LOCUS       sequence-217921-        5682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA
FEATURES             Location/Qualifiers
     source          1..5682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1768..1992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2232..2251)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2297..2352
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2351..2370)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2713..2729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2713..2729)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2726..2748)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2737..2753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2761..2767,2768..2785,2786..2791))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2806..2827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2912..3046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3066..3200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3103..3122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3157..3176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3268..3285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3439..4027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3519..3538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4198..4989,4990..5058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4821..4840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5059..5163)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5194..5573
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg ccgccagcga cgaagtgaac ctgatcgaga gcagaaccgt
      721 ggtgcccctg aacacctggg tgctgatctc caacttcaag gtggcctaca acatcctgcg
      781 gaggcccgac ggcaccttca acagacacct ggccgagtac ctggaccgga aagtgaccgc
      841 caacgccaac cctgtggacg gcgtgttcag cttcgacgtg ctgatcgacc ggcggatcaa
      901 cctgctgagc cgggtgtaca gacccgccta cgccgatcag gaacagcccc cctctatcct
      961 ggatctggaa aagcccgtgg atggcgacat cgtgcccgtg atcctgttct tccacggcgg
     1021 cagctttgcc cacagcagcg ccaatagcgc catctacgac accctgtgca gacggctcgt
     1081 gggcctgtgc aaatgcgtgg tggtgtccgt gaactaccgc agagcccccg agaaccctta
     1141 cccctgcgcc tacgatgatg gctggatcgc cctgaactgg gtcaacagca gaagctggct
     1201 gaagtccaag aaagacagca aggtgcacat ctttctggcc ggcgatagca gcggcggcaa
     1261 tatcgcccat aacgtggccc tgagagccgg cgagtctggc atcgatgtgc tgggcaatat
     1321 cctgctgaac cccatgttcg gcggcaacga gcggaccgag agcgagaagt ctctggacgg
     1381 caagtacttc gtgaccgtgc gggaccggga ctggtactgg aaggcctttc tgcccgaggg
     1441 cgaggacaga gagcaccccg cctgcaatcc cttcagcccc agaggcaaaa gcctggaagg
     1501 cgtgtccttc ccaaagtccc tggtggtggt ggccggcctg gacctgatca gagattggca
     1561 gctggcctat gccgagggcc tgaagaaagc cggccaggaa gtgaagctga tgcacctgga
     1621 aaaggccacc gtgggcttct acctgctgcc caacaacaac cacttccaca acgtgatgga
     1681 cgagatcagc gccttcgtga acgccgagtg ctccggaggg tctggctccg gatcaagtgg
     1741 tggcagcggt accaagtcta cgaattcctg tgccttctag ttgccagcca tctgttgttt
     1801 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1861 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1921 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     1981 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     2041 cgccctgtag cgtgtaccgc tgggagttcg tagacggaaa caaacgcaga atccaagcgc
     2101 ggccgcccag gatacataga ttaccacaac tccgagccct tccaccgcta gcgcactcct
     2161 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
     2221 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     2281 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     2341 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     2401 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     2461 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     2521 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     2581 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     2641 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     2701 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     2881 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc cagttccgcc
     2941 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3001 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3061 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3181 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3241 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3841 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4081 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4141 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4201 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4261 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4321 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4381 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4441 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4501 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4561 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4621 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4681 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4741 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4801 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4861 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4921 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4981 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5041 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5161 gcgcacattt ccccaaaaag tgccacctgg gtcgacattg attattgact agttattaat
     5221 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     5281 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     5341 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     5401 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     5461 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     5521 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     5581 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     5641 atttatttat tttttaatta ttttgtgcag cgatgggggc gg
//
