LOCUS       sequence-217927-       10653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient/stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10653)
  TITLE     Proszynski unpublished
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FFTTH--GFP-Amotl2
FEATURES             Location/Qualifiers
     source          1..10653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             557..580
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             590..613
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             623..643
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             653..673
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             683..709
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(716..718,719..721,722..1432)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(716..718,719..721,722..1432)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(761..782)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1022..1041)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1369..1390
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3902..4023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3939..3958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3993..4012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7214..7316
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7405..7422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7576..8164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7656..7675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8996..9790
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8996..9790
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9050..9069)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9660..9679
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(9838..10218)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9900..9921)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10112..10131
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   10256..10358
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     10446..10596
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc
      541 tggtaccgtc gacatggatt acaaggatga cgacgataag tcaggtggag attacaagga
      601 tgacgacgat aagagcggcc gcgaaaatct gtactttcaa ggcgcatcag gagaaaatct
      661 gtactttcaa ggcggatctg gataccccta cgacgtcccc gactacgcca agcttatggt
      721 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      781 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      841 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      901 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      961 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1021 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1081 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1141 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1201 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1261 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1321 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1381 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtactcaga
     1441 tctcgagatg aggacactgg aagactcctc agggacagtc ctgcaccgtc tcatccaaga
     1501 gcagctgcgc tacggcaacc tgacagagac tcgcactctg ctggctatcc agcagcaggc
     1561 cctgcggggt ggggctggag ctgggggcac ggggagcccc caggcttcct tggagatcgg
     1621 agcacccgag gacagtcagg tgctgcagca agccaccagg caggggcccc agggccagga
     1681 gcatcagggt ggagagaccc acctggcaga gaacaggctg taccggctgt gcccacagcc
     1741 cagcaaagga gaagagttgc ccacctatga ggaggccaaa gcccattcgc agtactacgc
     1801 agcgcagcag gcagggtccc ggccgcatgt tggggaccgg gatcctagag gaggggtgtc
     1861 cggaggcggc cggcgacagg atgaagctct tcgagagctg aggcatggcc atgtgcgctc
     1921 cttgagtgaa cggcttctgc aactgtccct ggaaagaaac ggtgctcggg tccccagcca
     1981 catgagctct tcccacagct tccctcagct ggcccgcagc cagcagggcc cccaaccccg
     2041 agggccccca gctgagggcc cagagccccg cgggccacca cctcagtacc cacacgctgt
     2101 aatggctcag gagactgcgg ctgtcactga cccaagatac cgaccccgaa gcagcccaca
     2161 cttccagcat gccgaagtca ggatcctgca ggcccaggta ccaccggtgt tcctccagca
     2221 gcagcagtac cagtacctgc cgcagcccca ggagcactct ccacccctcc acccggcagc
     2281 tctgggccat ggacccccaa gctcctttgg tccacctgca gtggagggac cacccagtgc
     2341 ccaggccacc ttgggcagtg cccacctggc ccagatggag actgtactga gggagaatgc
     2401 caggctgcag agggacaatg agcgattgca gagagagctg gagagcactt cagagaaggc
     2461 tggccgcata gaaaagctgg aaaatgaaat ccagcggctc tctgaggccc acgagagcct
     2521 gatgaggacc tcttccaagc gtgaggccct ggagaagacc atgaggaaca agatggacgg
     2581 tgagatgaga cggttgcagg acttcaaccg agaccttaga gagagattgg aatcggcaaa
     2641 ccgccacctg gcaagcaaga cccaggaagc ccaggcgggc agtcaggaca tggtggcgaa
     2701 actgcttgcc cagagctatg agcagcaaca ggaacaggag aagctggagc gggagatggc
     2761 actgctgcgt ggtgccatcg aggaccagcg gcgacatgct gaactgctgg agcaggctct
     2821 gggcaatgca caaagccgtg ccgcccgggc tgaagaggag ctacgcaaaa agcaggccta
     2881 tgtggagaag gtggagcggc tgcagcaggc actggggcag ttgcaggctg cctgtgaaaa
     2941 gcgagagcag ttggagctgc gtctgcgcac gcgcctggag caggaactca aagccttgcg
     3001 tgcacagcag aggcagacag gcaccctcgc aggtggtggc ggcagccatg gtgggtccgc
     3061 cgagctcagt gccctgcggc tgtctgaaca gctgcgggag aaggaggaac agatcctggc
     3121 tctagaggcg gacatgacca agtgggaaca gaagtatttg gaagaacggg ctatgaggca
     3181 gttcgccatg gacgcggctg ccactgcggc tgcccagcgc gataccactc tcatccggca
     3241 ctccccccag ccctcgccca gcagcagttt caacgagggc ctgctgccag gcaaccacag
     3301 gcaccaggag atggagagca gattgaaggt gctccatgct cagatcctag agaaggatgc
     3361 ggtgatcaag gtccttcagc agcgctccag gaaagaccct ggcaaggcca cccagggcac
     3421 cctacggcct gccaagtcgg tgccgtccat cttcgcggct gcagtgggaa ctcagggctg
     3481 gcaagggctc gtatccagtg agcggcaaac tgatgcacgg ccagcgggag accgggtccc
     3541 agcagaggag cctccggcca cagctcctct ccctgcccac accaaacatg gcagcagaga
     3601 cgggagcacc cagacggatg gccctgcaga caacacctct gcctgcttgg cctcagaacc
     3661 cgatggcctc ctggggtgca acagtagcca gaggacaccc tctctggact ctatagctgc
     3721 aaccagagtc caggatctgt cagacatggt agaaatactg atctgagata tccgatccac
     3781 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3841 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3901 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4021 ttaacgcgga tctgggcgtg gttaagggtg ggaaagaata tataaggtgg gggtcttatg
     4081 tagttttgta tctgttttgc agcagccgcc gccgccatga gcaccaactc gtttgatgga
     4141 agcattgtga gctcatattt gacaacgcgc atgcccccat gggccggggt gcgtcagaat
     4201 gtgatgggct ccagcattga tggtcgcccc gtcctgcccg caaactctac taccttgacc
     4261 tacgagaccg tgtctggaac gccgttggag actgcagcct ccgccgccgc ttcagccgct
     4321 gcagccaccg cccgcgggat tgtgactgac tttgctttcc tgagcccgct tgcaagcagt
     4381 gcagcttccc gttcatccgc ccgcgatgac aagttgacgg ctcttttggc acaattggat
     4441 tctttgaccc gggaacttaa tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt
     4501 tctgccctga aggcttcctc ccctcccaat gcggtttaaa acataaataa aaaaccagac
     4561 tctgtttgga tttggatcaa gcaagtgtct tgctgtcttt atttaggggt tttgcgcgcg
     4621 cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg
     4681 tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg
     4741 tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag
     4801 gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg
     4861 cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg
     4921 agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga
     4981 ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt
     5041 agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc
     5101 atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt
     5161 ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca
     5221 aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag
     5281 ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc
     5341 tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg
     5401 ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggc
     5461 tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg
     5521 ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg
     5581 cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc
     5641 gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc
     5701 tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc
     5761 tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca
     5821 gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg
     5881 ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg
     5941 cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct
     6001 tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg
     6061 cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc
     6121 cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca
     6181 ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac
     6241 gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggg agtttaaacg
     6301 aattcaatag cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca
     6361 ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag
     6421 gtaagctccg gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt
     6481 ttctgcataa acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta
     6541 caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt
     6601 aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag
     6661 tcataatgta agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc
     6721 gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca
     6781 taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct
     6841 gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag
     6901 ccataacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac
     6961 ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag
     7021 gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa
     7081 cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt
     7141 tcccacgtta cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac
     7201 tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc
     7261 caccccctca ttatcatatt ggcttcaatc caaaataagg tatattattg atgatgttaa
     7321 ttaacatgca tggatccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7381 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7981 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8641 tgccattgct gcagccatga gattatcaaa aaggatcttc acctagatcc ttttcacgta
     8701 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     8761 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     8821 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     8881 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     8941 ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat
     9001 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     9061 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     9121 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     9181 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     9241 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     9301 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     9361 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     9421 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     9481 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     9541 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     9601 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     9661 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     9721 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     9781 gttcttctga attttgttaa aatttttgtt aaatcagctc attttttaac caataggccg
     9841 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     9901 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     9961 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    10021 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    10081 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    10141 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    10201 cgccgctaca gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca
    10261 tcaataatat accttatttt ggattgaagc caatatgata atgagggggt ggagtttgtg
    10321 acgtggcgcg gggcgtggga acggggcggg tgacgtagta gtgtggcgga agtgtgatgt
    10381 tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca aaagtgacgt ttttggtgtg
    10441 cgccggtgta cacaggaagt gacaattttc gcgcggtttt aggcggatgt tgtagtaaat
    10501 ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa aactgaataa gaggaagtga
    10561 aatctgaata attttgtgtt actcatagcg cgtaatactg taatagtaat caattacggg
    10621 gtcattagtt catagcccat atatggagtt ccg
//
