LOCUS       sequence-217924-        4973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FLAG tagged Omomyc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Savino M, Annibali D, Carucci N, Favuzzi E, Cole MD, Evan GI, Soucek
            L, Nasi S
  TITLE     The action mechanism of the Myc inhibitor termed Omomyc may give 
            clues on how to target Myc for cancer therapy.
  JOURNAL   PLoS One. 2011;6(7):e22284. doi: 10.1371/journal.pone.0022284. Epub 
            2011 Jul 21.
  PUBMED    21811581
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7):e22284. doi"; pages: " 
            10.1371/journal.pone.0022284. Epub 2011 Jul 21"
COMMENT     Sequence Label: pCbs FlagOmomyc
FEATURES             Location/Qualifiers
     source          1..4973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     508..527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     761..778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    895..916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(931..936,937..954,955..961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    969..985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     974..996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     993..1009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     993..1009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1028..1048
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1030..1048
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1173..1552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1553..1756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1707..1727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1753..1777
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1868..2440
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             2515..2538
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2823..2839)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2824..2840)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(2863..2881)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2864..2881)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2901..2918)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2907..3131
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3188..3207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3189..3207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3217..3234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3217..3233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3226..3248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3375..3830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3462..3481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3672..3693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3856..3960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3961..4029,4030..4821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4179..4198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
       61 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      901 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      961 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     1021 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg
     1081 ccgctctaga gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta
     1141 tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata
     1201 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     1261 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     1321 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1381 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1441 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1501 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     1561 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     1621 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1681 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1741 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1801 ttttgacctc catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa
     1861 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
     1921 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
     1981 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
     2041 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
     2101 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
     2161 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
     2221 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
     2281 aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg
     2341 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
     2401 ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt
     2461 tgttgtgctg tctcatcatt ttggcaaaga attcctcgac ggatccccac catggactac
     2521 aaggacgatg atgacaagcc cgggaccgag aatgtcaaga ggcgaacaca caacgtcttg
     2581 gagcgccaga ggaggaacga gctaaaacgg agcttttttg ccctgcgtga ccagatcccg
     2641 gagttggaaa acaatgaaaa ggcccccaag gtagttatcc ttaaaaaagc cacagcatac
     2701 atcctgtccg tccaagcaga gacgcaaaag ctcatttctg aaatcgactt gttgcggaaa
     2761 caaaacgaac agttgaaaca caaacttgaa cagctacgga actcttgtgc gtaaaagctt
     2821 atcgataccg tcgacctcga gcatgcatct agagggccct attctatagt gtcacctaaa
     2881 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     2941 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3001 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3061 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3121 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgagggggg gcccggtacc
     3181 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3361 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3781 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     3841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4201 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4501 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt aga
//
