LOCUS       sequence-217993-        6005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6005)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2049..2729
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2851..2972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2888..2907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2942..2961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2979..3434)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3116..3137)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3328..3347
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3461..3565
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3567..3924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3567..3758
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3590..3610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3775..3910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3959..4753
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3959..4753
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4013..4032)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4623..4642
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4941..4960)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4985..5032
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5361..5949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5850..5869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tcggcagcac ttttctttca ttggggggaa
      721 ggcgtgagga aagtaccaaa cagcagcaga cttttaaact ttaaacagac aggtctgaga
      781 gcctgaactc tcatttttcc tttgacttca gcctccaagg agttccacca actttggcgc
      841 gccggcttca ctttcattaa gtgaaagaga ggtgcccaga catgggtgac tggagtgcct
      901 tggggaagct tctggacaag gtccaagcct actccaccgc tggagggaag gtgtggctgt
      961 cagtgctctt catattcaga atcctgctcc tggggacagc tgttgagtca gcttggggtg
     1021 atgaacagtc tgcctttcgc tgtaacactc aacaacctgg ctgcgaaaac gtctgctatg
     1081 acaagtcctt ccccatctct cacgtgcgct tctgggtcct tcagatcata ttcgtgtctg
     1141 tgcccacact cctgtacttg gcccatgtgt tctatgtgat gaggaaggaa gagaagctaa
     1201 acaagaaaga agaggagctc aaagtggccc agactgacgg ggtcaacgtg gagatgcacc
     1261 tgaagcagat tgaaatcaag aagttcaagt acgggattga agagcacggc aaggtgaaaa
     1321 tgaggggcgg cttgctgaga acctacatca tcagcatcct cttcaagtct gtcttcgagg
     1381 tggccttcct gctcatccag tggtacatct atgggttcag cttgagcgcg gtctacacct
     1441 gcaagagaga tccctgcccc caccaggtag actgcttcct ctcacgtccc acggagaaaa
     1501 ccatcttcat catcttcatg ctggtggtgt ccttggtgtc tctcgctttg aacatcattg
     1561 agctcttcta cgtcttcttc aagggcgtta aggatcgcgt gaagggaaga agcgatcctt
     1621 accacgccac cactggccca ctgagcccat caaaagactg cggatctcca aaatacgcct
     1681 acttcaatgg ctgctcctca ccaacggctc cactctcgcc tatgtctcct cctgggtaca
     1741 agctggttac tggtgacaga aacaattcct cgtgccgcaa ttacaacaag caagctagcg
     1801 agcaaaactg ggcgaactac agcgcagagc aaaatcgcat ggggcaggcc ggaagcacca
     1861 tctccaactc gcacgcccag ccgttcgatt tccccgacga caaccagaat gccaaaaaag
     1921 ttgctgctgg acatgaactc cagccattag ccatcgtgga ccaacgacct tccagcagag
     1981 ccagcagccg cgccagcagc aggcctcggc ctgatgacct ggagattgcg gatccaccgg
     2041 tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg
     2101 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
     2161 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
     2221 ccactgcatt cctgtacggc aacagggtat tcgccaaata tccagacaac atacaagact
     2281 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
     2341 ggggcatttg caacgccaga aacgacataa caatggaagg ggacactttc tataataaag
     2401 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga
     2461 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatgtag
     2521 agatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
     2581 aagctaagga gaagggtgtc aagttaccag gcgcccactt tgtggaccac tgcattgaga
     2641 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
     2701 ctggattgcc tgacaatgcc agacgataag cggccgcgac tctagatcat aatcagccat
     2761 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     2821 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     2881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2941 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt
     3001 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3061 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3121 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3181 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3241 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3301 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3361 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3421 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3481 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3541 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     3601 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3661 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3721 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3781 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3841 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3901 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     3961 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4021 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4081 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4141 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4201 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4261 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4321 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4381 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4441 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4501 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4561 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4621 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4681 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4741 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4801 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4861 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4921 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4981 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     5041 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     5101 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     5161 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5221 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5281 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5341 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5401 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5461 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5521 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5581 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5641 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5701 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5761 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5821 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5881 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5941 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6001 gctca
//
