LOCUS       sequence-217991-        9472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Induces expression of human MICA allele 009 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Ferrari de Andrade L, Tay RE, Pan D, Luoma AM, Ito Y, Badrinath S, 
            Tsoucas D, Franz B, May KF Jr, Harvey CJ, Kobold S, Pyrdol JW, Yoon 
            C, Yuan GC, Hodi FS, Dranoff G, Wucherpfennig KW
  TITLE     Antibody-mediated inhibition of MICA and MICB shedding promotes NK 
            cell-driven tumor immunity.
  JOURNAL   Science. 2018 Mar 30;359(6383):1537-1542. doi: 
            10.1126/science.aao0505.
  PUBMED    29599246
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-03-30- 30"; volume: "359"; 
            issue: "6383"; pages: "1537-1542"
COMMENT     Sequence Label: pHAGE-EF1a MICA*009-IRES-ZsGreen
FEATURES             Location/Qualifiers
     source          1..9472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    99..666
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    116..668
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(260..277)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     487..506
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             680..1375
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    1389..1977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1442..1462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1860..1871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2052..2285
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2404..2422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2490..2594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2595..2663,2664..3455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2813..2832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3626..4214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4115..4134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4368..4385
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4625..4646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4661..4666,4667..4684,4685..4691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4699..4715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4704..4726
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4723..4739
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4723..4739
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4789..4808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5000..5633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5680..5805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6302..6535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6720..6764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7057..7174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7222..8400
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7453..8391
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8348..8368
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cagttgggac gagtgaccac agggatgcca cacagctcgg atttcagcct ctgatgtcag
       61 ctcttgggtc cactggctcc actgagggcg cctagggatc cctccccccc ccctaacgtt
      121 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
      181 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
      241 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
      301 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
      361 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
      421 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
      481 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
      541 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
      601 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
      661 tgataatatg gccacacata tggcccagtc caagcacggc ctgaccaagg agatgaccat
      721 gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg gcgagggcat
      781 cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg gcggcccctt
      841 gcccttcgcc gaggacatct tgtccgccgc cttcatgtac ggcaaccgcg tgttcaccga
      901 gtacccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct acacctggga
      961 ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca tcaccgtgag
     1021 cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact tccccgccga
     1081 cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga agatcatccc
     1141 cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc tgaaggacgg
     1201 tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc cccgcaagat
     1261 gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg acgccaagaa
     1321 ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc cctgaatcga
     1381 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     1441 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     1501 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     1561 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     1621 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     1681 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     1741 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     1801 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     1861 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     1921 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     1981 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     2041 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2101 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2161 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2221 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2281 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     2341 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     2401 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     2461 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2521 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2581 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2641 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2701 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2761 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2821 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2881 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2941 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3001 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3061 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3121 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3181 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3241 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3301 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3361 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3421 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3481 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3541 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3601 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3661 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3721 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3781 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3841 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3901 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3961 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4021 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4081 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4141 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4201 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4261 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4321 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4381 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4441 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4501 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4561 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4621 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4681 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     4741 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4801 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     4861 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     4921 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     4981 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
     5041 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     5101 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     5161 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     5221 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     5281 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     5341 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     5401 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     5461 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5521 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5581 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     5641 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     5701 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     5761 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     5821 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     5881 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     5941 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     6001 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     6061 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     6121 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
     6181 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     6241 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     6301 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     6361 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     6421 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     6481 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     6541 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     6601 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     6661 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     6721 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     6781 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     6841 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     6901 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     6961 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     7021 atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     7081 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     7141 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     7201 tccagtttgg actagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc
     7261 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     7321 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     7381 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     7441 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     7501 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     7561 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     7621 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     7681 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     7741 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     7801 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     7861 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     7921 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     7981 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     8041 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     8101 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     8161 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     8221 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     8281 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     8341 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     8401 agcggccgca tggggctggg cccggtcttc ctgcttctgg ctggcatctt cccttttgca
     8461 cctccgggag ctgctgctga gccccacagt cttcgttata acctcacggt gctgtcctgg
     8521 gatggatctg tgcagtcagg gtttcttgct gaggtacatc tggatggtca gcccttcctg
     8581 cgctatgaca ggcagaaatg cagggcaaag ccccagggac agtgggcaga agatgtcctg
     8641 ggaaataaga catgggacag agagaccagg gacttgacag ggaacggaaa ggacctcagg
     8701 atgaccctgg ctcatatcaa ggaccagaaa gaaggcttgc attccctcca ggagattagg
     8761 gtctgtgaga tccatgaaga caacagcacc aggagctccc agcatttcta ctacgatggg
     8821 gagctcttcc tctcccaaaa cgtggagact gaggaatgga cagtgcccca gtcctccaga
     8881 gctcagacct tggccatgaa cgtcaggaat ttcttgaagg aagatgccat gaagaccaag
     8941 acacactatc acgctatgca tgcagactgc ctgcaggaac tacggcgata tctagaatcc
     9001 agcgtagtcc tgaggagaac agtgcccccc atggtgaatg tcacccgcag cgaggcctca
     9061 gagggcaaca tcaccgtgac atgcagggct tccagcttct atccccggaa tatcacactg
     9121 acctggcgtc aggatggggt atctttgagc cacgacaccc agcagtgggg ggatgtcctg
     9181 cctgatggga atggaaccta ccagacctgg gtggccacca ggatttgcca aggagaggag
     9241 cagaggttca cctgctacat ggaacacagc gggaatcaca gcactcaccc tgtgccctct
     9301 gggaaagtgc tggtgcttca gagtcattgg cagacattcc atgtttctgc tgttgctgct
     9361 gctgctgctg ctatttttgt tattattatt ttctatgtcc gttgttgtaa gaagaaaaca
     9421 tcagctgcag agggtccaga gctcgtgagc ctgcaggtcc tggatcaaca cc
//
