LOCUS       sequence-217955-        2845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19 backbone with S. pyogenes CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wright AV, Doudna JA
  TITLE     Protecting genome integrity during CRISPR immune adaptation.
  JOURNAL   Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 
            10.1038/nsmb.3289. Epub 2016 Sep 5.
  PUBMED    27595346
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3289";
            journalName: "Nat Struct Mol Biol"; date: "2016-10"; volume: "23"; 
            issue: "10"; pages: "876-883"
COMMENT     Sequence Label: pAW091
FEATURES             Location/Qualifiers
     source          1..2845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   96..131
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   162..197
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   228..263
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   294..329
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   360..395
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   426..461
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     rep_origin      complement(618..1206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(698..717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1377..2168,2169..2237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2000..2019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2238..2342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2410..2428
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2466..2488)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2588..2607
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2801..2823
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2815..2832
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2816..2832
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(2839..2845,1..95)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 aataaatgca gtaatacagg ggcttttcaa gactgaagtc tagctgagac aaatagtgcg
       61 attacaaaat tttttagaca aaaatagtct acgaggtttt agagctatgc tgttttgaat
      121 ggtcccaaaa ctgcgctggt tgatttcttc ttgcgctttt tgttttagag ctatgctgtt
      181 ttgaatggtc ccaaaactta tatgaacata actcaatttg taaaaaagtt ttagagctat
      241 gctgttttga atggtcccaa aacaggaata tccgcaataa ttaattgcgc tctgttttag
      301 agctatgctg ttttgaatgg tcccaaaaca gtgccgagga aaaattaggt gcgcttggcg
      361 ttttagagct atgctgtttt gaatggtccc aaaactaaat ttgtttagca ggtaaaccgt
      421 gctttgtttt agagctatgc tgttttgaat ggtcccaaaa cttcagcaca ctgagacttg
      481 ttgagttcca tgttttagag ctatgctgtt ttgaatggtc tccattcaac attgccgatg
      541 ataacttgag aaagagacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1021 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1261 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1321 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1381 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1441 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1501 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1561 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1621 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1681 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1741 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1801 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1861 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1921 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1981 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2041 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2101 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2161 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2221 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2281 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2341 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2401 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2461 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2521 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2581 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2641 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     2701 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2761 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2821 acgacggcca gtgaattcta ctctt
//
