LOCUS       sequence-217881-       20508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20508)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 20508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU74.2-2.2
FEATURES             Location/Qualifiers
     source          1..20508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1188..1199)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2079..2378
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4990..5210
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5211..6014
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      12624..12811
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(12679..12703)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      14418..14667
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(14434..14452)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(16655..16675)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16655..16673)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(17012..17600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17092..17111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17771..18562,18563..18631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18394..18413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18632..18736)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      18763..20105
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(19726..19773)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     20146..20164
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(20483..20499)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(20483..20499)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggcgacag tgcatcttca cgatgagttg acgcaatcta
      541 cggaacgggt cacggtccag acgcgctttg gcacgatcag aggcgggcgt gctgcgaatg
      601 gcgccgcggt cttcttggag gttccgtacg ctctcccccc ggggaggttt ttggacccgg
      661 tcccattgcc cgcggactat cagtatgagg agaaggatta catctacgaa agcagctacg
      721 cggctcaacc gattaatgac ggtcaggcag ctagtgtgcc attcgaggac aaagtcggac
      781 gaggcgaacc cactgagaac cctctgttcg tcaacatagt atccccgccg tcgtttccct
      841 ctgcgactgg cttgcctgtg aaagtgtaca tccacggcgg cttcttgcag tttgggtcgc
      901 ctcacggtct aagtgggcaa gcgcaataca ttgcggccga gcgatcggaa atatgggtca
      961 acgtcggata ccgcctttcc gcgtttggct tcctggcatg cgacgagcca cgcgtcgaag
     1021 gaaactttgg tttcaaggac cagtggttgg cgctgctatg ggtccgtgac aacatcgagg
     1081 cattcggagg caatccagac aacatccaaa tcagtggtct ttccgcgggt gcccattctg
     1141 tacatcagct tctgcattac gcttcgaggc tgccagaggg caaaacggcg cccttcaatt
     1201 ctgcagttct gcagtcgaac tccattgtaa tagccccgaa aacccccgcc gagctgcgcc
     1261 cgcaattccg cgcgttctgc accgcgctct ccctcaaccc ggacggcccc gacgccttaa
     1321 ccgtcctgcg cgacccggag ctggtcccct ggaaggccat cacgcacgcg atcgaaaccg
     1381 acgcgctcgg gacccagttt ggcaccttcc gcggcagcct cgacggctcc tggctttcct
     1441 ctgatccgga atcaatggcc tggcagcgct ccggcgcgtt cgcccgcgcg ttgcgaaagc
     1501 gtggtgtgcg gagcatcgcg gtgggcgacc tcacggagga atggtacctg tactcgatcg
     1561 cgcaccccgt gcggcgcatg cgtgatattg agctgaacat ggagcggtat taccaggagg
     1621 aggtcgtcca cgccatgatg gcgctgtaca ggcgcgtccc cgacggcgcg cctcccgagg
     1681 attttgcgcg cctgtttggg gagatgctga gcgaaggcca ggtgcatttg cctgtgcggt
     1741 tgctcgcgag ggacctcacg acggctgggt tcccggtggt gcggtaccag attcgatgga
     1801 cgcccgagca gctgaggcct ctcggatatg tcacgcatgg aagcgaccgg tcgctctggg
     1861 cgcttcgtct acccgtgctc aagccagacc aggccgatgt tgcgcgtgcg tggctggaca
     1921 gcatcaattc cgcactcgct gcggcggaga acggggaggt acgagggctg aaggaggttc
     1981 tggctttgaa tgaaaaccaa agtatcggat ggagagacga tgacaagtgg gacgagatca
     2041 tgcgcctttg tcaggcgttg ccgggggagg atgcataagc ggatctctta tgtctttacg
     2101 atttatagtt ttcattatca agtatgccta tattagtata tagcatcttt agatgacagt
     2161 gttcgaagtt tcacgaataa aagataatat tctacttttt gctcccaccg cgtttgctag
     2221 cacgagtgaa caccatccct cgcctgtgag ttgtacccat tcctctaaac tgtagacatg
     2281 gtagcttcag cagtgttcgt tatgtacggc atcctccaac aaacagtcgg ttatagtttg
     2341 tcctgctcct ctgaatcgtc tccctcgata tttctcattt tccttcgcat gccagcattg
     2401 aaatgatcga agttcaatga tgaaacggta attcttctgt catttactca tctcatctca
     2461 tcaagttata taattctata cggatgtaat ttttcacttt tcgtcttgac gtccacccta
     2521 taatttcaat tattgaaccc tcacgatcca gttctccagt gacacagcct ttatctggtc
     2581 aaacctttct ttctaatcac ctatgctgat gcttaattaa gggatttttg tctccatcaa
     2641 cggcatgcgc ccaaaaatga cgtttttttt aacccataga cacgaaacta cccattttcc
     2701 accggcctga cctaccaccg gaacaacggc catctccaac ttgcaagttg gggaaattaa
     2761 gagcatcgca ggtttaatgg aagaaaaaaa aaaggtacag cacagcgcaa atggagttag
     2821 ttcccttatg tcacacactc acacacagtc ggtcagatca agcatactgg gtgcgtataa
     2881 atagagtggc cattgccacc ctgtttatct caaaatctgt cttgttagtg gtcttctccc
     2941 tttttcaggt tacaattctc ttgtttctac ttagtatata agtatatcaa gctatattaa
     3001 gcatactatc aactgtcaac tctatcctca aaatacaata caaaatgttc aacttactgg
     3061 actccgccaa aggctcgcac gcaacgacgt tcctcgtcgc ctcacttgtt ttcctcgtct
     3121 tgaagcagtt cttcacctcg cgaaagatgg ccaagcgagc accgggtccg acgcgctggc
     3181 cattcctcgg caatgctctc caacttcctc aacaacagat gtggctgacc ttctcggaat
     3241 ggcaaaagac atacggcgac attattcacg tcgacgtctt caaccagccg atcatcatta
     3301 taaactcctc gaagatagcg aaggatctct tggacaagcg gtcctccatc tattccgacc
     3361 gcccgcatct ggtcatggcc ggtgatttag tgggctacag cgacccgttt gtcctgcaac
     3421 cttacggaga aaagtggaaa aagcagcgca aatttgtcgc atcagacttc agtcccgcca
     3481 ccatgccacg gtattaccag ctccaagaga ccgaggttcg caaactggtc cgcaatgtca
     3541 tcttggaccc agattccctc ttctcgcaga ctaaattgcg gattgggaca attatcatcc
     3601 gggttaccta cgggtactac atcaagggcg agcaggaccc gttcctgact ttgcctttga
     3661 ctgcgatgga aaacttcagc aaggcatcgg ctccagggac atggctcgtt gacttcgtgc
     3721 cgcagatgaa gaacatgccc tcctggatgc ccggcgcggg ctttctcaag acggccaaga
     3781 aatggcgcaa gatcgtctgg gatgcgtcct gggacccata caactggtgc aagaatggcc
     3841 ttgaaaccgg agagacgctc atgccaaacc tctgcgggag cgctatccag tccgccgcag
     3901 atggaaagct ctccaaggag gacgaggagc agctcgtatg ggccgcgtcc gcaatcatgg
     3961 gcggcggcct cgacacgaac atgtccgcgg cgttgagctt cttcctcgcg atggtgctgc
     4021 atcccgccgt gcagaggaaa gcgcaggccg agatcgacgc ggtgatcggc tgcgaacggc
     4081 tcccgtccat cgcggaccgc gacgcgttgc catacgtgcg cagcatcatc acggaggtcc
     4141 tgcgctggaa gccgcccgtc ccgctcagta tcccgcactc gctcacgcag gacgacgtgt
     4201 acgagggcat cttcctgccc aagggcgccc tcatcatgcc caacgtttgg cacatgatgc
     4261 acgaccccac gtcctaccca gatccctccg ccttccgccc cgagcgctac gcgggtgcgg
     4321 acggtgacgc agcgcagcgc cgcgtgcacg agcttgtgtt cggcttcggc cggcgtgcgt
     4381 gccccggcgt gcacttcgcc gagggcacgc tcttcgcgct tgtcgcgacc gtcctcgcga
     4441 ctgtcgacgt ccttcctgcg ctcgacgcgc agggcaggga aatcaggccc gacccgaagt
     4501 actcgtctgg tacgatcacg ttcccggagg cgttcccggt gcggttcagt gtgcgttcgg
     4561 agcaggcgga aacgctccta gcgcaggttc cggcgggtcc tatgtgaaca aatcgctctt
     4621 aaatatatac ctaaagaaca ttaaagctat attataagca aagatacgta aattttgctt
     4681 atattattat acacatatca tatttctata tttttaagat ttggttatat aatgtacgta
     4741 atgcaaagga aataaatttt atacattatt gaacagcgtc caagtaacta cattatgtgc
     4801 actaatagtt tagcgtcgtg aagactttat tgtgtcgcga aaagtaaaaa ttttaaaaat
     4861 tagagcacct tgaacttgcg aaaaaggttc tcatcaactg tttaaaagga ggatatcagg
     4921 tcctatttct gacaaacaat atacaaattt agtttcaaag atgaatcagt gcgcgaagga
     4981 cataactcat tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
     5041 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
     5101 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
     5161 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
     5221 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     5281 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     5341 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     5401 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     5461 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     5521 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     5581 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     5641 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     5701 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     5761 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     5821 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     5881 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     5941 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     6001 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     6061 gagcttcaat ttaattatat cagttattac cctatgcggt gtgaaatacc gcacagatgc
     6121 gtaaggagaa aataccgcat caggatagga aaaaaccgag cttcctttca tccggcgcgg
     6181 ctgtgttcta catatcactg aagctccggg tattttaagt tatacaaggg aaagatgccg
     6241 gctagactag caagttttag gctgcttaac attatggata ggcggataaa gggcccaaac
     6301 aggattgtaa agcttagacg cttctggttg gacaatggta cgtttgtgta ttaagtaagg
     6361 cttggctggg gatagcaaca ttgggcagag tatagaagac cacaaaaaaa aggtatataa
     6421 gggcagagaa gtctttgtaa tgtgtgtaac ttctcttcca tgtgtaatca gtatttctac
     6481 ttacttctta aatatacaga agtaagacag ataaccaaca gcctttccca gatatacata
     6541 tatatcttta tttcagctta aacaataatt atatttgttt aactcaaaaa taaaaaaaaa
     6601 aaaccaaact cacgcaacta attattccat aataaaataa caacatgcgt atcattgtta
     6661 caggtgcatc tggactcctt ggctcccaga tatatgctgc attcaagaat acggaacata
     6721 cggttctggg ccttgcgcac tcacgcgcga caggagagct gcagaagcta gatctcttgg
     6781 atagcaagga ggtcgagaag gtcttcgcag atttcaaccc ggaatgggtg atacactgtg
     6841 cggcggagcg caggccagat gtagcggaga agaaccccga cgggacgcgg aaacttaacg
     6901 tcgaaatccc ccttcacctt gcacaattgt ccaagtccct caagtatacg ctaatctaca
     6961 tctctaccga ctatgtgttc gatggaacct caccacccta cacccctggt gcactcacga
     7021 accctgtcaa cctctacggc cagacgaagc gcgacggtga gctcgctgtc ctcggcgtat
     7081 ctgacgcgcg tgcggtcgtc ctgcgtgtgc ctgtgctcta cgggcctacg cccaacaata
     7141 ctgactcggc ggtgaacatc cttctggaca tcgtcgcgga ccagtcgggc aagacataca
     7201 agatggacca ctacgcaacg cgcttcccga cgggcgtcgt cgacatcgcg gatttcctcg
     7261 tgaggctggc tacactcccg ccgtcccgac agatcccacc gatcctgcac tactctgcga
     7321 gtgagccgtt caccaagtac gagatgtgcc tcatcttcgc gcgtatcctt ggtgtgccgc
     7381 attcgcacat cattcccgac gcagaggctc ccaagggtga tgcagcaacg tcgcgccctc
     7441 gcgattgtca gctgtatacc cgcgaaacgg aagacctgat ggagggatac ggcggtctcg
     7501 gctggacgcc attcgaggag tggtgggtcg actatctaaa caagaggcag tgaagtgctt
     7561 ttaactaaga attattagtc ttttctgctt attttttcat catagtttag aacactttat
     7621 attaacgaat agtttatgaa tctatttagg tttaaaaatt gatacagttt tataagttac
     7681 tttttcaaag actcgtgctg tctattgcat aatgcactgg aaggggaaaa aaaaggtgca
     7741 cacgcgtggc tttttcttga atttgcagtt tgaaaaataa ctacatggat gataagaaaa
     7801 catggagtac agtcactttg agaaccttca atcagctggt aacgtcttcg ttaattggat
     7861 actcaaaaaa gatggatagc atgaatcaca agatggaagg aaatgcgggc cacgaccaca
     7921 gtgatatgca tatgggagat ggagatgata cctccattgg gccgatgaag ttagtcgacg
     7981 gatagaagcg gttgtcccct ttcccggcga gccggcagtc gggccgaggt tcggataaat
     8041 tttgtattgt gttttgattc tgtcatgagt attacttatg ttctctttag gtaaccccag
     8101 gttaatcaat cacagtttca taccggctag tattcaaatt atgacttttc ttctgcagtg
     8161 tcagccttac gacgattatc tatgagcttt gaatatagtt tgccgtgatt cgtatcttta
     8221 attggataat aaaatgcgaa ggatcgatga cccttattat tatttttcta cactggctac
     8281 cgatttaact catcttcttg aaagtatata agtaacagta aaatataccg tacttctgct
     8341 aatgttattt gtcccttatt tttcttttct tgtcttatgc tatagtacct aagaataacg
     8401 actattgttt tgaactaaac aaagtagtaa aagcacataa aagaattaag aaaatgctcc
     8461 cctcgctgag aaactacctt cggtttattg atctcgagaa cgaattcgcg gatcatgggt
     8521 ttttgccaaa gcctggagcc ggcttcaagc ttgtgcaagg aatccgagag agctggactg
     8581 acttcaccgc tctcggaccc ggttacggta catggaacgt gatacgctcc gagatggacg
     8641 acatgatgct gcggcatgca tccaagcaag gcgtcaaggt cttcgaagaa acccgtgtcg
     8701 agaccctcga cttcgcaggg gaccgcgcgt cttcgcgccc gctcgctgcg cactggtcga
     8761 acaagcacgg cgcagcgggg cgcatcacgt tcgactggct cgtcgatgcc tcggggcgga
     8821 gcgggatcat gtgcacaaag tacctcaaga accgcacgat gcgcgagagt ctgcgcaatg
     8881 tcgcggtgtg gggctactgg aagggcgtga cgcgctacgg cgagggcacg aagcgcgcat
     8941 gctcgggctg gttcgaggcc ctgacagatg aacaagggtg gtcttggacc attccgttga
     9001 atgacgggac aacctcgatt ggcgtggtga tgcatgaatc tgcgtcgaaa gcgaagaagg
     9061 cccaggccaa ggccgacggc cagaaaccga cccttacgga gcactatctc gaccagctgc
     9121 agtacctccc cggcgtgcgc gagctcatcg gcgacggagg cgaatttatc cccgggagca
     9181 cgaagagcgc cgcagactac agctactcgg cttccgcgta ttctggcgac cattttcgcg
     9241 tcatcggaga cgcagccaac tttgtggacc cgttcttctc ctccggagtt cacattgcga
     9301 tgaccggcgg gttatctgca gcgataacaa tctgcgcatc catcaggggt gacgccgatg
     9361 agaatattgc acaaaggtgg cacgatgcca aggttggaat tgcgcatacc agattcctgt
     9421 tcgtcgtact cggcgcgtac cagcaaatgc acttgcagtc tgcccctgtc ctgagcgata
     9481 tcaatgcaga aaactttgac gatgcatttg agatgttccg ccccatctac ggcctcgcag
     9541 acagccagac aaggctcaca gatgcaaagg tgcagcacat gatggatgtc tgccaaagct
     9601 tcttcgaccc atatgtcagc gaggagcatg tcctcggtgt acgtgcacgc tatggacctg
     9661 catctgccac gcttgccggc cccgtgctgg gttcggagca gatcgagcag atggtcggtg
     9721 gtgacacccc agagcgtgag atggacacgc gcgtgctgcg caagtttgat gcattgaaag
     9781 tgttcagtga cgatgtcgag gcgacatata tggagcgaac tgtcatttaa ctccttaagt
     9841 tactttaatg atttagtttt tattattaat aattcatgct catgacatct catatacacg
     9901 tttataaaac ttaaatagat tgaaaatgta ttaaagattc ctcagggatt cgattttttt
     9961 ggaagttttt gttttttttt ccttgagatg ctgtagtatt tgggaacaat tatacaatcg
    10021 aaagatatat gcttacattc gaccgtttta gccgtgatca ttatcctata gtaacataac
    10081 ctgaagcata actgacacta ctatcatcaa tacttgtcac atgagaactc tgtgaataat
    10141 taggccactg aaatttgatg cctgaaggac cggcatcacg gattttcgat aaagcactta
    10201 gtatcacact aattggcttt tcgctagtct tatctaaaaa ttgcctttat agtccgtctc
    10261 tccagtcacg gcctgtgtaa ctgattaatc ctgcctttct aatcaccatt ctactgttta
    10321 attaagggat tttgtcttca tcaacggctt ccgcccaaaa aaaagtatga cgttttgccc
    10381 gcaggcgtga agctgcccat cttcacgggc ctgacctcct ctgccggaac accggccatc
    10441 tccaactcat aaattggaga aataagagaa tttcagattt tcagaggatg aaaaaaaaaa
    10501 ggtagagagc ataaaaatgg ggttcacttt ttggcaaagt tacagtatgc ttattacata
    10561 taaatagagt gccgataatg gctttttttc atcttcgaaa tacgcttgct actgctcttc
    10621 cagcgttttt attacttctt tcttgtttct ccttagtata taaaatatca agctacaaca
    10681 agcatacaat caactgtcaa ctgtcaatta tattataata cactatgtct gcgactgcag
    10741 gcatctcaca ggtcgctctg ggaggctcag tgcccccact cactgctcat gctatctctg
    10801 tatctctgcc gacatggaat gacaacgttg ggtatgagaa gggcgagaag agggtcgtgg
    10861 atgcgatggt cactggctac ccgcgtttct tcatccatcg cagtattcaa aagctagctc
    10921 gtatctgcga gcagaagttt ggcgcgaacg gtgaacgatg cctgctgtgt ccgactcgca
    10981 aagttgcaga ggcgtgccgc tcctacatga tcgatagatc tgcgaaggct ggctcacagg
    11041 tcactgtgcg cctcgttcaa ttcttcataa gccccgaggg cgagaaagat tgcaccaacc
    11101 gtagcatcga actccatatt gccctgttcc cgggcgagtc cttcccgttt gccaaagaat
    11161 tttggcagca cacaggtttg tgcatctcga gccgtcgtgc tgagcgctgc ttgtctatgt
    11221 tgccggagga gagtcccgtc tcgggatctc cgacgtcccc atctctcgct cgcccgccca
    11281 ccaagcctgg aaatagacac tactctaaca agagtctcag ctggactccc tcgacatcac
    11341 ctccattgtc acagccgcca ggctccactt ccagatcccc cgcagcgcct ggagagaacc
    11401 tcagtttcga tcagaccact tacgtcgagg aacgctacgg tcgcaactta cccctcgagt
    11461 ccgctgcgtt cacaaaacgt gcactccgcc gccgcatagc aggggtgctc gtccgagaca
    11521 cccctagtga gtggtctact gcaggagcgc aagaggcaga agttggtcca agcacgagag
    11581 gcgtggaaag tgtcacagaa gacgatgtct tcctctaccc gaccggtatg tcggcgatct
    11641 ggagtgctca tcagctggcc cttggtacac gtccaactgc aaagagtatc tgctttggat
    11701 tcccatatac tgatactctc aagattctag agaagtgggg acctggctgt cacttcttgg
    11761 gctatggcct cgattcggac atagacgaac tggaggagat cctcaggcga gaatctgcag
    11821 atacgactca tccgcctgca ttggcgctct tcactgagtt cccttccaac ccgttgctgc
    11881 gctcggcgga tctgccacgt ctccgcgccc tggcagacaa atatgacttc ctcatcgtcg
    11941 ttgacgagac gattggcaac tttgtcaacg tcgaagtgct gccgtacgca gacgttgttg
    12001 tcagcagcct gagcaaaatc ttcagcggcg acgccaatgc tatgggtggc agcctggtgc
    12061 tcaacccaca aggccgccac tacgcagcgc tcaaagctca catggcgcag acgtacgaag
    12121 acgtgtactt tgacgaggac gcgatctaca tggaacgcaa ctcgcgcgat ttccagcagc
    12181 gcatcgaggt cgtcgacggc aatacgctag ccgtctgcac cctcctgcgc gagcgctcca
    12241 tcgtcggggg ctccaccgac gcgcacaccg tcgtcaagga cgtgttctac ccacggtggc
    12301 agaccgccga gcattacgag aagtgccgcc ggccggacgg gggctatggc ggactattct
    12361 cgatcacgtt cacgaccacc gcagcgtctc gcgcgttctt cgacgcactc cgctgccaca
    12421 agggcccgag cctcgggacg aactttacgc tggcgtgccc gtacacgatt ctggcgcact
    12481 tcctggagct ggactgggcg gcgcagtatg gcgtcgagga gggccttgta cgtgtcagcg
    12541 tcggcatgga ggagagacaa gaactgttga agatcttcga ggatgcgctg ctcgcggcag
    12601 aggcgacagt ggaaagccag tgagcgaatt tcttatgatt tatgattttt attattaaat
    12661 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
    12721 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
    12781 aggtcgctct tattgaccac acctctaccg gcatgcctct caaatctttt agcgccaagg
    12841 actccaacta attgtatctt gaatttgcct ttacgatccg tttgtccagt cacggcatgt
    12901 atatcttatt aatcctgcct ttctaatcac gtattctaat gttcaattaa gggattttat
    12961 cttcatcaac ggctcccacg caaaaaatga cgttttgcac acagacacga aatacacctt
    13021 ccaccggaac aacggccatc tccaacttat aagttgggga aataagacaa tttcagactt
    13081 cagagaatga aaaaaaaaaa aggtacatca cagatggggt tcaggtttgc tacaattgca
    13141 gggagcctgt cacatataaa tagacctcca gtgatgatat ctttcagtct tcaaacgtct
    13201 cttgtcacag ttctggtcgt tctatatcac atctctcttg gttctactta ttgtctataa
    13261 tatcaagcta cagcaagcat acaatcaact atctaccata ccataataca caatgaaggc
    13321 tgtggtaaca aacggaaacg gggcatttgg gctgaagtca gtcaaggttc cgacacctgg
    13381 gcggggtgag gttctggtga aggtcatcgc cgcatctcaa aatccaaccg actggaaaac
    13441 ggctatgctt catctcaagc ctggaaacat cttgggctgt gactttgcgg gtgtcatagc
    13501 agatatcggc ccagatgtcg acgcctcctt aatgataacg cgcggcaagc gcgtcgcagg
    13561 agccgttcat ggaggcgtca gcccaaacgg cgcctttgcc gaatacgtga cagtactggc
    13621 ggacatggtc atcgcgatcc cagacaagct ttcattcgag gacgcttcgc aactcggcat
    13681 cgcttgctac acgacatgcc agtgtctcta ccaggaacta catctaccga cgcctctgga
    13741 acccctagaa gtgccgatgg acgttctcat ctggtccggc acgtcgagca caggtcaata
    13801 tgcagtacag tttgccaagc tcgcagggtt gcgagtcctc agcacggcat ctccgaagaa
    13861 ttttgaactt gtaaaagatc taggtgcgga cgaagtcttc gactacgcag actcgaagac
    13921 agcacgaaag atcttcgcgg cgacgagtgg cactctgaaa cacgctgtag attgtatcag
    13981 cgagggcatg accccgaacc aggtcagtat gtcgctgagc aaggaagggg gaacgattgc
    14041 cacgcttttg ccgtacgaaa gccgtaagaa gggtgtagag acacgatttg ttcttgcgta
    14101 caaattcttt ggcaaggaca tcgagttccc tttcgtcttc ccggcggatc aagagcatag
    14161 gaagaatgct cctgtgtatt gtagactcat acaggaagtc atcacgtgct ttgggctgaa
    14221 ggctacgccg gtgcgcatct tcccttacgg cttggcgagc gtctcagatg gatttgagta
    14281 tatgagatcc gggaagcttt ctgcggagaa gatcacatac agaatttcgg ataccccagg
    14341 tctagataag tgggatgtgg ccccgactgg gagtgtcaga ggttcctcgt gaacaggccc
    14401 cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc
    14461 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
    14521 ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
    14581 tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
    14641 ttgggacgct cgaaggcttt aatttgcatt tattgaaaag taaatatctc gtaacccgga
    14701 tgctttgggc ggtcgggttt tgctactcgt catccgatga gaaaaactgt tcccttttgc
    14761 cccaggtttc cattcatccg agcgatcact tatctgactt cgtcactttt tcatttcatc
    14821 cgaaacaatc aaaactgaag ccaatcacca caaaattaac actcaacgtc atctttcact
    14881 accctttaca gaagaaaata tccatagtcc ggactagcat cccagtatgt gactcaatat
    14941 tggtgcaaaa gagaaaagca taagtcagtc caaagtccgc ccttaaccag gcacatcgga
    15001 attcacaaaa cgtttcttta ttatataaag gagctgcttc actggcaaaa ttcttattat
    15061 ttgtcttggc ttgctaattt catcttatcc tttttttctt ttcacaccca aatacctaac
    15121 aattgagaga aaactcttag cataacataa caaaaagtca acgaaaaatg cgggtctggt
    15181 acatcatgca ttacgtctac tcattgccac ccagcgcggt ccacatcagc aacaatctat
    15241 atacagtggg gaaattagac cgtccgtcag tctcgctggg acttgattac tttccaaccc
    15301 gaacctttcc aacccttcgc cgtcggagat ggagcggaat aaagcctggc tgcggggcgc
    15361 tgcgggacat aaaggaggac atggagatat ctctgatcaa gccgaacttg gtacttcgga
    15421 agacacggca cgacatcgcg ttggtaagca tggcgttggg tacggtgaac atcgtgcggt
    15481 atgtgacggg aacgccggtc gcatacacca ttgcgacgga caaagctatc cgactcacac
    15541 aaagtcgagt tgtcaaccct gcaaaagcgt acgagagaac gcaggaagat actcccccgt
    15601 ccactcggga attcatcagg agacgtattg agattctcgt gtacctgcgt catgctggat
    15661 tagcagaaac tgaaatgaag cctctcgcgg ggctgctgca cccatgctat gttcaagagg
    15721 ctcagaggat cattcgatgt gatggcttcg gcaagtcccc atggattacg cagcacaaac
    15781 ttggatgcgt ttcgtctgag gacaaagttg aacgaggaaa cccactgaga accccgctgt
    15841 ttacctctgc gactgatcga ctgcgaaagt gtacatccac gacatgtgaa atgcacaagg
    15901 gcccagactc ttttaagacg cttcgggtcg gctcacggtc ccagtgggca agcgcaatac
    15961 atcgtcaggc cgacgttgtg cgagtatggg tggacagcat cgattctgca ctcgctgcga
    16021 cggagaacct gccagtggag gcgcaggaac tgaatgatgt tcttgcactc aatgaggacg
    16081 agagtatcgg gtgccaggtg gagactcgga gaggatgagg agattgataa gacttttcta
    16141 gttgcatatc ttttatattt aaatcttatc tattagttaa ttttttgtaa tttatcctta
    16201 tatatagtct ggttattcta aaatatcatt tcagtatcta aaaattcccc tcttttttca
    16261 gttatatctt aacaggcgac agtccaaatg ttgatttatc ccagtccgat tcatcagggt
    16321 tgtgaagcat tttgtcaatg gtcgaaatca catcagtaat agtgcctctt acttgcctca
    16381 tagaatttct ttctcttaac gtcaccgttt ggtcttttat agtttcgaaa tctatggtga
    16441 taccaaatgg tgttcccaat tcatcgttac gggcgtattt tttaccaatt gaagtattgg
    16501 aatcgtcaat tttaaagtat atctctcttt tacgtaaagc ctgcgagatc ctcttaagta
    16561 tagcggggaa gccatcgtta ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc
    16621 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
    16681 tggcgagact caaacaagat atattttgcc cgccttacag acgaaactag ttggaggtta
    16741 tggagcatac tatcacgtgg gcggccactg gtgagttact acaccccagg ggcaacgttg
    16801 atgctcctaa aaaactctgg ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt
    16861 ttgcaattca gggcttgatc tacacattac cctgttatcc ctattaatta atacggttat
    16921 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    16981 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    17041 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    17101 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    17161 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    17221 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    17281 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    17341 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    17401 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    17461 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    17521 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    17581 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    17641 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    17701 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    17761 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    17821 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    17881 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    17941 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    18001 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    18061 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    18121 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    18181 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    18241 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    18301 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    18361 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    18421 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    18481 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    18541 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    18601 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    18661 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    18721 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt
    18781 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
    18841 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt
    18901 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
    18961 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa
    19021 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag
    19081 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt
    19141 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca
    19201 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat
    19261 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt
    19321 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc
    19381 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga
    19441 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa
    19501 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt
    19561 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt
    19621 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt
    19681 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
    19741 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
    19801 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
    19861 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
    19921 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
    19981 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
    20041 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
    20101 ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    20161 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctcggcgc gccattaccc
    20221 tgttatccct aatatacaac gcaaagaagc tggaaaattg gcgagggaat cctgtttctg
    20281 tctatccaag aatgggcatg aggtggcaac cgtcgtgcta gcgtacaggg tgcactttgt
    20341 aaccatttgg gacaccggac actcgctgtt ttcgaaatta ccctttaagc gcgggtattg
    20401 aaccaggctt atgcccaaga tcgtagcaag catttaaatg acggtatcga taagcttgat
    20461 atcgaattcc tgcagcccgg gggatccact agttctagag ttaattaa
//
