LOCUS       sequence-217956-        5115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA
FEATURES             Location/Qualifiers
     source          1..5115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     polyA_signal    1186..1410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1650..1669)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1715..1770
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1769..1788)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2131..2147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2131..2147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2144..2166)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2155..2171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2179..2185,2186..2203,2204..2209))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2224..2245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2330..2464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2484..2618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2521..2540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2575..2594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2686..2703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2857..3445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2937..2956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3616..4407,4408..4476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4239..4258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4477..4581)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4612..4991
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgc atactcttta tgcccccggt ggatatgaca ttatgggata
      721 tctggaccag atcggcaacc ggccaaaccc gcaggtggaa ctgggccccg tggatacatc
      781 ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt acgcgagtga
      841 agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga attgccggtt
      901 tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg tggatagtaa
      961 cacaatcaat actatgcgca aggcaatcga caggaatgcc gaggtgcagg ttgaagtagt
     1021 caattttaaa aagaatggac agcgatttgt taatttcctg actatgatac ctgttaggga
     1081 cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaat ccggagggtc
     1141 tggctccgga tcaagtggtg gcagcggtac caagtctacg aattcctgtg ccttctagtt
     1201 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     1261 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1321 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     1381 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     1441 ctagggggta tccccacgcg ccctgtagcg tgtaccgctg ggagttcgta gacggaaaca
     1501 aacgcagaat ccaagcgcgg ccgcccagga tacatagatt accacaactc cgagcccttc
     1561 caccgctagc gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     1621 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     1681 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     1741 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     1801 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     1861 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     1921 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     1981 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     2041 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     2101 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2161 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2221 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2281 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     2341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2401 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     2461 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     2521 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2581 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     2641 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2701 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2761 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2821 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2881 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2941 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3001 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3061 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3121 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3181 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3241 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3301 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3361 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3421 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3481 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3541 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3601 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3661 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3721 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3781 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3841 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3901 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3961 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4021 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4081 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4141 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4201 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4261 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4321 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4381 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4441 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4501 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4561 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
     4621 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4681 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4741 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4801 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4861 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4921 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4981 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     5041 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     5101 gggggggggg ggcgc
//
