941 ttatggagca tactatcacg tgggcggcca ctggtgagtt actacacccc aggggcaacg
    12001 ttgatgctcc taaaaaactc tggctggacg caagccgtaa cacccgtgtc acttcataat
    12061 cgtttgcaat tcagggcttg atctacacat taccctgtta tccctattaa ttaatacggt
    12121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    12181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    12241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    12301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    12361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    12421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    12481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    12541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    12601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    12661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    12721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    12781 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    12841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    12901 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    12961 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    13021 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    13081 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    13141 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    13201 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    13261 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    13321 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    13381 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    13441 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    13501 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    13561 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    13621 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    13681 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    13741 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    13801 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    13861 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    13921 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg
    13981 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
    14041 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
    14101 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
    14161 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
    14221 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
    14281 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
    14341 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
    14401 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
    14461 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
    14521 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
    14581 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
    14641 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
    14701 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
    14761 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
    14821 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
    14881 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
    14941 ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
    15001 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
    15061 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
    15121 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
    15181 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
    15241 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
    15301 attggatcat actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    15361 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctcgg cgcgccatta
    15421 ccctgttatc cctaatatac aacgcaaaga agctggaaaa ttggcgaggg aatcctgttt
    15481 ctgtctatcc aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca gggtgcactt
    15541 tgtaaccatt tgggacaccg gacactcgct gttttcgaaa ttacccttta agcgcgggta
    15601 ttgaaccagg cttatgccca agatcgtagc aagcatttaa atgacggtat cgataagctt
    15661 gatatcgaat tcctgcagcc cgggggatcc actagttcta gagttaatta a
//
