LOCUS       sequence-217889-        4856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on 
            C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..4856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..112
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     174..193
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             879..905
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             906..926
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    936..1160
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1400..1419)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1465..1520
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1519..1538)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1881..1897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1881..1897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1894..1916)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1905..1921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1929..1935,1936..1953,1954..1959))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1974..1995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2080..2214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2234..2368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2271..2290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2325..2344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2436..2453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2607..3195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2687..2706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3366..4157,4158..4226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3989..4008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4227..4331)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4362..4741
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
       61 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
      121 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
      181 gctggttatt gtgctgtctc atcattttgg caaagaattg attaattcga gcgaaggcgc
      241 gcccattact cgcatccatt ctcaggctgt ctcgtctcgt ctcgcacgcg taagtctact
      301 ccggagggtc tggctccgga tcaagtggtg gcagcggtac cgcgccaact caagacgagt
      361 tcacccaact ctcccaatca atcgccgagt tccacacgta ccaactcggt aacggccgtt
      421 gctcatctct cctagctcag cgaatccacg cgccgccgga aacagtatgg tccgtggtga
      481 gacgtttcga taggccacag atttacaaac acttcatcaa aagctgtaac gtgagtgaag
      541 atttcgagat gcgagtggga tgcacgcgcg acgtgaacgt gataagtgga ttaccggcga
      601 atacgtctcg agagagatta gatctgttgg acgatgatcg gagagtgact gggtttagta
      661 taaccggtgg tgaacatagg ctgaggaatt ataaatcggt tacgacggtt catagatttg
      721 agaaagaaga agaagaagaa aggatctgga ccgttgtttt ggaatcttat gttgttgatg
      781 taccggaagg taattcggag gaagatacga gattgtttgc tgatacggtt attagattga
      841 atcttcagaa acttgcttcg atcactgaag ctatgaacta cccatacgat gttccagatt
      901 acgctcccaa gaaaaagcgg aaggtgtgag aattcctgtg ccttctagtt gccagccatc
      961 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1021 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1081 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1141 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     1201 tccccacgcg ccctgtagcg tgtaccgctg ggagttcgta gacggaaaca aacgcagaat
     1261 ccaagcgcgg ccgcccagga tacatagatt accacaactc cgagcccttc caccgctagc
     1321 gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     1381 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     1441 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     1501 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     1561 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     1621 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     1681 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     1741 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     1801 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     1861 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1921 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1981 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2041 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2221 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2341 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     2401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4321 ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag
     4381 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     4441 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     4501 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     4561 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     4621 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     4681 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     4741 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     4801 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggg
//
