LOCUS       sequence-21799-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Raingeaud J, Whitmarsh AJ, Barrett T, Derijard B, Davis RJ
  TITLE     MKK3- and MKK6-regulated gene expression is mediated by the p38 
            mitogen-activated protein kinase signal transduction pathway.
  JOURNAL   Mol Cell Biol. 1996 Mar . 16(3):1247-55.
  PUBMED    8622669
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1996 Mar . 16(3):1247-55."
COMMENT     Sequence Label: pCDNA3-Flag MKK6(K82A)
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt cggcagccaa catggactat aaggacgatg atgacaaatc
      121 tcagtcgaaa ggcaagaagc gaaaccctgg ccttaaaatt ccaaaagaag catttgaaca
      181 acctcagacc agttccacac cacctagaga tttagactcc aaggcttgca tttctattgg
      241 aaatcagaac tttgaggtga aggcagatga cctggagcct ataatggaac tgggacgagg
      301 tgcgtacggg gtggtggaga agatgcggca cgtgcccagc gggcagatca tggcagtggc
      361 gcggatccga gccacagtaa atagccagga acagaaacgg ctactgatgg atttggatat
      421 ttccatgagg acggtggact gtccattcac tggcaccttt tatggcgcac tggttcggga
      481 gggtgatgtg tggatctgca tggagctcat ggatacatca ctagataaat tctacaaaca
      541 agttattgat aaagggcaga caattccaga ggacatctta aggaaaatag cagtttctat
      601 tgtaaaagca ttagaacatt tacatagtaa gctgtctgtc attcacagag acgtcaagcc
      661 ttctaatgta ctcatcaatg ctctcggtca agtgaagatg tgcgattt
//
