LOCUS       sequence-217880-       15017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15017)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 15017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU64.1-2.2
FEATURES             Location/Qualifiers
     source          1..15017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9452..9472)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9452..9470)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9809..10397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9889..9908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10568..11359,11360..11428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11191..11210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11429..11533)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11560..12902
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12523..12570)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12943..12961
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13233..13637
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13650..14744
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14992..15008)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14992..15008)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgggta
      541 gctatccaga gaccaacctc ctctccatcc cagagcccat cgccattgta ggaacctcat
      601 gtcggtttcc aggtggtgca gactctccat ctaaactatg gagtctcctc aagaaccctc
      661 aagacattgt gcaagagatt cctccgagtc gcttcgacac gaaagggttc tacaacaaag
      721 acagccaaca ccacggtagc ttgaacgcac gacacgcata tcttttgcaa gatgatcccc
      781 gagcattcga ccgcgacttc ttcaacatca gccccaagga agccgaggcc atggatcccc
      841 aacaacgctg tctcctcgag acagtctatg aaggcgttga atcggccgga tactcgatac
      901 cccagctgca aggctccgcg acaggtgtct ttgtaggcgc catgagcttc gactaccaat
      961 ttgttgctat gcgaggcttc gatagcttgc cacagtacca cgcaaccggt acttccatgt
     1021 cgatcctggc gaatcgcctc tcctacttct ttgactggag agctgttctt gcgctgcgga
     1081 acggtgatgt tagtatggct gttgctgccg gcgctaatct tattctcggg cctgagccgt
     1141 ttatcgccct gtcaaacctg acgaaggaat tgaagttaaa catgctgtca cccaacggcc
     1201 gttcatacat gtgggattca tccggcgacg gctacacacg aggcgaaggc ttcgctgccg
     1261 ttattctcaa gactcttagt caagccctag ccgacggtga ccacattgaa tgtatcatcc
     1321 gtgagactgg cgtaaactcg gatgggcgga ctccgggcat caccatgccg agcgcaacag
     1381 cgcaggctcg gcttatccga gagacatacc aaaggtgtgg gctagacata aacagtccac
     1441 ttgatcgccc acagtacttt gaggcccatg gtaccggcac accggcagga gatcctatcg
     1501 aggcccgagc tatacatgaa gctttctttt ctcatagtcc ggaaccccat gacgaccagg
     1561 caccactcta cgtcggctcc atcaagactg ttcttggcca cactgaaggc actgccggcc
     1621 tcgctggagt ccttaaggca tctctggcag ttcagaacgg ttcaattcca ggaaatctcc
     1681 attttaaaaa cctgaatccc aagatctggc ctttctacga caaactgcgc gtccccacca
     1741 cattaacgtc atggcctagc cttccagacg gccagcctca ccgcgtcagc gtcaacagct
     1801 ttggctttgg aggaaccaac gcgcacgcca ttatcgaaag ctgggatgag aagcccgagc
     1861 ggtgtccttt gagaactgca gaacatggcg gacttgttgt gttatctgcc aattccgcac
     1921 aggcattggc agcaaaggct acgtcgctgg ctgcatacct tcgaaagcac cctgatacag
     1981 atatcgaccg gctctcacgc accatgttcc agcgcgctga tttcctatac cgagccgcct
     2041 ttgcagccac gtcgactgcc caacttgcag agaaacttga agcacgaact gagatgttga
     2101 aaaagacttc ccgcattgcg gcaattccta accacttgcc gccccgcatc cttggtgtct
     2161 ttaccgggca aggcgcgcag tgggctacta tgggcaggga actgtacgat gcatcgcctg
     2221 tgttccgcaa atccatggac cggctgcagc gaagccttga cacgcttcct catggcgaac
     2281 gcccagactg gactcttatc gacgagcttt cgctgtccaa agagacgtct cgtattggtc
     2341 aagccgccat ctcgcagcca ctgtgtactg ctttgcagat cgctctcatc gatgtcttgc
     2401 acgtcatcgg catctccttt gctgccgttg ttggccactc ttctggagag attgcctgtg
     2461 cctatgcagc agggtaccta gatgcacagg acgccatccg cgttgcttat taccggggat
     2521 tccactcaca cctctccaag ggccccgacg agaaacgcgg caaaatgatg gctgtgggac
     2581 tgagtctcga gcaagcgtca gccttctgta gtgagttcgg gtctgcgctc aaagtggctg
     2641 ctagcaactc tcccaccagt tgcacactgg ccggcgatgc ggacgccatt gatgaagcca
     2701 aagagcggct agagcaggaa aagacttttg ctcgtgtgct agctgtagac accgcatacc
     2761 attctcatca catgcttccc tgcgccgaac cctatctaca gtcgctacgc aactgtggag
     2821 tccgagctct caggggtcca aagcgatgtg cttggtactc cagtgtctgg ggccccaatg
     2881 gacgcagccg ctctttcgat agcaaagata gcatggagtt gctcgaaggc cagtactggg
     2941 tcgataacct aaccaacacg gtccagttct cgcaagcagt ataccgagct atttccgaag
     3001 agcccttcat catggacctc gcactggaag ttggacccca ccctgctctc aagggaccca
     3061 gctctgaagt catgaagtcg ctgactgggg tttccgttcc atatagcggt attttgaaac
     3121 gcggagagaa tgccgtcgaa gcgtttgccg atgctctagg actcatctgg acgtctttcc
     3181 catctcagaa ccctatcgtc acgttcgatg ggatgcatcg cgccttgcca caagctggac
     3241 caaagaagcc tagcatcctg aagaacttgc caggttaccc atgggaccat gatagcctca
     3301 tctggcgtga atcgagggct tctcgcgtgt tccgctctca gaaccagcca agacacgagc
     3361 tcctcggtca ccctgtaacg ttgggcgaac atagcaggcg agaggtacac tggcgccaga
     3421 tcttcaagct gggcgagctt ccttgggtga ctggtcataa tatccaaggc gaagtacttt
     3481 tcccagccac ggggtacctc acaatggcgt acgaagccgc tatacgcctt gtagacaatg
     3541 agacagcgct gcgcctcgtc gagttgcacg atgtggagat tgttcgcgct atgggtttgc
     3601 cagaagattc ccccggcctc gaggttctct tcactgtgtc cgttacaggc cagtctgacg
     3661 actgcgtcac ggcaaaagtg gcttgctaca gcggcaacgt caacgtagcc aagctggata
     3721 gtcctcttca tggactcaca gctcatttct cagctggcgt tagactttgg ctcggtgaac
     3781 ccagcaatag ttccctacct ggccgcaaca gacccctgct ccccatgaat accctcgata
     3841 tggagcagtt ctactcctac ctgtcgaagg taggatacaa ctacgcgctg cctttcagag
     3901 caaccagtgt ggaccgccat ctcaaccacg cggtcgtgac gcttccatcg cctccagaga
     3961 cgtcttcgtc tattcgtgat gttacgcatc ccgtagtcct cgacactgcg attcaaggag
     4021 tcttggccgc attttctttc cctgaagacg gccgcctgga cactgtgtat ctccccacac
     4081 gcatcgactg cgtccgcatc aacacatcgt caacatataa tagccccttg atggcagaca
     4141 gcgtcttgac atcgaccgaa gcaaaggcac tcaagggcga catcgaggtg ttcagcgaga
     4201 gcgacgcagc cgtccacgtg caaatgcgtg gcgtccacta cactgcgctg aaccaaaaca
     4261 aagaccgatg gctgtacgca agcgagaact gggtgcgcga tgcaatccac ggtattgagc
     4321 cgagccagat gaccaagctt tctcctgaag ccgaggcgtt tgagaagctc ctgacacgca
     4381 tagcgtattt ctatctgcgc aagctccgcg atcagatcaa gccgtcggag ctattgctca
     4441 tgagcaagca ccgacgccac atgatgaagt gggtcagaga gcatctgttt ccccagatcg
     4501 aggcaggcca gcgtccagat gtcgaagtgg aatgggtaaa cgacaccctg gaggatgtcg
     4561 agcggtggag cgctagttac cttgcagctg gcaacaacga catgcaactg cttcatgccg
     4621 tcgggaagaa tctgcctgct attgcccgcg gaacgaagcc tgcgctacag gtgttgctga
     4681 ccgacgacat gctggaccgt ctgtacgtcg aaggcactgg aatcgcggat gctaacctcg
     4741 actttgccct gttggtgcag cagattgcac accgctaccc gcgcatgaat gtcatcgagg
     4801 tgggtgccgg aacaggcggt accacgcgag cggtgctgtc ctctttgggc gatcagtatg
     4861 cgtcgtacac ttataccgac atctcagccg gatttttcga gcctgcaaag gcaaaattca
     4921 gccagcacgg cggaaagctc aagttcaaaa cactcaacat tgaaaaggac cccatgggcc
     4981 aaggctttga tgaaggctcg tttgacatgg ttattgcttc aaattgtctc cacgctactc
     5041 gcagtctcca ggaaacactg cgccactgcc gccgcttgct ccggcccgga ggctacttag
     5101 tcctgctgga aatcactcgc gaccatctcc ctattgagga gtgggacgcg ttgctcaagg
     5161 caaacaggtt ctctggagta actgcaagct cgacgccttc ttattgctct gtgataatgt
     5221 cgcaggcagt tgatgatacc ttccagctac tccaggggcc gctctctgtc acttctcagc
     5281 agcagccgtt ggcatccaga ggaccggtat tggttgttgg tggtaacaag tcaggacttg
     5341 gtgccaggtc tcaggaaata cttgcttctc acggtgcatc atacctgcat gagcttgagg
     5401 gcatcgaggt gccgagtgga gcagtcgttc tctgtctatc tgacttggac aaccccatct
     5461 ttagtgtcat gacagaggct gcgttccagg gtatacaaac aatcttccgg aacgcaagca
     5521 ttgttctgtg ggtgacgtct ggagcggctt cgggcaaaaa tccaatggcg aacgtaacgg
     5581 tcggattagg acgaacactg cttgccgaat gcagtgactt gagactccag ttccttgacg
     5641 cggaagagcc tgattcactc actccggaat tgctggcgac tcttctcctt cgacttggcg
     5701 caacggacca gtccaactcc gatgatatcc tctggaatca tgagcctcat cttgcactga
     5761 gagacggcgc gttctacgta cccagagtgc tgcgcctaga cagcttgaac cgccgcgcta
     5821 gtgcgaagaa ccgaaagctc acccagagta ccagtcttga gctctctgat actggcgtcg
     5881 agatgtctca acaagacgga gccttggagc tgttcgatta ccctgtcggc gattgtaggc
     5941 caagcgagat tcgtgtacag gtcatggcat catccatcca tgcttttaca ctcgatcgcc
     6001 cggcgcccgt ttacttctgg attggtcacg atgtccattc taaagacaga gtcatgggct
     6061 tttcggaatt caaccgctcc ataatcacgg ctacggaaga tcagatcttt taccgtggag
     6121 acaatgtcag cacacaagac aagcgggatt cagatgctga gcagctgcac cgattcatcg
     6181 tacaagcgat ggctgcatct cttctcggta attttgaggc acccatctgg gtacacggcg
     6241 ctccaaacca cttcaagccg gccattactc gtgctgccca cgaagccaat gtctcagtgt
     6301 ccctgacgac atcagatatt gcaagcgctc aagacgaggg tttcatccac ccgtacatca
     6361 gcaagcgaga tcttcagtca cttgtcccaa gatgcgtcaa aacactgatc aacctggagg
     6421 ccgcgcaaaa cgaggatctc tcgaagctaa tgcgagattc cctgccttct tctaccaagg
     6481 taatccaggg tgatgatctg acgattccga tcaggtgcga gaagctgcgc gagctagccc
     6541 aggttattgg tcatgacgaa atcaagggcg caagtcacgc agaccatgtc acatccattg
     6601 gagagctgac agaagacaaa gtcaaagcat tgagccaagg ggctgtcata gactggcgca
     6661 ctaccgatag cgtcaccacg actgttcgac ctctggatca aagcgggctc ttcgatgcca
     6721 acaagaccta catccttttc ggcatgacag gggacgtcgg catatctgtt gctgcatgga
     6781 tggttgacca cggcgctcga aacgtagtgc tgggtagccg aaaccctagg gtaccagagg
     6841 gcgtcattga atacatggct cgaaaaggag ccaacctgtg cgcagtcaag gtcgacgtga
     6901 ccaagaagga agagctgcga gaagcgtgtg ccaacatcaa ggcaaccatg ccgccgattg
     6961 cgggtgtcat caatggcgcc atggttctcc gagacagact tttcgcccac atgtcctggg
     7021 acgactttga agctgtcctg gcgccaaagg tcgctggaac tcagaacctg gacgagattt
     7081 tcagcaacga acaagatgcg ctggatttct tcattgttct ttcgtcggca acatcgctgg
     7141 taggcatcat aggccagtct gcctacagcg cagcgaacca ttttatgtcg agtctggtgc
     7201 gccagcgaca tgctcgtggt ctagctggtt ccgttgtggc aatcgggttc ctgacgggct
     7261 tgggctacat tttccgctcg gaaaaggagc atctcgatgc tattgagaga caagccgaga
     7321 cagacctgca cgacatgctc gcggaggcgg ttgtgtgcgg tcgacccaac tctggtcagc
     7381 cgtctgagct catcaccgcc atcaagacaa cattcccgga tgcgtggcac gaagacccac
     7441 gcctctcatg ctaccttgta caggatagcg tggaagaagg cactgatgcc aaagaagtcg
     7501 agggcaatgc caaggtcgaa gctcaattgg catcggccga ggatccaaag cagtgcttga
     7561 cgattctgat aaagtgcttc agcgtggcgc tgggcaacat gctccagctc gacccggctc
     7621 agatagcagt cgacgtccct gttgtcgatc acggcgttga ctctctggtt tcggtccgta
     7681 tccgcgaatg gttcctcaaa gagctcggcg tcgacgtgcc ggtactaaag ctcatgtcga
     7741 ccaaccactc cctgtcgcgc gtatgcgagg atgcactagc aggatggcgg aagcttcgtg
     7801 cagggccccc aacagcgtcc cagacagagg ccaaggacaa cgagcctcgc cgcgacccag
     7861 aaaaggactg gaaaaaggag gtggccgagc tcattagcgg aatccgaggc ctcatcccgc
     7921 gaggcactga tgcaatggcg agcaccgagc cgcctcggac aaacgctcgc cgggtcgttg
     7981 ttacaggatg tacagggttc ctgggcactc acctcctgcg ccatctggtc gccgatgcga
     8041 gtgtcgctga ggtacattgt ctgtgtatcc gctcacgcca tgtacgcgtc caggacgcca
     8101 aaatccgtga atacgccggc gacctagcca agccgctgtt gggactctct gctaccgact
     8161 tcaagcgcct cagtcagaca gcagacgtga tcatccacct gggtgccgat gtcaaccatc
     8221 tcaagtcgta cgaggcgatg cgtgctgcca atgtggtgtc gacacagaca ctgcttgcca
     8281 tggcgatgcc gcgcagcgtc cccgtgcact acatctcatc ctcgtcggtg gccatgctgc
     8341 agaagggaac ttcggaactc gcagaagtgg cgccctcgag catctcaccg ccacgtgacg
     8401 tcgattcgtt gatgaagagc gccgtgggtt acgccgccag caaatgggtg ggtgggatgc
     8461 tgcttgaaaa cgtcgagggc cctccaacgg tggtgcatcg ctttcccaac atcatgggcc
     8521 atgatgcccc tgaagagatt cccttggtag ctctcgatcg ctactgcacc aagatgcggg
     8581 ccgtaccagc gctggatccc cgcttgtgga tcggccagct cgatgtcatc gatgttggcc
     8641 aggttgtccc ggactttgtg gaaatggcgt ggagcctgga ttcccgtgaa tcattcgccg
     8701 tacacaacta ctgcagcaat aacagttact tgctttcgga tcttgcggga atgtacagag
     8761 agagactcgg ggccaatgtt gaggttctgc cgacgcctga gtggatgcgc agggccatcg
     8821 cgatgggtat gcccaagggt gttgaagcca catttgtggg ccatgacgaa gtgtttgtgt
     8881 cgcctgtttt gcgcaagggt ccggccaaga aatagggaga ttgataagac ttttctagtt
     8941 gcatatcttt tatatttaaa tcttatctat tagttaattt tttgtaattt atccttatat
     9001 atagtctggt tattctaaaa tatcatttca gtatctaaaa attcccctct tttttcagtt
     9061 atatcttaac aggcgacagt ccaaatgttg atttatccca gtccgattca tcagggttgt
     9121 gaagcatttt gtcaatggtc gaaatcacat cagtaatagt gcctcttact tgcctcatag
     9181 aatttctttc tcttaacgtc accgtttggt cttttatagt ttcgaaatct atggtgatac
     9241 caaatggtgt tcccaattca tcgttacggg cgtatttttt accaattgaa gtattggaat
     9301 cgtcaatttt aaagtatatc tctcttttac gtaaagcctg cgagatcctc ttaagtatag
     9361 cggggaagcc atcgttattc gatattgtcg taacaaatac tttgatcggc gctatgcggc
     9421 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     9481 cgagactcaa acaagatata ttttgcccgc cttacagacg aaactagttg gaggttatgg
     9541 agcatactat cacgtgggcg gccactggtg agttactaca ccccaggggc aacgttgatg
     9601 ctcctaaaaa actctggctg gacgcaagcc gtaacacccg tgtcacttca taatcgtttg
     9661 caattcaggg cttgatctac acattaccct gttatcccta ttaattaata cggttatcca
     9721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    10261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10441 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10501 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10561 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10621 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10681 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10741 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10801 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10861 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10921 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10981 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11041 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    11101 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11161 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    11221 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11281 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11341 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11401 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11461 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11521 taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat
    11581 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
    11641 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
    11701 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
    11761 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
    11821 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
    11881 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
    11941 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
    12001 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
    12061 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
    12121 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
    12181 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
    12241 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
    12301 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
    12361 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
    12421 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
    12481 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
    12541 agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa
    12601 aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt
    12661 tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt
    12721 acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc
    12781 ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc
    12841 tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga
    12901 tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    12961 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tcggcgcgcc attaccctgt
    13021 tatccctaat atacaacgca aagaagctgg aaaattggcg agggaatcct gtttctgtct
    13081 atccaagaat gggcatgagg tggcaaccgt cgtgctagcg tacagggtgc actttgtaac
    13141 catttgggac accggacact cgctgttttc gaaattaccc tttaagcgcg ggtattgaac
    13201 caggcttatg cccaagatcg tagcaagcat ttaactgtgg gaatactcag gtatcgtaag
    13261 atgcaagagt tcgaatctct tagcaaccat tatttttttc ctcaacataa cgagaacaca
    13321 caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag
    13381 gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag
    13441 gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
    13501 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
    13561 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatttc
    13621 aaggatatac cattctaatg tctgccccta tgtctgcccc taagaagatc gtcgttttgc
    13681 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
    13741 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
    13801 tcgatgctac aggtgtccca cttccagatg aggcgctgga agcctccaag aaggttgatg
    13861 ccgttttgtt aggtgctgtg gctggtccta aatggggtac cggtagtgtt agacctgaac
    13921 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
    13981 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
    14041 acttcgttgt tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg
    14101 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
    14161 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
    14221 ataaagctaa tcttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
    14281 agaacgaatt ccctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc
    14341 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
    14401 tcatctccga tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct
    14461 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg
    14521 ctccagattt gccaaagaat aaggttgacc ctatcgccac tatcttgtct gctgcaatga
    14581 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa
    14641 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
    14701 tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt
    14761 tatgatattt gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac
    14821 aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat acatttatca
    14881 agaaggagaa aaaggaggat agtaaaggaa tacaggtaag caaattgata ctaatggctc
    14941 aacgaaatga cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta
    15001 gttctagagt taattaa
//
