LOCUS       sequence-217989-        6337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa251 of Cre recombinase fused to ABI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2601_pCAG-PV1-iCre-N251-L1-ABI-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2363..2386
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2387..2407
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2417..2641
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2881..2900)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2946..3001
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3000..3019)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3362..3378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3362..3378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3375..3397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3386..3402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3410..3416,3417..3434,3435..3440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3455..3476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3561..3695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3715..3849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3752..3771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3806..3825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3917..3934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4088..4676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4168..4187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4847..5638,5639..5707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5470..5489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5708..5812)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5843..6222
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ccaacctgct gactgtgcac caaaacctgc ctgccctccc
      721 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca
      781 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg
      841 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct
      901 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct
      961 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct
     1021 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct
     1081 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg
     1141 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc
     1201 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat
     1261 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct
     1321 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa
     1381 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctt ccggagggtc
     1441 tggctccgga tcaagtggtg gcagcggtac ccctttgtat ggttttactt cgatttgtgg
     1501 aagaagacct gagatggaag atgctgtttc gactatacca agattccttc aatcttcctc
     1561 tggttcgatg ttagatggtc ggtttgatcc tcaatccgcc gctcatttct tcggtgttta
     1621 cgacggccat ggcggttctc aggtagcgaa ctattgtaga gagaggatgc atttggcttt
     1681 ggcggaggag atagctaagg agaaaccgat gctctgcgat ggtgatacgt ggctggagaa
     1741 gtggaagaaa gctcttttca actcgttcct gagagttgac tcggagattg ggtcagttgc
     1801 gccggagacg gttgggtcaa cgtcggtggt tgccgttgtt ttcccgtctc acatcttcgt
     1861 cgctaactgc ggtgactcta gagccgttct ttgccgcggc aaaactgcac ttccattatc
     1921 cgttgaccat aaaccggata gagaagatga agctgcgagg attgaagccg caggagggaa
     1981 agtgattcag tggaatggag ctcgtgtttt cggtgttctc gccatgtcga gatccattgg
     2041 cgatagatac ttgaaaccat ccatcattcc tgatccggaa gtgacggctg tgaagagagt
     2101 aaaagaagat gattgtctga ttttggcgag tgacggggtt tgggatgtaa tgacggatga
     2161 agaagcgtgt gagatggcaa ggaagcggat tctcttgtgg cacaagaaaa acgcggtggc
     2221 tggggatgca tcgttgctcg cggatgagcg gagaaaggaa gggaaagatc ctgcggcgat
     2281 gtccgcggct gagtatttgt caaagctggc gatacagaga ggaagcaaag acaacataag
     2341 tgtggtggtg gttgatttga aggattacaa ggacgatgac gataagccca agaaaaagcg
     2401 gaaggtgtga gaattcctgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2461 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2521 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2581 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2641 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     2701 gtgtaccgct gggagttcgt agacggaaac aaacgcagaa tccaagcgcg gccgcccagg
     2761 atacatagat taccacaact ccgagccctt ccaccgctag cgcactcctc aggtgcaggc
     2821 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     2881 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     2941 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     3001 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     3061 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     3121 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     3181 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     3241 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     3301 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     3361 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3421 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3481 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     3541 caattagtca gcaaccatag tcccgcccct aactccgccc agttccgccc attctccgcc
     3601 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3661 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     3721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3841 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3901 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4501 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4861 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4921 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4981 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5041 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5101 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5161 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5221 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5281 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5341 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5401 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5461 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5521 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5581 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5641 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5701 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5761 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5821 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt
     5881 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     5941 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     6001 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     6061 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     6121 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     6181 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     6241 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     6301 ttttaattat tttgtgcagc gatgggggcg ggggggg
//
