LOCUS       sequence-217878-       13010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU38.1-2.2
FEATURES             Location/Qualifiers
     source          1..13010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7445..7465)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7445..7463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7802..8390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7882..7901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8561..9352,9353..9421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9184..9203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9422..9526)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9553..10895
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10516..10563)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10936..10954
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11226..11630
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11643..12737
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12985..13001)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12985..13001)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgacaa
      541 aagctatgcg ggtcttcctc tttggagatc taaatgtctc cttcgaggag agcctcagag
      601 agttgctcta caccaaggac atgccagtct tgaaatcttt ccttgaaaag gtggcttttg
      661 cgcttcgtga ggagatttcc cgcatgtcct cttcctggca ggcgttaatg ccacgtttta
      721 ctaccttaat cgatctgctc cctcagatca atggcaagat aggaagtccg gcgcttagat
      781 ttgctttgct ctgtgtggcg gagctaggac tgttcattcg cttccatgag cgaaggtcta
      841 ttccatatcc taccggggac aactcgtgtc tcttgggtat ctgcactgga tcttttgctg
      901 cagctgcagt cgccgtatcc cgtggcctta cggacctcgt tcccgctggc gtcgaggcga
      961 ctcttgtagc cctacgtaca gccacactct cgctgcaatg tcgctttgac atgtgcaaca
     1021 tgacagagac aggctcatcc tgctggtccg ctattgtcaa cctcacggaa gaggacgcta
     1081 ctaagcttgt tgatgctttc tgtgaagctc atccgcgagt cagtaggccc tatctcagtg
     1141 cggtgtcccc aacaaatgtg accatcagtg cgaatcctca agttctcgac gacttcttgg
     1201 ccttcgccaa agtgcaattt cgaaagctct ccatcgagtc tccataccat gcgccacagc
     1261 tatttccgga agttgtttgc ctttctacgg tcaaaaaata tcacgattct cagctcgaag
     1321 cacgccagtt gcatgttccg ctcctgcttc cttctggagc agaatgcgag tctcgaagct
     1381 tcgaagatgt gttgaaggcg agcactcttc acacactcca tgagaaagta cgatgggatc
     1441 gtgttatcga aactgtgtgc actttactgc agcagcgtaa ggctagtgcg tgcgaaatca
     1501 ttcctgtgct gtccaatgcg ccgcagctat tgttcacgca cattcctcgg aagtgtgaca
     1561 tccaaactgt cgtcagcgac ctagagcgat gcgaccaagg atcggcttgg agcaatccaa
     1621 ccggcaagtt tgagcagtcc aagatcgcga tcatcggctt ttcgggtcgg tatcctgact
     1681 cttccagcaa cgacgagttc tgggagctgc tacgtgctgg acgcgatgtg cacaagagaa
     1741 tccccgagga taggttcgac tgggaggcac acttcgatcc cacgggaaag aagagaaata
     1801 ccagcagggt ccagtacggg tgcttcatca aagagcctgg cgttttcgac actcgatttt
     1861 tcaacatgtc tccacgcgag gctgctagta ccgatcctgc gcagcgcctg ttgatcacgt
     1921 ctacgtatga agcgcttgag atggcgggct tcgtgccgaa tcgtacgccg tcaagccagg
     1981 tggaccgcgt tggggttttc atgggcacaa caagcgacga ctggcgcgag gtcaacagcg
     2041 gacaagatgt cgacacgtac ttcatcccgg gcgggaaccg cgccttcatc cccggcagaa
     2101 tcagttattt cttcaagttc tccgggccga gcctcagcat cgatacggcg tgctcttcca
     2161 gcttctccgc catccacgca gcctgcgcgt acttgtggaa gggcgactgt gacacggcga
     2221 tcgcgggcgg caccaacgtc ctgaccaatc cggacaactt cgccgggctt gaccgaggcc
     2281 actttctgtc tacaacggga aactgtaaac cgttcgacga cgctgcggat ggctactgcc
     2341 gtgcagacgc ggttggctgc gtcatcctca agcgatacga ggacgccgtc gccgacaacg
     2401 accccgtcta cggcctgatc gcaggagcgc acacaaatca ttgcggccag gccgaatcca
     2461 tcacgcgtcc tcacgagggc gatcaggcgt ccgtgtttaa tcgcgtcttg agatacacca
     2521 accaagatcc tcgggacgtc agctacgttg agatgcacgg aacaggcacg caagctgggg
     2581 atgccaccga gatgaactcc gtcctgtcat gctttgtcag aggcaagcaa cggacagcag
     2641 agtatccgtt gtacattggc tctgcgaagg ccaacattgg gcacgcagaa tctgcatctg
     2701 gcgtctcctc gctgatcaag gtgttgctaa tgatgaagca caacgaaatc ccgccgcact
     2761 gcggaatcaa ggggaagatc aaccacaatt acccagatct caaggcgctg aatgtccaca
     2821 ttgctttcaa accaacaccg tggacacgcg agggctgcgt cggtggcaaa cgtattgcat
     2881 ttttgaacaa tttcagcgcc gcaggcggca atacggctct tttgctcgaa gacggtcccg
     2941 ttcgcgcaac cccagcaaaa gacatttcaa ggtcaattta ccctgtggca gtttctgcga
     3001 agagtgcgaa atctctcgag cgtaatgtga aagcaatgat tgcatttctt gaggcgaatc
     3061 ctgacactgc tctccctagc ctctcatata ccaccacagc gcgacgcatt catcacaact
     3121 tcagaaccat ggtttctgga acagacttaa agtcaataaa gctcgagctt gacaagatcc
     3181 tgtcaagtct tccagagaag cccattccca atgctgcttt gctgccgaag attgcatttg
     3241 cttttactgg tcagggttcc ttgtacgtgg gagtcacgaa ggagcttttc gatgccgacg
     3301 aagtgttcag gagcgaagtg ctgcgcttgg atatcattgc tcaacagcat ggactaccaa
     3361 gcttcattcc actcctcgat ggaagtgcat ctgcgaggca acttgaacaa cctactatgg
     3421 cgcatgttgg tcacacggcg ctgcagattg ccctaacgaa catgtggtct aggtttggaa
     3481 tttctcctgc tctggtcatc ggccatagcc ttggcgaata cgccgcactt tatgctgcag
     3541 gagtgctgac ggcctctgat gcgatttatc ttgttgggtc ccgcgcgcgt ctgcttgaac
     3601 aatcctgtac tcctcgctcg catgccatga tttcagtcaa ggcttccatg gctgcagttg
     3661 agccaatagt ccaaagtggg tcatcctgcg agattgcatg tgtaaatcag cctgatgcca
     3721 ccgttttgag cggtccgcgg gaagaaatag attgcattat gaaagagctc gaaacactgg
     3781 ggcataaatg catcaaactt gaaattccat atgccttcca ttcggcacag gttgacccga
     3841 ttctggagcc tctgggactt tgtgaaagag ctgtacaatt ttcaaatccg aaaattcctt
     3901 tcctgtcacc aatgatggca gctgtaatca cagaggtgga acctttcaag gatgcatatc
     3961 tgactaaagc atgtcgtaaa ccagtcaatt tccatgctgc tcttcaagct gcgcaggaat
     4021 taggaatagt gggtgacaag actttatggc ttgaaatcgg acctcatcct ttgtgcgcgg
     4081 gaatgatcaa agcgactctt ggctctgact cagtcacaat gccaactctg acaaaacgcc
     4141 agacctcttt ggcctctgtg gttgttgctt tacaagatct ctacatacga ggaattgctg
     4201 ttgactggaa cgagtattat cgacaagacg ctacttcgct tcaagttatt gagcttccac
     4261 agtacaagtg ggatcttaag aactactgga ttcaatataa aaacaacttc tgtctaacta
     4321 agggcgacag cattgagaca aaggatcttt cacgctgtac cgtaccgacc gtcgaaaaaa
     4381 cgattatcac gcctgctgtt caacacatta ttgaaatcag taaggaaaag gcctccattg
     4441 ttactgagac agacatcagc cattctgcac tttccaacgt gattcaaggc cacaaggtca
     4501 atggaattgc attgtgtccg tcatctctct acgcagacat ggccatgact gtttgtgaac
     4561 agttgtgcaa cgcctgcgag atacgcatcg aggaaagcag cttcgaggtc ggtagtatga
     4621 acgtcaaaaa gcctcttatc gcagatggat caactcaatt attgcgcgta tccgcaacag
     4681 ctgatttcga ttcacgggaa attgatttgt ctttttacag tgtttccgcg agtggtacta
     4741 aattagctga ccacgcaact tgtaaagctc gtatcaggag caagggctct cgcgatgact
     4801 ggcaaaggat gtctttcctg atcaaacaca gactggaagc tcttgaagcc agtgttgatc
     4861 atggcaccgc gaataagatg aaacggggta ttgtttacaa gctcttcagc gctttagtcg
     4921 actacagtca aggttatcgc ggcatggagg aggtaactct tgacagcagt gcacttgaag
     4981 ctacggccaa ggttcgcttc caatgtggcc ccgaaggcta cgttctcaac cctcagtgga
     5041 tagattgtct gggccatatc gccggtttca tcatgaacgg aaatgactct gttgcttcca
     5101 gagacacagt cttcgtgaat catggttggg aaaatatgca gatcgcaact aagttctccg
     5161 aggataagac atatcggaca tataacaaga tgcagctgac caccaacaat atctatgttg
     5221 gggacacata catccttgag ggtgacgaaa tagttgccat ttattctggc ataaagtttc
     5281 aaggtgtgcc tcgtcgtgta ctcgaccagt tgcttccgtc tgggcagcgg cgagcaattc
     5341 cagtcaccac aacacccgct cccaaacttc aagaagccaa aatttcaaat gctctttcta
     5401 aggggaagag tatttcatca ccgaagcaca aggtgtcatc tattaaagac acgattctca
     5461 atctaattgc cgaggctgtt ggccttgatg tcaacgaact gaaacccgac acgacctttg
     5521 acgagtgcgg agttgattct ttactctcac tcaatatttc taaagcgatg catgatcagc
     5581 ttgggctgga tatcaactcc agtacatttg cagaatgtcc aacaatcagc tctttcttga
     5641 gccgggttaa agaaaacgaa gcttctccag ggactctaac acctcaatca tccaattcca
     5701 gtactaccga tctcgatagt cttctggatg acaatataag tctcgcgtca tctattacta
     5761 gcgatgaaga cgagaaagag gagccacttg aaaagagcac caacaagact atcgagctca
     5821 tcagatcagt tattgctgcc gaagttggac ttgaaccatc tgaaattggt gattcgacaa
     5881 atttctcaga cctcggcatt gactctttgc tcagccttaa cattatgagt cgtctagagg
     5941 agattatcga gagcgagatt ccaaggatgc tactcgcgga aaatgagaca ctcgagaagg
     6001 tagctcgcgc acttggtctg cagacaaccg tatcgaatgt gactctggaa aaacaaaagc
     6061 agaagctaag caacaatact tccgaaacaa agtttgcagt aaggagtcca ccgcatgctt
     6121 ctagccacct cctgcaaggt aacgcgaaga ctgcgacgaa aaagcttttt ctttttccag
     6181 atggttctgg ctctgccttt tcctacgcaa gcctctcaag gatatccccc tctgtggcgg
     6241 tctatggcct tgcatgtcca tggcagaaag atccatggtc catggccggc gtgtctttct
     6301 ctcagctcag cgccaaattt atcgcagcta tcaaaaagat ccaaccatgt ggaccatatc
     6361 acctcggtgg ctggtctgcc ggtggaatca gtgcgtacga tgctgcgcaa cagcttgctg
     6421 ccgatggcga tgttgtagag agccttgtac tgattgactc tcccaatccc attggactcg
     6481 agaaccctcc acagcggatg tatgacttct ttgacgaact tgactttttc ggcatggaag
     6541 gcaaagcacc accaacatgg ctccgcgctc actttgatgc cttccttaca acgcttgatg
     6601 agtacaagat tgctccatgg aaacaaggaa acagcccagc tacgtacatt gtatacgcaa
     6661 aaaatggcgt gtgcgacatg ctgaagcctg aggatccgcg ccccgagatc agggatgacg
     6721 accctaggga aatgaagtgg ctactgaaca cgcggactga cttttctgcg acaggctgga
     6781 agagccttgt tggagattgc aatgtcagtg ttggggttat tgaaggagtc aaccatttca
     6841 gcatgatgaa taaggggccc aagtcggatg agcttgcaga cttcatcaag agtgcattga
     6901 agatgtaggg agattgataa gacttttcta gttgcatatc ttttatattt aaatcttatc
     6961 tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta aaatatcatt
     7021 tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac agtccaaatg
     7081 ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg gtcgaaatca
     7141 catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac gtcaccgttt
     7201 ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat tcatcgttac
     7261 gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat atctctcttt
     7321 tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta ttcgatattg
     7381 tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc tccagctttt
     7441 gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat atattttgcc
     7501 cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg gcggccactg
     7561 gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg ctggacgcaa
     7621 gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc tacacattac
     7681 cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac gcaggaaaga
     7741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8221 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8521 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8581 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8641 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8701 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8761 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8821 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8881 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8941 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9001 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9061 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9121 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9181 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9241 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9301 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9361 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9421 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9481 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9541 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
     9601 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     9661 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
     9721 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     9781 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
     9841 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
     9901 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
     9961 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
    10021 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
    10081 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
    10141 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
    10201 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
    10261 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
    10321 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
    10381 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
    10441 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
    10501 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
    10561 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
    10621 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
    10681 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
    10741 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
    10801 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
    10861 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg
    10921 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    10981 gacggtgaaa acctcggcgc gccattaccc tgttatccct aatatacaac gcaaagaagc
    11041 tggaaaattg gcgagggaat cctgtttctg tctatccaag aatgggcatg aggtggcaac
    11101 cgtcgtgcta gcgtacaggg tgcactttgt aaccatttgg gacaccggac actcgctgtt
    11161 ttcgaaatta ccctttaagc gcgggtattg aaccaggctt atgcccaaga tcgtagcaag
    11221 catttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac
    11281 cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
    11341 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
    11401 ctgaaaaatt gggagaaaaa ggaaaggtga gaggccggaa ccggcttttc atatagaata
    11461 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
    11521 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
    11581 cttttacatt tcagcaatat atatatatat ttcaaggata taccattcta atgtctgccc
    11641 ctatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca
    11701 cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg
    11761 atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtc ccacttccag
    11821 atgaggcgct ggaagcctcc aagaaggttg atgccgtttt gttaggtgct gtggctggtc
    11881 ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac
    11941 ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat
    12001 ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg
    12061 gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg
    12121 aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac
    12181 aacatgagcc accattgcct atttggtcct tggataaagc taatcttttg gcctcttcaa
    12241 gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca ttgaaggttc
    12301 aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg
    12361 gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc
    12421 caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg
    12481 catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggttg
    12541 accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg
    12601 aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg
    12661 gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta
    12721 agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa
    12781 atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac
    12841 gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag gatagtaaag
    12901 gaatacaggt aagcaaattg atactaatgg ctcaacgaaa tgacggtatc gataagcttg
    12961 atatcgaatt cctgcagccc gggggatcca ctagttctag agttaattaa
//
