LOCUS       sequence-217925-       12508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for constitutive expression of BE4RA in mammalian cells 
            (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE4RA-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..94
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             101..121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             146..832
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(881..904,905..907,908..4981)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             881..4981
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4994..5242
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5255..5275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5276..5524
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5537..5557
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5573..5629
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5630..6229
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5630..5649)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6126..6146
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6245..6833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6298..6318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6716..6727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6905..7138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7216..7337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7253..7272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7307..7326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7377..7512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7532..7551)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7533..7551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7561..7578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7561..7577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7570..7592)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7719..8174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7806..7825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8016..8037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8200..8304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8305..8373,8374..9165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8523..8542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9336..9924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9825..9844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10078..10095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10212..10233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10248..10253,10254..10271,10272..10278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10286..10302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10291..10313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10310..10326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10310..10326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10345..10365
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10347..10365
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10393..10619
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10620..10800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10847..10972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11465..11698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11883..11927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12111..12228
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        12297..12508
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gtgtcgtgac gcgggatccg ccaccatgga ctataaggac cacgacggag actacaagga
       61 tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga agcggaaggt
      121 cggtatccac ggagtcccag cagccatgag ctcagagact ggcccagtgg ctgtggaccc
      181 cacattgaga cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct
      241 ccgcaaggag acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg
      301 acatacatca cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac
      361 agaaagatat ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc
      421 atgcggcgaa tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct
      481 gtttatttac atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg
      541 ggatttgatc tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg
      601 ctggagaaac tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca
      661 tctgtgggta cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg
      721 tctcaacatt ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc
      781 ttgtcattac cagcgactgc ccccacacat tctctgggcc accgggttga aaagcggcag
      841 cgagactccc gggacctcag agtccgccac acccgaaagt gacaagaagt acagcatcgg
      901 cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
      961 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     1021 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     1081 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     1141 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     1201 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     1261 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     1321 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     1381 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     1441 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     1501 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     1561 gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga gcctgggcct
     1621 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     1681 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     1741 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     1801 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     1861 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     1921 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     1981 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     2041 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     2101 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     2161 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     2221 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     2281 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     2341 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     2401 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     2461 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     2521 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     2581 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     2641 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     2701 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     2761 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     2821 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     2881 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     2941 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     3001 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     3061 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     3121 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     3181 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     3241 caaagagctg ggcagccaga tcctgaaaga acacccagtg gaaaacaccc agctgcagaa
     3301 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     3361 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     3421 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     3481 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     3541 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     3601 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     3661 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     3721 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     3781 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     3841 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     3901 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     3961 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     4021 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     4081 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     4141 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     4201 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     4261 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     4321 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     4381 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     4441 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     4501 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     4561 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     4621 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     4681 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     4741 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     4801 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     4861 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     4921 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     4981 ttcaggcgga tctactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt
     5041 tatccaggaa tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc
     5101 ggaaagcgat atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct
     5161 tctgactagc gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg
     5221 tgagaacaag attaagatgc tctctggtgg ttctcccaag aagaagagga aagtcacaaa
     5281 tctctctgac atcatagaga aggagacagg gaaacaactc gtaatacaag agtccattct
     5341 tatgctccct gaggaggtgg aagaagttat cggcaacaaa ccagagagtg acattctggt
     5401 ccataccgcc tacgatgaaa gcacagacga gaacgttatg ttgctcactt ctgacgctcc
     5461 agaatacaaa ccttgggcac tcgtcattca ggacagcaac ggcgagaaca agatcaaaat
     5521 gcttagcggg ggcagcccca aaaaaaagag gaaggtcgct agcggcagcg gcgccaccaa
     5581 cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tgaccgagta
     5641 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5701 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5761 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5821 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5881 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5941 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6001 cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6061 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6121 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6181 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt
     6241 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6301 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6361 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6421 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6481 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6541 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6601 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6661 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6721 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6781 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     6841 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6901 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6961 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7021 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7081 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7141 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7201 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7261 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7321 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     7381 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7441 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7501 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     7561 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7621 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7681 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     7741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8101 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8161 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8221 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8281 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8341 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8401 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8461 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8521 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8581 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8641 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8701 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8761 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8821 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8881 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8941 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9001 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9061 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9121 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9181 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9241 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9301 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9361 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9421 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9481 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9541 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9601 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9661 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9721 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9781 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9841 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9901 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9961 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10021 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10081 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10141 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    10201 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10261 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10321 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
    10381 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
    10441 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
    10501 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
    10561 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
    10621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10801 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
    10861 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    10921 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    10981 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    11041 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    11101 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    11161 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    11221 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    11281 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
    11341 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    11401 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    11461 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    11521 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    11581 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    11641 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    11701 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    11761 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
    11821 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    11881 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
    11941 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
    12001 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
    12061 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagaccacaa ttttaaaaga
    12121 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
    12181 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
    12241 agggacagca gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc
    12301 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
    12361 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
    12421 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
    12481 tcgcaacggg tttgccgcca gaacacag
//
