LOCUS       sequence-217882-       12258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12258)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHTV86.1-2.2
FEATURES             Location/Qualifiers
     source          1..12258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1416..1715
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4432..4652
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4653..5456
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(8405..8425)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8405..8423)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8762..9350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8842..8861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9521..10312,10313..10381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10144..10163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10382..10486)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10513..11855
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11476..11523)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11896..11914
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12233..12249)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12233..12249)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgcacccag aacataaagt cgaacccgct tcttccgccg
      541 tctccacttt gttctacatt ccatacctga tctgggaatt taccgagtcc aatttcccaa
      601 cctttgtcat tccaaacacc actttcggtg tcttgggcgc tttggctggt tctcacttgg
      661 ctgatggtca attaccatct ttgtggcaat tgtcccaagc tttgcccttg gtcgttgcct
      721 tcaattggta caatgtcctg atcttcgatc tggccaatca aagatccgaa gaatccgtca
      781 aagaggacct gattaacaag ccctggaggc caattccatc cggtaaaatt accggcgaac
      841 aaactagaag atccatgttg gttgccgtcc ccatcgcctt ggctttgaat tattggttgg
      901 gtgtctggaa agaaggtgtc ttcatccaaa tcttgacttg gttatacaac gacctgaagg
      961 gcggcgacga agttgtcagg gatttgatca tttccatcgc ttacggttgt ttcaaccacg
     1021 gttccttgag aattgccttg ggttcccaag ccactgtcaa tcaagaaggt atgacctgga
     1081 tcctgatcat tagctctgtc atcttgacca ctatgcaagt tcaggatttg aaggaccaag
     1141 agggcgatag gactagaggt aggctgtcta ttccattgct gttgggcgaa aagttttcca
     1201 ggttctctat cgctatttct gccgctgttt ggtcctgtgt ctgtccattt ttttggaagt
     1261 tgccaccatt ggcctatgct ggtccaatgg ttattggtgc tgtcttgatg acctccgttt
     1321 tgttcaggaa gaccgttaaa gccgacgatc aatcttggaa gatctggtgc ttgtggacta
     1381 tgtccttgta ctctttgcca ctgtttactt tgtgagcgga tctcttatgt ctttacgatt
     1441 tatagttttc attatcaagt atgcctatat tagtatatag catctttaga tgacagtgtt
     1501 cgaagtttca cgaataaaag ataatattct actttttgct cccaccgcgt ttgctagcac
     1561 gagtgaacac catccctcgc ctgtgagttg tacccattcc tctaaactgt agacatggta
     1621 gcttcagcag tgttcgttat gtacggcatc ctccaacaaa cagtcggtta tagtttgtcc
     1681 tgctcctctg aatcgtctcc ctcgatattt ctcattttcc ttcgcatgcc agcattgaaa
     1741 tgatcgaagt tcaatgatga aacggtaatt cttctgtcat ttactcatct catctcatca
     1801 agttatataa ttctatacgg atgtaatttt tcacttttcg tcttgacgtc caccctataa
     1861 tttcaattat tgaaccctca cgatccagtt ctccagtgac acagccttta tctggtcaaa
     1921 cctttctttc taatcaccta tgctgatgct taattaaggg atttttgtct ccatcaacgg
     1981 catgcgccca aaaatgacgt tttttttaac ccatagacac gaaactaccc attttccacc
     2041 ggcctgacct accaccggaa caacggccat ctccaacttg caagttgggg aaattaagag
     2101 catcgcaggt ttaatggaag aaaaaaaaaa ggtacagcac agcgcaaatg gagttagttc
     2161 ccttatgtca cacactcaca cacagtcggt cagatcaagc atactgggtg cgtataaata
     2221 gagtggccat tgccaccctg tttatctcaa aatctgtctt gttagtggtc ttctcccttt
     2281 ttcaggttac aattctcttg tttctactta gtatataagt atatcaagct atattaagca
     2341 tactatcaac tgtcaactct atcctcaaaa tacaatacaa aatggatttt tactacgcca
     2401 atcaacaagc tatcttgact gccgttggtg ctttgtctgg tatcatcgct catattactg
     2461 tctatatccg cggtgaatgg cacgtccaag ccccacaaat taccttgcaa ttgttcgctt
     2521 tgtacgttgg cgttccagtt ttagtcagaa ttttgaagga cactgtcgtt gcccaaatcc
     2581 tgcagggtgt cgtgttttgg tcctacggct atattctggg tttgatcgcc tctattttgt
     2641 tgtataggat tgttttgcat cccctgacta gggcctcgtt tcagggtcca tggtatgcca
     2701 gattcactaa gatttggcat gtttgggctg caagaggttc catgaaccat agggtcttgg
     2761 acgatttgca caaaaagtat ggcaacttcg tcaggactgg tccagctgaa attacagtct
     2821 tccatccaga tgttttcatg gccgtcgatg gtcccagatc tgaatgtatc aaagccgaat
     2881 ggtacgacat tttacatccc ggtcaagcct tggtcactgc tagaaataag tccgttcatg
     2941 ctgctaggag aagggaatgg aattctggct tcactactaa agctttgtcc gaacacgagg
     3001 ctaagatcct gaagtacgtt gacaagttgg atcagtgtat tgccgaagac gcgaagttgg
     3061 aaaatgtcac caatatgcgt gatttgctgt tgtggttcgg cttcgatatc atgggcgact
     3121 tcgtcttctc caagtctttt ggtatgttgg agaacaagca gtggcatcat attattgtca
     3181 gactgcaaag ggctctgtct ctactaggtc cattctcctc cgtcccatgg ttgatccaaa
     3241 ttggttttaa actggctcca agggtgtctg tcttgaaaga ttggttcgac actgttgcct
     3301 ggtgtcaatc ccaaatgagg gatagactgg aaaacggttc tgcttcccaa agccaagact
     3361 tgtcctacta cttgatggaa aaggattctg aaggcaacgg tgctactgag aacgaaaaat
     3421 ggcactgggc tgctggtgat tcattgttgg ctattgtcgc tggttctgaa ccaacagcag
     3481 ttgctttgat tgctgttttt tgtgaactag gtaggcaccc acagcacgct gagcaaattc
     3541 atgaagaggt caagaacgtg gacgttactg atttgaagac tctggtgtct ttgccccatt
     3601 tgaatgccgt catcaaagaa tccctgaggt tgcacccagc tttgttgacc ggtggtaata
     3661 ggaaaactac cgcttttggg ttgaccattg atgatcaatt tatcccaccc aataccacta
     3721 ttgtggcccc caggtacact attggtaggt tggagtcttg tttcgagagg ccatctgatt
     3781 ttatccctga aagatggact actaggcctg aaatggtcag aaatggtgct gcttacgctc
     3841 cattcggtac tggccatcac tcttgtctgg gtaaaacttt ggctctggat actatgaaat
     3901 acgttctggc taggatcttg aaaaactaca ggttcagggc tgctccaggt gaagattcct
     3961 tgtctgccct ggaaaccgtc agggatcagt ttacttctaa cccaggcatt ctgaaattgc
     4021 agtttgaaaa gagacatact atcgactgaa caaatcgctc ttaaatatat acctaaagaa
     4081 cattaaagct atattataag caaagatacg taaattttgc ttatattatt atacacatat
     4141 catatttcta tatttttaag atttggttat ataatgtacg taatgcaaag gaaataaatt
     4201 ttatacatta ttgaacagcg tccaagtaac tacattatgt gcactaatag tttagcgtcg
     4261 tgaagacttt attgtgtcgc gaaaagtaaa aattttaaaa attagagcac cttgaacttg
     4321 cgaaaaaggt tctcatcaac tgtttaaaag gaggatatca ggtcctattt ctgacaaaca
     4381 atatacaaat ttagtttcaa agatgaatca gtgcgcgaag gacataactc attcaattca
     4441 attcatcatt ttttttttat tctttttttt gatttcggtt tctttgaaat ttttttgatt
     4501 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     4561 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     4621 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     4681 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     4741 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     4801 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     4861 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     4921 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     4981 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     5041 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     5101 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     5161 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     5221 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     5281 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     5341 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     5401 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     5461 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     5521 atcagttatt accctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5581 atcaggatag gaaaaaaccg agcttccttt catccggcgc ggctgtgttc tacatatcac
     5641 tgaagctccg ggtattttaa gttatacaag ggaaagatgc cggctagact agcaagtttt
     5701 aggctgctta acattatgga taggcggata aagggcccaa acaggattgt aaagcttaga
     5761 cgcttctggt tggacaatgg tacgtttgtg tattaagtaa ggcttggctg gggatagcaa
     5821 cattgggcag agtatagaag accacaaaaa aaaggtatat aagggcagag aagtctttgt
     5881 aatgtgtgta acttctcttc catgtgtaat cagtatttct acttacttct taaatataca
     5941 gaagtaagac agataaccaa cagcctttcc cagatataca tatatatctt tatttcagct
     6001 taaacaataa ttatatttgt ttaactcaaa aataaaaaaa aaaaaccaaa ctcacgcaac
     6061 taattattcc ataataaaat aacaacatgg cctcttatga ctatattatc gttggtggtg
     6121 gcttggctgg cttagttttg gctgctagat tgactgaaga cagttccaaa agggttttgg
     6181 ttttggaagc cggtgaagat ttgactgctg atgctagaac ttctgtcccc gctatgtggc
     6241 caactttgtt gaatactgac gctgattgga aattcagaac agccgcccag gctggtttca
     6301 acaataggga gatttcattc ccacaaggta aattgttggg cggcagctct gccttgaatg
     6361 gtttgtcctt tactccatcc tccaaagcta acgttgacgc ttgggctaag ttgggtaatc
     6421 caggttggga ttgggcttct ttctctaagt ctttagccaa gtcttatact ctgacttcca
     6481 aggctggtac tactggttct ggtccattgc aattgtccta cccaaacgac accaacaact
     6541 cttggccaaa aatttggcaa caaaccctgg gttccttagg ttttaggcaa tccggtgaat
     6601 cctacacaca acaatcttcc ggtcccgtta ctgttgccga ttctattcat ccagctacta
     6661 aaacccgctc ttacgctggt aatgcctatt tggaaccagc taggtctagg gaaaacttga
     6721 ctgtcgttac tggagctgct gtttctaaaa ttgtcttcgc gaaagatggc tctgctgtta
     6781 ctgctgaagg tgttcagtac gtcaaggatg gcgaaactca aaccgccacc ggtaaagaaa
     6841 tcatcttgac tgcaggcact attaattctc caaggattct agaacactcc ggcgtcggtg
     6901 attctaagtt gctagccaaa ctgggtgtcg atgttgttat tgacaacccc aatgttggtg
     6961 agaatctgca aaaccaccca atggcaggtt tgtctttcga ggccttagaa gatgccgaaa
     7021 ccattgatgg tttatctaga caagatccag cagctttgca agctgctatg gctgcttacg
     7081 ctcaacaatt gggtccattt gcttcttctg gcactaataa cgctgctcaa ctgccattcc
     7141 caggtattca gactgccgag ggtaaagctg aagttgaggc tttgattgaa aagtacatta
     7201 ctggtgctaa gaacgtcact tccccatttg acaaggccca agccgagttc gtcaggtcta
     7261 ttttgacttc tccagaggaa gcttctggtt actacatctc cttttctggc ttcgcttctt
     7321 ttaacccaga tggttctatg gctccaccac ctccaggtac tgaaaaatac ttctctgtcg
     7381 ctttgttgtt gactcatcca ttgtccaggg gctccgtcca cattaactct gcctctgaaa
     7441 cctctactga tgttactatc gacccacagt acttctccca tccactggac attgaaatct
     7501 ttgctagaca cttgcgcttc gttgaatctt tggcctctgc tgaaccattg gcttctcatt
     7561 tgaaggttgg cggtaagaga aacccagctg ctcctccagc tggtattttt tctgctgcat
     7621 ctggtttgga aaaggcgaaa gaatatttga gacagactgc tgttggtgct caccacttca
     7681 ctggtacttg ctccatgatg ccaaaagatt tgggtggcgt tgttgatgct gaattgaggg
     7741 tttatggctg tccaaatttg agggtcgctg atgcctctat tatcccattg actccaagag
     7801 ccaatccaca agctactgtt tacggtgttg ctgaacatgc tgctaccatt attaaaggtg
     7861 gtttgtaagg agattgataa gacttttcta gttgcatatc ttttatattt aaatcttatc
     7921 tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta aaatatcatt
     7981 tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac agtccaaatg
     8041 ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg gtcgaaatca
     8101 catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac gtcaccgttt
     8161 ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat tcatcgttac
     8221 gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat atctctcttt
     8281 tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta ttcgatattg
     8341 tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc tccagctttt
     8401 gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat atattttgcc
     8461 cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg gcggccactg
     8521 gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg ctggacgcaa
     8581 gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc tacacattac
     8641 cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac gcaggaaaga
     8701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9181 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9301 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9361 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9421 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10501 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
    10561 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
    10621 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
    10681 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
    10741 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
    10801 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
    10861 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
    10921 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
    10981 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
    11041 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
    11101 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
    11161 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
    11221 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
    11281 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
    11341 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
    11401 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
    11461 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
    11521 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
    11581 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
    11641 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
    11701 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
    11761 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
    11821 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg
    11881 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    11941 gacggtgaaa acctcggcgc gccattaccc tgttatccct aatatacaac gcaaagaagc
    12001 tggaaaattg gcgagggaat cctgtttctg tctatccaag aatgggcatg aggtggcaac
    12061 cgtcgtgcta gcgtacaggg tgcactttgt aaccatttgg gacaccggac actcgctgtt
    12121 ttcgaaatta ccctttaagc gcgggtattg aaccaggctt atgcccaaga tcgtagcaag
    12181 catttaaatg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact
    12241 agttctagag ttaattaa
//
