LOCUS       sequence-217938-       11133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient/stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11133)
  TITLE     Proszynski unpublished
REFERENCE   2  (bases 1 to 11133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TAP-GFP-Amotl2
FEATURES             Location/Qualifiers
     source          1..11133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             614..787
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             791..964
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             1007..1027
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1040..1060
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1076..1099
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1112..1135
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1142..1159
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             join(1196..1198,1199..1201,1202..1912)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1196..1198,1199..1201,1202..1912)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1241..1262)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1502..1521)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1849..1870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4382..4503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4419..4438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4473..4492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7694..7796
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7885..7902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8056..8644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8136..8155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9476..10270
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9476..10270
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9530..9549)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10140..10159
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(10318..10698)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10380..10401)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10592..10611
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   10736..10838
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     10926..11076
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc
      541 tggtaccgtc gacgcggccg ctcgagccta agcttctaga taagatcggg accgatccag
      601 cctccggacc atggtggaca acaaattcaa caaagaacaa caaaacgcgt tctatgagat
      661 cttacattta cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga
      721 tgacccaagc caaagcgcta accttttagc agaagctaaa aagctaaatg atgctcaggc
      781 gccgaaaggt gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
      841 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
      901 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatggtg ctcaggcgcc
      961 gaaagtagac gcgaactccg cggggaagtc aactggtact agtggagaga atttgtattt
     1021 tcagggtcga ggaggtagcg agaatttgta ttttcagggt gagggtacct cggcctggag
     1081 ccacccgcag ttcgagaaag gcggtagcgg atggagccac ccgcagttcg agaaaggagg
     1141 atgctgtcca ggttgttgca ccggtgattc gacgcggccg ctcgagccta agcttatggt
     1201 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1261 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1321 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1381 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1441 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1501 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1561 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1621 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1681 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1741 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1801 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1861 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtactcaga
     1921 tctcgagatg aggacactgg aagactcctc agggacagtc ctgcaccgtc tcatccaaga
     1981 gcagctgcgc tacggcaacc tgacagagac tcgcactctg ctggctatcc agcagcaggc
     2041 cctgcggggt ggggctggag ctgggggcac ggggagcccc caggcttcct tggagatcgg
     2101 agcacccgag gacagtcagg tgctgcagca agccaccagg caggggcccc agggccagga
     2161 gcatcagggt ggagagaccc acctggcaga gaacaggctg taccggctgt gcccacagcc
     2221 cagcaaagga gaagagttgc ccacctatga ggaggccaaa gcccattcgc agtactacgc
     2281 agcgcagcag gcagggtccc ggccgcatgt tggggaccgg gatcctagag gaggggtgtc
     2341 cggaggcggc cggcgacagg atgaagctct tcgagagctg aggcatggcc atgtgcgctc
     2401 cttgagtgaa cggcttctgc aactgtccct ggaaagaaac ggtgctcggg tccccagcca
     2461 catgagctct tcccacagct tccctcagct ggcccgcagc cagcagggcc cccaaccccg
     2521 agggccccca gctgagggcc cagagccccg cgggccacca cctcagtacc cacacgctgt
     2581 aatggctcag gagactgcgg ctgtcactga cccaagatac cgaccccgaa gcagcccaca
     2641 cttccagcat gccgaagtca ggatcctgca ggcccaggta ccaccggtgt tcctccagca
     2701 gcagcagtac cagtacctgc cgcagcccca ggagcactct ccacccctcc acccggcagc
     2761 tctgggccat ggacccccaa gctcctttgg tccacctgca gtggagggac cacccagtgc
     2821 ccaggccacc ttgggcagtg cccacctggc ccagatggag actgtactga gggagaatgc
     2881 caggctgcag agggacaatg agcgattgca gagagagctg gagagcactt cagagaaggc
     2941 tggccgcata gaaaagctgg aaaatgaaat ccagcggctc tctgaggccc acgagagcct
     3001 gatgaggacc tcttccaagc gtgaggccct ggagaagacc atgaggaaca agatggacgg
     3061 tgagatgaga cggttgcagg acttcaaccg agaccttaga gagagattgg aatcggcaaa
     3121 ccgccacctg gcaagcaaga cccaggaagc ccaggcgggc agtcaggaca tggtggcgaa
     3181 actgcttgcc cagagctatg agcagcaaca ggaacaggag aagctggagc gggagatggc
     3241 actgctgcgt ggtgccatcg aggaccagcg gcgacatgct gaactgctgg agcaggctct
     3301 gggcaatgca caaagccgtg ccgcccgggc tgaagaggag ctacgcaaaa agcaggccta
     3361 tgtggagaag gtggagcggc tgcagcaggc actggggcag ttgcaggctg cctgtgaaaa
     3421 gcgagagcag ttggagctgc gtctgcgcac gcgcctggag caggaactca aagccttgcg
     3481 tgcacagcag aggcagacag gcaccctcgc aggtggtggc ggcagccatg gtgggtccgc
     3541 cgagctcagt gccctgcggc tgtctgaaca gctgcgggag aaggaggaac agatcctggc
     3601 tctagaggcg gacatgacca agtgggaaca gaagtatttg gaagaacggg ctatgaggca
     3661 gttcgccatg gacgcggctg ccactgcggc tgcccagcgc gataccactc tcatccggca
     3721 ctccccccag ccctcgccca gcagcagttt caacgagggc ctgctgccag gcaaccacag
     3781 gcaccaggag atggagagca gattgaaggt gctccatgct cagatcctag agaaggatgc
     3841 ggtgatcaag gtccttcagc agcgctccag gaaagaccct ggcaaggcca cccagggcac
     3901 cctacggcct gccaagtcgg tgccgtccat cttcgcggct gcagtgggaa ctcagggctg
     3961 gcaagggctc gtatccagtg agcggcaaac tgatgcacgg ccagcgggag accgggtccc
     4021 agcagaggag cctccggcca cagctcctct ccctgcccac accaaacatg gcagcagaga
     4081 cgggagcacc cagacggatg gccctgcaga caacacctct gcctgcttgg cctcagaacc
     4141 cgatggcctc ctggggtgca acagtagcca gaggacaccc tctctggact ctatagctgc
     4201 aaccagagtc caggatctgt cagacatggt agaaatactg atctgagata tccgatccac
     4261 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4321 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4381 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4501 ttaacgcgga tctgggcgtg gttaagggtg ggaaagaata tataaggtgg gggtcttatg
     4561 tagttttgta tctgttttgc agcagccgcc gccgccatga gcaccaactc gtttgatgga
     4621 agcattgtga gctcatattt gacaacgcgc atgcccccat gggccggggt gcgtcagaat
     4681 gtgatgggct ccagcattga tggtcgcccc gtcctgcccg caaactctac taccttgacc
     4741 tacgagaccg tgtctggaac gccgttggag actgcagcct ccgccgccgc ttcagccgct
     4801 gcagccaccg cccgcgggat tgtgactgac tttgctttcc tgagcccgct tgcaagcagt
     4861 gcagcttccc gttcatccgc ccgcgatgac aagttgacgg ctcttttggc acaattggat
     4921 tctttgaccc gggaacttaa tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt
     4981 tctgccctga aggcttcctc ccctcccaat gcggtttaaa acataaataa aaaaccagac
     5041 tctgtttgga tttggatcaa gcaagtgtct tgctgtcttt atttaggggt tttgcgcgcg
     5101 cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg
     5161 tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg
     5221 tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag
     5281 gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg
     5341 cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg
     5401 agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga
     5461 ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt
     5521 agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc
     5581 atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt
     5641 ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca
     5701 aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag
     5761 ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc
     5821 tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg
     5881 ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggc
     5941 tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg
     6001 ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg
     6061 cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc
     6121 gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc
     6181 tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc
     6241 tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca
     6301 gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg
     6361 ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg
     6421 cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct
     6481 tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg
     6541 cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc
     6601 cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca
     6661 ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac
     6721 gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggg agtttaaacg
     6781 aattcaatag cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca
     6841 ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag
     6901 gtaagctccg gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt
     6961 ttctgcataa acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta
     7021 caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt
     7081 aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag
     7141 tcataatgta agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc
     7201 gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca
     7261 taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct
     7321 gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag
     7381 ccataacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac
     7441 ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag
     7501 gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa
     7561 cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt
     7621 tcccacgtta cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac
     7681 tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc
     7741 caccccctca ttatcatatt ggcttcaatc caaaataagg tatattattg atgatgttaa
     7801 ttaacatgca tggatccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7861 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8461 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8701 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8761 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8941 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9121 tgccattgct gcagccatga gattatcaaa aaggatcttc acctagatcc ttttcacgta
     9181 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     9241 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     9301 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     9361 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     9421 ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat
     9481 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     9541 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     9601 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     9661 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     9721 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     9781 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     9841 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     9901 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     9961 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
    10021 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    10081 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    10141 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    10201 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    10261 gttcttctga attttgttaa aatttttgtt aaatcagctc attttttaac caataggccg
    10321 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    10381 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    10441 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    10501 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    10561 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    10621 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    10681 cgccgctaca gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca
    10741 tcaataatat accttatttt ggattgaagc caatatgata atgagggggt ggagtttgtg
    10801 acgtggcgcg gggcgtggga acggggcggg tgacgtagta gtgtggcgga agtgtgatgt
    10861 tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca aaagtgacgt ttttggtgtg
    10921 cgccggtgta cacaggaagt gacaattttc gcgcggtttt aggcggatgt tgtagtaaat
    10981 ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa aactgaataa gaggaagtga
    11041 aatctgaata attttgtgtt actcatagcg cgtaatactg taatagtaat caattacggg
    11101 gtcattagtt catagcccat atatggagtt ccg
//
