LOCUS       sequence-21788-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Jin J, Cai Y, Yao T, Gottschalk AJ, Florens L, Swanson SK, Gutierrez
            JL, Coleman MK, Workman JL, Mushegian A, Washburn MP, Conaway RC, 
            Conaway JW
  TITLE     A mammalian chromatin remodeling complex with similarities to the 
            yeast INO80 complex.
  JOURNAL   J Biol Chem. 2005 Dec 16. 280(50):41207-12.
  PUBMED    16230350
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Dec 16. 280(50):41207-12."
COMMENT     Sequence Label: FLAG-mTIP49a
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             505..513
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcagagctct ctggctacta gagacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcat gccagactac aaggacgatg atgacaagct
      121 cgagatgaag attgaggagg tgaagagcac cacgaagacg cagcgcatcg cctcccacag
      181 ccacgtgaag ggtctggggc tggacgagag cggcctagcc aagcaggcgg cttcgggtct
      241 cgtgggccag gagaacgcga gagaggcatg tggcgtcatt gtagaattaa tcaaaagcaa
      301 gaaaatggct ggaagagctg tcttgttggc ggggcctcct ggaactggca agacagcctt
      361 ggcccttgct attgctcagg agctgggcag taaagtccct ttctgcccga tggtgggtag
      421 cgaggtatac tcaactgaga tcaaaaagac agaggtgctg atggagaact tccgcagggc
      481 cattgggttg cggataaagg agactaagga ggtttatgaa ggggaggtga cagagctcac
      541 tccctgtgag acagagaacc ccatgggtgg gtacggcaaa actatcagcc atgtgatcat
      601 agggctcaag actgctaaag gaaccaaaca gctgaagctg gaccccagta tttttgaaag
      661 tttgcagaaa gaacgagtag aggctggaga tgtgatttac attgaagcaa atagtggagc
      721 tgtgaagagg caaggcaggt gtgacaccta tgccacagag tttgaccttt gagctgaaga
      781 gtacgtccct
//
