LOCUS       sequence-218007-        3469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ARRET with approximately 130 telomere hexanucleotide repeats of 
            TTAGGG and TTGGGG variant sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3469)
  TITLE     telomeric repeat-containing RNA (TERRA and ARRET) plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR ARRET
FEATURES             Location/Qualifiers
     source          1..3469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1026..1125)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1142..1161)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1143..1161)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1166..1182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1166..1182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1284..1303)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1295..2104
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1362..1381)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2251..2839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2740..2759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2993..3010
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3169..3196)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3288..3374)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3423..3445
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3437..3454
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3438..3454
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ttaaaggaac caattcagtc
      121 gatcgactgg atcgatgaat tccgaaccct aaccctaacc ctaaccctaa ccctaaccct
      181 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      241 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaacccc
      301 aaccccaacc ccaaccccaa ccccaacccc aaccctaacc ccaaccccaa ccccaacccc
      361 aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
      421 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      481 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      541 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      601 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaacccc
      661 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      721 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      781 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      841 aaccctaacc ctaaccctaa ccctaaccct aaccccaacc ctaaccctaa ccctaacgct
      901 aaccctaacc ccaaccctaa ccccaacccc aaccccaacc ccaaccccaa ccggaattcc
      961 gcggaattcg agctcggtac ccggggatcg acaattccac tttttttaat tctcgagata
     1021 tctagaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     1081 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     1141 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1201 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1261 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1321 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1381 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     1441 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1501 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1561 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     1621 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1681 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1741 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     1801 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     1861 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     1921 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     1981 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2041 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2101 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2161 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2221 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2281 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2341 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2401 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2461 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2521 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2581 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2641 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2701 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2761 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2821 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2881 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2941 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3001 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3061 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3121 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
     3181 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     3241 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     3301 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     3361 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     3421 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
