LOCUS       sequence-217982-        6807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez 
            F, Smith CL, Youle RJ
  TITLE     The role of dynamin-related protein 1, a mediator of mitochondrial 
            fission, in apoptosis.
  JOURNAL   Dev Cell. 2001 Oct;1(4):515-25.
  PUBMED    11703942
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell.";
            date: "2001-10"; volume: "1(4)"; pages: "515-25"
COMMENT     Sequence Label: pEYFP-C1-Drp1
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(667..669,670..672,673..1383)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(712..733)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(973..992)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1320..1341
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3649..3770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3686..3705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3740..3759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3777..4232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3914..3935)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4126..4145
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4259..4363
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4365..4722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4365..4556
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4388..4408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4573..4708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4757..5551
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4757..5551
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4811..4830)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5421..5440
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5739..5758)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5783..5830
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6159..6747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6648..6667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
      661 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      721 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      781 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      841 cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac
      901 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      961 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1021 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1081 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1141 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1201 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1261 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1321 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1381 aagtccggac tcagatctcg agctcaagct tcgaattcga tggaggcgct aattcctgtc
     1441 ataaacaagc tccaggacgt cttcaacacg gtgggcgccg acatcatcca gctgcctcaa
     1501 atcgtcgtag tgggaacgca gagcagcgga aagagctcag tgctagaaag cctggtgggg
     1561 agggacctgc ttcccagagg tactggaatt gtcacccgga gacctctcat tctgcaactg
     1621 gtccatgttt cacaagaaga taaacggaaa acaacaggag aagaaaatgg ggtggaagca
     1681 gaagaatggg gtaaatttct tcacaccaaa aataagcttt acacggattt tgatgaaatt
     1741 cgacaagaaa ttgaaaatga aacagaaaga atttcaggaa ataataaggg agtaagccct
     1801 gaaccaattc atcttaagat tttttcaccc aacgttgtca atttgacact tgtggatttg
     1861 ccaggaatga ccaaggtgcc tgtaggtgat caacctaagg atattgagct tcaaatcaga
     1921 gagctcattc ttcggttcat cagtaatcct aattccatta tcctcgctgt cactgctgct
     1981 aatacagata tggcaacatc agaggcactt aaaatttcaa gagaggtaga tccagatggt
     2041 cgcagaaccc tagctgtaat cactaaactt gatctcatgg atgcgggtac tgatgccatg
     2101 gatgtattga tgggaagggt tattccagtc aaacttggaa taattggagt agttaacagg
     2161 agccagctag atattaacaa caagaagagt gtaactgatt caatccgtga tgagtatgct
     2221 tttcttcaaa agaaatatcc atctctggcc aatagaaatg gaacaaagta tcttgctagg
     2281 actctaaaca ggttactgat gcatcacatc agagattgtt taccagagtt gaaaacaaga
     2341 ataaatgttc tagctgctca gtatcagtct cttctaaata gctacggtga acccgtggat
     2401 gataaaagtg ctactttact ccaacttatt accaaatttg ccacagaata ttgtaacact
     2461 attgaaggaa ctgcaaaata tattgaaact tcggagctat gcggtggtgc tagaatttgt
     2521 tatattttcc atgagacttt tgggcgaacc ttagaatctg ttgatccact tggtggcctt
     2581 aacactattg acattttgac tgccattaga aatgctactg gtcctcgtcc tgctttattt
     2641 gtgcctgagg tttcatttga gttactggtg aagcggcaaa tcaaacgtct agaagagccc
     2701 agcctccgct gtgtggaact ggttcatgag gaaatgcaaa ggatcattca gcactgtagc
     2761 aattacagta cacaggaatt gttacgattt cctaaacttc atgatgccat agttgaagtg
     2821 gtgacttgtc ttcttcgtaa aaggttgcct gttacaaatg aaatggtcca taacttagtg
     2881 gcaattgaac tggcttatat caacacaaaa catccagact ttgctgatgc ttgtgggcta
     2941 atgaacaata atatagagga acaaaggaga aacaggctag ccagagaatt accttcagct
     3001 gtatcacgag acaaggttgc atctggaggt ggtggggttg gagatggtgt tcaagaacca
     3061 accacaggca actggagagg aatgctgaaa acttcaaaag ctgaagagtt attagcagaa
     3121 gaaaaatcaa aacccattcc aattatgcca gccagtccac aaaaaggtca tgccgtgaac
     3181 ctgctagatg tgccagttcc tgttgcacga aaactatctg ctcgggaaca gcgagattgt
     3241 gaggttattg aacgactcat taaatcatat tttctcattg tcagaaagaa tattcaagac
     3301 agtgtgccaa aggcagtaat gcattttttg gttaatcatg tgaaagacac tcttcagagt
     3361 gagctagtag gccagctgta taaatcatcc ttattggatg atcttctgac agaatctgag
     3421 gacatggcac agcgcaggaa agaagcagct gatatgctaa aggcattaca aggagccagt
     3481 caaattattg ctgaaatccg ggagactcat ctttggtgag gatccaccgg atctagataa
     3541 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3601 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     3781 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3841 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3901 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3961 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     4021 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     4081 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4141 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4201 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     4261 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4321 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     4381 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4441 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4621 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4681 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4741 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4801 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4861 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4921 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4981 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5041 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5101 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5161 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5221 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5281 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5341 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5401 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5461 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5521 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5581 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5641 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5701 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5761 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5821 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5881 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5941 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     6001 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6301 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6781 gccttttgct ggccttttgc tcacatg
//
