LOCUS       sequence-217933-       10401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient/stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10401)
  TITLE     Proszynski unpublished
REFERENCE   2  (bases 1 to 10401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TAP-Amotl2
FEATURES             Location/Qualifiers
     source          1..10401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        94..297
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     248..268
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             403..576
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             580..753
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             796..816
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             829..849
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             865..888
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             901..924
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             931..948
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     polyA_signal    3439..3560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3476..3495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3530..3549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6751..6853
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(6942..6959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7113..7701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7193..7212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8533..9327
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8533..9327
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8587..8606)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9197..9216
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(9375..9755)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9437..9458)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9649..9668
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   9793..9895
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     9983..10133
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        join(10191..10401,1..93)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
       61 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      121 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      181 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      241 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
      301 ttagtgaacc gtcagatccg ctagagatct ggtaccgtcg acgcggccgc tcgagcctaa
      361 gcttctagat aagatcggga ccgatccagc ctccggacca tggtggacaa caaattcaac
      421 aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa
      481 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca
      541 gaagctaaaa agctaaatga tgctcaggcg ccgaaaggtg tagacaacaa attcaacaaa
      601 gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga agaacaacga
      661 aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
      721 gctaaaaagc taaatggtgc tcaggcgccg aaagtagacg cgaactccgc ggggaagtca
      781 actggtacta gtggagagaa tttgtatttt cagggtcgag gaggtagcga gaatttgtat
      841 tttcagggtg agggtacctc ggcctggagc cacccgcagt tcgagaaagg cggtagcgga
      901 tggagccacc cgcagttcga gaaaggagga tgctgtccag gttgttgcac cggtgattcg
      961 acgcggccgc tcgagcctaa gcttatgagg acactggaag actcctcagg gacagtcctg
     1021 caccgtctca tccaagagca gctgcgctac ggcaacctga cagagactcg cactctgctg
     1081 gctatccagc agcaggccct gcggggtggg gctggagctg ggggcacggg gagcccccag
     1141 gcttccttgg agatcggagc acccgaggac agtcaggtgc tgcagcaagc caccaggcag
     1201 gggccccagg gccaggagca tcagggtgga gagacccacc tggcagagaa caggctgtac
     1261 cggctgtgcc cacagcccag caaaggagaa gagttgccca cctatgagga ggccaaagcc
     1321 cattcgcagt actacgcagc gcagcaggca gggtcccggc cgcatgttgg ggaccgggat
     1381 cctagaggag gggtgtccgg aggcggccgg cgacaggatg aagctcttcg agagctgagg
     1441 catggccatg tgcgctcctt gagtgaacgg cttctgcaac tgtccctgga aagaaacggt
     1501 gctcgggtcc ccagccacat gagctcttcc cacagcttcc ctcagctggc ccgcagccag
     1561 cagggccccc aaccccgagg gcccccagct gagggcccag agccccgcgg gccaccacct
     1621 cagtacccac acgctgtaat ggctcaggag actgcggctg tcactgaccc aagataccga
     1681 ccccgaagca gcccacactt ccagcatgcc gaagtcagga tcctgcaggc ccaggtacca
     1741 ccggtgttcc tccagcagca gcagtaccag tacctgccgc agccccagga gcactctcca
     1801 cccctccacc cggcagctct gggccatgga cccccaagct cctttggtcc acctgcagtg
     1861 gagggaccac ccagtgccca ggccaccttg ggcagtgccc acctggccca gatggagact
     1921 gtactgaggg agaatgccag gctgcagagg gacaatgagc gattgcagag agagctggag
     1981 agcacttcag agaaggctgg ccgcatagaa aagctggaaa atgaaatcca gcggctctct
     2041 gaggcccacg agagcctgat gaggacctct tccaagcgtg aggccctgga gaagaccatg
     2101 aggaacaaga tggacggtga gatgagacgg ttgcaggact tcaaccgaga ccttagagag
     2161 agattggaat cggcaaaccg ccacctggca agcaagaccc aggaagccca ggcgggcagt
     2221 caggacatgg tggcgaaact gcttgcccag agctatgagc agcaacagga acaggagaag
     2281 ctggagcggg agatggcact gctgcgtggt gccatcgagg accagcggcg acatgctgaa
     2341 ctgctggagc aggctctggg caatgcacaa agccgtgccg cccgggctga agaggagcta
     2401 cgcaaaaagc aggcctatgt ggagaaggtg gagcggctgc agcaggcact ggggcagttg
     2461 caggctgcct gtgaaaagcg agagcagttg gagctgcgtc tgcgcacgcg cctggagcag
     2521 gaactcaaag ccttgcgtgc acagcagagg cagacaggca ccctcgcagg tggtggcggc
     2581 agccatggtg ggtccgccga gctcagtgcc ctgcggctgt ctgaacagct gcgggagaag
     2641 gaggaacaga tcctggctct agaggcggac atgaccaagt gggaacagaa gtatttggaa
     2701 gaacgggcta tgaggcagtt cgccatggac gcggctgcca ctgcggctgc ccagcgcgat
     2761 accactctca tccggcactc cccccagccc tcgcccagca gcagtttcaa cgagggcctg
     2821 ctgccaggca accacaggca ccaggagatg gagagcagat tgaaggtgct ccatgctcag
     2881 atcctagaga aggatgcggt gatcaaggtc cttcagcagc gctccaggaa agaccctggc
     2941 aaggccaccc agggcaccct acggcctgcc aagtcggtgc cgtccatctt cgcggctgca
     3001 gtgggaactc agggctggca agggctcgta tccagtgagc ggcaaactga tgcacggcca
     3061 gcgggagacc gggtcccagc agaggagcct ccggccacag ctcctctccc tgcccacacc
     3121 aaacatggca gcagagacgg gagcacccag acggatggcc ctgcagacaa cacctctgcc
     3181 tgcttggcct cagaacccga tggcctcctg gggtgcaaca gtagccagag gacaccctct
     3241 ctggactcta tagctgcaac cagagtccag gatctgtcag acatggtaga aatactgatc
     3301 tgagatatcc gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     3361 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3421 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3481 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3541 aaactcatca atgtatctta acgcggatct gggcgtggtt aagggtggga aagaatatat
     3601 aaggtggggg tcttatgtag ttttgtatct gttttgcagc agccgccgcc gccatgagca
     3661 ccaactcgtt tgatggaagc attgtgagct catatttgac aacgcgcatg cccccatggg
     3721 ccggggtgcg tcagaatgtg atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa
     3781 actctactac cttgacctac gagaccgtgt ctggaacgcc gttggagact gcagcctccg
     3841 ccgccgcttc agccgctgca gccaccgccc gcgggattgt gactgacttt gctttcctga
     3901 gcccgcttgc aagcagtgca gcttcccgtt catccgcccg cgatgacaag ttgacggctc
     3961 ttttggcaca attggattct ttgacccggg aacttaatgt cgtttctcag cagctgttgg
     4021 atctgcgcca gcaggtttct gccctgaagg cttcctcccc tcccaatgcg gtttaaaaca
     4081 taaataaaaa accagactct gtttggattt ggatcaagca agtgtcttgc tgtctttatt
     4141 taggggtttt gcgcgcgcgg taggcccggg accagcggtc tcggtcgttg agggtcctgt
     4201 gtattttttc caggacgtgg taaaggtgac tctggatgtt cagatacatg ggcataagcc
     4261 cgtctctggg gtggaggtag caccactgca gagcttcatg ctgcggggtg gtgttgtaga
     4321 tgatccagtc gtagcaggag cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc
     4381 tgattgccag gggcaggccc ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt
     4441 gcatacgtgg ggatatgaga tgcatcttgg actgtatttt taggttggct atgttcccag
     4501 ccatatccct ccggggattc atgttgtgca gaaccaccag cacagtgtat ccggtgcact
     4561 tgggaaattt gtcatgtagc ttagaaggaa atgcgtggaa gaacttggag acgcccttgt
     4621 gacctccaag attttccatg cattcgtcca taatgatggc aatgggccca cgggcggcgg
     4681 cctgggcgaa gatatttctg ggatcactaa cgtcatagtt gtgttccagg atgagatcgt
     4741 cataggccat ttttacaaag cgcgggcgga gggtgccaga ctgcggtata atggttccat
     4801 ccggcccagg ggcgtagtta ccctcacaga tttgcatttc ccacgctttg agttcagatg
     4861 gggggatcat gtctacctgc ggggcgatga agaaaacggt ttccggggta ggggagatca
     4921 gctgggaaga aagcaggttc ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa
     4981 tcacacctat taccggctgc aactggtagt taagagagct gcagctgccg tcatccctga
     5041 gcaggggggc cacttcgtta agcatgtccc tgactcgcat gttttccctg accaaatccg
     5101 ccagaaggcg ctcgccgccc agcgatagca gttcttgcaa ggaagcaaag tttttcaacg
     5161 gtttgagacc gtccgccgta ggcatgcttt tgagcgtttg accaagcagt tccaggcggt
     5221 cccacagctc ggtcacctgc tctacggcat ctcgatccag catatctcct cgtttcgcgg
     5281 gttggggcgg ctttcgctgt acggcagtag tcggtgctcg tccagacggg ccagggtcat
     5341 gtctttccac gggcgcaggg tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc
     5401 tccgggctgc gcgctggcca gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg
     5461 ccggtcttcg ccctgcgcgt cggccaggta gcatttgacc atggtgtcat agtccagccc
     5521 ctccgcggcg tggcccttgg cgcgcagctt gcccttggag gaggcgccgc acgaggggca
     5581 gtgcagactt ttgagggcgt agagcttggg cgcgagaaat accgattccg gggagtaggc
     5641 atccgcgccg caggccccgc agacggtctc gcattccacg agccaggtga gctctggccg
     5701 ttcggggtca aaaaccaggt ttcccccatg ctttttgatg cgtttcttac ctctggtttc
     5761 catgagccgg tgtccacgct cggtgacgaa aaggctgtcc gtgtccccgt atacagactt
     5821 gagagggagt ttaaacgaat tcaatagctt gttgcatggg cggcgatata aaatgcaagg
     5881 tgctgctcaa aaaatcaggc aaagcctcgc gcaaaaaaga aagcacatcg tagtcatgct
     5941 catgcagata aaggcaggta agctccggaa ccaccacaga aaaagacacc atttttctct
     6001 caaacatgtc tgcgggtttc tgcataaaca caaaataaaa taacaaaaaa acatttaaac
     6061 attagaagcc tgtcttacaa caggaaaaac aacccttata agcataagac ggactacggc
     6121 catgccggcg tgaccgtaaa aaaactggtc accgtgatta aaaagcacca ccgacagctc
     6181 ctcggtcatg tccggagtca taatgtaaga ctcggtaaac acatcaggtt gattcacatc
     6241 ggtcagtgct aaaaagcgac cgaaatagcc cgggggaata catacccgca ggcgtagaga
     6301 caacattaca gcccccatag gaggtataac aaaattaata ggagagaaaa acacataaac
     6361 acctgaaaaa ccctcctgcc taggcaaaat agcaccctcc cgctccagaa caacatacag
     6421 cgcttccaca gcggcagcca taacagtcag ccttaccagt aaaaaagaaa acctattaaa
     6481 aaaacaccac tcgacacggc accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc
     6541 agagcgagta tatataggac taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc
     6601 agaaaaccgc acgcgaacct acgcccagaa acgaaagcca aaaaacccac aacttcctca
     6661 aatcgtcact tccgttttcc cacgttacgt cacttcccat tttaagaaaa ctacaattcc
     6721 caacacatac aagttactcc gccctaaaac ctacgtcacc cgccccgttc ccacgccccg
     6781 cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat
     6841 attattgatg atgttaatta acatgcatgg atccatatgc ggtgtgaaat accgcacaga
     6901 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     6961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     7561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7981 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8161 agtttgcgca acgttgttgc cattgctgca gccatgagat tatcaaaaag gatcttcacc
     8221 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     8281 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     8341 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     8401 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     8461 ttcttgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
     8521 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     8581 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     8641 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     8701 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8761 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8821 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8881 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8941 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     9001 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     9061 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     9121 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     9181 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     9241 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     9301 tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt
     9361 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     9421 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     9481 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     9541 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     9601 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     9661 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9721 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gatcgaatta
     9781 attcttaatt aacatcatca ataatatacc ttattttgga ttgaagccaa tatgataatg
     9841 agggggtgga gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga cgtagtagtg
     9901 tggcggaagt gtgatgttgc aagtgtggcg gaacacatgt aagcgacgga tgtggcaaaa
     9961 gtgacgtttt tggtgtgcgc cggtgtacac aggaagtgac aattttcgcg cggttttagg
    10021 cggatgttgt agtaaatttg ggcgtaaccg agtaagattt ggccattttc gcgggaaaac
    10081 tgaataagag gaagtgaaat ctgaataatt ttgtgttact catagcgcgt aatactgtaa
    10141 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
    10201 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
    10261 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
    10321 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
    10381 cctattgacg tcaatgacgg t
//
